Review
Obstetrics & Gynecology
Marisa Kometas, Gregory M. Christman, Joseph Kramer, Alice Rhoton-Vlasak
Summary: The effectiveness of vitrification compared to slow freezing for preserving ovarian tissue remains controversial, with limited clinical data and inconsistent findings from biochemical studies. While early clinical outcomes suggest favorability towards vitrification, further evaluation is needed to determine its long-term efficacy. Despite potential lack of difference in outcomes between the two methods, the cost-effectiveness and efficiency of vitrification make it a more accessible option.
REPRODUCTIVE SCIENCES
(2021)
Article
Obstetrics & Gynecology
Yodo Sugishita, Lingbo Meng, Yuki Suzuki-Takahashi, Sandy Nishimura, Sayako Furuyama, Atsushi Uekawa, Akiko Tozawa-Ono, Junko Migitaka-Igarashi, Tomoe Koizumi, Hibiki Seino, Yasunori Natsuki, Manabu Kubota, Junki Koike, Keisuke Edashige, Nao Suzuki
Summary: After thawing/warming, at least 60 minutes of diffusion washing in media was necessary to significantly reduce the residual cryoprotectants in ovarian tissues frozen by slow freezing or vitrification.
HUMAN REPRODUCTION
(2022)
Article
Obstetrics & Gynecology
L. Ramos, S. Galbinski, A. Nacul, M. F. Jimenez, N. Frantz, A. Bos-Mikich
Summary: The study compared the effects of slow freezing/thawing and vitrification/warming for cryopreservation of ovarian tissue, finding significant damage in primordial and primary follicles.
REPRODUCTIVE SCIENCES
(2022)
Review
Genetics & Heredity
Supriya Behl, Vidhu B. Joshi, Nicholas B. Larson, Maia C. Young, Muhammad Bilal, David L. Walker, Zaraq Khan, Candace F. Granberg, Asma Chattha, Yulian Zhao
Summary: This systematic review and meta-analysis compared vitrification and slow freezing, the two main methods of ovarian tissue cryopreservation. The analysis of 19 studies showed a significantly higher proportion of intact stromal cells in vitrified tissue compared to slow-frozen tissue. However, no significant differences were observed between the two methods in terms of intact primordial follicles, DNA fragmentation, or primordial follicle density. Despite this, due to differences in stromal cell viability, vitrification may be the preferred option for preserving tissue histology.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Genetics & Heredity
Yodo Sugishita, Enes Taylan, Tai Kawahara, Bunyad Shahmurzada, Nao Suzuki, Kutluk Oktay
Summary: The study revealed that slow freezing and vitrification with open or closed devices showed no significant differences in the survival of primordial follicles, DNA damage, and apoptosis rates in ovarian tissues after cryopreservation and thawing/warming.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Multidisciplinary Sciences
Takeru Ishizaki, Yasuto Takeuchi, Kojiro Ishibashi, Noriko Gotoh, Eishu Hirata, Kosuke Kuroda
Summary: Cryopreservation of tissues is a difficult challenge. Vitrification has recently emerged as a suitable method for tissue cryopreservation, while slow-freezing also shows potential. In this study, an aqueous solution containing a novel cryoprotectant, combined with DMSO, improved the cryopreservation of cell spheroids compared to a commercial cryoprotectant. The optimized solution composition was 10 wt% zwitterion, 15 wt% DMSO, and 75 wt% water.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Andreas Schallmoser, Rebekka Einenkel, Cara Faerber, Norah Emrich, Julia John, Nicole Saenger
Summary: A high-throughput protocol for vitrification of human ovarian tissue suitable for clinical processing has been developed and evaluated. The follicular viability of vitrified samples showed no significant differences compared to slow frozen samples, indicating high proportions of follicular survival rates with both methods. This suggests that high-throughput vitrification is a promising option in a clinical setting.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Genetics & Heredity
Aina Borras, Dolors Manau, Francesc Fabregues, Sara Peralta, Josep Maria Calafell, Gemma Casals, Adela Saco, Ines Agusti, Francisco Carmona
Summary: The study compares the effectiveness of VT and SF techniques in ovarian tissue from AFAB transgender people. Results show that VT preserves follicle and stromal tissue better than SF, but both techniques exhibit minor damage compared to fresh tissue. In conclusion, both cryopreservation methods are effective in maintaining follicular population and stromal tissue.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Biology
Mahboubeh Vatanparast, Mojgan Karimizarchi, Iman Halvaei, Maria Grazia Palmerini, Guido Macchiarelli, Mohammad Ali Khalili
Summary: This study analyzed the structure and ultrastructure of human ovarian tissue after cryopreservation and transplantation onto chick embryo chorioallantois membrane (CAM). The results showed that freezing methods could affect the quantity and quality of follicles, but did not result in malignant cell contamination. These findings are important for improving the preservation and lifespan of ovarian tissue.
