Article
Pharmacology & Pharmacy
Xi Li, Xiaofan Zhang, Heng Cai, Yiwei Zhu, Jingshu Ji, Tingting Qu, Yuexing Tu, Hua Zhou, Yunsong Yu
Summary: Sequence type 235 (ST235) Pseudomonas aeruginosa, associated with high morbidity and mortality, has a relatively high resistance to ceftazidime-avibactam (CZA), partly due to overexpression of blaGES-1 carried in a complex transposon. Overexpression of blaGES-1 combined with an efflux pump results in high-level CZA resistance, reducing therapeutic options for treating infections caused by ST235 P. aeruginosa. Surveillance initiatives are essential to prevent further dissemination of high-risk ST235 P. aeruginosa with CZA resistance.
DRUG RESISTANCE UPDATES
(2023)
Article
Infectious Diseases
Su Young Lee, Christian M. Gill, David P. Nicolau, ERACE PA Global Study Grp
Summary: This study compared the in vitro activity of different drugs against serine beta-lactamase-producing Pseudomonas aeruginosa. Ceftazidime/avibactam demonstrated the best activity and is a rational treatment option.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Yue Fang, Zulqarnain Baloch, Wei Zhang, Ying Hu, Rui Zheng, Yuzhu Song, Wenlin Tai, Xueshan Xia
Summary: This study investigated the prevalence of carbapenem-resistant Pseudomonas aeruginosa in a burn ward in Yunnan province, China and identified different resistance mechanisms. The results highlight the need for enhanced surveillance of carbapenem-resistant strains in burn patients.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Yonggang Zhao, Dingqiang Chen, Kaichao Chen, Miaomiao Xie, Jiubiao Guo, Edward Wai Chi Chan, Lu Xie, Jingbo Wang, Enqi Chen, Sheng Chen, Weijun Chen, Lars Jelsbak
Summary: By sequencing and analyzing the genomes of 416 Pseudomonas aeruginosa strains from hospitals in China, the genetic, phenotypic, and transmission characteristics of carbapenem-resistant strains were elucidated, and the molecular signatures responsible for the increased prevalence of CRPA infections in China were identified. These findings may provide new insight into the development of effective strategies for worldwide control of CRPA and minimize the occurrence of untreatable infections in clinical settings.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ethan R. Wyrsch, Rhys N. Bushell, Marc S. Marenda, Glenn F. Browning, Steven P. Djordjevic
Summary: Lower urinary tract, renal, and bloodstream infections caused by phylogroup B2 extraintestinal pathogenic Escherichia coli (ExPEC) are a leading cause of morbidity and mortality. ST1193 is a multidrug-resistant sequence type of B2 phylogroup E. coli that is associated with various human infections. This study analyzed the virulence plasmids carried by ST1193 and found that they are similar to pUTI89, a key F virulence plasmid found in dominant extraintestinal pathogenic E. coli lineages. The findings of this study have significant implications for the epidemiological source tracking of ExPEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Lidia Ruiz-Roldan, Beatriz Rojo-Bezares, Carmen Lozano, Maria Lopez, Gabriela Chichon, Carmen Torres, Yolanda Saenz
Summary: The study revealed a low occurrence of P. aeruginosa and low levels of antimicrobial resistance in raw vegetables, but highlighted a high number of virulence-related traits in these strains, emphasizing their pathological importance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Mykhailo Savin, Gabriele Bierbaum, Nico T. Mutters, Ricarda Maria Schmithausen, Judith Kreyenschmidt, Isidro Garcia-Menino, Silvia Schmoger, Annemarie Kaesbohrer, Jens Andre Hammerl
Summary: This study characterizes carbapenem-resistant Klebsiella spp. in wastewater and surface water in Germany. The isolates showed resistance to multiple antibiotics and a high diversity of antibiotic-resistance genes. Virulence factors were also present in most isolates. The findings suggest the possible dissemination of resistant bacteria in the environment and the risk of colonization and infection in humans, livestock, and wildlife.
