Article
Cell Biology
Yu-Chuen Huang, Chun-Ping Huang, Chin-Ping Lin, Kai-Chien Yang, Yu-Jie Lei, Hao-Pei Wang, Yueh-Hsiung Kuo, Yu-Jen Chen
Summary: Daphnoretin extracted from the stem and roots of Wikstroemia indica has been found to inhibit cell growth and induce megakaryocytic differentiation in chronic myeloid leukemia cells. It shows potential as a differentiation therapy agent for CML, but further investigations are needed to validate its efficacy in vivo and in patients.
Article
Medicine, Research & Experimental
Yu Liu, Yufei Chen, Yajun Liu, Mengya Li, Yu Zhang, Luyao Shi, Lu Yang, Tao Li, Yafei Li, Zhongxing Jiang, Yanfang Liu, Chong Wang, Shujuan Wang
Summary: The study found that SMIM3 is highly expressed in adult AML and is associated with poor prognosis. Silencing of SMIM3 can inhibit the proliferation of AML through regulation of the PI3K-AKT signaling pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Mengya Li, Yu Liu, Yajun Liu, Lu Yang, Yan Xu, Weiqiong Wang, Zhongxing Jiang, Yanfang Liu, Shujuan Wang, Chong Wang
Summary: The study found that GNA15 was highly expressed in adult AML, and high GNA15 expression was associated with lower overall survival and relapse-free survival in adult AML with normal karyotype. The inhibition of cell proliferation mediated by GNA15 knockdown may be regulated through the P38 MAPK signaling pathway, suggesting GNA15 as a potential prognostic marker and therapeutic target for AML in the future.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Courtney Chambers, Katerina Cermakova, Yuen San Chan, Kristen Kurtz, Katharina Wohlan, Andrew Henry Lewis, Christiana Wang, Anh Pham, Milan Dejmek, Michal Sala, Mario Loeza Cabrera, Rogelio Aguilar, Radim Nencka, H. Daniel Lacorazza, Rachel E. Rau, H. Courtney Hodges
Summary: In acute myeloid leukemia (AML), SWI/SNF chromatin remodeling complexes sustain leukemic identity by driving high levels of MYC. PU.1 binds to most of its targets in a SWI/SNF-independent manner and recruits SWI/SNF to promote accessibility for other AML core regulatory factors. SWI/SNF inhibition induces therapeutic response in AML but also impairs PU.1-dependent B-cell and monocyte populations, revealing the on-and off-tumor effects of SWI/SNF blockade.
Article
Biotechnology & Applied Microbiology
Yao Liu, Xi Chen, Jingyang Liu, Yinglan Jin, Wei Wang
Summary: The down-regulation of circ_0004277 and up-regulation of miR-134-5p are correlated with the development of AML. Additionally, circ_0004277 inhibits AML development by adsorbing miR-134-5p and up-regulating SSBP2, thereby affecting the proliferation, migration, and invasion abilities of AML cells.
Article
Oncology
Xiao-Yu Chen, Xiao-Hua Qin, Xiao-Ling Xie, Cai-Xiang Liao, Dong-Ting Liu, Guo-Wei Li
Summary: In this study, miR-520a-3p was found to inhibit cell proliferation and promote apoptosis in AML, potentially through the regulation of MUC1 expression and the repression of the Wnt/beta-catenin pathway activation.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Oncology
Zhen Liu, Wenlong Zheng, Yuan Liu, Binghe Zhou, Yuqing Zhang, Fan Wang
Summary: The study showed that HSPA8 is overexpressed in imatinib-resistant CML cells and its ablation can inhibit cell proliferation, induce autophagy, and enhance the anti-tumor activity of imatinib. These findings reveal the role of HSPA8 in IR-CML and suggest its potential as a target for treatment.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Geoffrey Brown
Summary: The origin cell of chronic myeloid leukemia is a hematopoietic stem cell and the hallmark oncogene is BCR-ABLp210, which influences hematopoietic stem and progenitor cells to myeloid fate. Studies have shown that BCR-ABLp210 affects the epigenome, leading to dysregulation of fate choice for hematopoietic stem cells, but the reason why neutrophils are abundantly produced in the disease remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Hongxia Ma, Yang Liu, Zhen Miao, Shijia Cheng, Yunan Zhu, Yifan Wu, Xinxin Fan, Jing Yang, Xingang Li, Liyin Guo
Summary: Neratinib is able to suppress the progression of AML by promoting cell ferroptosis and autophagy, inhibiting cell proliferation, and promoting apoptosis.
