Editorial Material
Hematology
David M. Ross
Summary: Krishnan et al. present a single-cell transcriptomic atlas of chronic myeloid leukemia (CML) at diagnosis in the latest issue of Blood. They identify cellular features at diagnosis that can predict the response to tyrosine kinase inhibitor (TKI) therapy by comparing different groups with varying treatment outcomes.
Editorial Material
Hematology
Kaosheng Lv, Wei Tong
Summary: In this study, Zhao et al found that downregulation of MS4A3 contributes to the persistence, progression, and drug resistance of chronic myeloid leukemia (CML) to the BCR-ABL inhibitor imatinib. Thus, enhancing MS4A3 levels could be a potential therapeutic strategy in treating CML.
Editorial Material
Hematology
Aziz Nazha
Summary: The study suggests that early BCR-ABL1 kinetics can be used to predict the likelihood of treatment-free remission in patients with chronic myeloid leukemia receiving TKIs.
Editorial Material
Hematology
Charles A. Schiffer
Summary: The outlook for patients with chronic phase chronic myelogenous leukemia (CML) has significantly improved in recent years with the development of oral tyrosine kinase inhibitors (TKIs). A study comparing asciminib and bosutinib in chronic phase CML patients previously treated with >= 2 TKIs may offer a better management option for this patient population.
Article
Hematology
David T. Yeung, Naranie Shanmuganathan, Timothy P. Hughes
Summary: Asciminib, a first-in-class allosteric inhibitor of BCR::ABL1 kinase activity, is approved for treating chronic-phase chronic myeloid leukemia patients who failed 2 lines of therapy or have the T315I mutation. It shows high specificity and potency against BCR::ABL1, activity against most kinase domain mutations, and potential for combination therapy. Clinicians now have expanded third-line options, choosing between asciminib and ponatinib in most cases.
Article
Pharmacology & Pharmacy
An-Ni Zhong, Yi Yin, Bing-Jie Tang, Lei Chen, Hong-Wei Shen, Zhi-Ping Tan, Wen-Qun Li, Qun He, Bao Sun, Yan Zhu, Jie Xiao, Zhi-Ping Jiang, Ping Xu
Summary: The study revealed that hsa_circ_0058493 was significantly overexpressed in PBMCs of CML patients and associated with poor clinical efficacy of imatinib. Silencing this circRNA significantly inhibited the development of imatinib-resistant CML cells, suggesting its potential as a therapeutic target.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Alena Malyukova, Dorina Ujvari, Elham Yektaei-Karin, Ana Zovko, Harsha S. Madapura, Marton Keszei, Noemi Nagy, Kourosh Lotfi, Niclas Bjorn, Jonas Wallvik, Minori Tamai, Thao T. T. Nguyen, Koshi Akahane, Takeshi Inukai, Leif Stenke, Daniel Salamon
Summary: Combining TKI treatment with a small-molecule MCL1 inhibitor demonstrated strong synergistic antiviability and proapoptotic effects on CML cells and stem/progenitor cells from untreated CML patients, suggesting that dual targeting of MCL1 and BCR-ABL1 activity may efficiently eradicate residual CML cells without affecting normal hematopoietic stem/progenitors.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Frederic Millot, Meinolf Suttorp, Stephanie Ragot, Guy Leverger, Jean-Hugues Dalle, Caroline Thomas, Nathalie Cheikh, Brigitte Nelken, Marilyne Poiree, Genevieve Plat, Birgitta Versluys, Birgitte Lausen, Marina Borisevich
Summary: The study evaluated the rate of children with childhood chronic myeloid leukemia who remained in molecular response after discontinuing imatinib following sustained deep molecular response (DMR). The findings showed fluctuating molecular free remission rates at different time points post-discontinuation, with no significant influencing factors identified.