Article
Infectious Diseases
Xiaoxue Liang, Ping Chen, Baoguo Deng, Feng-Hui Sun, Yongqiang Yang, Yanxian Yang, Ruowen He, Mingyang Qin, Yiping Wu, Fan Yang, Guo-Bao Tian, Min Dai
Summary: This study compared the antimicrobial resistance, virulence, clinical characteristics, and risk factors between carbapenem-resistant K. pneumoniae (CRKP) and carbapenem-susceptible K. pneumoniae (CSKP) isolates from patients with bloodstream infections (BSIs) in China. The results demonstrated that CRKP isolates were more drug-resistant, more virulent, and associated with poorer outcomes compared to CSKP isolates.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Chongmei Tian, Jianqin Song, Lingzhi Ren, Delian Huang, Siwei Wang, Liping Fu, Yaping Zhao, Yongfeng Bai, Xueyu Fan, Tianhong Ma, Junjie Ying
Summary: The isolation of a carbapenem-resistant A. johnsonii AYTCM strain from sputum in China in 2018 is reported. The strain co-produced the NDM-1, OXA-58, and PER 1 enzymes, and exhibited resistance to multiple antibiotics. Whole-genome sequencing revealed the presence of 11 plasmids, with bla(OXA-58) and bla(PER-1) genes located on the pAYTCM-1 plasmid. The study highlights the importance of early surveillance to prevent the spread of high-risk clones in healthcare settings. Rating: 7
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Younes Khalili, Pooya Omidnia, Hamid Reza Goli, Sajjad Zamanlou, Farhad Babaie, Abed Zahedi Bialvaei, Aylin Esmailkhani
Summary: This study aimed to analyze the molecular epidemiology and mechanisms of resistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from Tabriz, Iran. The prevalence of CRPA was found to be at alarming levels, with overexpression of efflux systems being the most common mechanism of carbapenem resistance.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Zhengjiang Jin, Zhenhui Wang, Lin Gong, Lu Yi, Nian Liu, Lan Luo, Wenting Gong
Summary: In this study, whole-genome sequencing was conducted on 34 carbapenem-resistant Klebsiella pneumoniae strains. It was found that these strains carried multiple antibiotic resistance genes and virulence genes, and demonstrated resistance to various disinfectants. Variations were observed in the virulence, drug resistance, and disinfectant tolerance among different strains.
Article
Multidisciplinary Sciences
Vijay Kumar, Samantha L. Viviani, Jeeda Ismail, Shreya Agarwal, Robert A. Bonomo, Focco van den Akker
Summary: The study investigated the effects of a novel PBP inhibitor, vaborbactam, on Pseudomonas aeruginosa PBP3, revealing that its binding may enhance protein stability and potentially inhibit penicillin resistance. Vaborbactam forms specific hydrogen bonds, hydrophobic interactions, and covalent bonds with PBP3, suggesting the possibility of developing potent cyclic boronate-based inhibitors to overcome beta-lactamases-mediated resistance.
Article
Biology
Penghao Lv, Guijuan Hao, Yanli Cao, Lulu Cui, Guisheng Wang, Shuhong Sun
Summary: Proteus mirabilis, an opportunistic pathogen, is found to be an emerging threat to both animals and humans for a variety of infections. The study examined the antibiotic resistance genes and virulence genes of P. mirabilis isolates from raccoons, foxes, and minks. The findings revealed high levels of antibiotic resistance and the presence of virulence genes, highlighting the potential environmental threat posed by multidrug-resistant P. mirabilis from animals. The study emphasized the need for further research on the virulence of P. mirabilis.
Article
Microbiology
Jad Atrissi, Annalisa Milan, Raffaela Bressan, Marianna Lucafo, Vincenzo Petix, Marina Busetti, Lucilla Dolzani, Cristina Lagatolla
Summary: This study investigates the complex interplay of multiple factors contributing to carbapenem resistance in Pseudomonas aeruginosa isolates from CF patients, highlighting the role of OprD and OpdP porins, as well as the activity of chromosomal carbapenemases AmpC and PoxB. The analysis of the expression levels of these determinants provides a new approach to understand resistance and susceptibility patterns in clinical isolates, aiding in the selection of appropriate patient-specific therapy.
