Article
Oncology
Peng Zhu, Xiang Li, Yujie Liu, Jing Xiong, Ding Yuan, Yan Chen, Lili Luo, Ju Huang, Binbin Wang, Quanfang Nie, Shuli Wang, Liying Dang, Shu Li, Yan Shu, Wei Zhang, Honghao Zhou, Lan Fan, Qing Li
Summary: Cervical cancer (CC) is a major cause of cancer-related deaths globally. Early screening is crucial for preventing the progression of pre-malignant cervical intraepithelial neoplasia (CIN) to invasive cervical cancer. In this study, we found that endothelin 3 (EDN3) was downregulated and hypermethylated in cervical squamous cell carcinoma (CSCC). Further analysis showed that EDN3 expression decreased with the severity of cervical lesions, while methylation of the EDN3 gene increased. Treatment with a DNMT1 inhibitor reversed the methylation-mediated silencing of EDN3 in cervical cancer cell lines, leading to inhibition of cell proliferation, migration, and invasion. EDN3 may serve as a potential biomarker and therapeutic target for CSCC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Stefanie Dick, Danielle A. M. Heideman, Constantijne H. Mom, Chris J. L. M. Meijer, Johannes Berkhof, Renske D. M. Steenbergen, Maaike C. G. Bleeker
Summary: Women treated for CIN2/3 are still at risk of recurrent CIN and cervical cancer. This study evaluated the use of methylation markers ASCL1/LHX8 and FAM19A4/miR124-2 for detecting recurrent CIN2/3 after treatment. Methylation testing showed good performance in detecting recurrent CIN, and can help identify women in need of re-treatment. Monitoring posttreatment with methylation analysis can be beneficial in managing the risk of recurrent CIN.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Cell Biology
Samatha Bhat, Shama Prasada Kabekkodu, Divya Adiga, Rayzel Fernandes, Vaibhav Shukla, Poonam Bhandari, Deeksha Pandey, Krishna Sharan, Kapaettu Satyamoorthy
Summary: Cervical cancer is a major cause of cancer-related deaths in developing countries, with zinc-finger protein 471 (ZNF471) identified as a potential tumor suppressor through its epigenetic regulation and targeting of epithelial-mesenchymal transition signaling pathways. High methylation of ZNF471 and low expression are associated with poor prognosis, and ZNF471 overexpression inhibits tumor growth and key signaling pathways in vitro and in vivo.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Oncology
Maria Kyrgiou, Anna-Barbara Moscicki
Summary: The microbiome in the female reproductive tract plays a crucial role in maintaining health and homeostasis. Imbalances in the genital tract microbiome and specific bacteria and cytokines may contribute to the development of HPV infection and cervical intra-epithelial neoplasia, leading to cervical cancer.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Oncology
Smita Joshi, Richard Muwonge, Vinay Kulkarni, Mahesh Mandolkar, Eric Lucas, Sanjay Pujari, Rengaswamy Sankaranarayanan, Partha Basu
Summary: This study provides updated incidence rates of cervical intraepithelial neoplasia (CIN) among women living with HIV (WHIV), and examines disease outcomes and HPV status among women treated for CIN. The study found that women who cleared HPV infection had a significantly increased risk of incident CIN 2 or worse lesions, while those with persistent HPV infection had an even higher risk of such lesions. The study also highlights the importance of long-term surveillance and the possibility of extending screening intervals among HPV negative women.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Multidisciplinary Sciences
Xiaoyue Yang, Ying Chen, Mei Li, Weipei Zhu
Summary: ERBB3 gene mutation and methylation modification have extensive regulatory mechanisms on the microenvironment of cervical squamous cell carcinoma and endocervical adenocarcinoma. ERBB3 is more likely to be a carcinogenic factor rather than a prognostic factor for cervical cancer, and its carcinogenic mechanism is related to immune system suppression and RNA methylation. Methylation of ERBB3 may serve as an immunotherapy target in cervical squamous cell carcinoma and endocervical adenocarcinoma.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Carlos Contreras-Romero, Eloy-Andres Perez-Yepez, Antonio Daniel Martinez-Gutierrez, Alma Campos-Parra, Alejandro Zentella-Dehesa, Nadia Jacobo-Herrera, Cesar Lopez-Camarillo, Guillermo Corredor-Alonso, Jaime Martinez-Coronel, Mauricio Rodriguez-Dorantes, David Cantu de Leon, Carlos Perez-Plasencia
Summary: Cervical cancer remains one of the leading causes of mortality in women despite efforts in screening and early detection. This study identified specific gene methylation patterns associated with treatment response and prognosis, suggesting their potential as molecular markers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lei Zhao, Xiaohong Wu, Junnian Zheng, Dong Dong
Summary: In this study, an efficient workflow LCM-mu WGBS was introduced to profile the DNA methylome of circulating tumor cells from lung cancer patients. A unique DNA methylation signature of CTCs distinct from primary lung cancer tissues was identified, with promoter hypermethylation of epithelial genes as a hallmark of stable epithelial-mesenchymal transition. Furthermore, CTCs may exhibit a stemness-related feature during dissemination and metastasis, providing potential noninvasive CTC DNA methylation biomarkers for clinical diagnosis.