Review
Food Science & Technology
Han Sun, Shufang Yang, Weiyang Zhao, Qing Kong, Changliang Zhu, Xiaodan Fu, Fang Zhang, Zhemin Liu, Yuming Zhan, Haijin Mou, Yongjin He
Summary: This review focuses on the strategies to improve fucoxanthin productivity and reduce downstream costs in microalgal production, including strain screening, metabolic engineering, and culture mode design.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Medicine, Research & Experimental
Mansi Shah, Harish Kumar, Shaowei Qiu, Hui Li, Mason Harris, Jianbo He, Ajay Abraham, David K. Crossman, Andrew Paterson, Robert S. Welner, Ravi Bhatia
Summary: In chronic myeloid leukemia (CML), LT-HSCs expressing low c-KIT levels are primitive, quiescent, and drug-resistant leukemia-initiating cells, representing a critical target for eliminating disease persistence.
Article
Oncology
Jan Zuna, Lenka Hovorkova, Justina Krotka, Amelie Koehrmann, Michela Bardini, Lucie Winkowska, Eva Fronkova, Julia Alten, Rolf Koehler, Cornelia Eckert, Lisa Brizzolara, Marie Trkova, Jan Stuchly, Martin Zimmermann, Paola De Lorenzo, Maria Grazia Valsecchi, Valentino Conter, Jan Stary, Martin Schrappe, Andrea Biondi, Jan Trka, Marketa Zaliova, Giovanni Cazzaniga, Gunnar Cario
Summary: In this retrospective analysis, the prognostic relevance of minimal residual disease (MRD) and other features in BCR::ABL1-positive ALL were investigated. The study found that BCR::ABL1-positive ALL can be divided into typical ALL and CML-like subtype. The overall prognosis of typical ALL and CML-like patients was similar, but there were differences in relapse rates and mortality. MRD had a significant prognostic role in typical ALL, while it was not significant in CML-like patients. The study suggests that early distinguishing between typical BCR::ABL1-positive ALL and CML-like patients is important for optimal treatment approaches.
Article
Biotechnology & Applied Microbiology
Xunxun Zhu, Jingru Zhang, Yanping Sun, Yan Wang, Qian Liu, Peng Li, Shuang Yu, Na Liu, Jingjing Ye, Daoxin Ma, Chunyan Ji
Summary: miR-23a acts as a tumor suppressor in chronic myeloid leukemia (CML) by downregulating CRYAB expression to control the sensitivity of CML cells to imatinib.
Article
Oncology
Mario Tiribelli, Roberto Latagliata, Massimo Breccia, Isabella Capodanno, Maria Cristina Miggiano, Francesco Cavazzini, Cristina Bucelli, Immacolata Attolico, Sabrina Leonetti Crescenzi, Sabina Russo, Mario Annunziata, Federica Sora, Massimiliano Bonifacio, Olga Mulas, Giuseppina Loglisci, Alessandro Maggi, Gianni Binotto, Elena Crisa, Anna Rita Scortechini, Anna Paola Leporace, Rosaria Sancetta, Pamela Murgano, Elisabetta Abruzzese, Fabio Stagno, Davide Rapezzi, Debora Luzi, Iolanda Vincelli, Monica Bocchia, Carmen Fava, Alessandra Malato, Monica Crugnola, Michele Pizzuti, Francesca Lunghi, Sara Galimberti, Matteo Dalmazzo, Renato Fanin, Emilia Scalzulli, Robin Foa, Alessandra Iurlo, Giuseppe Saglio, Giorgina Specchia
Summary: This study analyzed the use of frontline TKI therapy in Italian patients with CP-CML and found that 55% of patients chose imatinib as their first-line treatment, while second-generation TKIs were predominantly used in younger patients and those without comorbidities.
Article
Biochemistry & Molecular Biology
Fernanda Malhao, Ana Catarina Macedo, Carla Costa, Eduardo Rocha, Alice Abreu Ramos
Summary: Fucoxanthin has the potential to become an adjuvant for Dox chemotherapy regimens in breast cancer treatment, enhancing its cytotoxic effects and inducing cell death and genotoxic effects. In 3D cell cultures, the combination of Fx and Dox showed higher cytotoxic effects and increased apoptosis compared to isolated compounds.
