Review
Obstetrics & Gynecology
Nuria Pellicer, Mauro Cozzolino, Cesar Diaz-Garcia, Daniela Galliano, Ana Cobo, Antonio Pellicer, Sonia Herraiz
Summary: The ovary has a short functional lifespan and preserving ovarian function is important for women with threats to ovarian reserve. Techniques such as ovarian fragmentation, stem cells or platelet-rich plasma show promising outcomes in regaining ovarian function. However, there is limited clinical evidence for their widespread use and research should focus on increasing follicle number and quality.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Endocrinology & Metabolism
Xiaojun Kuang, Yongzhe Tang, Hong Xu, Min Ji, Dongmei Lai
Summary: This review assesses the effectiveness of various therapies in recovering ovarian function in patients with primary ovarian insufficiency (POI) and explores a comprehensive evaluation method. The results indicate a lack of scientific evidence supporting any therapy in effectively restoring ovarian function in patients with POI.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Sairah Sheikh, Belinda K. M. Lo, Heidy Kaune, Jassimran Bansal, Anna Deleva, Suzannah A. Williams
Summary: The mechanisms and aetiology of premature ovarian insufficiency (POI) are still unclear, but the oocyte plays a significant role. The Double Mutant (DM) mouse model showed that oocyte-specific deletion of certain glycans leads to ovarian dysfunction and eventually POI. This study demonstrates that abnormal communication between granulosa cells (GCs) and oocytes results in the development of abnormal primary follicles and the onset of POI.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Robabe Hosseinisadat, Alireza Farsi Nejad, Frough Mohammadi
Summary: This study aimed to evaluate the effect of intra-ovarian infusion of platelet-rich plasma (PRP) in treating women with poor ovarian reserve. The results showed that PRP can significantly increase the levels of anti-Mullerian hormone (AMH) and improve fertility in these women.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Bingying Xu, Zhuqing Li, Shan Li, Hanni Ke, Qian Zhang, Yingying Qin, Ting Guo
Summary: This study identified pathogenic variants of the TSC2 gene in patients with POI and suggested the involvement of defective TSC/mTOR pathway-mediated primordial follicle hyperactivation in the pathogenesis of POI.
FERTILITY AND STERILITY
(2022)
Article
Pharmacology & Pharmacy
Chao Zhang, YuanHong Li, Jie Qin, ChengQian Yu, Gang Ma, HongDuo Chen, XueGang Xu
Summary: In this study, it was found that injection of BMSC and MSC-CM led to the transition of telogen hair follicles to anagen hair follicles, and enhanced proliferation of HFSCs positive for Krt15 and Sox9 was observed. Proteomics analyses identified Krt25, Cpm, Stmn1, and Mb as potentially central players in hair follicle transition, suggesting they could be viable clinical targets for HL treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Oren Kashi, Dror Meirow
Summary: This manuscript reviews the impact of chemotherapy on ovarian reserve and the preventive measures. Chemotherapy can deplete dormant follicles and potentially lead to ovarian insufficiency. Ideal drugs for fertility preservation can be found by intervening in ovarian toxic mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Su Mi Kim, Jung Young Yoo, Yeon Hee Hong, Jaewang Lee, Ji Hyang Kim, Jung Ryeol Lee
Summary: This study aimed to evaluate the effects and mechanisms of action of growth hormone (GH) on the recovery of ovarian function in a mouse model of ovarian insufficiency induced by cyclophosphamide (CP). The results showed that GH treatment protected primordial follicles, improved follicular quality, enhanced in vitro fertilization outcomes, reduced apoptosis and stromal fibrosis, and increased angiogenesis by upregulating angiogenesis-related genes such as Leptin, platelet endothelial cell adhesion molecule 1, and angiogenin.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Chia Lin Chang
Summary: The decline in fertility in aging women, especially those with poor ovarian response (POR) or primary ovarian insufficiency (POI), is a major concern. Researchers have explored alternative approaches, including adjunct interventions and mechanical procedures, to improve fertility outcomes. However, the molecular mechanisms underlying fertility decline in aging women are not yet fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jimin Han, Kaijun Lin, HuiQin Choo, Yu Chen, Xuezheng Zhang, Ren-He Xu, Xusheng Weng, Yaojiong Wu
Summary: This study used multiple lineage tracing analysis to investigate the contribution of different hair follicle stem cell populations to sebaceous gland regeneration. The results showed that upper bulge stem cells played a significant role in sebaceous gland replenishment during the hair cycle, while lower bulge stem cells did not. During skin wound healing, all hair follicle stem cell populations participated in sebaceous gland replenishment.
