Journal
ELECTROANALYSIS
Volume 30, Issue 10, Pages 2284-2292Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201800331
Keywords
DNA sensor; electrochemical sensor; electropolymerization; polyaniline; Methylene blue; doxorubicin determination
Categories
Funding
- Russian Science Foundation [17-73-20024]
Ask authors/readers for more resources
New voltammetric DNA sensor has been developed on the base of glassy carbon electrode covered with electropolymerized polyaniline with entrapped native DNA saturated with Methylene blue. The thickness and redox properties of the coating are easily regulated by the number of potential cycles and pH of the solution. Doxorubicin competes with Methylene blue for DNA binding sites and suppresses the electron transfer within the layer. The measurement of the decay of the cathodic peak current made it possible to determine down to 0.01 nM doxorubicin. After that, DNA can be replaced by consecutive treatment of the biosensor with concentrated HCl and fresh DNA solution. Second involvement of DNA was confirmed by electrochemical impedance spectroscopy. The DNA sensor developed was tested on artificial samples mimicking ionic content of human serum and on commercial drug formulation containing doxorubicin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available