Article
Biotechnology & Applied Microbiology
Shams T. A. Islam, Jie Zhang, Fabio Tonin, Renske Hinderks, Yanthi N. Deurloo, Vlada B. Urlacher, Peter-Leon Hagedoorn
Summary: Lignin valorization through laccase catalysis can release aromatic products, but different enzymes exhibit significant variations in conversion efficiency and rate. Trametes versicolor laccase follows Michaelis-Menten kinetics in converting Kraft lignin, with specific thermodynamic and kinetic parameters, making it a promising tool for guiding enzymatic lignin valorization strategies.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Physical
Pablo F. Garrido, Margarida Bastos, Adrian Velazquez-Campoy, Alfredo Amigo, Philippe Dumas, Angel Pineiro
Summary: The injection of air into an isothermal titration calorimeter's sample cell allows for accurate determination of the surface tension of the target liquid, despite the periodic contribution from bubbles. The new algorithm successfully identifies and analyzes these contributions, improving the analysis of calorimetric signals.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Robert Kerep, Tino Seba, Valentina Borko, Tin Weitner, Toma Keser, Gordan Lauc, Mario Gabricevic
Summary: Human serum alpha-1 acid glycoprotein is involved in the binding and transport of many drugs. Sialic acid groups play a major role in drug binding. The study found that different sialylation can result in different binding affinities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Iago Dillion Lima Cavalcanti, Francisco Humberto Xavier Junior, Nereide Stela Santos Magalhaes, Mariane Cajuba de Britto Lira Nogueira
Summary: Isothermal titration calorimetry (ITC) is a technique for evaluating the thermodynamic profiles of connection between two molecules, allowing the experimental design of nanoparticles systems with drugs and/or biological molecules. In the field of pharmaceutical nanotechnology, ITC has been increasingly used to understand the interaction mechanisms in the formation of nanoparticles and the behavior of nanocarriers in in vivo studies. This technique provides quick and easy results to optimize the formulation process of nano-systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Tzu-Yi Tai, Fanrui Sha, Xiaoliang Wang, Xingjie Wang, Kaikai Ma, Kent O. Kirlikovali, Shengyi Su, Timur Islamoglu, Satoshi Kato, Omar K. Farha
Summary: This study presents the first systematic investigation on the thermodynamics of protein immobilization in metal-organic frameworks (MOFs). It demonstrates that MOFs provide a hydrophobic stabilizing microenvironment for proteins, leading to an entropically driven encapsulation process. Among a series of Zr-MOFs, pyrene-based NU-1000 exhibits the most favorable encapsulation process for insulin.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Thermodynamics
Ziga Medos, Isidora Cobanov, Marija Bester-Rogac, Bojan Sarac
Summary: This research paper examines the issues associated with power compensation isothermal titration calorimetry, highlighting the possibility of obtaining different enthalpy values with different instruments and setting parameters. It emphasizes that modern calorimeters' dynamic power compensation mode may lead to overcompensation of heat effects, resulting in experimental results heavily reliant on chosen parameters. To avoid these problems, the paper suggests checking raw signals and conducting experiments with low or disabled power compensation when observing switching effects.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Biochemistry & Molecular Biology
Adam Jugl, Miloslav Pekar
Summary: The interactions between hyaluronan and cecropin B were investigated using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. The study found that the molecular weight of hyaluronan played a significant role in the interactions, particularly the transition between different conformations. In water, interactions were saturated at a molar charge ratio of 1.5, possibly due to steric hinderance or functional group interactions. However, in PBS, no interactions were observed, regardless of the molecular weight of hyaluronan, indicating that the interactions were suppressed at higher ionic strength.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Fanrui Sha, Tzu-Yi Tai, Madeleine A. Gaidimas, Florencia A. Son, Omar K. Farha
Summary: Isothermal titration calorimetry (ITC) is a technique for measuring the thermodynamic parameters of binding events, which has applications not only in biological macromolecules but also in synthetic systems like metal-organic frameworks (MOFs). Utilizing ITC in MOFs can provide insights into small molecule adsorption mechanisms and the formation of MOF-based composite materials through non-covalent interactions, with specific experimental considerations discussed. Furthermore, underexplored research directions related to MOFs that could benefit from ITC are highlighted.
