Article
Biotechnology & Applied Microbiology
Ricardo M. Chaloub, Victor Hugo S. Monteiro, Mario Giordano
Summary: Low salinities have a negative impact on biomass production, biovolume, and final yield of dry biomass in Isochrysis galbana. However, they do not significantly affect the cellular content of chlorophylls and carotenoids. The content of these pigments increases in the stationary growth phase, regardless of salinity. The total lipid content of the cells is not modified by decreasing salinity. In addition, low salinities reduce the photosynthetic efficiency of the cells, particularly in the stationary growth phase.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yuanjun Xia, Mutsumi Sekine, Minamo Hirahara, Hidemi Kishinami, Fatimah Md. Yusoff, Tatsuki Toda
Summary: Demand for high fucoxanthin and docosahexaenoic acid (DHA) content in Isochrysis galbana is increasing, and it is necessary to develop large-scale cultivation systems with low energy and cost. This study investigated the effects of CO2 concentration and frequency of supply on the productivity and fucoxanthin content of I. galbana. Continuous CO2 supply at 5% concentration showed the highest biomass productivity, while continuous supply at 10% concentration inhibited growth. Intermittent supply of CO2 at 5% and 10% concentrations promoted productivity and fucoxanthin accumulation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Environmental Sciences
Hongli Zheng, Fengru Ge, Kunyan Song, Zixiang Yang, Jinmeng Li, Feng Yan, Xiaodan Wu, Qi Zhang, Yuhuan Liu, Roger Ruan
Summary: Leftover dough hydrolysates can be used to cultivate Isochrysis galbana and produce DHA, utilizing a waste product to produce valuable fish oil. CO2 enrichment significantly affects cell growth and DHA production. Two-stage cultivation strategy can enhance DHA yield.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Oceanography
Zhong Ye, Li Yanrong, Xu Jilin, Cao Jiayi, Zhou Chengxu, Yan Xiaojun
Summary: This study optimized a method for chloroplast isolation from Isochrysis galbana microalgae and determined the purity and integrity of the isolated chloroplasts. The analysis of chloroplast lipids revealed that glycolipids and acylglycerols were the main components.
JOURNAL OF OCEAN UNIVERSITY OF CHINA
(2022)
Article
Multidisciplinary Sciences
Eleonora Colantoni, Francesca Palone, Vincenzo Cesi, Beatrice Leter, Giulia Sugoni, Ilaria Laudadio, Anna Negroni, Roberta Vitali, Laura Stronati
Summary: This study demonstrated for the first time that the microalgae I. galbana is an excellent culture medium for the probiotic L. reuteri. The fermented compound derived from the growth of L. reuteri in I. galbana was shown to significantly reduce the adhesiveness and invasiveness of harmful Escherichia coli. These findings open up new possibilities for the use of microalgae as growth media for probiotics and the production of biofunctional compounds.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Aifeng Li, Jianwei Liu, Jiangbing Qiu, Guixiang Wang, Xianyao Zheng, Ying Ji, Guowang Yan, Peng Zhao, Xizhen Wu, Wenhui Yan, Lei Zhang, Min Li, Yilei Fu
Summary: This study reveals that BMAA can inhibit the growth of marine microalgae, limit the production of ammonia and glutamate through down-regulation of specific genes, and affect gene expression related to photosynthesis and protein synthesis, resulting in cell cycle arrest and accumulation of misfolded proteins.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Lilin Han, Jiangbing Qiu, Aifeng Li, Dongyue Li, Yongmeng Yang, Guixiang Wang, Peiyao Li
Summary: The phycotoxin dinophysistoxins have a wide distribution in marine environments and pose potential threats to marine organisms and human health. This study investigated the effects of dissolved dinophysistoxin-1 (DTX1) on the growth, pigment contents, photosynthetic efficiency, oxidative stress response, and cell cycle of the marine microalga Isochrysis galbana. The results showed that DTX1 significantly inhibited the growth of I. galbana and reduced the photosynthetic efficiency and pigment contents in a dose-dependent manner. DTX1 also increased the levels of reactive oxygen species (ROS) and the contents or activities of oxidative stress-related markers. Cell cycle analysis revealed that DTX1 arrested the cell cycle of I. galbana at the G2/M phase. This study improves our understanding of the ecological effects of DTX1 on marine microalgae and provides fundamental data for establishing water quality criteria for dinophysistoxins in marine organisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Barbara O. Guimaraes, Kieke de Boer, Pieter Gremmen, Anemoon Drinkwaard, Rick Wieggers, Rene H. Wijffels, Maria J. Barbosa, Sarah D'Adamo
