Article
Hematology
Alisa S. Wolberg, Yaqiu Sang
Summary: This article discusses the pathogenesis of venous thromboembolism and focuses on the role of fibrinogen and factor XIII in thrombus formation and resolution, providing insights for the development of new anticoagulant strategies.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Michal Zabczyk, Joanna Natorska, Anetta Undas
Summary: FXIII is crucial in the modulation of fibrin clot properties in patients with VTE, impacting clot structure and function. FXIII activity is significantly reduced in acute PE and DVT patients, but gradually increases after the acute phase. Reduced FXIII activity is associated with impaired clot permeability and hypofibrinolysis in acute PE patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Sravya Kattula, Yaqiu Sang, Gustaaf de Ridder, Anna C. Silver, Emma G. Bouck, Brian C. Cooley, Alisa S. Wolberg
Summary: The study aimed to develop a mouse model that allows for embolization of RBC- and fibrin-rich VT to determine the role of FXIII in PE risk. By comparing different mouse models, it was found that the IVC stasis model produces thrombi more similar to human thrombi and allows for embolization of existing RBC- and fibrin-rich thrombi. Complete FXIII deficiency increases PE incidence, but partial deficiency does not.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Biochemistry & Molecular Biology
Vassilios P. Memtsas, Deepa R. J. Arachchillage, Diana A. Gorog
Summary: This review article discusses the pathophysiological role and laboratory assessment of fibrin, factor XIII, and endogenous fibrinolysis in the final phase of coagulation cascade and fibrinolysis, as well as their involvement in various disease states and assessment of cardiovascular risk with the most up-to-date evidence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Richard J. Fish, Cristina Freire, Corinne Di Sanza, Marguerite Neerman-Arbez
Summary: Thrombosis assays in larval zebrafish can distinguish between quantitative and qualitative fibrinogen disorder models, providing insights into the thrombotic phenotype of novel fibrinogen mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Zyrina Alura C. Sanchez, Vignesha Vijayananda, Devin M. Virassammy, Liat Rosenfeld, Anand K. Ramasubramanian
Summary: Investigating the motion of cells orthogonal to the direction of main flow is crucial for understanding the development of thrombosis and improving cell separation efficiency.
Review
Hematology
James I. Hearn, Elizabeth E. Gardiner
Summary: This article discusses the role of platelets in the formation of a hemostatic response and vascular injury repair, as well as their function in flowing blood. The article highlights the importance of platelet adhesion and activation in forming and strengthening adhesive ties, and discusses the challenges faced in research.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Hematology
Rameesa D. Syed Mohammed, Francis D. O. Ablan, Nicholas M. McCann, Mohammed M. Hindi, Muriel C. Maurer
Summary: Factor XIII (FXIII) catalyzes the formation of crosslinks between reactive glutamines (Q) and lysines (K). FXIII can be activated proteolytically (FXIII-A*) or nonproteolytically (FXIII-A degrees). The activity and specificity of FXIII-A* and FXIII-A degrees towards different substrates were investigated to gain a better understanding of how FXIII-A targets different substrates.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Rui Vilar, Samuel W. Lukowski, Marco Garieri, Corinne Di Sanza, Marguerite Neerman-Arbez, Richard J. Fish
Summary: The study identified anthralin and all-trans retinoic acid as chemical entities capable of altering fibrinogen production, leading to effects on experimental venous thrombosis. Anthralin may lower fibrinogen levels by affecting the protein secretion pathway.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Environmental Sciences
Jing Zhan, Qian S. Liu, Yuzhu Zhang, Zhendong Sun, Qunfang Zhou, Guibin Jiang
Summary: In this study, the erythrocytotoxicity of silica nanoparticles (SiNPs) on mouse red blood cells (RBCs) was investigated. The results showed that SiNPs induced hemolysis, morphological changes, and phosphatidylserine (PS) exposure in RBCs in a size-related manner. Further investigations revealed that SiNP-60 exposure increased ROS generation, phosphorylation of p38 and ERK1/2, and triggered platelet activation dependent on PS externalization in RBCs and thrombin formation.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Jiali Li, Ze Li, Yaxuan Zhu, Hongyuan Peng, Zehui Du, Shaoguo Ru, Weiwei Wang
Summary: This study investigated the mechanism of red blood cell (RBC)-related diseases induced by Bisphenol S (BPS) using different models. The results showed that oral administration of BPS in rats led to changes in RBC morphology and function, as well as hyperlipidemia and increased RBC membrane cholesterol. In zebrafish embryos, BPS slowed down or even immobilized the flow of RBCs in the tail vein, posing a risk of venous thrombosis. These findings provide new insights into the links between environmental pollutants and venous thrombosis.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Genetics & Heredity
Steven J. Grzegorski, Yakun Zhao, Catherine E. Richter, Chia-Jui Ku, Kari Lavik, Divyani Paul, James H. Morrissey, Jordan A. Shavit
Summary: By using genome editing to generate knockouts of both f3a and f3b in zebrafish, it was found that a single copy of either f3a or f3b is necessary and sufficient for normal lifespan. Complete loss of TF leads to lethal hemorrhage, and TFa and TFb play predominant roles in venous and arterial circulation, respectively. Loss of TF predisposes to stress-induced cardiac tamponade.
Review
Hematology
Amin A. Alamin
Summary: New research is shedding light on the roles played by red blood cells (RBCs) in hemostasis, particularly blood clotting. Previous studies mainly focused on quantitative measures of RBCs, but recent findings are uncovering the various functions of RBCs and their interactions with platelets in clot formation. This renewed interest in RBCs has led to the suggestion that they could be potential targets for treating hemostatic disturbances.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Biology
Helen R. McPherson, Cedric Duval, Stephen R. Baker, Matthew S. Hindle, Lih T. Cheah, Nathan L. Asquith, Marco M. Domingues, Victoria C. Ridger, Simon D. A. Connell, Khalid M. Naseem, Helen Philippou, Ramzi A. Ajjan, Robert A. S. Ariens
Summary: This study investigated the role of subregions in the C-terminus of the fibrinogen alpha-chain in clot structure and stability. Two recombinant fibrinogen variants (alpha 390 and alpha 220) were used to demonstrate differential effects on clot formation and lysis resistance. The findings highlight the critical functions of the alpha C-subregions in fibrin polymerisation and clot stability, suggesting potential implications for novel therapeutics in thrombosis treatment.
Article
Hematology
Deeksha Khialani, Sowmya Vasan, Mary Cushman, Anders Erik Astrup Dahm, Per Morten Sandset, Jacques Rossouw, Astrid van Hylckama Vlieg
Summary: Oral postmenopausal hormone therapy (HT) may lead to venous thrombosis (VT), but testing prothrombotic biomarkers before starting HT may be unnecessary. HT induced prothrombotic changes in biomarkers after one year, but this did not increase the risk of VT.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)