Article
Veterinary Sciences
Sujuan Chen, Zheng Feng, Hualu Sun, Ruonan Zhang, Tao Qin, Daxin Peng
Summary: The research shows that there is a correlation between the biofilm formation of Salmonella Enteritidis and its level of vertical transmission in chickens. The genes csgD and bcsA play a significant role in facilitating the vertical transmission of Salmonella Enteritidis in chickens.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Fereshteh Badie, Mahmood Saffari, Rezvan Moniri, Behrang Alani, Fatemeh Atoof, Ahmad Khorshidi, Mohammad Shayestehpour
Summary: The study evaluated the combined effects of temperature and pH on the expression of biofilm, stress, and virulence genes in Salmonella Enteritidis and Salmonella Typhimurium. The findings indicated that temperature and pH did not have a significant effect on the gene expression levels in these Salmonella strains.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mehdi Zarei, Mohsen Paknejad, Mohammad Hadi Eskandari
Summary: Chlorine treatment is a common and effective disinfection method in food-related environments, but insufficient concentrations can lead to sublethal oxidative stress in bacteria and alter their growth behavior. This study evaluated the effect of sublethal chlorine stress on the biofilm formation of Salmonella Enteritidis. The results showed that sublethal chlorine stress activated biofilm and quorum sensing-related genes in planktonic cells of S. Enteritidis, promoting biofilm formation. However, the effect of chlorine stress was not observed in subsequent generations of Salmonella biofilm cells. Overall, sublethal chlorine concentrations can enhance the biofilm-forming ability of S. Enteritidis.
Article
Food Science & Technology
Mehdi Zarei, Saeid Rahimi, Ali Fazlara, Sahar Elmi Anvari
Summary: The surfaces of poultry slaughterhouse equipment are major sources of Pseudomonas contamination, causing spoilage of poultry meat during refrigerated storage. Pseudomonas strains with high biofilm-forming ability were isolated from different surfaces, and their biofilm formation was studied. These strains produced biofilm on polystyrene microplates, and some strains were identified as P. fragi, P. fluorescens, P. lundensis, and P. cedrina. The ability of these strains to form biofilms with S. enteritidis depended on the timing of biofilm formation and the contribution of S. enteritidis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Jiaojiao Zhang, Debao Wang, Jinyue Sun, Zhilan Sun, Fang Liu, Lihui Du, Daoying Wang
Summary: The study showed that the combination of ultrasound and phenyllactic acid had a synergistic effect on inactivating Staphylococcus aureus and Salmonella enteritidis biofilm cells, leading to damages in cell membrane integrity and intracellular ATP leakage.
Article
Food Science & Technology
Narjes Basiri, Mehdi Zarei, Mohammad Kargar, Farshid Kafilzadeh
Summary: In recent years, plasma-activated water (PAW) has attracted attention in the food industry for its potential in microbial decontamination. This study investigated the effect of PAW on biofilm formation of Salmonella Enteritidis and found that it significantly reduced planktonic cells and inhibited biofilm development. These findings suggest that PAW could be a promising treatment to eliminate bacterial biofilms.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Md Ashrafudoulla, Kyung Won Na, Kye-Hwan Byun, Duk Hyun Kim, Jang Won Yoon, Md Furkanur Rahaman Mizan, Iksoon Kang, Sang-do Ha
Summary: This study isolated and identified 26 S. Enteritidis isolates from a chicken processing facility using PCR and PFGE, all of which were genetically identical and had potential impacts on human health and economic revenue. Continuous monitoring of the molecular and biofilm characteristics of S. Enteritidis is necessary to enhance food safety.
Article
Microbiology
Tania Gaviria-Cantin, Andres Felipe Vargas, Youssef El Mouali, Carlos Jonay Jimenez, Annika Cimdins-Ahne, Cristina Madrid, Ute Romling, Carlos Balsalobre
Summary: Biofilm formation of Salmonella typhimurium and Escherichia coli is a common ancient multicellular behavior with important roles in cell-cell and inter-organism interactions. The orphan transcriptional regulator CsgD plays a central role in the regulation of rdar morphotype expression, and GreA/GreB are required for the expression of csgD operon and the rdar morphotype.
Article
Biotechnology & Applied Microbiology
Karine Patrin Pontin, Karen Apellanis Borges, Thales Quedi Furian, Daiane Carvalho, Daiane Elisa Wilsmann, Henrique Ribeiro Piaggio Cardoso, Annelise Kopp Alves, Gabriela Zottis Chitolina, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Vladimir Pinheiro do Nascimento
Summary: The study confirmed the antimicrobial activity of copper surfaces against Salmonella biofilm and demonstrated uniform corrosion comparable to commonly used surfaces.
Article
Food Science & Technology
Shoukui He, Zeqiang Zhan, Chunlei Shi, Siyun Wang, Xianming Shi
Summary: The biofilm formation of Salmonella Enteritidis was assessed under sub-minimal inhibitory concentrations of ethanol. Ethanol at subinhibitory concentrations was found to stimulate biofilm formation, decrease swimming motility, and enhance cell auto-aggregation and quorum sensing. However, other cell characteristics were not significantly affected by ethanol.