Article
Reproductive Biology
C. M. Picazo, C. Castano, P. Boveda, A. Toledano-Diaz, R. Velazquez, B. Pequeno, M. C. Esteso, J. Gadea, S. Villaverde-Morcillo, J. Cerdeira, J. Santiago-Moreno
Summary: This study compared the effects of slow freezing and vitrification on different cell types in canine and wild boar testicular tissue. The results showed that vitrification had higher cell viability and DNA integrity in dogs, while either method could be used in wild boars.
Article
Obstetrics & Gynecology
Pauline Jaeger, Cyrielle Fournier, Claire Santamaria, Eloise Fraison, Nicolas Morel-Journel, Mehdi Benchaib, Bruno Salle, Jacqueline Lornage, Elsa Labrune
Summary: This study evaluated the effects of slow freezing and vitrification on human ovarian tissue cryopreservation. The results showed that vitrification had no significant effect on follicular quality and gene expression, while slow freezing altered primordial follicles and gene expression to some extent.
Article
Reproductive Biology
M. Umair, M. Beitsma, M. de Ruijter-Villani, C. Deelen, C. Herrera, T. A. E. Stout, A. Claes
Summary: The cryotolerance of equine blastocysts larger than 300 mu m can be improved by aspirating blastocoele fluid prior to vitrification; however, it is not known whether blastocoele aspiration also enables successful slow-freezing. The aim of this study was therefore to determine whether slow-freezing of expanded equine embryos following blastocoele collapse was more or less damaging than vitrification.
Article
Biology
S. Lierman, A. Bus, S. Andries, E. Trias, P. E. J. Bols, K. Tilleman
Summary: The study aimed to assess the feasibility of passive slow freezing (PSF) using Mr. Frosty container as an alternative to controlled slow rate freezing (CSF) for human ovarian tissue cryopreservation. Results indicated that PSF may be an easy, cost-effective low-risk alternative to CSF for cryopreservation of human OT, showing higher follicle activation after 2 days in vitro and 2 weeks following xenotransplantation in mice.
Article
Endocrinology & Metabolism
Hyun Sun Kong, Yeon Hee Hong, Jaewang Lee, Hye Won Youm, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Summary: The use of antifreeze protein treatment during the warming process has been shown to improve the quality of bovine ovarian tissue after xenotransplantation, with increased morphological follicle normality and decreased apoptotic rates.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell Biology
Amir Arav
Summary: This review discusses the current status and key factors of cryopreservation of large tissues and organs, introducing the principles of directional freezing and vitrification, and summarizing the ways in which cryopreservation affects large tissues and organs.
