Article
Plant Sciences
Piao Yang, Zhenzhen Zhao, Jiangbo Fan, Yinping Liang, Matthew C. C. Bernier, Yu Gao, Lijing Zhao, Stephen Obol Opiyo, Ye Xia
Summary: Our study found that the application of Bacillus proteolyticus strain OSUB18 as a root-drench enhanced the resistance of Arabidopsis plants against Pseudomonas syringae and Botrytis cinerea. This enhancement was achieved through the activation of Induced Systemic Resistance (ISR). We identified several mechanisms by which OSUB18 activates ISR, including enhanced callose deposition, ROS production, and hormone levels such as salicylic acid (SA) and jasmonyl isoleucine (JA-Ile).
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Ranran Wang, Jiaojiao Bai, Guofu Yan, Zejun Xiao, Kexin Chen, Kaikai Li, Jie Tang, Dongping Lu
Summary: This study found that enzymatic hydrolysate of fucoidan (FEH), derived from Sargassum hemiphyllum, can trigger various immune responses in plants, including ROS production, MAPK activation, gene expression reprogramming, callose deposition, stomatal closure, and plant resistance to Pseudomonas syringae pv. tomato (Pst) DC3000. Therefore, FEH has the potential to be used as a non-microbial elicitor to protect plants from pathogen infection in agricultural practices.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Gayani Ekanayake, Reid Gohmann, David Mackey
Summary: The plant apoplast plays a crucial role in photosynthesis and respiration, as it is involved in gas exchange and transpiration. It also serves as a dynamic environment for ion and nutrient transport processes, as well as colonization by various microbes, including pathogens. This study presents a method for isolating and quantifying apoplast fluid in plants, and demonstrates its utility in studying bacterial effectors and disease progression.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Noel Djitro, Rebecca Roach, Rachel Mann, Brendan Rodoni, Cherie Gambley
Summary: This study reports the first identification of Pseudomonas syringae strains infecting zucchini in Australia, causing significant yield losses. The isolates exhibited variations in pathogenicity across different plant species and some produced fluorescent pigment. Additionally, it was discovered that different zucchini varieties showed varying levels of susceptibility to the isolates.
Article
Biochemistry & Molecular Biology
Margot Raffeiner, Suayib Uestuen, Tiziana Guerra, Daniela Spinti, Maria Fitzner, Sophia Sonnewald, Susanne Baldermann, Frederik Boernke
Summary: Xanthomonas effector protein XopS enhances disease susceptibility by repressing defense gene expression and suppressing stomatal immunity through interaction with and inhibition of WRKY40, a transcriptional regulator. XopS manipulation of WRKY40 stability alters downstream gene expression and phytohormone crosstalk to promote pathogen proliferation.
Article
Biochemistry & Molecular Biology
Isabel Orf, Hezi Tenenboim, Nooshin Omranian, Zoran Nikoloski, Alisdair R. Fernie, Jan Lisec, Yariv Brotman, Mariusz A. Bromke
Summary: This study analyzed the differences in resistance to the pathogen Pseudomonas syringae pv. tomato (Pst) between Arabidopsis thaliana accessions Col-0 and C24, and found that the resistance differences mainly involve salicylic-acid-dependent systemic acquired resistance mechanisms and differential activity of certain metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Barbara Emmenegger, Julien Massoni, Christine M. Pestalozzi, Miriam Bortfeld-Miller, Benjamin A. Maier, Julia A. Vorholt
Summary: The authors investigate microbiota properties for plant protection using synthetic communities and machine learning approaches. They identify strains that reduce pathogen colonization despite variation in microbiota composition.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Rizwana Begum Syed Nabi, Nkulu Kabange Rolly, Rupesh Tayade, Murtaza Khan, Muhammad Shahid, Byung-Wook Yun
Summary: The study showed that the atbzip62 mutant line lacking the AtbZIP62 gene exhibited enhanced resistance phenotype against Pst DC3000 vir, suggesting that AtbZIP62 may act as a negative regulator of basal defense and systemic acquired resistance in plants under pathogenic bacterial infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Hyeran Moon, A-Ram Jeong, Oh-Kyu Kwon, Chang-Jin Park
Summary: Orphan genes are commonly found in genomes, but their functions are largely unknown. In this study, a defense-related orphan gene Xio1 was identified and found to play an important role in rice's defense mechanism, providing a new resource for crop breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Valeria Di Paolo, Fiorella Masotti, Cecilia V. Vranych, Carolina Grandellis, Betiana S. Garavaglia, Natalia Gottig, Jorgelina Ottado
Summary: Plant natriuretic peptides (PNPs) play an important role in regulating ions and water homeostasis in plants, and the causal agent of citrus canker disease, Xanthomonas citri subsp. citri (Xcc), possesses a similar peptide called XacPNP. Both AtPNP-A and XacPNP require the presence of the receptor AtPNP-R1 to induce stomatal aperture and maintain photosynthetic efficiency and water homeostasis in plant tissues during infection. The interaction between XacPNP and AtPNP-R1 triggers similar plant responses as its plant counterpart.
