Article
Biochemistry & Molecular Biology
Maroun Bou Zerdan, Prashanth Ashok Kumar, Daniel Zaccarini, Jeffrey Ross, Richard Huang, Abirami Sivapiragasam
Summary: Salivary gland carcinomas (SGC) are histologically diverse cancers, and next-generation sequencing (NGS) is used to identify key molecular targets. This study found common mutations and potential predictive markers for immunotherapy in SGC through comprehensive genomic profiling (CGP).
Article
Oncology
Asad Ullah, Jaffar Khan, Abdul Waheed, Nabin Raj Karki, Mya Goodbee, Abdul Qahar Khan Yasinzai, Bisma Tareen, Agha Wali, Khaleel Ahmad Khan, Muhammad Samsoor Zarak, Israr Khan, Andrea Agualimpia Garcia, Adil Khan, Marjan Khan, Sana Jogezai, Junaid Ahmad, Luis Velasquez Zarate, Nikhil Patel, Nagla Abdel Karim, Saleh Heneidi
Summary: Salivary gland tumors are rare and mucoepidermoid carcinoma (MEC) is a common type which mainly affects adults. MEC tumors are usually small in size, moderately differentiated, and localized in the gland. Surgical resection is the most common treatment option. Children with MEC have a lower death rate compared to adults. Higher mortality is associated with larger tumor size, male sex, and distant spread.
Article
Cell Biology
Justin A. Bishop, Lester D. R. Thompson, Bradford Siegele, Jeffrey Gagan, Mena Mansour, Rebecca D. Chernock, Lisa M. Rooper
Summary: Mucoepidermoid carcinoma (MEC) is traditionally defined by a mix of squamoid, intermediate, and mucous cells, but recent studies have found unique cases lacking immunoreactivity for squamous markers despite MAML2 fusions. This study characterizes these tumors and identifies the importance of MAML2 analysis in diagnosing MEC without squamoid cells.
Article
Surgery
Bin Zhou, Zhuo-shan Huang, Wei-liang Chen, Zi-xian Huang, Rui Chen, Kai-fang Yuan, Lei Hong, Yon-ju Chen
Summary: This study investigated the outcomes of patients with mucoepidermoid carcinoma of the palate undergoing pedicled facial-submental artery island flap (FSIF) reconstruction following resection. The results showed that the survival rates of patients with mucoepidermoid carcinoma were closely related to tumor grade and TNM stage.
ASIAN JOURNAL OF SURGERY
(2022)
Article
Cell Biology
Marie-Delphine Lanic, Rene Guerin, Michel Wassef, Pauline Durdilly, Vinciane Rainville, Vincent Sater, Fabrice Jardin, Philippe Ruminy, Valerie Costes-Martineau, Marick Lae
Summary: A new targeted multiplexed next-generation sequencing method has been developed to accurately and rapidly detect gene fusions in salivary gland and sinonasal tumors. The method has been validated in 180 cases and shown to be consistent with conventional techniques, demonstrating its potential application in clinical settings.
Article
Veterinary Sciences
Kayla Alexander, Jennifer Beam, Brittany Baughman, Timothy W. W. Morgan
Summary: Salivary gland neoplasia is rare in cattle, and this study presents a case of high-grade mucoepidermoid carcinoma in a bull. Clinical and postmortem findings supported the diagnosis.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Immunology
Shao-Lin Li, Xi Zhao, Jun-Zhong Tao, Zhen-Zhen Yue, Xiao-Yan Zhao
Summary: mNGS technology is useful for the early diagnosis and clinical antibiotic regimen guidance of infective endocarditis, especially culture-negative cases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Oncology
Lamia Madaci, Laure Farnault, Norman Abbou, Jean Gabert, Geoffroy Venton, Regis Costello
Summary: Cytological approaches have been used for the diagnosis, prognosis, and management of acute myeloid leukemia (AML) and myelodysplastic neoplasms. Technological advances in molecular biology, particularly next-generation sequencing (NGS), have made it possible to quickly identify gene mutations in AML and MDS. The combination of cytological approaches and NGS enables better clinical management and improved prognosis for patients with acute leukemia and myelodysplastic neoplasms.