Article
Food Science & Technology
Ali Yassoralipour, Jun Xian Wong, Wen Han Chow, Sook Ngoh Chen, Chee Hao Kuan, Eng Tong Phuah, Ji Tan, Tsun-Thai Chai
Summary: This study simulated and evaluated the formation and transmission of Listeria monocytogenes biofilms on plastic cutting boards. The biofilms were found to mature within 24 hours, with the strongest adhesion. The transfer rates of L. monocytogenes from contaminated cutting boards to cabbage, chicken fillet, and bread were determined during the cutting process. Scrubbing with a commercial dish-washing liquid was effective in reducing microbial concentrations, while tap water rinsing was less effective. The findings highlight the importance of proper cleaning and sanitization of cutting boards.
Article
Infectious Diseases
Yuliany Guillin, Marlon Caceres, Rodrigo Torres, Elena Stashenko, Claudia Ortiz
Summary: This study evaluated the antimicrobial and anti-biofilm potential of 15 essential oils on two Salmonella strains, with 4 oils showing antimicrobial activity and 6 oils exhibiting anti-biofilm activity. The EOs from Lippia origanoides chemotype thymol-carvacrol II (LTC II) had the lowest MIC and MBC values, along with the highest biofilm inhibition percentages on both strains. Transcriptional analysis revealed significant changes in gene expression related to quorum sensing and biofilm formation, indicating the potential of EOs as anti-biofilm antimicrobial agents. Further studies are required to elucidate the mechanisms of action of essential oils on the bacterial cells studied.
Article
Microbiology
Xia Meng, Mengping He, Binjie Chen, Pengpeng Xia, Jinqiu Wang, Chunhong Zhu, Heng Wang, Guoqiang Zhu
Summary: RyhB-1 and RyhB-2 are small non-coding RNAs that regulate iron homeostasis in Salmonella by sensing iron concentration in the environment. They inhibit the growth of Salmonella Typhimurium in macrophages by modulating the expression of genes sicA and rtsB. The regulatory role of RyhBs and their targets in Salmonella Enteritidis is not well understood. We found that RyhB paralogs are expressed when Salmonella Enteritidis interacts with chicken macrophages. They downregulate survival-related virulence factors and attenuate the survival of Salmonella Enteritidis inside macrophages.
Article
Food Science & Technology
Shoukui He, Beining Ye, Zengfeng Zhang, Yan Cui, Siyun Wang, Xianming Shi
Summary: This study investigated the effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis and the associated changes in fatty acid composition. The results showed that acid adaptation increased the ethanol tolerance of log-phase cells but decreased the tolerance of stationary-phase cells. Acid adaptation did not cause cell leakage or morphological changes but led to changes in fatty acid composition. Specifically, C16:0 fatty acid was involved in the protective effect of acid adaptation on ethanol tolerance.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Multidisciplinary Sciences
Rortana Chea, Hung Nguyen-Viet, Sothyra Tum, Fred Unger, Johanna Lindahl, Delia Grace, Chhay Ty, Sok Koam, Vor Sina, Huy Sokchea, Son Pov, Theng Heng, Or Phirum, Sinh Dang-Xuan
Summary: This study assesses the transfer of Salmonella enterica from raw chicken carcasses to ready-to-eat chicken salad in Cambodia. The results show how different hygiene practices influence the risk of pathogens contaminating chicken salad.
