Article
Biochemistry & Molecular Biology
Marta Ksiazczyk, Bartlomiej Dudek, Maciej Kuczkowski, Robert O'Hara, Kamila Korzekwa, Anna Wzorek, Agnieszka Korzeniowska-Kowal, Mathew Upton, Adam Junka, Alina Wieliczko, Radoslaw Ratajszczak, Gabriela Bugla-Ploskonska
Summary: This study compared E. coli populations isolated from reptiles, birds, and mammals (humans) for the first time, revealing that most reptilian E. coli strains are nonpathogenic with a low frequency of virulence gene occurrence, differing from the uropathogenic and avian pathogenic strains. Additionally, phylogroups distribution was relatively homogeneous among tested mammalian and avian strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Anuj Rana, Akshay Dhanokar, K. Siddaardha Duggirala, Arvind Pratap Singh
Summary: This study systematically examined various plant-origin foods for the presence of Extraintestinal pathogenic Escherichia coli (ExPEC) and specific putative ExPEC pathotypes. The findings revealed the presence of ExPEC isolates and specific putative ExPEC pathotypes such as Uropa-thogenic E. coli and Septicemia-associated E. coli. The study also highlighted the presence of multidrug resistance in these ExPEC pathotypes, posing a potential threat to human health and food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Review
Microbiology
Valerio M. Sora, Gabriele Meroni, Piera A. Martino, Alessio Soggiu, Luigi Bonizzi, Alfonso Zecconi
Summary: The One Health approach highlights the importance of antimicrobial resistance in both public health and food animal production, with specific focus on the ExPEC strains of E. coli which may transfer resistance genes to other pathogenic bacteria, posing a serious threat to public health globally.
Article
Microbiology
Camille Chagneau, Clemence Massip, Nadege Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Celine Besson, Pauline Le Faouder, Nicolas Cenac, Marie-Paule Roth, Helene Coppin, Maxime Fontanie, Patricia Martin, Jean-Philippe Nougayrede, Eric Oswald
Summary: This study found the presence of the genotoxin Colibactin in the urine of patients with urinary tract infections, and demonstrated its genotoxicity in bladder cells. This discovery raises the hypothesis of a potential link between urinary tract infections and bladder cancer.
Article
Immunology
Jin Hu, Dongfang Wang, Xingfa Huang, Yang Yang, Xin Lian, Wenjun Wang, Xiao Xu, Yulan Liu
Summary: This study explores the role of TolC in the pathogenesis of ExPEC strain PPECC42 and finds that TolC is closely associated with the virulence of ExPEC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Catia Longhi, Linda Maurizi, Antonietta Lucia Conte, Massimiliano Marazzato, Antonella Comanducci, Mauro Nicoletti, Carlo Zagaglia
Summary: Multiple-antibiotic-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) is a common cause of nosocomial and community-acquired infections. The coexistence of antibiotic and heavy metal resistance in ExPEC strains is increasing, and the mechanisms for heavy metal tolerance are not fully understood.
Article
Microbiology
Bo Hou, Chen-Yan Wang, Shao-Wen Li, Lun-Jiang Zhou, Yong-Liang Che, Qiu-Yong Chen
Summary: The HicAB type II T-A system is abundant in various bacteria and archaea and plays important roles in stress response, virulence, and bacterial persistence. This study demonstrated that the deletion of hicAB significantly decreased the biofilm-forming ability of ExPEC, but the mutant still retained the ability to form curli fimbriae. Further analysis revealed differential gene expression associated with biofilm formation between the hicAB mutant and the parent strain, suggesting that the HicAB system is involved in ExPEC biofilm formation through regulation of outer membrane protein gene expression.
CURRENT MICROBIOLOGY
(2023)
Article
Infectious Diseases
Isabella F. S. Figueiredo, Lorena G. Araujo, Raissa G. Assuncao, Itaynara L. Dutra, Johnny R. Nascimento, Fabricia S. Rego, Carolina S. Rolim, Leylane S. R. Alves, Mariana A. Frazao, Samilly F. Cadete, Luis Claudio N. da Silva, Joicy C. de Sa, Eduardo M. de Sousa, Waldir P. Elias, Flavia R. F. Nascimento, Afonso G. Abreu
Summary: The study showed that cinnamaldehyde has potential in treating sepsis by reducing inflammation, increasing cell production in bone marrow and spleen, and improving survival rates in animals.