Article
Agriculture, Dairy & Animal Science
Fernanda Paulini, Eduardo O. Melo
Summary: The study revealed an inhibitory effect of GDF9 and BMP15 on progesterone secretion in bovine granulosa cells cultured in vitro, along with the inhibition of the steroidogenic marker STAR gene expression.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Cell Biology
Michelle Silva Araujo, Otavio Luis de Oliveira Henriques Paulo, Fernanda Paulini, Daniel de Souza Ramos Angrimani, Miriam Harumi Tsunemi, Camila de Paula Freitas Dell'Aqua, Frederico Ozanam Papa, Fabiana Ferreira de Souza
Summary: This study aimed to evaluate the influence of seminal plasma on the quality and oxidative stress of extended canine semen stored at 5 degrees C for 7 days. The results demonstrated that the presence or lack of seminal plasma during cooling the semen of dogs does not influence sperm quality at 5 degrees C.
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Cell & Tissue Engineering
Gustavo Henrique DonaRodrigues Almeida, Rebeca Piatniczka Iglesia, Michelle Silva Araujo, Ana Claudia Oliveira Carreira, Erika Xavier dos Santos, Celso Vitor Alves Queiroz Calomeno, Maria Angelica Miglino
Summary: Tissue engineering is an innovative approach that has shown significant progress in rebuilding the uterus and its tissues in recent years to address reproductive issues. Research focuses on repair strategies, scaffold production, biomaterials, and animal models, contributing to advancements in regenerative medicine.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Pharmacology & Pharmacy
Jose Luiz P. R. Jivago, Juliana Lis Mendes Brito, Gustavo Capistrano, Marcus Vinicius-Araujo, Ediron Lima Verde, Andris Figueiroa Bakuzis, Paulo E. N. Souza, Ricardo Bentes Azevedo, Carolina Madeira Lucci
Summary: The study found that testicular MNH is highly effective as a non-surgical sterilization method for animals, causing progressive seminiferous tubule degeneration followed by substitution of the parenchyma with stromal tissue and gonadal atrophy, suggesting an irreversible process with few side effects to general animal health.
Article
Agriculture, Dairy & Animal Science
Fernanda Paulini, Michelle Silva Araujo, Luciano Paulino Silva, Carolina Madeira Lucci
Summary: This proteomic study investigated the molecular profiles of preantral follicles in bovine ovarian tissue, revealing the differential molecular compositions among various follicular classes. The analysis showed clustering similarities among primary and secondary follicles in certain m/z ranges which were distinct from primordial follicles. The study aims to pave the way for future research in identifying specific proteins involved in follicle activation in bovine follicles, potentially improving mammalian follicle culture systems.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Andrology
Vitoria Frias Batista, Gustavo de Sa Schiavo Matias, Ana Claudia Oliveira Carreira, Lawrence Charles Smith, Rafaela Rodrigues, Michelle Silva Araujo, Dara Rubia Souza Silva, Felipe de Jesus Moraes, Joaquim Mansano Garcia, Maria Angelica Miglino
Summary: This study investigated the potential application of tissue engineering techniques for infertility treatment. Culturing viable spermatozoa in vitro from pluripotent embryonic cells in testicular scaffolds shows promise in solving male infertility. By culturing embryoid bodies in decellularized rat testis scaffolds, the differentiation of embryonic stem cells into male germ cells was observed. The positive expression of specific proteins further reinforces the possibility of using this approach for assisted reproduction techniques and infertility treatment.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Aline Q. Rodrigues, Victor L. Picolo, Jair T. Goulart, Isabella M. G. Silva, Rayane B. Ribeiro, Beatriz A. Aguiar, Yasmin B. Ferreira, Daniela M. Oliveira, Carolina M. Lucci, Andreza F. de Bem, Fernanda Paulini
Summary: Ovarian tissue cryopreservation followed by transplantation can lead to decreased follicular viability and mitochondrial activity, but remains a feasible strategy for attempts to restore ovarian function.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Arthur Nery da Silva, Michelle Silva Araujo, Fabio Pertille, Adroaldo Jose Zanella
Summary: In the pig industry, there is a growing concern about animal welfare, which has led to the exploration of technologies such as epigenetic biomarkers to predict animal welfare on farms. This review discusses the studies conducted in this area, highlights the benefits of investing in research to enhance animal welfare and consumers' trust, and suggests the potential use of epi-biomarkers for animal welfare certification.