Article
Engineering, Environmental
Marawit Tesfa, Jerome F. L. Duval, Remi Marsac, Aline Dia, Jose-Paulo Pinheiro
Summary: Potentiometric acid-base titration curves of humic (nano) particles are influenced by the electrostatics and chemical charges of the particles. This study presents a method for accurately analyzing the titration curves using proton affinity spectra, allowing for a better understanding of the reactivity of the particles towards metals.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Polymer Science
Jacek J. Walkowiak, Rohit Nikam, Matthias Ballauff
Summary: The effective charge of highly charged polyelectrolytes is significantly lowered by a condensation of counterions. This effect is more pronounced for divalent ions. Our study on dendritic polyglycerol sulfate (dPGS) demonstrates that the interactions between dPGS and divalent ions (Mg2+ and Ca2+) show no ion specificity but clear competition between monovalent and divalent ions. These findings support the latest theoretical studies and indicate the sequential replacement of monovalent ions with divalent ions.
Article
Biophysics
Margarida Bastos, Adrian Velazquez-Campoy
Summary: Isothermal titration calorimetry (ITC) is widely utilized in various research areas, and the establishment of a standard operating procedure is crucial for obtaining scientifically accurate and comparable results. The ITC SOP, developed under the efforts of Working Group 4 of the ARBRE-MOBIEU COST Action, emphasizes the importance of good experimental design and machine calibration.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Chemistry, Applied
Wei Liao, Emilie Dumas, Abdelhamid Elaissari, Adem Gharsallaoui
Summary: This study investigated the formation mechanism of multilayer emulsions in oil-in-water systems by using isothermal titration calorimetry (ITC) and dynamic light scattering (DLS) techniques. The results showed that ITC could provide more accurate information about the polymer concentration effect. The titration experiment confirmed that low methoxyl pectin (LMP) mainly bound sodium caseinate (CAS)-coated droplets through electrostatic forces, and stable multilayer emulsions were obtained by titrating CAS-stabilized droplets into LMP.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
Mimosa Sillanpaa, Marica T. T. Engstroem, Petri Taehtinen, Rebecca J. J. Green, Jarmo Kapyla, Anu Nareaho, Maarit Karonen
Summary: Plant tannins have anthelmintic and antiparasitic activities and can be used as complementary nutrients alongside commercial anthelmintics. The interactions between tannins and anthelmintics are limited and can have both synergistic and antagonistic effects depending on the tannin type and method used. Hydrolysable tannins can directly interact with the anthelmintic thiabendazole, and this interaction is strengthened by increasing the number of free galloyl groups and the overall molecular flexibility of tannins.
Article
Biochemistry & Molecular Biology
Eva S. Cobos, Ignacio E. Sanchez, Lucia B. Chemes, Jose C. Martinez, Javier Murciano-Calles
Summary: PDZ domains play a crucial role in cell signaling and cell-cell junctions, being able to recognize various natural targets. The study reveals a basic specificity in each PDZ domain, with host partners mostly being able to bind their corresponding PDZ domain. Additionally, viral ligands show the ability to bind most of the studied PDZ domains, including those not previously described.
Article
Chemistry, Applied
Paola Peluso, David Landy, Lamia Nakhle, Roberto Dallocchio, Alessandro Dessi, Sulaiman Krait, Antonio Salgado, Bezhan Chankvetadze, Gerhard K. E. Scriba
Summary: The complex formation between daclatasvir and gamma-CD or TM-beta-CD was studied using isothermal titration calorimetry and molecular modeling. The results showed that a 2:1 complex was predominantly formed with gamma-CD, while a 1:1 complex was formed to a lesser extent. In contrast, the TM-beta-CD complex had a stoichiometry of 1:1. The complex formation with gamma-CD did not require dissociation of the daclatasvir dimer and had a binding constant of 1.67x10^7 M-2, while the weak TM-beta-CD complex required dissociation of the daclatasvir dimer and had a binding constant of 371 M-1.