Article
Microbiology
Rodrigo H. S. Tanabe, Regiane C. B. Dias, Henrique Orsi, Daiany R. P. de Lira, Melissa A. Vieira, Luis F. dos Santos, Adriano M. Ferreira, Vera L. M. Rall, Alessandro L. Mondelli, Tania A. T. Gomes, Carlos H. Camargo, Rodrigo T. Hernandes
Summary: In this study, we characterized 112 uropathogenic Escherichia coli (UPEC) isolates from Botucatu, Brazil. We identified the presence of hybrid UPEC/DEC and observed antibiotic resistance. Our findings are important for understanding the pathogenicity and resistance profiles of these hybrid strains.
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Article
Immunology
Ying Yu, Bo Hu, Huanhuan Fan, Hailing Zhang, Shizhen Lian, Hongye Li, Shuangshuang Li, Xijun Yan, Shaohui Wang, Xue Bai
Summary: The study identified molecular epidemiology and biological characteristics of Escherichia coli associated with hemorrhagic pneumonia (HP) in mink from five Chinese provinces. Analysis included serotyping, antimicrobial susceptibility, detection of virulence genes, phylogenetic grouping, whole-genome sequencing, drug resistant gene, multilocus sequence typing (MLST), and biofilm formation assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hongyan Sun, Yuxuan Cao, Yexin Yang, Huan Li, Lujiang Qu
Summary: This study identified differentially expressed miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection. Among those miRNAs, gga-miR-455-5p was found to regulate the expression of type I interferons via targeting IRF2, contributing to the host defense against APEC infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xinming Pan, Rong Chen, Yating Zhang, Yinchu Zhu, Jin Zhao, Huochun Yao, Jiale Ma
Summary: ExPEC strain PU-1 possesses a robust ability to colonize within the host bloodstream and induce a low level of leukocytic activation. The study reveals that Vat(PU-1) and Tsh(PU-1), two SPATEs proteins, can interact with hemoglobin and degrade mucins of the host respiratory tract, leading to impaired chemotaxis and transmigration of leukocytes and inhibition of immune responses, thus facilitating the colonization of ExPEC within the bloodstream.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Microbiology
Hicham Bessaiah, Carole Anamale, Jacqueline Sung, Charles M. Dozois
Summary: Pathogens are exposed to harmful conditions in the host environment, and bacterial responses play a crucial role in colonization and pathogenesis. Type 1 fimbriae in E. coli are important colonization factors and their regulation is influenced by signaling and stress. The regulation of type 1 fimbriae is potentially linked to environmental stress responses, and plays a role in regulating colonization and virulence of extraintestinal pathogenic E. coli.
Article
Food Science & Technology
Shiowshuh Sheen, Chi-Yun Huang, Shihyu Chuang
Summary: The study successfully reduced pathogenic bacteria in meat by combining high hydrostatic pressure technology and food-grade additives, achieving a 5-log reduction and demonstrating a synergistic effect. The developed models help processors find optimal processing parameters to meet food safety requirements.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Ewelina Bigoraj, Iwona Kozyra, Agnieszka Kaupke, Zbigniew Osinski, James Lowther, Artur Rzezutka
Summary: The aim of this study was to assess the prevalence of human enteric viruses in mussels sold on the Polish market and study the fecal contamination of shellfish. The study found that human enteric viruses were commonly present in shellfish, with norovirus being the most frequently detected. The study also observed a seasonal distribution pattern of viruses in shellfish samples.
Editorial Material
Food Science & Technology
Bojan Blagojevic, Lis Alban Denmark
Article
Food Science & Technology
Indy Magnus, Fatemeh Abbasi, Hugo Thienpont, Lien Smeesters
Summary: Food safety and quality are increasingly important in our society, driving the development of novel optical food sensing technologies. However, current optical sensing technologies are often limited in their sensitivity and product variability. In this study, we developed a novel pistachio nut screening methodology using fluorescence spectroscopy and advanced chemometrics to simultaneously detect shells, tree parts, and aflatoxins. By optimizing hardware and software parameters, we achieved a high classification accuracy for healthy pistachio kernels.