DRUG DEVELOPMENT RESEARCH
(2022)
Article
Cell Biology
Ying Wang, Xiaonan Guo, Lihua Wang, Lina Xing, Xiaolei Zhang, Jinhai Ren
Summary: This study found that the expression of miR-342-3p was downregulated and SOX12 was upregulated in acute myeloid leukemia (AML) patients. miR-342-3p affects the apoptosis and proliferation ability of AML cells by regulating the SOX12 gene.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Oncology
Ana Catarina Menezes, Rachel Jones, Alina Shrestha, Rachael Nicholson, Adam Leckenby, Aleksandra Azevedo, Sara Davies, Sarah Baker, Amanda F. Gilkes, Richard L. Darley, Alex Tonks
Summary: The role of RUNX3 in normal myeloid development and leukemia, particularly acute myeloid leukemia (AML), is investigated. The study shows that increased expression of RUNX3 in AML can contribute to the developmental arrest characteristic of the disease. Overexpression of RUNX3 inhibits myeloid differentiation and proliferation in human hematopoietic stem and progenitor cells (HSPC), while RUNX3 knockdown does not impact myeloid growth and development. These findings suggest that dysregulation of RUNX3 may play an important role in the pathogenesis of AML.
Article
Multidisciplinary Sciences
Tao Sun, Lin Dong, Yan Guo, Hai Zhao, Manzhi Wang
Summary: This study revealed key lncRNAs in CN-AML and demonstrated their important roles in the development of AML.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Stefania Trino, Ilaria Laurenzana, Daniela Lamorte, Giovanni Calice, Angelo De Stradis, Michele Santodirocco, Alessandro Sgambato, Antonella Caivano, Luciana De Luca
Summary: This study reveals that AML cells disrupt the hematopoietic process of HSPCs by releasing EVs, which alter gene expression and affect the phenotype and function of HSPCs. This communication may contribute to the formation of a leukemic niche favorable for leukemia development.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Alhomidi Almotiri, Hamed Alzahrani, Juan Bautista Menendez-Gonzalez, Ali Abdelfattah, Badi Alotaibi, Lubaid Saleh, Adelle Greene, Mia Georgiou, Alex Gibbs, Amani Alsayari, Sarab Taha, Leigh-anne Thomas, Dhruv Shah, Sarah Edkins, Peter Giles, Marc P. Stemmler, Simone Brabletz, Thomas Brabletz, Ashleigh S. Boyd, Florian A. Siebzehnrubl, Neil P. Rodrigues
Summary: The study suggests that loss of Zeb1 in adult stem cells leads to significant defects in stem cell renewal and differentiation, which are associated with leukemia signaling. Therefore, Zeb1 plays a crucial role as a transcriptional regulator in hematopoiesis, coordinating HSC self-renewal and differentiation to suppress the potential development of AML.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
You Jiang, Shui-Yan Wu, Yan-Ling Chen, Zi-Mu Zhang, Yan-Fang Tao, Yi Xie, Xin-Mei Liao, Xiao-Lu Li, Gen Li, Di Wu, Hai-Rong Wang, Ran Zuo, Hai-Bo Cao, Jing-Jing Pan, Juan-Juan Yu, Si-Qi Jia, Zheng Zhang, Xin-Ran Chu, Yong-Ping Zhang, Chen-xi Feng, Jian-Wei Wang, Shao-Yan Hu, Zhi-Heng Li, Jian Pan, Fang Fang, Jun Lu
Summary: This study systematically explored the molecular characteristics of CEBPG in AML and identified CEBPG as a potential therapeutic target for AML patients. It was found that CEBPG promotes AML cell proliferation by activating EIF4EBP1, thus contributing to the progression of AML.