Article
Immunology
Lei Wang, Na Liu, Lin Zhang, Likun Cui, Mengdie Zhu, Zhifang Li, Panpan Wang, Zhengbin Wang
Summary: This study aimed to evaluate the diagnostic accuracy of metagenomic next-generation sequencing (mNGS) for Klebsiella pneumoniae (Kp) bloodstream infections (BSI). The results showed that mNGS exhibits excellent accuracy for the diagnosis of Kp BSI, providing stronger diagnostic capabilities and optimizing treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Ke Li, Yubao Ma, Rui Ban, Qiang Shi
Summary: This report describes a rare case of alveolar echinococcosis diagnosed using next-generation sequencing. NGS of cerebrospinal fluid accurately detected pathogenic microorganism nucleic acid sequences, providing a new and reliable method for the diagnosis of AE.
FRONTIERS IN GENETICS
(2021)
Article
Immunology
Shilei Wang, Lihua Xing
Summary: Metagenomics next-generation sequencing (mNGS) is a promising method for detecting pathogenic infections, especially Non-Tuberculous Mycobacterial (NTM) and tuberculosis (TB). In this study, mNGS analysis successfully identified six distinct NTM species, primarily Mycobacterium avium complex (MAC). Conventional culture methods faced challenges, resulting in negative results, while qPCR testing showed positive results for the NTM infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Thomas Schmoch, Jens H. Westhoff, Sebastian O. Decker, Annabell Skarabis, Georg F. Hoffmann, Christian Dohna-Schwake, Ursula Felderhoff-Muser, Caroline Skolik, Manuel Feisst, Christina Klose, Thomas Bruckner, Steffen Luntz, Markus A. Weigand, Kai Sohn, Thorsten Brenner
Summary: Sepsis and septic shock have a significant impact on infants and young children, underscoring the need for fast and more sensitive diagnostic methods. The Next GeneSiPS-Trial aims to evaluate the diagnostic performance of NGS technology in critically ill pediatric patients with severe infections.
Article
Medicine, General & Internal
Weiteng Wang, Oudi Chen, Weijiang Liu, Lixi Gan, Xin Li, Qingyan Ma, Xuejiao Hu, Xuhua Jian
Summary: Metagenomic next-generation sequencing successfully identified culture-negative endocarditis caused by Coxiella burnetii and Bartonella. The cardiac valvular tissue samples showed higher suspected pathogen reads than blood specimens. Treatment based on etiology resulted in favorable clinical outcomes, but delayed treatment led to poor prognosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Fang Wang, Shao-Yan Xi, Wen-Wen Hao, Xin-Hua Yang, Ling Deng, Yu-Xia Xu, Xiao-Yan Wu, Liang Zeng, Kai-Hua Guo, Hai-Yun Wang
Summary: This study aimed to investigate the molecular alterations of primary pulmonary salivary gland-type tumors (PSGTs) using targeted next-generation sequencing (NGS). Results showed that MAML2 and MYB rearrangements were detected in a high percentage of MEC and ACC patients. Additionally, ACC patients had mutations enriched in 5 pathways, suggesting potential targeted therapies for improved survival outcomes.
Article
Pathology
Sarah Benton, Jeffrey Zhao, Bin Zhang, Armita Bahrami, Raymond L. Barnhill, Klaus Busam, Lorenzo Cerroni, Martin G. Cook, Arnaud de la Fouchardiere, David E. Elder, Iva Johansson, Gilles Landman, Alexander Lazar, Philip LeBoit, Lori Lowe, Daniela Massi, Lyn M. Duncan, Jane Messina, Daniela Mihic-Probst, Martin C. Mihm, Michael W. Piepkorn, Birgitta Schmidt, Richard A. Scolyer, Christopher R. Shea, Michael T. Tetzlaff, Victor A. Tron, Xiaowei Xu, Iwei Yeh, Sook Jung Yun, Artur Zembowicz, Pedram Gerami
Summary: The study found that additional genomic information can help improve diagnostic agreement and accuracy among expert pathologists when assessing tumors with Spitzoid features, particularly in diagnosing conventional melanoma and classifying lesions into the Spitz category/WHO Pathway.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Hye Kyung Lee, Ludwig Knabl, Ludwig Sr Knabl Sr, Manuel Wieser, Anna Mur, August Zabernigg, Jana Schumacher, Sebastian Kapferer, Norbert Kaiser, Priscilla A. Furth, Lothar Hennighausen
Summary: This study reveals distinct molecular immune responses elicited by SARS-CoV-2 variants carrying the E484K escape mutation throughout the course of COVID-19 disease, and suggests a more persistent retention of immune markers in Alpha+E484K patients.