Article
Food Science & Technology
Xiaodong Wang, Jingqiang Yu, Haixia Wu, Leiying Wu, Wuyunga Borjihan, Cong Wang, Munkhjargal Burenjargal, Alideertu Dong
Summary: This study developed a novel photon-phonon coupling (PPC) cold sterilization technology and compared its effects with low-temperature pasteurization and microwave ultraviolet. The results showed that PPC effectively reduced bacterial count in raw milk with minimal impact on milk quality and nutritional composition.
Review
Food Science & Technology
Riyadh Abdulmalek Hassan, Sharina Abu Hanifah, Lee Yook Heng, Fawaz Al-badaii, Alizar Ulianas
Summary: This study used bibliometric visualisation map analysis to explore the publication outputs, trends, and hotspots for endocrine disruptors (EDMs) biosensor development in relation to mycotoxins. The analysis showed that the current literature primarily focuses on developing biosensors for detecting highly toxic and widely distributed mycotoxins in food, but there needs to be more focus on biosensors for detecting mycotoxins known as endocrine disruptors.
Article
Food Science & Technology
Yajie Zheng, Jianpeng Dou, Changpo Sun, Hujun Liu, Shipei Zhang, Wenfu Wu
Summary: This study investigated the efficiency of modified SA/ll-glucan/Mt microsphere adsorbents in removing DON and ZEN from CSL. The microspheres showed high adsorption capacity, and simple and rapid regeneration was achieved without affecting the removal efficiency of mycotoxins from CSL.
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Article
Food Science & Technology
Jia-Wen Wei, Jia-Rong He, Shi-Yi Chen, Yi-Han Guo, Xuan-Zhu Huo, Nuan Zheng, Shuo-Hui Cao, Yao-Qun Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are a major cause of human cancer. This study developed a quantitative analysis method for PAH4 using the back propagation neural network (BPNN) algorithm and constant wavelength synchronous fluorescence (CWSF) spectra as the data sets. The method can predict the concentrations of PAH4 in edible oil samples without preprocessing or pre-separation. It has been proven to be a powerful tool for the rapid detection of PAH4.
Article
Food Science & Technology
Liyun Chen, Xiumei Li, Jingyi Chen, Ranxun Lin, Yuhan Mai, Yuxin Lin, Guodong Wang, Zheng Chen, Wei Zhang, Jiang Wang, Cai Yuan, Longguang Jiang, Peng Xu, Mingdong Huang
Summary: Food spoilage is primarily caused by microbial contamination. Curcumin has shown moderate antimicrobial activity as a food preservative. This study formulates curcumin with zinc acetate to enhance its efficacy, resulting in Cur-Zn with increased antimicrobial activity against multiple food-contaminating bacteria and fungi. With light illumination, the antimicrobial activity of Cur-Zn is further enhanced due to the photodynamic inactivation (PDI) effect.
Article
Food Science & Technology
Marta Pilar Ortiz-Moriano, Gonzalo Machado-Schiaffino, Eva Garcia-Vazquez, Alba Ardura
Summary: Mislabelling in the global fisheries sector raises concerns about the identity, safety, and sustainability of seafood products. This study examined mislabelling in shrimps and prawns sold in the Spanish market and assessed potential risks of heavy metal ingestion.
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Suwimol Surareungchai, Chaleeda Borompichaichartkul, Chitsiri Rachtanapun, Nutthachai Pongprasert, Pongphen Jitareerat, Varit Srilaong
Summary: This study builds upon previous research and focuses on risk assessment and analysis in the organic salad industry. The results show that previous risk matrix models can be adapted for various raw materials, and preventive control plans can be applied across different aspects of the production process.
Article
Food Science & Technology
Xuan Zhang, Changling Fang, Xiaoyi Lou, Feng Han, Siman Li, Yongfu Shi, Dongmei Huang
Summary: This study aimed to assess the levels of Biogenic amines (BAs) in four common food types in the Chinese market. It was found that cheese and seafood derived product showed higher levels of BAs, and the histamine level in mackerel and yellow rice wine exceeded the limits.
Article
Food Science & Technology
Martina Foschi, Alessandra Biancolillo, Federico Marini, Francesco Cosentino, Francesca Di Donato, Angelo Antonio D'Archivio
Summary: This study aimed to develop a potentially non-destructive and routine-compatible method for the geographical traceability of high-valued Italian chickpeas. By analyzing the spectral information of the outer and inner parts of the kernels, it was found that the spectral analysis of the outer part yielded accurate and reliable results, indicating the possibility of non-destructive authentication.