Article
Medicine, Research & Experimental
Sichen Zhang, Dashuai Zhu, Zhenhua Li, Ke Huang, Shiqi Hu, Halle Lutz, Mengjie Xie, Xuan Mei, Junlang Li, Genevieve Neal-Perry, Shaowei Wang, Ke Cheng
Summary: The therapeutic ovarian regenerative patch (ORP) has been found to effectively treat POI and restore fertility in rats by stimulating the proliferation of ovarian somatic cells and inhibiting apoptosis under injury stress. This cell-free, off-the-shelf, and clinically feasible treatment represents a promising approach for POI.
Review
Endocrinology & Metabolism
Xiaodi Pu, Leisheng Zhang, Peiyu Zhang, Yaqiong Xu, Jun Wang, Xiaomei Zhao, Zhihua Dai, Hua Zhou, Shuyun Zhao, Anran Fan
Summary: Premature ovarian insufficiency (POI) induced by chemotherapy is a difficult-to-treat disorder that affects female fertility. The use of human umbilical cord mesenchymal stem cells (hUCMSCs) and exosomes derived from them shows potential in improving ovarian function and protecting fertility in POI patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Jinki Cho, Tae-Hee Kim, Jin Seok, Ji Hye Jun, Hyeri Park, Minyeoung Kweon, Ja-Yun Lim, Gi Jin Kim
Summary: Vascular endothelial growth factor secreted by placenta-derived mesenchymal stem cells (PD-MSCs) promotes follicular development and ovarian function after ovariectomy through vascular remodeling. These results provide fundamental data for understanding the therapeutic mechanisms of stem cell therapy based on placenta-derived mesenchymal stem cells PD-MSCs and provide a theoretical foundation for their application for obstetrical and gynecological diseases, including infertility and menopause.
LABORATORY INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Feixiang Chen, Qiang Zhang, Ping Wu, Yanan Zhao, Xiaotong Suo, Ao Xiao, Meifang Ke, Xiaohua He, Zan Tong, Yun Chen
Summary: A novel seedbed-like scaffold fabricated using Flammulina velutipes showed excellent biological activity and water absorption capacity after further optimization processing. The Flammulina velutipes polysaccharides-derived scaffolds exhibited remarkable antibacterial activity against various bacteria and promoted accelerated wound healing and hair follicle regeneration in a rat skin wound model.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pharmacology & Pharmacy
Ying Peng, Lu Sun, Wentong Guo, Zhigang Liu, Tianxiang Wang, Tingfeng Zou, Jie Zhou, Xiaoxiao Yang, Xiaodong Fan
Summary: In this study, the role of berberine (BBR) on ovarian function decline in a premature ovarian insufficiency (POI) mouse model was investigated. The results showed that BBR can effectively improve ovarian function by reducing oxidative stress and inflammatory response.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2023)
Article
Obstetrics & Gynecology
Cesar Diaz-Garcia, Sonia Herraiz, Loida Pamplona, Jessica Subira, Maria Jose Soriano, Carlos Simon, Emre Seli, Antonio Pellicer
Summary: Ovarian fragmentation for follicular activation increases antral follicle counts in women with poor ovarian response, but does not significantly modify in vitro fertilization outcomes.