CANCER CELL INTERNATIONAL
(2021)
Article
Hematology
Tongjie Wang, Chengxiang Xia, Qitong Weng, Kaitao Wang, Yong Dong, Sha Hao, Fang Dong, Xiaofei Liu, Lijuan Liu, Yang Geng, Yuxian Guan, Juan Du, Tao Cheng, Hui Cheng, Jinyong Wang
Summary: The study reveals a new role for the Nupr1 gene in regulating the quiescence of hematopoietic stem cells (HSC), which can have implications for improving HSC transplantation efficacy. The deletion of the Nupr1 gene activates dormant HSC and provides a competitive advantage in transplantation without compromising their stemness or differentiation capacity. In addition, the inhibition of Nupr1 affects HSC proliferation and engraftment. This finding could contribute to enhancing the success of HSC transplantation.
Article
Hematology
Caiying Zhu, Yu Lian, Chenchen Wang, Peng Wu, Xuan Li, Yan Gao, Sibin Fan, Lanlan Ai, Liwei Fang, Hong Pan, Tao Cheng, Jun Shi, Ping Zhu
Summary: Single-cell transcriptome analysis of residual HSPCs in patients with aplastic anemia revealed lineage-specific alterations in gene expression and transcriptional regulatory networks, indicating a selective disruption of distinct lineage-committed progenitor pools. These findings provide insights into the molecular interactions between engaged T cells and residual HSPCs, potentially offering novel therapeutic opportunities for AA.
Article
Hematology
Dan Guo, Yangyang Zhao, Nan Wang, Na You, Wenqi Zhu, Peiwen Zhang, Qian Ren, Jing Yin, Tao Cheng, Xiaotong Ma
Summary: GADD45g is identified as a novel tumor suppressor in AML, with its reduced expression associated with poor prognosis in patients. Upregulation of GADD45g impairs DNA repair, induces apoptosis, differentiation, and growth arrest in AML cells, and increases sensitivity to chemotherapeutic drugs.
Article
Hematology
Linping Hu, Xiuxiu Yin, Yawen Zhang, Aiming Pang, Xiaowei Xie, Shangda Yang, Caiying Zhu, Yapu Li, Biao Zhang, Yaojin Huang, Yunhong Tian, Mei Wang, Wenbin Cao, Shulian Chen, Yawei Zheng, Shihui Ma, Fang Dong, Sha Hao, Sizhou Feng, Yongxin Ru, Hui Cheng, Erlie Jiang, Tao Cheng
Summary: This study demonstrates that radiation-induced bystander effects impair the long-term hematopoietic reconstitution of human HSCs and affect the colony-forming ability of human hematopoietic progenitor cells. Additionally, the affected human hematopoietic cells show enhanced DNA damage responses and oxidative stress due to bystander effects. Various antioxidants can mitigate these effects, with differing efficacies in vitro and in vivo.
Article
Hematology
Fengjiao Wang, Jiahuan He, Siqi Liu, Ai Gao, Liu Yang, Guohuan Sun, Wanqiu Ding, Chuan-Yun Li, Fanglin Gou, Manman He, Fang Wang, Xiaoshuang Wang, Xiangnan Zhao, Ping Zhu, Sha Hao, Yanni Ma, Hui Cheng, Jia Yu, Tao Cheng
Summary: By analyzing 12 murine adult hematopoietic cell populations, this study identified 30796 editing sites and revealed the dynamic changes of the RNA editome during hematopoietic development. The highly edited Azin1 in HSPCs plays a crucial role in promoting cell differentiation.
Article
Biotechnology & Applied Microbiology
Mei Zhao, Yi-Dan Sun, Mengdi Yin, Juan-Juan Zhao, Si-Ang Li, Guohua Li, Feng Zhang, Jing Xu, Fei-Ying Meng, Beldon Zhang, Xin-Yu Sun, Jian-Ping Zhang, Tao Cheng, Xiao-Bing Zhang
Summary: This study successfully treated hemophilia A in a mouse model using a gene-editing strategy and identified the humoral immune response as the main cause of decreased treatment efficacy. The findings highlight the importance of modulating the innate immune response triggered by liver damage.
HUMAN GENE THERAPY
(2022)
Article
Oncology
Shengnan Yuan, Xiaomin Wang, Shuaibing Hou, Tengxiao Guo, Yanjie Lan, Shuang Yang, Fei Zhao, Juan Gao, Yuxia Wang, Yajing Chu, Jun Shi, Tao Cheng, Weiping Yuan
Summary: The study indicates that patients with co-mutations of JAK3 and PHF6 have shorter survival times, suggesting a potential role of PHF6 in leukemia progression. Phf6 deficiency promotes JAK3(M511I)-induced T-ALL progression by inhibiting the Bai1-Mdm2-P53 signaling pathway independent of the JAK3/STAT5 signaling pathway. Combination therapy with JAK3 and MDM2 inhibitors may potentially increase the drug benefit for T-ALL patients with PHF6 and JAK3 co-mutations.