SCIENTIFIC REPORTS
(2022)
Article
Psychiatry
Xiaogang Zhong, Xin Jin, Li Yan, Lu Yang, Huiqing Long, Jing Wang, Haiyang Wang, Yiyun Liu, Juncai Pu, Peng Xie, Ping Ji
Summary: This study evaluated the reliability and validity of the GHQ-12 in Chinese dental healthcare workers. The results showed that the GHQ-12 had good reliability and validity, and could be used to assess the mental health status of Chinese dental healthcare workers.
FRONTIERS IN PSYCHIATRY
(2022)
Letter
Rheumatology
Ludwig Knabl, Hye Kyung Lee, Mary Walter, Priscilla A. Furth, Lothar Hennighausen
Article
Pathology
Priscilla A. Furth, Weisheng Wang, Keunsoo Kang, Brendan L. Rooney, Grace Keegan, Vinona Muralidaran, Justin Wong, Charles Shearer, Xiaojun Zou, Jodi A. Flaws
Summary: Age is a risk factor for estrogen receptor-positive breast cancer, especially after menopause. The study used genetically engineered mouse models to show that overexpression of estrogen receptor 1 (Esr1) before or during reproductive senescence resulted in a higher prevalence of estrogen receptor-positive adenocarcinomas compared to overexpression of cytochrome P450 family 19 subfamily A member 1 (CYP19A1). The results demonstrate the importance of aging and estrogen pathway-linked signatures in the development of ER-positive mammary adenocarcinomas.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Pathology
Priscilla A. Furth, Weisheng Wang, Keunsoo Kang, Brendan L. Rooney, Grace Keegan, Vinona Muralidaran, Xiaojun Zou, Jodi A. Flaws
Summary: Genetically engineered mouse models with overexpression of Esr1 and CYP19A1 revealed divergent disease processes in mammary glands due to distinct molecular pathophysiological intrusions into estrogen signaling during reproductive senescence. Esr1 overexpression led to higher rates of preneoplasia and cancer compared to CYP19A1 overexpression. Tamoxifen and letrozole exposure down-regulated cell proliferation genes and resolved differences between the two models.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Environmental Sciences
Lu Yang, Li Yan, Xiaogang Zhong, Huiqing Long, Fangchun Chen, Xin Jin
Summary: This study aimed to assess the relationship between job burnout, depressive symptoms, and career choice regret among Chinese dental postgraduates. The results showed that job burnout, depressive symptoms, and career choice regret were prevalent among dental postgraduates and were significantly related to each other.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Virology
Aibing Wang, Dan Zhou, Ewa Krawczyk, Tuanjie Li, Vera Simic, Jie Lu, Xuefeng Liu, Richard Schlegel, Hang Yuan
Summary: Cervical cancer is closely associated with persistent infection of the uterine cervix with high-risk human papillomaviruses (HPV). Activation of the TERT gene and amplification of the TERC gene contribute to the acquisition of the tumorigenic phenotype during the progression of HPV-associated cervical dysplasia to carcinoma. Overexpression of the telomerase holoenzyme in HPV-immortalized cells is sufficient to induce the complete transformed phenotype, including characteristics of epithelial-mesenchymal transition and tumorigenicity.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Kim N. Chi, Neil Fleshner, Vincenzo Emanuele Chiuri, Siska Van Bruwaene, Jason Hafron, Douglas G. McNeel, Peter De Porre, Raymond Scott Maul, Mahesh Daksh, Xiaogang Zhong, Gary E. Mason, Ronald F. Tutrone
Summary: Niraparib, a selective PARP1 and PARP2 inhibitor, has been evaluated in combination therapy for metastatic castration-resistant prostate cancer patients with homologous recombination repair gene alterations. The combination of Niraparib with abiraterone acetate plus prednisone showed promising efficacy and manageable safety profile in this patient population.
