Article
Environmental Sciences
Kejin Chen, Rong He, Li'ao Wang, Lingyue Liu, Xin Huang, Juan Ping, Chuan Huang, Xiang Wang, Yuanyuan Liu
Summary: In shale gas mining areas, indigenous microorganisms can degrade organic pollutants such as petroleum hydrocarbons into CO2 and H2O through aerobic metabolism, but a large quantity of CO2 emissions can exacerbate the greenhouse effect. The experiment found that the removal rate of petroleum hydrocarbons was significantly lower under high-concentration pollution compared to low and medium concentrations, with the largest removal rate in the early stage of culture for each group's TPH concentration. Additionally, the main carbon fixation pathway identified in the study was the reductive citric acid cycle.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Seeu Si Ong, Jia Xu, Choon Kiat Sim, Alexis Jiaying Khng, Peh Joo Ho, Philip Kam Weng Kwan, Aarthi Ravikrishnan, Kiat-Tee Benita Tan, Qing Ting Tan, Ern Yu Tan, Su-Ming Tan, Thomas Choudary Putti, Swee Ho Lim, Ee Ling Serene Tang, Niranjan Nagarajan, Neerja Karnani, Jingmei Li, Mikael Hartman
Summary: This study investigated the microbial flora in patients with idiopathic granulomatous mastitis (IGM) and lactational mastitis (LM). The results showed differences between IGM pus samples and corresponding skin samples, while no significant differences were observed in LM samples. There was no characteristic dominant genus in IGM samples, but Corynebacterium kroppenstedtii showed higher levels in IGM pus samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Xiaoxia Xu, Surajit De Mandal, Hongxin Wu, Shaojie Zhu, Jinrong Kong, Sisi Lin, Fengliang Jin
Summary: The gut microbiota of S. frugiperda is significantly influenced by diet composition, impacting the immune gene expression and ultimately playing a crucial role in pest defense system. The study reveals the differences in gut microbiota and host immunity under different diets, providing important insights for understanding the interaction between insect gut microbiome and diet composition in pest defense.
Article
Immunology
Zhouyu Li, Yongguo Xiang, Yong Wang, Wenjuan Wan, Zi Ye, Shijie Zheng, Yanyi Chen, Liang Xiong, Lu Zhu, Yan Ji, Ke Hu
Summary: This study investigated the composition and function of ocular surface microbiome in healthy individuals from different altitudes. The microbial diversity and richness were significantly lower in highlanders, with distinct differences in bacterial community composition and function compared to lowlanders. The study provides insight into the unique characteristics of ocular microbiome in high altitude regions.
MICROBIAL PATHOGENESIS
(2021)
Article
Oncology
Olfat Khannous-Lleiffe, Jesse R. Willis, Ester Saus, Victor Moreno, Sergi Castellvi-Bel, Toni Gabaldon
Summary: Colorectal cancer (CRC) is a global healthcare challenge influenced by genetic and environmental factors. This study used 16S rRNA sequencing to analyze FIT samples and found that the gut microbiome has predictive potential and undergoes changes during the progression from healthy tissue to carcinoma. These findings have implications for understanding the role of microbes in adenoma to carcinoma progression and improving CRC screening programs.
Article
Medicine, General & Internal
Ramiro Vilchez-Vargas, Jurgita Skieceviciene, Konrad Lehr, Greta Varkalaite, Cosima Thon, Mindaugas Urba, Egidijus Morkunas, Laimutis Kucinskas, Karolina Bauraite, Denny Schanze, Martin Zenker, Peter Malfertheiner, Juozas Kupcinskas, Alexander Link
Summary: This study investigated the gut microbiome composition in twins and found that aging and household sharing were key determinants of microbial similarity and drift.
Article
Neurosciences
Lian Dong, Qian Zheng, Yongran Cheng, Mengyun Zhou, Mingwei Wang, Jianwei Xu, Zucai Xu, Guofeng Wu, Yunli Yu, Lan Ye, Zhanhui Feng
Summary: This study aimed to investigate the intestinal flora of patients with epilepsy and its correlation with epilepsy. Through analysis of the gut microbiota of 71 subjects, significant differences were observed between the epilepsy group and the control group at the genus level. Correlation analysis indicated a significant positive correlation between certain bacterial strains and the occurrence of epilepsy. Functional prediction analysis also revealed significant differences in pathways between the groups. These findings suggest a potential association between the gut microbiota and the development of epilepsy.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Elisabeth Hemberg, Adnan Niazi, Yongzhi Guo, Viktoria J. Debnar, Boglarka Vincze, Jane M. Morrell, Gabriella Kutvolgyi
Summary: Recent studies suggest that there may be a microbial community in the placenta, which is important for the adaptation of foals to life outside the uterus. The study examined the placenta, amniotic fluid, umbilical blood of 24 healthy foaling mares, and jugular blood from foals. Bacterial growth was found in most umbilical blood samples, and bacterial DNA was extracted and sequenced from placental samples, with Proteobacteria and Actinobacteria being the most abundant phyla.