Article
Oncology
Angela McLigeyo, Jamilla Rajab, Peter Oyiro, Mohammed Ezzi, Yatich Bett, Matilda Ong'ondi, Andrew Odhiambo, Sitna Mwanzi, Nicholas Othieno-Abinya
Summary: This study analyzed the baseline characteristics and factors associated with cytopenia in Chronic Myeloid Leukemia patients treated with imatinib. The results showed no significant differences in gender, age, marital status, occupation, and education level between patients with and without cytopenia. Multivariable analysis revealed that baseline anemia, neutropenia, thrombocytopenia, and thrombocytosis increased the odds of developing cytopenia.
Review
Chemistry, Multidisciplinary
Josefa Domenech, Adriana Rodriguez-Garraus, Adela Lopez de Cerain, Amaya Azqueta, Julia Catalan
Summary: Graphene-based materials (GBMs) are a broad family of carbon-based nanomaterials widely used in nanotechnology. The potential genotoxicity of GBMs has raised concerns in terms of human health. Existing literature suggests that certain GBMs have the potential to induce genetic damage, although the specific damage type depends on the material. However, the variability in GBMs, cellular systems, and research methods used in studies makes it difficult to identify the physico-chemical properties that drive the genotoxicity response to GBMs.
Article
Chemistry, Multidisciplinary
Adriana Rodriguez-Garraus, Amaya Azqueta, Francisco Laborda, Ana C. Gimenez-Ingalaturre, Alba Ezquerra, Luis Lostao, Adela Lopez de Cerain
Summary: The overuse of antibiotics as growth promoters is one of the causes of global antimicrobial resistance. A new material containing silver in a clay base was developed as a feed additive. In vitro genotoxicity evaluation showed that the formulation did not cause gene mutations, chromosomal aberrations, or DNA damage, suggesting its potential as an antimicrobial material in animal feed.
Review
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: This review summarizes the concentrations of 12 synthetic progestins (PGs) in wastewater treatment plants and other matrices. PGs have been found to affect the reproductive fitness and development of biota, particularly in fish. However, research on the environmental concentrations and effects of PGs is still limited.
Article
Food Science & Technology
Maria Alonso-Jauregui, Elena Gonzalez-Penas, Adela Lopez de Cerain, Ariane Vettorazzi
Summary: In this study, an alternative S9 fraction from the kidneys was used to assess the genotoxicity of 12 mycotoxins. The results indicate that the organ used for S9 fraction preparation has an influence on the genotoxic activity of some compounds.
Article
Environmental Sciences
Maria Joao Rocha, Frederico Silva, Eduardo Rocha
Summary: The concentrations of seventeen endocrine disruptor compounds were investigated in the Douro River estuary, showing the widespread presence of these substances. High levels of oestrogens, phytoestrogens, and industrial pollutants were found, indicating ongoing risk of endocrine disruption for the local aquatic wildlife. The physicochemical data suggest direct sewage discharges, which may pose risks to humans through bathing and consumption of local fish.
Article
Environmental Sciences
Fernanda Malhao, Ana Catarina Macedo, Alice Abreu Ramos, Eduardo Rocha
Summary: This study aimed to generate multicellular aggregates using ultra-low attachment plates and characterize them through morphometry, qualitative cytology, and quantitative immunocytochemistry analysis. The different cell lines showed structural differences in their generated MCAs and had varying results in the ICC analysis.
Article
Agriculture, Dairy & Animal Science
Sukanlaya Tantiwisawaruji, Maria J. Rocha, Ana Silva, Miguel A. Pardal, Uthaiwan Kovitvadhi, Eduardo Rocha
Summary: This study conducted a quantitative microscopic examination of the ganglia of adult peppery furrow shell. The results showed that females have a larger glia-to-neuron numerical ratio and greater ganglionic volume compared to undifferentiated adults and males. The research also revealed differences in ganglion types and their total volumes and volume ratios. These findings provide valuable insight into the neurophysiology of bivalves.
Article
Marine & Freshwater Biology
Ines L. Pereira, Celia Lopes, Eduardo Rocha, Tania V. Madureira
Summary: This study optimized a 3D model of brown trout hepatocyte spheroids and used it to investigate the effects of androgens on lipidic target genes. The results suggest that DHT modulates distinct lipidic pathways in brown trout and demonstrate that this 3D model is a valuable tool for physiological and toxicological mechanistic studies.