Summary: Selenium-enriched ingredients are gaining interest in aquaculture for their positive effects on fish health and growth. This study assessed the suitability of inorganic selenium species for producing selenium-enriched N. oceanica biomass for aquafeed, finding that selenite had minor negative effects on cell growth and was better accumulated than selenate. A concentration of 30μM selenite was selected for pilot-scale cultivation, resulting in similar selenium accumulation compared to flask cultivation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Ceres A. Molina-Cardenas, Marcial L. Lizarraga-Partida, Abraham Guerrero, M. del Pilar Sanchez-Saavedra
Summary: The microalga Isochrysis galbana was found to inhibit the density of pathogenic Vibrio vulnificus strains when co-cultured. The inhibition persisted for at least 12 days after inoculation. These findings suggest that I. galbana cultures can be used in aquaculture settings to reduce the load of V. vulnificus in commercially important organisms.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Chemistry, Medicinal
Xiuyuan Ran, Yuhan Shen, Dongjian Jiang, Chenqi Wang, Xinghui Li, Haoyu Zhang, Yunyun Pan, Chenglin Xie, Tonghui Xie, Yongkui Zhang, Changhong Yao
Summary: This study investigated the production of chrysolaminarin, a water-soluble beta-glucan, by Isochrysis zhangjiangensis microalgae through nutrient deprivation and increased light intensity. The results showed that sulfur deprivation was the most effective strategy for inducing chrysolaminarin accumulation, and higher light intensity further enhanced its production. Chrysolaminarin produced under sulfur deprivation exhibited superior antioxidant activity and has the potential for food and pharmaceutical applications.
Article
Microbiology
Xinfeng Xiao, Wenfang Li, Shuangwei Li, Xingsheng Zuo, Jie Liu, Linke Guo, Xiao Lu, Linlin Zhang
Summary: This study investigates the effects of microplastics on microalgae in the food chain. Polyethylene microplastics have negative impacts on cell growth and protein levels, potentially affecting their use as aquaculture feed. Transcriptome analysis and microbial analysis provide insights into the molecular mechanisms and changes in microbial communities. It emphasizes the importance of reducing microplastic release to protect aquatic ecosystems.
Article
Microbiology
Jia-Yi Cao, Ying-Ying Wang, Min-Nan Wu, Zhou-Yan Kong, Jing-Hao Lin, Ting Ling, Si-Min Xu, Shuo-Nan Ma, Lin Zhang, Cheng-Xu Zhou, Xiao-Jun Yan, Ji-Lin Xu
Summary: The research shows that the marine heterotrophic bacterium Alteromonas macleodii can enhance the growth of Isochrysis galbana, inhibit stress responses, enhance photosynthesis and biosynthesis, and protect the algae from oxidative stress. There may be an exchange of fixed organic carbon and other biomolecules between the autotroph and heterotroph in the co-culture of I. galbana and A. macleodii.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Laura Ballesteros-Redondo, Harry W. Palm, Hanno Bahrs, Alexander Wacker, Adrian A. Bischoff
Summary: The effect of microalgae on the population performance and fatty acid composition of Apocyclops panamensis was studied. The results showed that the group fed with Isochrysis galbana as the main diet performed the best, with higher animal density and proportion of fatty acids.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Ying-Ying Wang, Si-Min Xu, Jia-Yi Cao, Min-Nan Wu, Jing-Hao Lin, Cheng-Xu Zhou, Lin Zhang, Hai-Bo Zhou, Yan-Rong Li, Ji-Lin Xu, Xiao-Jun Yan
Summary: In this study, a growth-promoting bacterial strain Marinobacter sp. was found to significantly enhance the growth and yield of I. galbana, as well as promote the synthesis of DHA. This research provides promising applications for improving productivity and sustainability of aquaculture algal rearing systems.
Article
Genetics & Heredity
Duo Chen, Xue Yuan, Xuehai Zheng, Jingping Fang, Gang Lin, Rongmao Li, Jiannan Chen, Wenjin He, Zhen Huang, Wenfang Fan, Limin Liang, Chentao Lin, Jinmao Zhu, Youqiang Chen, Ting Xue
Summary: This study presents a comprehensive analysis of the regulatory networks for fucoxanthin (Fx) biosynthesis in Isochrysis galbana, a potential bait for functional foods and nutraceuticals. A high-quality genome assembly of I. galbana LG007 was obtained, which revealed its phylogenetic relationship with other Haptophytes. Evolutionary analysis provided insights into the divergence time between I. galbana and Emiliania huxleyi. Gene family and metabolome analyses identified key genes and environmental factors influencing Fx content in I. galbana. These findings contribute to the understanding of Fx biosynthesis and regulation, providing valuable references for food and pharmaceutical applications.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)