Article
Agriculture, Dairy & Animal Science
Jiaojiao Xu, Jiadi Liang, Wenjun Chen, Xin Wen, Na Zhang, Baohua Ma, Yongde Zou, Jiandui Mi, Yan Wang, Xindi Liao, Yinbao Wu
Summary: In this study, the effect of therapeutic doxycycline on the pig intestinal bacterial community was examined. It was found that doxycycline did not affect the diversity of the microbial community structure, but it did affect bacterial interactions and metabolic pathways. This study provides insight into the impact of doxycycline on the pig intestinal microbiota in pig breeding.
Article
Biochemistry & Molecular Biology
Sergio Candel, Sylwia D. Tyrkalska, Fernando Perez-Sanz, Antonio Moreno-Docon, Angel Esteban, Maria L. Cayuela, Victoriano Mulero
Summary: Perturbations of the microbiome in different body sites can lead to dysbiosis, which is linked to various diseases. The nasopharyngeal microbiome has been found to affect susceptibility to viral infections. Comprehensive studies on the age- and sex-associated changes in the nasopharyngeal microbiome are necessary for understanding the role of the nasopharynx in disease pathogenesis. The microbiome in the nasopharynx of healthy individuals shows stability and resistance to perturbations throughout life, with age- and sex-associated differences observed at the phylum, family, and genus levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Tianyuan Zhang, Hanzhou Li, Silin Ma, Jian Cao, Hao Liao, Qiaoyun Huang, Wenli Chen
Summary: This study evaluated the performance of ONT R10.4.1 16S amplicon and found that it had a lower error rate and better results in synthetic communities. Moreover, the analysis of environmental samples using ONT R10.4.1 showed a similar composition to Pacbio data. Based on these findings, ONT R10.4.1 16S amplicon can also be used for environmental samples.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Ya Wang, Yan Yan, Kelsey N. Thompson, Sena Bae, Emma K. Accorsi, Yancong Zhang, Jiaxian Shen, Hera Vlamakis, Erica M. Hartmann, Curtis Huttenhower
Summary: This study comprehensively evaluates PMA-seq in synthetic and complex microbial communities, finding that the technique is effective for semi-quantitative purposes in simple synthetic communities but provides only qualitative assessments in realistically complex community samples.
Article
Microbiology
Christina Karmisholt Overgaard, Ke Tao, Sha Zhang, Bent Tolstrup Christensen, Zuzana Blahovska, Simona Radutoiu, Simon Kelly, Morten Kam Dahl Dueholm
Summary: Intensive agricultural systems have positive effects on supporting the growing human population, but the excessive use of mineral fertilizers and pesticides may have negative impacts on nutrient cycles and biodiversity. Harnessing the beneficial relationships between plants and plant-associated rhizobacteria can increase nutrient-use efficiency and provide pathogen resistance. Analyzing the plant-associated microbiota using high-throughput sequencing can lead to improved taxonomic classification and identification of new plant-associated microbes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Trina Ekawati Tallei, Fatimawali, Afriza Yelnetty, Diah Kusumawaty, Yunus Effendi, Moon Nyeo Park, Fahad A. Alhumaydhi, Talha Bin Emran, Bonglee Kim
Summary: In this study, the microbial communities and their functions during pineapple peel fermentation were analyzed using a metagenomic approach. It was found that microenvironmental differences have an effect on the microbial composition, and carbohydrate metabolism was the most prevalent metabolic pathway during the fermentation process. All bacteria found in the samples played significant roles in various metabolic processes.