Article
Oncology
J. de Waard, A. Bhattacharya, M. T. de Boer, B. M. van Hemel, M. D. Esajas, K. M. Vermeulen, G. H. de Bock, E. Schuuring, G. B. A. Wisman
Summary: This study designed a methylation marker panel to detect CIN3+ in hrHPV-positive self-samples from the Dutch PBS as an alternative triage test for cytology.
CLINICAL EPIGENETICS
(2023)
Article
Oncology
Jaqueline Loaeza-Loaeza, Berenice Illades-Aguiar, Oscar del Moral-Hernandez, Yaneth Castro-Coronel, Marco A. Leyva-Vazquez, Roberto Dircio-Maldonado, Julio Ortiz-Ortiz, Daniel Hernandez-Sotelo
Summary: This study analyzed the methylation of 8 genes involved in maintaining cell normality and found that high CIMP is significantly associated with the risk of HSIL and CC.
CLINICAL EPIGENETICS
(2022)
Article
Oncology
Mingjun Ma, Jingfen Zhu, Yongbin Yang, Xiaoyun Wang, Yubiao Jin, Jiawen Zhang, Sufang Wu
Summary: Human papillomavirus (HPV) infection is the main cause of female precancerous lesions and cervical cancer. The development and application of HPV prophylactic vaccines have been recognized as a major effective intervention for the control of cervical lesions. This retrospective study investigated the clinical features and risk factors of non-9-valent vaccine covered HPV subtypes in cervical lesions. The findings provide valuable information for optimizing cervical cancer prevention in the post-vaccine era.
Article
Medicine, Research & Experimental
Donghyun D. Lee, Martin Komosa, Sumedha Sudhaman, Ricardo Leao, Cindy H. Zhang, Joana D. Apolonio, Thomas Hermanns, Peter J. Wild, Helmut Klocker, Farshad Nassiri, Gelareh Zadeh, Bill H. Diplas, Hai Yan, Steven Gallinger, Trevor J. Pugh, Vijay Ramaswamy, Michael D. Taylor, Pedro Castelo-Branco, Nuno Miguel Nunes, Uri Tabori
Summary: The research found that allele-specific DNA methylation within the TERT promoter plays a significant regulatory role in cancer, especially more common in adult cancers and enriched in tumors with TERT promoter mutations. This allele-specific DNA methylation is essential for the regulation of TERT expression in cancer.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
Panagiotis Giannos, Konstantinos S. Kechagias, Sarah Bowden, Neha Tabassum, Maria Paraskevaidi, Maria Kyrgiou
Summary: The study investigated gene markers associated with CIN and CC, with PCNA identified as a highly ranked hub gene in both sample types. Additionally, PCNA was found to be overexpressed in CC tissues and correlated with favorable prognosis and tumor purity in CC patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Aslam Shiraz, Nagayasu Egawa, Daniel M. Pelt, Robin Crawford, Adeline K. Nicholas, Veronika Romashova, Peter Sasieni, Heather Griffin, John Doorbar
Summary: A triage test that generates molecular maps of the cervical surface has been developed to improve the detection rate of high-grade disease. The spatial molecular mapping showed a sensitivity of 90% for detecting high-grade disease and AI-based analysis could aid in disease detection through automated flagging of biomarker-positive cells.
Article
Obstetrics & Gynecology
Evangelos Paraskevaidis, Antonios Athanasiou, Ilkka Kalliala, Anna Batistatou, Maria Paraskevaidi, Evripidis Bilirakis, Maria Nasioutziki, Minas Paschopoulos, Deirdre Lyons, Marc Arbyn, Margaret Cruickshank, Pierre Martin-Hirsch, Maria Kyrgiou
Summary: This study presents the experience of a single department with cervical cancer cases following previous excision of cervical intraepithelial neoplasia (CIN) and discusses potential pathogenesis. It was found that there may be a long interval between initial CIN treatment and cancer diagnosis, with unexpected cancer diagnoses despite normal post-treatment follow-up.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2021)
