Journal
ELECTROPHORESIS
Volume 39, Issue 11, Pages 1375-1381Publisher
WILEY
DOI: 10.1002/elps.201700470
Keywords
Capillary electrophoresis; Carbon nanotubes; Dual electrode detection; Methyl parathion metabolites; Urine biomarker
Funding
- Research Grants Council of the Hong Kong Special Administrative Region, China [702210]
- Seed Fund in Basic Research from the Hong Kong University Research and Conference Grants Committee
- Natural Science Foundation of Hubei Province [2015CFB412]
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Methyl parathion (MP) is a highly toxic organophosphate and its exposure may lead to substantial adverse effects to human health. The existence of 4-nitrophenol (4-NP) in the form of free phenol, glucuronide (4-NP-G) or as a sulfate ester (4-NP-S) can be used as biomarkers to assess the duration and extent of MP exposure. In this work, a MC-CE device incorporating post-CE amperometric detection using multi-walled carbon nanotubes (MWNTs) modified carbon fiber microelectrode (CFME) was fabricated and assessed for simultaneous determination of 4-NP, 4-NP-G, and 4-NP-S in human urine. The detection sensitivity and stability was greatly enhanced by the modification of MWNTs. The capability of the MC-CE device with dual MWNTs modified CFME for detecting impurity was assessed and reliability established by high recoveries from 95 to 97% for spiked MP biomarkers. The method developed is shown to provide a simple, sensitive, and reliable means for monitoring 4-NP, 4-NP-G, and 4-NP-S in human urine.
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