Review
Plant Sciences
Capilla Mata-Perez, Inmaculada Sanchez-Vicente, Noelia Arteaga, Sara Gomez-Jimenez, Andrea Fuentes-Terron, Cylia Salima Oulebsir, Monica Calvo-Polanco, Cecilia Oliver, Oscar Lorenzo
Summary: Environmental conditions have a significant impact on plant growth and development. Understanding the mechanisms involved in plant adaptation to abiotic stresses is crucial for improving crop production. Recent studies have shown how plants respond to various stresses through signaling networks and post-translational modifications of biological molecules, such as S-nitrosylation of proteins.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Physiology
Kelsey S. Kalous, Sarah L. Wynia-Smith, Brian C. Smith
Summary: Increased sirtuin deacylase activity is associated with longer lifespan and lower susceptibility to aging-related diseases in eukaryotes, while decreased activity is linked to higher disease risk. Oxidative post-translational modifications such as cysteine nitrosation and glutathionylation can inhibit sirtuin deacylase activity, potentially providing a mechanistic link between cellular oxidants and age-related disease development.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alvaro Costa-Broseta, MariCruz Castillo, Jose Leon
Summary: Nitric oxide (NO) plays a complex role in plants, potentially negatively regulating its own biosynthesis and influencing the activity of nitrate reductases through induced post-translational modifications. The ubiquitylation of proteins following NO-induced PTMs may lead to proteasome-mediated degradation, affecting the assimilation of nitrate and production of NO under complex environmental conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Manuel A. Matamoros, Manuel Becana
Summary: Legumes can reduce the demand for nitrogen fertilizers and contribute to sustainable agriculture by fixing nitrogen in symbiotic root nodules. However, global changes may increase exposure to abiotic stresses and negatively impact crop production. The complex regulation of whole plant physiology and nitrogen fixation in legumes under abiotic stress involves redox-based protein post-translational modifications, which play critical roles in plant acclimation and stress tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Xuewei Song, Ting Wang, Yang Zhang, Jing-Quan Yu, Xiao-Jian Xia
Summary: S-nitrosoglutathione reductase (GSNOR) plays a critical role in regulating plant stress tolerance, especially under high temperature conditions. Suppressing GSNOR expression reduces the thermotolerance of tomato plants, which is associated with decreased accumulation of abscisic acid (ABA), salicylic acid (SA), activated mitogen-activated protein kinase (MAPK), and heat shock protein. GSNOR is also involved in the upregulation of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and apoplastic H2O2 production in response to high temperature. Maintaining a balanced interaction between S-nitrosothiol (SNO) and H2O2 is crucial for cellular redox homeostasis and thermotolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jiali Zhai, Yuanlin Liang, Senlin Zeng, Jinping Yan, Kunzhi Li, Huini Xu
Summary: In tomato plants, S-nitrosylation dependent on nitric oxide is crucial for the function of glutathione reductase under NaCl stress. It enhances salt tolerance by increasing the transcripts, enzyme activity, and S-nitrosylated level of GR, while regulating the oxidative state.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Campolo, Mauricio Mastrogiovanni, Michele Mariotti, Federico M. Issoglio, Dario Estrin, Per Hagglund, Tilman Grune, Michael J. Davies, Silvina Bartesaghi, Rafael Radi
Summary: Glutamine synthetase (GS) is an important enzyme that plays a crucial role in nitrogen metabolism. This study investigated the oxidative inactivation of human GS by peroxynitrite (ONOO-) in vitro. The results showed that ONOO- exposure led to a dose-dependent loss of GS activity and various oxidative modifications of the enzyme. Multiple tyrosine nitration sites and dityrosine cross-links were identified, but nitration of specific tyrosine residues alone was not responsible for enzyme inactivation. In addition, ONOO- induced GS aggregation and activity loss, with thiol oxidation being a key modification to elicit aggregation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Plant Sciences
Zsuzsanna Kolbert, Christian Lindermayr
Summary: The identification of S-nitrosation and tyrosine nitration sites in plant proteins has been achieved through mass spectrometry. To predict these modifications, various bioinformatics tools have been developed, providing a fast and efficient way to obtain information. Although computational tools do not take into account the three-dimensional protein structure, they still serve as a useful resource for experimental validation and comparison.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Tomohiro Nakamura, Chang-ki Oh, Xu Zhang, Stuart A. Lipton
Summary: Neurodegenerative disorders such as Alzheimer's and Parkinson's diseases are characterized by progressive degeneration of synapses and neurons, often attributed to accumulated misfolded/aggregated proteins. Excessive reactive oxygen and nitrogen species in the brain may contribute to protein misfolding, potentially exacerbated by genetic mutations and environmental factors. Understanding the role of reactive nitrogen species in post-translational modifications of proteins could lead to therapeutic interventions for neurodegenerative diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Plant Sciences
Heng Zhou, Jingjing Huang, Patrick Willems, Frank Van Breusegem, Yanjie Xie
Summary: Reactive electrophilic species are widely present in plant cells and play a role in specific redox-regulated signaling events. Redox signaling is known to regulate gene expression during various biological processes, including plant growth, development, and stress responses. Recent data has shown that transcription factors are a major target of cysteine thiol-based oxidative post-translational modifications, which can affect their activity and transmit redox information to the nucleus. This review discusses the progress made in characterizing these modifications, their biochemical properties, and their effects on plant transcription factors, as well as the underlying mechanism of redox regulation and the challenges that remain in understanding the redox regulation of plant gene expression.
TRENDS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Przemyslaw Kopec, Marcin Rapacz, Rajeev Arora
Summary: The potential link between the functional activation of CBF and cellular redox state has been investigated, suggesting that redox-mediated CBF activation may play a crucial role in the cold acclimation process as well as light signaling.
TRENDS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Review
Biochemistry & Molecular Biology
Francisco J. Corpas, Salvador Gonzalez-Gordo, Jose M. Palma
Summary: Peroxisomes are single-membrane subcellular compartments found in almost all eukaryotic cells, containing high levels of H2O2 and catalase, as well as NO and other reactive nitrogen species. NO can modify proteins in peroxisomes, affecting their function, and has signaling properties to modulate protein-protein interactions and peroxisomal functions, especially in adverse environmental conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Kapuganti Jagadis Gupta, Vemula Chandra Kaladhar, Teresa B. Fitzpatrick, Alisdair R. Fernie, Ian Max Moller, Gary J. Loake
Summary: Nitric oxide (NO) is an important signal molecule in plants, playing key roles in plant development and stress responses. This review focuses on the pathways involved in NO production and scavenging, and how they are integrated with cellular metabolism.
Article
Plant Sciences
Beimi Cui, Xiangren Ma, Yuan Li, Yu Zhou, Xiuyun Ju, Adil Hussain, Saima Umbreen, Bo Yuan, Anika Tabassum, Jibril Lubega, Weixing Shan, Gary J. Loake, Qiaona Pan
Summary: The research shows that nitric oxide plays a crucial role in plant resistance against Phytophthora, with both high and low levels of NO accumulation leading to reduced resistance. S-nitrosylation, regulated by S-nitrosoglutathione reductase 1, is an important pathway for NO bioactivity. Loss of function mutations in GSNOR1 disable important defense mechanisms in plants against Phytophthora.
MOLECULAR PLANT PATHOLOGY
(2021)