FERTILITY AND STERILITY
(2022)
Article
Biochemistry & Molecular Biology
Alba Machado-Lopez, Roberto Alonso, Victor Lago, Jorge Jimenez-Almazan, Marta Garcia, Javier Monleon, Susana Lopez, Francisco Barcelo, Amparo Torroba, Sebastian Ortiz, Santiago Domingo, Carlos Simon, Aymara Mas
Summary: The absence of standardized molecular profiling to differentiate uterine leiomyosarcomas from leiomyomas is a current diagnostic challenge. In this study, artificial intelligence was used to search for a differential molecular signature for these myometrial tumors. Differential exome and transcriptome-wide research revealed higher tumor mutation burden in leiomyosarcomas compared to leiomyomas, as well as alterations in specific copy number variant regions and differentially expressed genes. A machine learning approach based on differentially expressed genes was developed to differentiate both tumor types with high sensitivity and specificity. These findings provide a novel molecular signature for the diagnosis of leiomyoma and leiomyosarcoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Antonio Capalbo, Silvia Buonaiuto, Matteo Figliuzzi, Gianluca Damaggio, Laura Girardi, Silvia Caroselli, Maurizio Poli, Cristina Patassini, Murat Cetinkaya, Beril Yuksel, Ajuna Azad, Marie Louise Grondahl, Eva R. Hoffmann, Carlos Simon, Vincenza Colonna, Semra Kahraman
Summary: This study identified new and known genes associated with infertility through case selection and whole-exome sequencing analysis, providing important clues for further research.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Review
Obstetrics & Gynecology
D. Leigh, D. S. Cram, S. Rechitsky, A. Handyside, D. Wells, S. Munne, S. Kahraman, J. Grifo, M. Katz-Jaffe, C. Rubio, M. Viotti, E. Forman, K. Xu, T. Gordon, S. Madjunkova, J. Qiao, Z-J Chen, G. Harton, L. Gianaroli, C. Simon, R. Scott, J. L. Simpson, A. Kuliev
Summary: Chromosome testing strategies, such as PGT-A, improve initial IVF outcomes by avoiding transfer of aneuploid embryos. Newer technologies have revealed trophectoderm mosaicism in embryos with intermediate copy number results. The transfer of mosaic embryos may be associated with increased implantation failure and miscarriage rates.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Letter
Obstetrics & Gynecology
Antonio Capalbo, Danilo Cimadomo, Laura Rienzi, Juan Antonio Garcia-Velasco, Carlos Simon, Filippo Maria Ubaldi
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
Maria Marchante, Anna Buigues, Noelia Ramirez-Martin, Jessica Martinez, Nuria Pellicer, Antonio Pellicer, Sonia Herraiz
Summary: Local administration of stem cells combined with activated platelet-rich plasma can restore ovarian function, improve oocyte quality, and enhance early embryo development in older mice. This study provides a potential treatment option to improve reproductive outcomes in women with age-related infertility.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Letter
Obstetrics & Gynecology
Carlos Simon, Maria Ruiz-Alonso, Carlos Gomez, Diana Valbuena
FERTILITY AND STERILITY
(2023)
Review
Obstetrics & Gynecology
Nuria Pellicer, Mauro Cozzolino, Cesar Diaz-Garcia, Daniela Galliano, Ana Cobo, Antonio Pellicer, Sonia Herraiz
Summary: The ovary has a short functional lifespan and preserving ovarian function is important for women with threats to ovarian reserve. Techniques such as ovarian fragmentation, stem cells or platelet-rich plasma show promising outcomes in regaining ovarian function. However, there is limited clinical evidence for their widespread use and research should focus on increasing follicle number and quality.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Medicine, Research & Experimental
Javier Gonzalez Fernandez, Javier Moncayo Arlandi, Ana Ochando, Carlos Simon, Felipe Vilella
Summary: Embryo-maternal cross-talk is crucial for embryo development and implantation, involving secreted factors and extracellular vesicles (EVs). EV cargo influences target cells and prepares them for reproductive stages like embryo development and implantation. Recent research suggests that EVs and their cargo can be used as a powerful, non-invasive tool to assess the quality of embryos and maternal tissues in assisted reproduction treatments. This review focuses on intercellular cross-talk between embryos and maternal tissues, examining human biology and signaling mechanisms identified in animal models.
Article
Obstetrics & Gynecology
Stephanie Bissiere, Blake Hernandez, Carmen Rubio, Carlos Simon, Nicolas Plachta
Summary: Preimplantation development is the stage of human development that can be observed and studied in real time outside the body. This is possible because human embryos can be created using in vitro fertilization and cultured until the blastocyst stage. This article focuses on the cellular and morphogenetic processes that transform a 1-cell embryo into a blastocyst ready for implantation. While most of our understanding of preimplantation human development comes from studying mouse embryos, this article discusses the potential differences in humans and highlights new approaches for studying this process using noninvasive imaging and genetic technologies.