Article
Biochemical Research Methods
Michelle Silva Araujo, Otavio Luis de Oliveira Henriques Paulo, Caroline Scott, Cristiane Sella Paranzini, Viviane Maria Codognoto, Camila de Paula Freitas Dell'Aqua, Frederico Ozanam Papa, Fabiana Ferreira de Souza
Summary: This study characterized the proteome of spermatozoa and seminal plasma in four purebred dog breeds and identified significant proteins related to sperm quality and reproduction. The results suggest that there are differences in semen protein profiles among different canine breeds, which can impact reproductive biotechnologies in dogs.
JOURNAL OF PROTEOMICS
(2022)
Article
Veterinary Sciences
Bianca de Oliveira Horvath-Pereira, Fernanda Paulini, Marco Olivio Sotelo, Ettore Giovanni Leardini, Dhiego Cristiano Tavares, Gustavo Henrique Dona Rodrigues Almeida, Leandro Norberto da Silva Junior, Leticia Beatriz Mazo Pinho, Maria Angelica Miglino, Michelle Silva Araujo
Summary: Shell fractures are a common and traumatic injury in chelonians, often caused by falls, accidents with vehicles, burns, or animal bites. This case report describes a successful treatment using a resin application method for shell fractures in C. carbonarius, which is effective, accessible, affordable, and safe. The use of ethyl-cyanoacrylate mixed with sodium bicarbonate proved to be a bactericidal and non-toxic resin, providing a quick and efficient fix for the fractures.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Fernanda Paulini, Aline R. M. Marangon, Carolina L. Azevedo, Juliana L. M. Brito, Marcelle S. Lemos, Marcelo H. Sousa, Fabiane H. Veiga-Souza, Paulo E. N. Souza, Carolina M. Lucci, Ricardo B. Azevedo
Summary: This study presents a long-term follow-up of rats after a single injection of DMSA-coated magnetite nanoparticles (DMSA-MNP). The results showed that DMSA-MNP administration did not cause significant damage to the rats' health over the course of 300 days. Hematological, biochemical, and ultrasound examinations, as well as histopathology analysis, indicated that the animals remained within normal limits and showed no major alterations in vital organs. This work highlights the safety of using DMSA-MNPs for biomedical applications.
Article
Obstetrics & Gynecology
Aline Q. Rodrigues, Isabella M. G. Silva, Jair T. Goulart, Rayane B. Ribeiro, Beatriz A. Aguiar, Yasmin B. Ferreira, Jessyca Silva, Julliene Larissa S. Bezerra, Carolina M. Lucci, Fernanda Paulini
Summary: This study aimed to investigate the optimal timing for administration of erythropoietin to minimize ischaemic injury in ovarian tissue transplantation. The results showed that treatment with erythropoietin after transplantation improved the viability of ovarian follicles, increased angiogenesis, maintained ovarian follicle proliferation and reduced fibrosis areas in the grafted tissue.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Anatomy & Morphology
Cristiane Naoko Takamine, Hianka Jasmyne Costa de Carvalho, Michelle Silva Araujo, Rafaela Rodrigues Ribeiro, Maria Angelica Miglino, Alan Peres Ferraz de-Melo
Summary: The research on the male reproductive tract of Caiman crocodilus yacare provides valuable insights into its anatomy and histology, paving the way for further studies on reproduction and conservation efforts of this species within the Alligatoridae family.
INTERNATIONAL JOURNAL OF MORPHOLOGY
(2021)
Article
Biology
Charles A. Elder, Jensen S. Smith, Mustafa Almosawi, Ethan Mills, Brett R. Janis, Jonathan A. Kopechek, Willem F. Wolkers, Michael A. Menze
Summary: By using a biomimetics approach and trehalose as a cryoprotective agent, red blood cells can be successfully preserved for long-term storage without the need for time-consuming washing steps.