MICROBIOLOGY SPECTRUM
(2021)
Article
Pharmacology & Pharmacy
Ying Zhou, Chunyang Wu, Bingjie Wang, YanLei Xu, Huilin Zhao, Yinjuan Guo, Xiaocui Wu, Jingyi Yu, Lulin Rao, Xinyi Wang, Fangyou Yu
Summary: This study found that high-virulence K. pneumoniae strains (KL1/KL2) have lower resistance to antibiotics compared to carbapenem-resistant K. pneumoniae strains (CRKP). It also discovered that the pLVPK-like virulence plasmid and IncF blaKPC-2 plasmid are crucial for the formation of hypervirulent and carbapenem-resistant K. pneumoniae.
DRUG RESISTANCE UPDATES
(2023)
Article
Biology
Beatriz Robredo, Rosa Fernandez-Fernandez, Carmen Torres
Summary: This study connects research and education through a service-learning methodology, the Small World Initiative project. It challenges young students to discover novel bioactive-producing microorganisms from environmental soil samples, promoting scientific vocations and communicating scientific culture.
JOURNAL OF BIOLOGICAL EDUCATION
(2023)
Article
Infectious Diseases
Maria Font-Font, Alba Belles-Belles, Rosa Fernandez-Fernandez, Carmen Torres
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Article
Infectious Diseases
Rosa Fernandez-Fernandez, Beatriz Robredo, Enrique Navajas, Carmen Torres
Summary: Antimicrobial resistance is a global problem, and soil is a promising resource for finding new antimicrobial agents. The MicroMundo project, part of the Tiny Earth citizen science project, has successfully raised awareness about antimicrobial resistance and contributed to the search for new alternatives. Through this project, 32 potentially antimicrobial-producing bacteria were isolated from 2600 isolates obtained from soil samples.
Article
Infectious Diseases
Sara Garcia-Vela, Laila Ben Said, Samira Soltani, Ramzi Guerbaa, Rosa Fernandez-Fernandez, Houssem Ben Yahia, Karim Ben Slama, Carmen Torres, Ismail Fliss
Summary: This study aimed to screen and characterize potential inhibitory strains of Enterococcus against necrotic enteritis caused by Clostridium perfringens in poultry. Two potential strains, E. faecium X2893 and X2906, were found to have antimicrobial activity, no acquired resistance genes, no plasmids, and the acm gene implicated in host colonization, making them promising candidates for further research as protective cultures in poultry farming.
Article
Biodiversity Conservation
Idris Nasir Abdullahi, Guillermo Juarez-Fernandez, Ursula Hofle, Teresa Cardona-Cabrera, David Minguez, Javier Pineda-Pampliega, Carmen Lozano, Myriam Zarazaga, Carmen Torres
Summary: Migratory storks could transmit bacteria of public health concern through their colonization, persistence, and excretion. This study aimed to determine the diversity, prevalence, and co-colonization of bacteria in storks' tracheal and nasal samples. The results showed a high prevalence of Staphylococcus and Enterococcus genera, with Staphylococcus sciuri being the most prevalent species. The importance of storks as sentinels and vectors of bacterial transmission is highlighted.
Letter
Microbiology
Saray Mormeneo Bayo, Alba Belles Belles, Ivan Prats Sanchez, Eric Lopez Gonzalez, Jesus Aramburu Arnuelos, Albert Bernet Sanchez, Merce Garcia Gonzalez
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2023)
Article
Infectious Diseases
Saray Mormeneo Bayo, Alba Belles Belles, Diego Vazquez Gomez, Montserrat Planella de Rubinat, Diana Carolina Bayas Pastor, Arturo Morales Portillo, Alfredo Jover Saenz, Eric Lopez Gonzalez, Nuria Prim, Merce Garcia-Gonzalez
Summary: Helicobacter pylori infection is highly prevalent and exhibits alarming levels of resistance globally. Empirical treatment strategies vary based on local clarithromycin resistance rates. This study aimed to determine the antibiotic susceptibility and mechanisms of clarithromycin resistance in our healthcare area. Gastric biopsies were collected and cultured, and antibiotic susceptibility testing was conducted. The most common mutations associated with clarithromycin resistance were detected using multiplex real-time PCR. The study revealed high resistance rates for metronidazole and levofloxacin, a 12.1% clarithromycin resistance rate, and mutations at A2143G and A2142G positions. The findings suggest that therapies containing tetracycline may be a suitable option due to the absence of tetracycline resistance.