FERMENTATION-BASEL
(2022)
Article
Immunology
Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo
Summary: Accessibility to next-generation sequencing (NGS) has enabled the profiling of microbial communities. Choosing the right 16S rRNA hypervariable region for sequencing is critical. This study evaluated the performance of different hypervariable regions in male urinary microbiota profiling and found that V1V2 region is more suitable.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Ju Han, Fan Wang, Zhihao Li, Lijuan Liu, Ge Zhang, Guoqiang Chen, Jing Liu, Haibo Zhang
Summary: Strain T4 isolated from tobacco waste is capable of resisting toxic inhibitors and producing 2, 3-butanediol under high glucose concentration, showing potential for bioconversion and biochemicals production from biomass waste.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Xiaoqi Zhao, Guoqiang Chen, Fan Wang, Han Zhao, Yuxi Wei, Lijuan Liu, Haibo Zhang
Summary: In this study, a novel exopolysaccharide (EPS) secreted by the extreme salt-tolerant Bacillus subtilis LR-1 was researched. The EPS showed strong antioxidant activity and thickening performance, suggesting its potential application in functional food and related industries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Guoqiang Chen, Zhenxiang Xu, Fan Wang, Lijuan Liu, Yuxi Wei, Jinglong Li, Liang Zhang, Kai Zheng, Lijun Wu, Xiao Men, Haibo Zhang
Summary: The extraction and composition of a new type of microbial polysaccharide FM-EPS were explored in this study, and its antioxidant and antiproliferative activities were investigated. The results indicate that FM-EPS has potential applications.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xuemin Ding, Weixia Ye, Bin Tan, Qiaoyun Song, Yuchan Chen, Wei Liu, Lili Sun, Wei Tang, Yulei Qiao, Qingbo Zhang, Haibo Zhang, Ying Wang, Weimin Zhang, Changsheng Zhang, Wenjun Zhang
Summary: Three new cytochalasins, talachalasins A-C (1-3), were discovered from a deep-sea-derived fungus called Talaromyces muroii sp. SCSIO 40439. The structures and absolute configurations of 1-3 were determined using various analytical techniques. Talachalasins A-C (1-3) are unique cytochalasans with a 168-methyl group, which distinguishes them from over 500 known CYTs. Talachalasin C (3) is a novel 20,21-seco-cytochalasans with a 2-oxabicyclo-[3.3.1]nonan-3-one unit. Talachalasin A (1) exhibited moderate cytotoxic activity against four tumor cell lines (IC50: 3.40-10.02 μM). Talachalasin B (2) demonstrated comparable anti-RSV and HSV-1 activities (IC50: 12.5 p.M or IC50: 20.0 μM).
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Guoqiang Chen, Xin Liang, Xiao Men, Lijuan Liu, Fan Wang, Xichang Bao, Haibo Zhang
Summary: Researchers have developed a novel high-conductivity thin film by spin-coating a layer of polyvinyl alcohol (PVA) on the surface of bacterial cellulose (BC) infused with silver nanowires (AgNWs). This thin film addresses the issues of high AgNWs-AgNWs contact resistance and poor long-term stability, making it suitable for manufacturing a new generation of electronic skin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yajing Yang, Guoqiang Chen, Xiaoqi Zhao, Xiaohe Cao, Lei Wang, Jingjiu Mu, Fenghui Qi, Lijuan Liu, Haibo Zhang
Summary: Exopolysaccharides produced by edible microorganisms, such as S1 and S2, have excellent physicochemical properties and significant biological activity, making them advantageous for the food and pharmaceutical industries. Both S1 and S2 exhibited good thermal stability and potent hydroxyl radical scavenging activity, with S1 also possessing strong iron-reducing capacity. In vitro antitumor assays showed that S1 and S2 significantly inhibited the proliferation of Hela, HepG2, and PC-9 cancer cells, with PC-9 being more sensitive to S1. These findings suggest that S1 and S2 have great potential as natural antioxidants and candidates for cancer treatment in the food and pharmaceutical industries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Bin Tan, Qingbo Zhang, Jieyi Li, Yiguang Zhu, Liping Zhang, Wenjun Zhang, Haibo Zhang, Yang Chen, Changsheng Zhang
Summary: The installation of halogen atoms into aromatic and less activated polyketide substrates by halogenases is a powerful strategy to tune the bioactivity, bioavailability, and reactivity of compounds. In this study, the halogenase TotH was confirmed to catalyze the C-4 chlorination in the biosynthetic pathway of totopotensamide A (1), leading to the formation of ClMeDPG. Six new deschloro totopotensamide (TPM) congeners TPMs H2-H7 (5-10) were isolated and their structures were elucidated. Additionally, the cytotoxicity of compounds 5, 6, 8, and 9 against several cell lines was evaluated.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Agronomy
Chunxiao Yu, Guangmei Wang, Haibo Zhang, Hongpeng Chen, Qian Ma
Summary: The combined application of biochar and nitrification inhibitor DCD on saline-alkali soil in the Yellow River Delta can inhibit nitrification, improve soil properties, and increase soybean yield. The dominant bacteria phylum is Proteobacteria, and bacterial community diversity is significantly influenced by nitrate content and soil aggregates.