CARBOHYDRATE POLYMERS
(2023)
Review
Environmental Sciences
Qingliang Cui, Zhiqin Zhang, Jingzi Beiyuan, Yongxing Cui, Li Chen, Hansong Chen, Linchuan Fang
Summary: This review provides a comprehensive understanding of diverse sources of uranium contamination in soils and their spatial variations globally. The biogeochemical transformation and bioavailability of uranium in soil are greatly influenced by factors including redox potential, pH, metal (hydr)oxides, organic matter, and microorganisms. The toxicity and detoxification mechanisms of uranium in plants and microorganisms have been extensively studied, and further research should focus on molecular mechanisms using omics techniques. The mechanisms and applicability of phytoremediation and microbial bioremediation for uranium-contaminated soils were also summarized.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Environmental Sciences
Jianlong Li, Dan Peng, Zhuozhi Ouyang, Peng Liu, Linchuan Fang, Xuetao Guo
Summary: A large number of plastic products used in agricultural production have low recycling efficiency, resulting in the production of microplastics. This study investigates the microplastic contamination in the main agricultural areas of Xinjiang and finds that the majority of microplastics are bulk-shaped and white, with sizes mostly less than 0.5 mm. The contamination risk index for microplastics in this area is 108.92, indicating a grade III risk level. The study also reveals that there is little difference in microplastic abundance between paddy fields and garden lands, possibly due to similar pollution sources.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Shun Liu, Zhaoyang Liu, Wenfeng Tan, Andrew C. Johnson, Andrew J. Sweetman, Xiaoyan Sun, Yu Liu, Chang Chen, Hao Guo, Hanyu Liu, Xiang Wan, Limei Zhang
Summary: This study investigated the source tracing and transport of PFASs from factories to agricultural fields in a fluorochemical industrial region of China. It also explored the bioaccumulation specificities and prediction of these chemicals in different vegetables, and conducted a comprehensive risk assessment considering precursor degradation. The results showed that PFASs in agricultural soils mainly came from fluorochemical manufacturing and metal processing, and alarming levels of PFASs were found in vegetables. PFASs with short carbon chain or carboxylic acid group exhibited higher transport potentials and bioaccumulation factors, and the number of Casparian strips and protein content in vegetables affected their bioaccumulation factors.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Biodiversity Conservation
Ji Liu, Tianyi Qiu, Josep Penuelas, Jordi Sardans, Wenfeng Tan, Xiaomeng Wei, Yongxing Cui, Qingliang Cui, Chuanfa Wu, Lanfa Liu, Baitao Zhou, Haoran He, Linchuan Fang
Summary: This study found that crop residue inputs can increase soil organic carbon, nitrogen, and phosphorus contents, helping to alleviate soil carbon limitation and maintain balanced soil stoichiometry. The increase in soil organic carbon and carbon-to-nitrogen (phosphorus) ratio reached saturation after around 13-16 years, while the increase in grain yield stopped. A predictive model was proposed to evaluate the potential for crop residue improvement under different soil conditions and nitrogen input levels.
GLOBAL CHANGE BIOLOGY
(2023)
Review
Environmental Sciences
Li Chen, Fayuan Wang, Zhiqin Zhang, Herong Chao, Haoran He, Weifang Hu, Yi Zeng, Chengjiao Duan, Ji Liu, Linchuan Fang
Summary: Soil pollution from potentially toxic elements (PTEs) is a global environmental issue that affects agriculture and human health. Arbuscular mycorrhizal fungi (AMF) can mitigate PTE toxicity in crops, but their comprehensive effects in PTE-contaminated soils have not been globally recognized.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Environmental Sciences
Meng Kou, Jingtao Hou, Chang Chen, Juan Xiong, Renhao Wei, Mingxia Wang, Wenfeng Tan
Summary: This study evaluated the single and combined effects of cadmium (Cd) and lead (Pb) on rice root elongation at various concentrations. The results showed that there was an interaction between Cd and Pb, which weakened their toxicity. Cd had a more significant inhibitory effect on Pb toxicity. The study also introduced a systematic method for assessing heavy metal interaction at different concentration levels, which may help formulate control standards for heavy metal combined pollution in agricultural land.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Ecology
Ji Liu, Linchuan Fang, Tianyi Qiu, Haijian Bing, Yongxing Cui, Jordi Sardans, Enzai Du, Ji Chen, Wenfeng Tan, Manuel Delgado-Baquerizo, Guiyao Zhou, Qingliang Cui, Josep Penuelas
Summary: This study investigated the patterns of plant-microbial N/P limitation in forests across China and found significant divergence between the two. The N/P limitation was disconnected in 42.6% of plant-microbial communities, with only 17.7% of N and 39.7% of P limitations consistent. The divergence was more evident at mid-latitudes, where plants were mainly N limited and microbes were mainly P limited. The findings were consistent with ecological stoichiometry and highlighted the importance of soil chemistry in driving the divergence.