Article
Food Science & Technology
Stefani T. A. Dantas, Carlos H. Camargo, Monique R. Tiba-Casas, Ricardo C. Vivian, Jose P. A. N. Pinto, Jose C. F. Pantoja, Rodrigo T. Hernandes, Ary Fernandes Junior, Vera L. M. Rall
FOOD RESEARCH INTERNATIONAL
(2020)
Article
Agriculture, Dairy & Animal Science
Simony T. Guerra, Henrique Orsi, Samea F. Joaquim, Felipe F. Guimaraes, Bruna C. Lopes, Felipe M. Dalanezi, Domingos S. Leite, Helio Langoni, Jose C. F. Pantoja, Vera L. M. Rall, Rodrigo T. Hernandes, Simone B. Lucheis, Marcio G. Ribeiro
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Biochemical Research Methods
Lidiane Nunes Barbosa, Fernanda Cristina Bergamo Alves, Bruna Fernanda Murbach Teles Andrade, Mariana Albano, Vera Lucia Mores Rall, Ana Angelica Henrique Fernandes, Marilia Afonso Rabelo Buzalaf, Aline de Lima Leite, Leticia Gomes de Pontes, Lucilene Delazari dos Santos, Ary Fernandes Junior
JOURNAL OF PROTEOMICS
(2020)
Article
Immunology
Ana Flavia Marques Pereira, Mariana Albano, Fernanda Cristina Bergamo Alves, Bruna Fernanda Murbach Teles Andrade, Alessandra Furlanetto, Vera Lucia Mores Rall, Lucilene Delazari dos Santos, Ricardo de Oliveira Orsi, Ary Fernandes Junior
MICROBIAL PATHOGENESIS
(2020)
Article
Veterinary Sciences
Bruna F. Rossi, Erika C. R. Bonsaglia, Ivana G. Castilho, Stefani T. A. Dantas, Helio Langoni, Jose C. F. Pantoja, Ary Fernandes Junior, Juliano L. Goncalves, Marcos Santos, Rinaldo A. Mota, Vera L. M. Rall
BMC VETERINARY RESEARCH
(2020)
Article
Agriculture, Dairy & Animal Science
Bruna F. Rossi, Erika C. R. Bonsaglia, Jose C. F. Pantoja, Marcos Santos, Juliano L. Goncalves, Ary Fernandes Junior, Vera L. M. Rall
Summary: The study revealed a strong association between the agr type of Staphylococcus aureus and the type of mastitis, indicating that understanding the genetic profiles of S. aureus could serve as an additional tool in controlling this disease.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Larissa Martins, Juliano L. Goncalves, Renata F. Leite, Tiago Tomazi, Vera L. M. Rall, Marcos Santos
Summary: This study aimed to evaluate the association between antimicrobial use and antimicrobial resistance in relation to Streptococcus uberis causing clinical mastitis in dairy herds. The findings showed high resistance of Strep. uberis isolates to macrolides, tetracycline, and penicillin G, while lower resistance was observed for ceftiofur and enrofloxacin.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Anderson Clayton da Silva Abreu, Marcelo Falsarella Carazzolle, Bruna Lourenco Crippa, Giovana Rueda Barboza, Vera Lucia Mores Rall, Liliana de Oliveira Rocha, Nathalia Cristina Cirone Silva
Summary: By using NGS to analyze the 16S rRNA gene, this study characterized the bacterial diversity of Minas Frescal cheese in both organic and conventional production, identifying the most prevalent bacterial families and genera. The NGS tool proved to be useful for bacterial characterisation but did not determine the quantity of bacteria based on organic production.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Lais Anversa, Caroline D. Romani, Eliane S. Caria, Erika K. Saeki, Gabriel A. N. Nascentes, Maricene Garbelotti, Regina C. A. Stancari, Stefani T. A. Dantas, Vera L. M. Rall, Luciana S. Ruiz, Carlos H. Camargo, Virginia B. Richini-Pereira
Summary: This study evaluated the physicochemical and microbiological quality of dialysis water and dialysate samples from three haemodialysis centres in Bauru City, Brazil. The presence of non-fermenting gram-negative bacilli (NFGNB) in some samples was found, which may pose a risk to patient health. Therefore, the inclusion of control and monitoring measures for NFGNB in haemodialysis fluids is recommended to enhance the safety of the dialytic process.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Fernanda C. Campos, Ivana G. Castilho, Bruna F. Rossi, Erika C. R. Bonsaglia, Stefani T. A. Dantas, Regiane C. B. Dias, Ary Fernandes Junior, Rodrigo T. Hernandes, Carlos H. Camargo, Marcio G. Ribeiro, Jose C. F. Pantoja, Helio Langoni, Vera L. M. Rall
Summary: This study characterized 110 Mammary Pathogenic E. coli (MPEC) isolates from cows with clinical mastitis. The genetic profile of the MPEC isolates was highly heterogeneous, making it difficult to establish a common genetic profile for classification. Furthermore, the detection of extended spectrum beta-lactamase (ESBL) producing isolates poses a serious public health concern.
Article
Microbiology
Erika Carolina Romao Bonsaglia, Rodolfo S. Rossi, Giulia Latosinski, Bruna Fernanda Rossi, Fernanda Cristina Campos, Ary Fernandes Jr, Jose Carlos F. Pantoja, Vera Lucia Mores Rall
Summary: The aim of this study was to investigate the correlation between Streptococcus agalactiae, the main pathogen causing mastitis in dairy cows, and the genes related to adhesion and invasion in bovine mammary epithelial cells (BMEC) as well as biofilm formation. Results showed that although there was no statistical correlation between the presence of virulence genes and increased SCC or biofilm production, biological evidence was observed between increased SCC and biofilm production.
Article
Veterinary Sciences
Juliano L. Goncalves, Juliana Young, Renata de F. Leite, Carlos E. Fidelis, Priscila A. Trevisoli, Luiz L. Coutinho, Nathalia C. C. Silva, Roger Cue, Vera Lucia Mores Rall, Marcos dos Santos
Summary: The study evaluated the impact of selective dry cow therapy on bacterial diversity, indicating that treating healthy cows at dry-off with only teat sealant did not alter bacterial diversity but showed a higher abundance of potentially beneficial bacterial groups.
VETERINARY SCIENCES
(2022)