Article
Plant Sciences
Karl J. Schreiber, Jana A. Hassan, Jennifer D. Lewis
Summary: The pathogen Pseudomonas syringae effector HopZ1a interacts with the Arabidopsis transcriptional regulator ABR1, which plays a key role in host susceptibility to the pathogen. ABR1 comprises a conserved AP2 domain with DNA-binding activity and structurally disordered regions that enhance DNA binding and transcriptional activation. Loss of ABR1 in mutant plants reduces susceptibility to P. syringae strains, indicating its importance in host-pathogen interactions.
Article
Cell Biology
Hannah M. McMillan, Sophia G. Zebell, Jean B. Ristaino, Xinnian Dong, Meta J. Kuehn
Summary: OMVs from Pseudomonas syringae and P. fluorescens can activate plant immune responses to protect against bacterial and oomycete pathogens. The immunomodulatory activity of OMVs from these species varies in sensitivity to biochemical stressors, reflecting differences in OMV content. Furthermore, plant responses mediated by OMVs are distinct from those triggered by other bacterial epitopes or effector molecules.
Article
Plant Sciences
Xiaomu Zhang, Philip J. Tubergen, Israel D. K. Agorsor, Pramod Khadka, Connor Tembe, Cynthia Denbow, Eva Collakova, Guillaume Pilot, Cristian H. Danna
Summary: Activation of the FLS2 receptor suppresses both early infection stages' virulence and long-term growth of PstDC3000 by promoting glutamine and serine buildup in the leaf apoplast. MAMP perception in Arabidopsis induces the accumulation of free amino acids in a SA-dependent manner. Glutamine and Serine, when co-infiltrated, suppress virulence gene expression and robust infections in susceptible plants.
Article
Plant Sciences
Aboubakr Moradi, Mohamed El-Shetehy, Jordi Gamir, Tina Austerlitz, Paul Dahlin, Krzysztof Wieczorek, Markus Kunzler, Felix Mauch
Summary: The study showed that expressing Coprinopsis cinerea lectin 2 (CCL2) in Arabidopsis enhanced plant resistance against various nematodes and fungal pathogens, while also improving plant growth and biomass production. The mechanism of CCL2-mediated disease resistance enhancement depended on its fucoside-binding ability, and not on direct growth inhibition. Transcriptional induction of defense genes was significantly increased in CCL2-expressing plants compared to wild type plants, indicating the potential of fungal defense lectins in plant protection.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Derek Seto, Madiha Khan, D. Patrick Bastedo, Alexandre Martel, Trinh Vo, David Guttman, Rajagopal Subramaniam, Darrell Desveaux
Summary: This study identified a small molecule called Zaractin that mimics the immune eliciting activity of the Pseudomonas syringae effector HopF1r, showing that both HopF1r and Zaractin activate the same NLR-mediated immune pathway in Arabidopsis. The findings suggest that the ETI-inducing action of pathogenic effectors can be used to identify synthetic activators of the eukaryotic immune system.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Katia Bonaldi, Zheng Li, S. Earl Kang, Ghislain Breton, Jose L. Pruneda-Paz
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Zixing Li, Zheng Li, Xiang Gao, Viswanathan Chinnusamy, Ray Bressan, Zhi-Xin Wang, Jian-Kang Zhu, Jia-Wei Wu, Dong Liu
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Liangsheng Wang, Shan Lu, Ye Zhang, Zheng Li, Xiaoqiu Du, Dong Liu
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2014)
Article
Plant Sciences
Liangsheng Wang, Zheng Li, Weiqiang Qian, Wanli Guo, Xiang Gao, Lingling Huang, Han Wang, Huifen Zhu, Jia-Wei Wu, Daowen Wang, Dong Liu
Article
Plant Sciences
Ming Wang, Zhenhui Zhong, Javier Gallego-Bartolome, Zheng Li, Suhua Feng, Hsuan Yu Kuo, Ryan L. Kan, Hoiyan Lam, John Curtis Richey, Linli Tang, Jessica Zhou, Mukun Liu, Yasaman Jami-Alahmadi, James Wohlschlegel, Steven E. Jacobsen
Summary: It was found that besides DNA methylation, there are many other pathways that can be used to regulate gene expression, and a machine learning model can accurately predict the efficacy of each pathway. In addition, it was also discovered that some proteins can target gene silencing in the dCas9-SunTag system, providing more tools for targeted gene manipulation.