Journal
FUNGAL BIOLOGY
Volume 119, Issue 12, Pages 1255-1266Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2015.09.011
Keywords
Cellulase; Enzyme production; Optimal growth temperature; Sordariales; Thermophilic fungi
Categories
Funding
- Dutch Technology Foundation STW, Netherlands Organisation for Scientific Research (NWO) [12468]
- Ministry of Economic Affairs
- EC
- SYNTHESYS
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Thermophilic fungi have the potential to produce industrial-relevant thermostable enzymes, in particular for the degradation of plant biomass. Sordariales is one of the few fungal orders containing several thermophilic taxa, of which many have been associated with the production of thermostable enzymes. The evolutionary affiliation of Sordariales fungi, especially between thermophiles and non-thermophilic relatives, is however poorly understood. Phylogenetic analysis within the current study was based on sequence data, derived from a traditional Sanger and highly multiplexed targeted next generation sequencing approach of 45 isolates. The inferred phylogeny and detailed growth analysis rendered the trait 'thermophily' as polyphyletic within Chaetorniaceae (Sordariales, Sordariornycetes), and characteristic to: Myceliophthora spp., Thielavia terrestris, Chaetomiurn thermophilum, and Mycothermus thermophilus. Compared to mesophiles, the isolates within thermophilic taxa produced enzyme mixtures with the highest thermostability of known cellulase activities. Temperature profiles of the enzyme activities correlated strongly with the optimal growth temperatures of the isolates but not with their phylogenetic relationships. This strong correlation between growth and enzyme characteristics indicated that detailed analysis of growth does give predictive information on enzyme physiology. The variation in growth and enzyme characteristics reveals these fungi as an excellent platform to better understand fungal thermophily and enzyme thermostability. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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