FUNCTIONAL ECOLOGY
(2023)
Article
Environmental Sciences
Liping Jia, Xuelin Cheng, Linchuan Fang, Xuguang Huang
Summary: To enhance the nitrogen removal capacity, this study used scrap iron filings and Si-Al porous clay mineral material to improve a subsurface wastewater infiltration system. The results showed that the improved system had higher removal efficiencies for total nitrogen (TN) and ammonium nitrogen (NH4+-N) compared to the control system. The amending soil matrix enriched nitrogen removal bacteria and improved the activities of key enzymes. It also enhanced phosphate metabolism genes and phosphate-solubilizing microbes, strengthening nitrogen metabolism. Nitrospiraceae, Rhizobiales_Incertae_Sedis, and Gemmatimonadaceae were identified as microbial indicators of nitrogen removal. The environmental factors DO, Fe-2(+), TOC, TP, TN, NH4+-N, and NO3--N were found to affect microbial characteristics. Overall, this study provided a low-cost and efficient enhancement approach for subsurface wastewater infiltration systems.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Review
Engineering, Environmental
Fengyu Huang, Jinzhao Hu, Li Chen, Zhe Wang, Shiyong Sun, Wanming Zhang, Hu Jiang, Ying Luo, Lei Wang, Yi Zeng, Linchuan Fang
Summary: Microplastics (MPs) and cadmium (Cd) have a detrimental impact on plant growth and Cd accumulation in soil-plant systems. MPs negatively affect shoot and root biomass, while promoting Cd uptake. Polyethylene has the strongest effect on Cd accumulation. MPs also lead to an increase in soil available Cd concentration and a decrease in soil pH. Moreover, MPs inhibit photosynthesis and enhance oxidative damage to plants. Overall, this study provides insights into the combined impacts of Cd and MPs on plants and offers guidelines for sustainable use of MPs in agriculture.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Soil Science
Haiyang Liu, Shengnan Zhang, Wei Xu, Hongen Liu, Jiangye Li, Jizheng He, Wenfeng Tan
Summary: The discovery of complete ammonia oxidizers has revolutionized our understanding of two-step nitrification. Previous studies have suggested that these organisms may outcompete canonical ammonia oxidizers under ammonia-limited conditions. However, there is currently no convincing evidence linking their activity to nitrification in acidic soils.
BIOLOGY AND FERTILITY OF SOILS
(2023)
Article
Agronomy
Ji Liu, Linchuan Fang, Tianyi Qiu, Ji Chen, Hai Wang, Muxing Liu, Jun Yi, Hailin Zhang, Cong Wang, Jordi Sardans, Li Chen, Min Huang, Josep Penuelas
Summary: The combined use of organic fertilizers and crop residues can increase soil carbon, nitrogen, and phosphorus stocks, especially in the topsoil. In uplands, crop residue return can mitigate soil acidification and increase grain yield. The increase in soil carbon, nitrogen, and phosphorus stocks also depends on soil conditions and fertilizer levels.
AGRONOMY FOR SUSTAINABLE DEVELOPMENT
(2023)
Article
Biodiversity Conservation
Wenliang Ju, Linchuan Fang, Guoting Shen, Manuel Delgado-Baquerizo, Ji Chen, Guiyao Zhou, Dengke Ma, Haijian Bing, Lei Liu, Ji Liu, Xiaolian Jin, Liang Guo, Wenfeng Tan, Evgenia Blagodatskaya
Summary: This study investigates the long-term effects of grazing exclusion on soil microbial communities and nutrient dynamics within soil aggregates in a semiarid grassland. The results show that long-term grazing exclusion reduces microbial diversity and carbon sequestration potential, while medium-term grazing exclusion promotes carbon sequestration and nutrient cycling.
GLOBAL CHANGE BIOLOGY
(2023)
Meeting Abstract
Horticulture
Yuan Zhou, Pin Yang, Donglin Zhang, Jing Mao, Jun Tong, Dongyun Xu, Yanfang Dong, Linchuan Fang, Lin Xu