Article
Public, Environmental & Occupational Health
Sara Quero, Marina Serras-Pujol, Noemi Parraga-Nino, Carmen Torres, Marian Navarro, Anna Vilamala, Emma Puigoriol, Javier Diez de los Rios, Elisenda Arque, Judit Serra-Pladevall, Alba Romero, Daniel Molina, Roger Paredes, Maria Luisa Pedro-Botet, Esteban Reynaga
Summary: This study investigated the prevalence of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) among workers in the pork production chain. The results showed that the prevalence of MRSA was high among workers in contact with live animals, especially in pig farms, livestock transporters, and slaughterhouses.
Article
Public, Environmental & Occupational Health
Idris Nasir Abdullahi, Carmen Lozano, Carmen Simon, Javier Latorre-Fernandez, Myriam Zarazaga, Carmen Torres
Summary: This study investigated the diversity and carriage rate of nasal Staphylococcus species, as well as the variability of antimicrobial resistance, virulence determinants, immune evasion cluster types, and genetic lineages of Staphylococcus aureus isolates. The study also examined the co-carriage rate of CoNS with S. aureus in the same nasal niche of healthy pigs and pig-farmers. The findings highlight the need for multiple samplings to understand the dynamics of AMR and control the transmission of S. aureus in pig-farms.
Article
Infectious Diseases
Carolina Sabenca, Eliana Costa, Sara Sousa, Lillian Barros, Ana Oliveira, Sonia Ramos, Gilberto Igrejas, Carmen Torres, Patricia Poeta
Summary: The emergence of Klebsiella pneumoniae strains producing ESBL and KPC has become a major public health concern. The ability of these strains to form biofilms is worrisome as it can facilitate the spread of antibiotic resistance and prolong infections in healthcare facilities.
Article
Infectious Diseases
Idris Nasir Abdullahi, Carmen Lozano, Carmen Simon, Myriam Zarazaga, Carmen Torres
Summary: This study characterizes the antimicrobial resistance mechanisms, intra-host species diversity, and intra-species AMR diversity in coagulase-negative staphylococci (CoNS) recovered from the nasal cavities of healthy pigs and pig farmers. The study finds a high level of multidrug resistance, diversity of species within hosts, and diversity of resistance profiles within species, indicating the potential for CoNS to be reservoirs of AMR genes. The study also detects various antimicrobial resistance genes in pigs and pig farmers.
Article
Health Care Sciences & Services
Miquel Angel Sastre-Femenia, Almudena Fernandez-Munoz, Maria Antonia Gomis-Font, Biel Taltavull, Carla Lopez-Causape, Jorge Arca-Suarez, Luis Martinez-Martinez, Rafael Canton, Nieves Larrosa, Jesus Oteo-Iglesias, Laura Zamorano, Antonio Oliver
Summary: A high-resolution analysis of resistance patterns, genomic epidemiology, and resistome of Pseudomonas aeruginosa in Spain over a five-year period reveals a general decrease in resistance but an increase in the proportion of XDR strains producing carbapenemases, with the high-risk clone ST235 becoming more prevalent.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Microbiology
Beatriz Robredo, Rosa Fernandez-Fernandez, Carmen Torres, Ruben Ladrera
Summary: MicroMundo contributes to improving knowledge about antimicrobial resistance and its consequences at all educational levels. Questionnaires and satisfaction surveys were conducted to evaluate the impact of MicroMundo and the optimal age of participants. The results showed significant improvement in knowledge about antimicrobial resistance and increased interest in scientific research.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Meriam B. Said, Lamia Thabet, Sarah Cheriet, Amen A. Messadi, Paula Gomez, Laura Ruiz-Ripa, Senda Sghaier, Bilel Hassen, Abdennaceur Hassen, Carmen Torres, Mohamed S. Abbassi
Summary: The study characterized 25 MRSA clinical isolates collected in a Tunisian hospital, revealing a predominance of the ST239-CC8 lineage and high levels of drug resistance. The findings highlight the urgent need for strict hygiene control measures in the hospital.
LETTERS IN APPLIED MICROBIOLOGY
(2023)