Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 116, Issue -, Pages 315-322Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2018.04.053
Keywords
Myricetin; Colon cancer; Apoptosis; Caspase-related proteins; Nucleoside diphosphate kinase (NDPK)
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Funding
- Business for Cooperative R&D between Industry, Academy, and Research Institutes - Korean Small and Medium Business Administration [C0443066]
- gs2:National Research Foundation of Korea - Ministry of Education, Science and Technology [NRF-2014R1A6A3A04054307]
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [C0443066] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The flavonoid myricetin (MYR) is derived from vegetables and fruits. It has been shown to exert anti-cancer effects in various cell lines; however, the exact mechanism underlying these effects is yet to be elucidated. In this study, we evaluated the anti-cancer effects induced by MYR treatment in colon cancer HCT-15 cells. To detect cell proliferation, we conducted MU assay and real time-cell electronic sensing (RT-CES). We next performed comet assay and Annexin V and PI staining to detect cellular apoptotic features. After that, we conducted two-dimensional electrophoresis (2-DE) analysis to identify apoptotic proteins. The results of this analysis revealed that eight spots were differentially expressed. Among the spots, we selected nucleoside diphosphate kinase (NDPK) for further investigation, as it has been shown to regulate cancer cell apoptosis and metastasis. After that, we conducted realtime-PCR and western blot to detect the expression of NDPK mRNA and protein and wound-healing assay to detect cell migration and invasion. Finally, we performed NDPK siRNA transfection study and the results showed that NDPK knockdown inhibited apoptosis. Based on these collective results, we suggest that MYR induces apoptosis in human colon cancer HCT-15 cells selectively by increasing the expression of NDPK and other caspase-regulated apoptosis proteins.
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