Review
Biology
Shiru Yuan, Guohuan Sun, Yawen Zhang, Fang Dong, Hui Cheng, Tao Cheng
Summary: The study describes the SMART model developed based on data from HSC studies, aiming to delineate the key characteristics of adult stem cells and speculate on the physiological relevance of stem cells in other tissues. Efforts are now being made to understand ASC biology and develop safe and effective ASC-based therapies.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Hematology
Yinghui Li, Wenshan Zhang, Yu Zhang, Yahui Ding, Ming Yang, Mei He, Xiaolei Liu, Jiali Gu, Shiqi Xu, Zhiwei Feng, Yafang Li, Jingjing Yin, Huier Gao, Henan Song, Hui Xu, Chaoqun Wang, Qing Ji, Shihui Ma, Wanzhu Yang, Weiping Yuan, Xiang-Qun Xie, Tao Cheng, Yingdai Gao
Summary: The use of umbilical cord blood transplant is limited by the number of hematopoietic stem cells. A small molecule inhibitor of p18, 005A, enhances the self-renewal of long-term HSCs in humans, allowing cells to maintain their functionality in secondary recipients.
Editorial Material
Cell & Tissue Engineering
Terry Lappin, Tao Cheng
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Bing Liu, Tao Cheng, Bo O. Zhou, Hui Cheng, Zhaofeng Zheng, Han He, Xinyu Thomas Tang, Han Zhang, Fanglin Gou, Hua Yang, Jiaxuan Cao, Shujuan Shi, Zining Yang, Guohuan Sun, Xiaowei Xie, Yang Zeng, Aiqing Wen, Yu Lan, Jiaxi Zhou
Summary: This study used single-cell RNA sequencing to map the transcriptomic landscape of human fetal bone marrow and spleen hematopoietic stem/progenitor cells (HSPCs). It was found that functional HSCs do not emerge in the bone marrow until 12 post-conception weeks, while they are not detected in the spleen by 14 post-conception weeks.
Article
Medicine, Research & Experimental
Yan Lin, Quan Gu, Shihong Lu, Zengkai Pan, Zining Yang, Yapu Li, Shangda Yang, Yanling Lv, Zhaofeng Zheng, Guohuan Sun, Fanglin Gou, Chang Xu, Xiangnan Zhao, Fengjiao Wang, Chenchen Wang, Shiru Yuan, Xiaobao Xie, Yang Cao, Yue Liu, Weiying Gu, Tao Cheng, Hui Cheng, Xiaoxia Hu
Summary: Severe acute graft-versus-host disease (aGVHD) is a complication of allogeneic hematopoietic stem cell transplantation. The bone marrow (BM) niche is damaged in aGVHD hosts, and the use of the JAK1/2 inhibitor ruxolitinib can restore BM mesenchymal stromal cell (BMSC) function and improve hematopoietic dysfunction caused by aGVHD.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Review
Hematology
Honglin Duan, Tao Cheng, Hui Cheng
Summary: Spatial transcriptomics, through measuring gene activity and mapping it to specific locations, significantly enhances our understanding of biology and disease. This field has rapidly developed, with spatially resolved transcriptomics (SRT) becoming highly multiplexed, high-resolution, and high-throughput due to new technologies. This review summarizes and compares major SRT methods, including imaging-based, sequencing-based, and in situ sequencing methods, and discusses applications in neuroscience, cancer biology, developmental biology, and hematology. Future improvements and potential applications, particularly in adult bone marrow, are also discussed.
Review
Medicine, Research & Experimental
Guohuan Sun, Quan Gu, Junke Zheng, Hui Cheng, Tao Cheng
Summary: Hematopoietic stem cells rely on a regulated microenvironment for normal blood production, but leukemia cells disrupt this environment to promote their own survival, leading to impaired hematopoiesis. Understanding the functions of extracellular vesicles and their potential clinical applications in hematologic malignancies is crucial.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