AQUATIC TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rodrigo F. Alves, Eduardo Rocha, Tania Madureira
Summary: In vitro fish cell cultures are alternative models for in vivo toxicological studies. Two-dimensional cultures have limitations in longevity and maintaining in vivo cellular functions, and three-dimensional systems are proposed to better mimic in vivo effects. Fish hepatocyte spheroids, derived from liver cell lines or primary cultures, are the most used 3D model in fish. They closely resemble in vivo counterparts and have higher metabolic capacity compared to monolayer cultures.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Adriana Rodriguez-Garraus, Maria Alonso-Jauregui, Ana-Gloria Gil, Inigo Navarro-Blasco, Adela Lopez de Cerain, Amaya Azqueta
Summary: A new material composed of kaolin base with silver nanoparticles was developed as an alternative to antibiotics in animal feed. In an in vivo geno-toxicological evaluation in rats, the material showed no adverse effects when administered orally at a dose of 2000 mg/kg b.w. for 28 days. The material did not induce micronuclei or DNA damage in various tissues, and silver content in target organs confirmed the negative results.
Article
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: The distribution patterns and amounts of 56 priority pesticides in the Ave basin were evaluated. The results showed high pesticide concentrations, which have caused significant disturbance to the ecosystem. The pesticide levels exceeded the maximum established by European legislation, posing a risk to aquatic organisms.
MARINE POLLUTION BULLETIN
(2023)
Article
Food Science & Technology
Diana Ansorena, Ruben Ramirez, Adela Lopez de Cerain, Amaya Azqueta, Iciar Astiasaran
Summary: The oxidative stability and genotoxicity of coconut, rapeseed and grape seed oils were evaluated. Samples were subjected to different treatments and changes in peroxide values, thiobarbituric acid reactive substances values, fatty acid profiles, and volatile compounds were analyzed. Mutagenicity was not detected in any case, indicating that the three oils are safe to consume.
Article
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: The Sado basin, an area of intensive agricultural activity, lacks data on water levels of priority pesticides such as fungicides, herbicides, and insecticides. Water samples collected from nine sites along the Sado River Estuary were analyzed, revealing that more than 87% of the pesticides were quantified, with 42% and 72% exceeding the maximum limits established by European Directives 98/83/EC and 2013/39/EU. Fungicides, herbicides, and insecticides had average annual amounts of approximately 3.2 μg/L, 1.0 μg/L, and 12.8 μg/L, respectively. Risk assessment identified invertebrates as the most vulnerable trophic level, implicating chlorpyriphos and cyfluthrin as primary contributors based on acute in vivo assays using Daphnia magna. These findings, coupled with the high phosphate concentrations, indicate environmental and potential human health risks in the Sado waters.
Article
Food Science & Technology
Maria Alonso-Jauregui, Adela Lopez de Cerain, Amaya Azqueta, Adriana Rodriguez-Garraus, Ana Gloria Gil, Elena Gonzalez-Penas, Ariane Vettorazzi
Summary: Mycotoxins, such as AFB1 and STER, are natural food and feed contaminants produced by molds. In a study on Wistar rats, it was found that there was no increase in the percentage of micronuclei in bone marrow when exposed to STER, AFB1, or a mixture of both. However, slight DNA damage was observed in the livers of animals treated with AFB1 or the mixture. LC-MS/MS analysis confirmed exposure to both mycotoxins in plasma, liver, and kidney samples.
Article
Agriculture, Dairy & Animal Science
Rodrigo F. Alves, Celia Lopes, Eduardo Rocha, Tania V. Madureira
Summary: Fish hepatocyte spheroids, a three-dimensional structure with similar morphofunctional properties to the liver, have shown potential as an alternative model for studying fish hepatic adaptive and toxicological responses to xenobiotics. In this study, brown trout hepatocyte spheroids were characterized at different time points up to 25 days in culture, and they exhibited stable morphology and function from the 12th to the 20th day. This model can be used for exposure tests to different xenobiotics, particularly estrogenic compounds.