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Laura Girardi, Matteo Figliuzzi, Maurizio Poli, Munevver Serdarogullari, Cristina Patassini, Silvia Caroselli, Ilaria Pergher, Francesco Cogo, Onder Coban, Fazilet Kubra Boynukalin, Mustafa Bahceci, Roser Navarro, Carmen Rubio, Necati Findikli, Carlos Simon, Antonio Capalbo
Summary: How well can whole chromosome copy number analysis from a single trophectoderm biopsy predict true mosaicism configurations in human blastocysts? When a single trophectoderm biopsy is tested, wide mosaicism thresholds increase false positive calls compared to more stringent ones without improving true detection rate, while binary classification provides the highest diagnostic accuracy.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
S. Caroselli, M. Figliuzzi, L. Picchetta, F. Cogo, P. Zambon, I Pergher, L. Girardi, C. Patassini, M. Poli, D. Bakalova, D. Cimadomo, N. Findikli, O. Coban, M. Serdarogullari, F. Favero, S. Bortolato, A. Anastasi, F. Capodanno, A. Gallinelli, F. Brancati, L. Rienzi, F. M. Ubaldi, J. Jimenez-Almazan, D. Blesa-Jarque, J. Miravet-Valenciano, C. Rubio, C. Simon, A. Capalbo
Summary: This study developed an integrated genetic testing platform that accurately detects chromosomal abnormalities, including ploidy level and microdeletions, expanding the clinical utility of preimplantation genetic testing.
HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Jose Ramon Hernandez Mora, Claudia Buhigas, Stephen Clark, Raquel Del Gallego Bonilla, Dagne Daskeviciute, Ana Monteagudo-Sanchez, Maria Eugenia Poo-Llanillo, Jose Vicente Medrano, Carlos Simon, Marcos Meseguer, Gavin Kelsey, David Monk
Summary: During pre-implantation stages, stored material in mammalian development promotes epigenetic profile erasure and genome activation. Through single-cell methylome and transcriptome sequencing, we analyzed the expression and methylation in oocytes and human embryos, identifying key events in epigenetic reprogramming and genome activation. Failure to achieve these milestones negatively impacts the developmental potential of embryos and may affect the success of assisted reproductive cycles.
Article
Biochemistry & Molecular Biology
Ana Domingo-Muelas, Robin M. Skory, Adam A. Moverley, Goli Ardestani, Oz Pomp, Carmen Rubio, Piotr Tetlak, Blake Hernandez, Eric A. Rhon-Calderon, Luis Navarro-Sanchez, Carmen M. Garcia-Pascual, Stephanie Bissiere, Marisa S. Bartolomei, Denny Sakkas, Carlos Simon, Nicolas Plachta
Summary: Combining fluorescent dyes with live imaging, this study reveals the dynamics of early development in human embryos, including chromosome segregation, compaction, polarization, blastocyst formation, and hatching. The study also shows that blastocyst expansion mechanically affects trophectoderm cells, causing nuclear budding and DNA shedding into the cytoplasm. Moreover, the study suggests that aneuploidies in human embryos may not only result from chromosome segregation errors during mitosis, but also from nuclear DNA shedding.
Review
Physiology
Inmaculada Moreno, Antonio Capalbo, Aymara Mas, Tamara Garrido-Gomez, Beatriz Roson, Maurizio Poli, Eva Dimitriadis, Xavier Santamaria, Felipe Vilella, Carlos Simon
Summary: Pregnancy is established during the periconceptional period from blastocyst attachment to placenta formation, significantly impacting the health of both the child and the mother. Advances in understanding the periconceptional space, including the preimplantation human embryo and maternal endometrium, offer opportunities for preventative interventions. This review discusses the role of maternal decidua, the maternal-embryonic interface, the endometrial microbiome, and the myometrium in pregnancy health during the periconceptional period.
PHYSIOLOGICAL REVIEWS
(2023)