Article
Pathology
Xiaoxin X. Wang, Komuraiah Myakala, Andrew E. Libby, Ewa Krawczyk, Julia Panov, Bryce A. Jones, Kanchan Bhasin, Nataliia Shults, Yue Qi, Kristopher W. Krausz, Patricia M. Zerfas, Shogo Takahashi, Parnaz Daneshpajouhnejad, Avi Titievsky, Elizaveta Taranenko, Cyrielle Billon, Arindam Chatterjee, Bahaa Elgendy, John K. Walker, Chris Albanese, Jeffrey B. Kopp, Avi Z. Rosenberg, Frank J. Gonzalez, Udayan Guha, Leonid Brodsky, Thomas P. Burris, Moshe Levi
Summary: This study examined the role of estrogen-related receptors (ERRs) in regulating age-related mitochondrial dysfunction and inflammation in the kidneys. The results showed that treatment with a pan-ERR agonist reversed the age-related kidney dysfunction, while treatment with a STING inhibitor reversed inflammation. These findings highlight potential new drug targets for preventing the progression of age-related kidney disease.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Review
Oncology
Ewa Krawczyk, Joanna Kitlinska
Summary: Neuroblastoma is a pediatric tumor with high heterogeneity, making it challenging to develop preclinical models that accurately reflect its complex biology. This paper discusses the current status of available neuroblastoma models, their strengths and limitations, and future perspectives for preclinical research in this field.
Review
Cell Biology
Danyal Daneshdoust, Mingjue Luo, Zaibo Li, Xiaokui Mo, Sahar Alothman, Bhaskar Kallakury, Richard Schlegel, Junran Zhang, Deliang Guo, Priscilla A. Furth, Xuefeng Liu, Jenny Li
Summary: Preclinical in vitro models, especially conditional reprogramming technology, are crucial in studying breast cancer and have the potential to improve drug discovery and personalized medicine in this field.
Article
Oncology
Fanbiao Kong, Kun Wu, Liming Pang, Yuliang Huang, Lei LI, Jing Xu, Feitong Li, Yan Qing, Zhongyu Wang, Xiurong Huang, Sheng Xu, Xiaogang Zhong, Zhou Zhu, Xiaotong Wang, Jianrong Yang
Summary: The downregulation of Siva-1 was found to enhance the sensitivity of gastric cancer cells to certain chemotherapies by regulating the expression of MDR1 and MRP1 genes through inhibiting the PCBP1/Akt/NF-KB signaling pathway.
Review
Biochemistry & Molecular Biology
Ahmad M. Alamri, Faris A. Alkhilaiwi, Najeeb Ullah Khan
Summary: This review discusses the advancements in molecular diagnostic techniques before and after the emergence of COVID-19, and how the pandemic has accelerated the development of faster, robust, and portable screening devices. The focus of this study is on how the COVID-19 pandemic has expedited the implementation of molecular diagnostic techniques in clinical laboratories to become routine tests.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Meeting Abstract
Urology & Nephrology
Kim Chi, Neil Fleshner, Vincenzo Emanuele Chiuri, Siska Van Bruwaene, Jason Hafron, Douglas G. McNeel, Peter De Porre, R. Scott Maul, Mahesh Daksh, Xiaogang Zhong, Gary E. Mason, Ronald Tutrone
JOURNAL OF UROLOGY
(2022)
Article
Hematology
Abdullah M. Alkahtani, Yasser Alraey, Gaffar Sarwar Zaman, Hajri Al-Shehri, Ibrahim Saeed Alghamdi, Harish C. Chandramoorthy, Ahmed M. Al-Hakami, Ahmad M. Alamri, Hassan Ali Alshehri
Summary: The present investigation compares the clinical attributes and haematological parameters between symptomatic and asymptomatic COVID-19 patients, aiming to identify predisposing signs for COVID-19 related mortality. The study found that blood tests have potential clinical value in predicting COVID-19 progression, and patient characteristics such as age, leukocyte count, RBC, platelets, and differential leukocyte counts may be significant predictors for monitoring the progression of critical illness in SARS-COV-2 patients. These findings are important for redefining treatment procedures and reducing COVID-19 mortalities.
JOURNAL OF BLOOD MEDICINE
(2022)