Journal
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 153, Issue 1, Pages 265-272Publisher
SPRINGER
DOI: 10.1007/s10658-018-1525-5
Keywords
Grapevine yellows; Phytoplasma; Planthopper; Bois noir; Epidemiology
Categories
Funding
- bilateral French - Lebanese intergovernmental programme PHC CEDRE 2014-2015 Project [30902ZB]
- INRA metaprogramme Sustainable Management of Crop Health (SMACH) project LYCOVITIS
Ask authors/readers for more resources
Grapevine yellows (GY) affecting Lebanese vineyards are reported to be associated with Candidatus Phytoplasma solani', the bois noir (BN) etiological agent. However, during a field survey in June 2014 for BN in Mansoura municipality of West Bekaa, Lebanon, Candidatus Phytoplasma omanense' was detected in a grapevine sample, cultivar Syrah, exhibiting leaf scorch and discoloration, using a phytoplasma universal nested-PCR and sequencing of the 1.2 kbp 16SrDNA amplicon. The same 1.2 kbp 16SrDNA sequence could be amplified from Hyalesthes obsoletus and Reptalus sp. Cixiidae planthoppers collected on Convolvulus arvensis (bindweed) plants in June 2014 in Aammiq municipality of West Bekaa. Yellowing and stunted bindweed plants collected in 2015 in Kefraya and Aamiq municipalities of West Bekaa were also found infected with the same phytoplasma strain. A 16S rDNA RFLP assay was designed to differentiate this phytoplasma from Ca. P. solani' and Ca. P. phoenicium', which are endemic to the Bekaa region. Ca. P. omanense' is reported for the first time in grapevine and in Cixiidae planthoppers already known to respectively host and vector Ca. P. solani' strains associated with BN disease in grapevine. This result highlights the need for a precise survey of phytoplasmas associated to grapevine yellows in the Eastern Mediterranean basin, and for investigating the possible role of Ca. P. omanense' as a new threat to Euro-Mediterranean viticulture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available