Article
Environmental Sciences
Xiaoling Liu, Guangmei Wang, Haibo Zhang, Guangxuan Han, Kexin Li, Andong Wang
Summary: The availability and stoichiometry ratio of nitrogen (N) and phosphorus (P) are important factors in plant trophic dynamics and primary production. However, the response of supratidal wetland herbs to varying N and P supplies is not well understood. In this 4-year field manipulation experiment, the aboveground biomass, leaf N and P concentrations, and N:P ratios of two dominant herbs were examined under different nutrient supply levels and N:P supply ratios. The results showed that the leaf trophic dynamics and aboveground biomass of the herbs were influenced by both overall supply level and N:P supply ratio.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Lijuan Wang, Mengyi Zhu, Qingbo Zhang, Shilan Zhai, Yiguang Zhu, Haibo Zhang, Changsheng Zhang
Summary: In this study, an alternative biological route to produce structurally diverse fidaxomicin aglycones was developed by refactoring the promoter and expressing it in a different host. The resulting titers of the five fidaxomicin aglycones ranged from 4.9 to 15.0 mg L-1. Overall, these findings demonstrate the potential of using a biological strategy to construct and diversify fidaxomicin aglycones.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemical Research Methods
Mengyi Zhu, Lijuan Wang, Haibo Zhang, Liping Zhang, Bin Tan, Qi Huang, Yiguang Zhu, Changsheng Zhang
Summary: Ribosome-targeting oligosaccharides, everninomicins (EVNs), are promising drug leads with a unique mode of action. By duplicating the biosynthetic gene cluster (BGC) in Micromonospora sp. SCSIO 07395, the production of EVNs was enhanced, allowing for bioactivity evaluation. EVNs showed significant inhibition against multidrug-resistant bacteria, with potency comparable or superior to vancomycin, linezolid, and daptomycin. The BGC duplication strategy successfully increased the titer of bioactive EVN M from trace amounts to 98.6 mg L-1, demonstrating the utility of a bioengineering approach for enhanced production and chemical diversification of EVNs.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ge Zhang, Fan Wang, Shuaijun Deng, Guoqiang Chen, Haobao Liu, Haibo Zhang
Summary: This study aimed to obtain a strain capable of producing pectate lyase with good pH stability. Bacillus altitudinis CAS-WZS-08, producing pectate lyase with good pH stability, was isolated. The optimal fermentation conditions for pectate lyase production were determined, and the purified protein was identified as pectate lyase with excellent juice extraction ability.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Hongshuang Liu, Senbiao Fang, Lin Zhao, Xiao Men, Haibo Zhang
Summary: This study identified a novel key site, T182A, in the pentalenene synthase enzyme, which not only affected the solubility and activity of the enzyme, but also influenced the specificity of the product. The study demonstrated the crucial role of the 182 active site in stabilizing and guiding the regioselectivity of carbocations in the reaction.
BIOENGINEERING-BASEL
(2023)
Article
Food Science & Technology
Feng Zeng, Meiying Lai, Quancen Li, Haibo Zhang, Zhixian Chen, Shiyu Gong, Xiaoyan Liu, Bin Liu
Summary: In this study, the antioxidant and anti-aging activities of mannoprotein-rich yeast cell wall enzymatic hydrolysate (MYH) were investigated using the Caenorhabditis elegans (C. elegans) model. The results showed that MYH could increase the lifespan and anti-stress ability of C. elegans by enhancing the activity of antioxidant enzymes and decreasing levels of oxidative stress and apoptosis. Additionally, MYH was found to modulate the expression of specific mRNAs related to antioxidant and anti-aging pathways. Furthermore, MYH was shown to improve the gut microbiota composition and metabolite levels in C. elegans. This study contributes to understanding the antioxidant and anti-aging activities of microorganisms and the development of functional foods.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Computer Science, Information Systems
Xiao Liu, Xingyao Zeng, Chengxiang Hao, Haibo Zhang, Zhongjun Yu, Ting Lv, Meng Li, Zhen Zhang
Summary: In this paper, a novel structure of Ka-band based phased array antenna with calibration function is proposed. The antenna adopts the active phased array system and works in the dual polarization separation mode. The schematic diagram and design details of the Ka-band antenna are provided. The transceiver network and calibration network are discussed, and simulation and measurement results are shown.
CMC-COMPUTERS MATERIALS & CONTINUA
(2023)