Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Materials Science, Multidisciplinary
Danli Zhu, Wanting Bao, Boxuan Wei, Hao Wei, Jinwu Wang, Guangdong Zhou, Xiansong Wang, Shanyu Guo
Summary: In this study, a strategy for the construction of vascularized tissue-engineered adipose tissue was proposed. By integrating a gas-foamed method (soil) and three-dimensional cell spheroids (seeds), adipose tissue regeneration was achieved. The prepared soil significantly enhanced the vascularization of the regenerated tissue, while optimizing the seed cells promoted adipogenesis. This study provides a solution for the regeneration of vascularized adipose tissue and establishes a general research model for the reconstruction of breast defects.
APPLIED MATERIALS TODAY
(2023)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
R. Tevlin, H. desJardins-Park, J. Huber, S. E. DiIorio, M. T. Longaker, D. C. Wan
Summary: Osteoarthritis is a global burden that affects joints and bones. Tissue engineering, specifically adipose-derived stromal cells, shows promise as a treatment option. Advancements in material science allow for improved engraftment of these cells and activation of biophysical signals through designed microstructures.
Article
Biotechnology & Applied Microbiology
Wenpei Dong, Zhicheng Song, Suihong Liu, Ping Yu, Zhipeng Shen, Jianjun Yang, Dongchao Yang, Qinxi Hu, Haiguang Zhang, Yan Gu
Summary: A novel method using thermoresponsive hydrogel and biodegradable polymer was developed to fabricate a composite electrospun scaffold, simulating native extracellular matrix and accelerating cell adhesion and proliferation. Rat adipose-derived stem cells were directionally induced into endothelial cells in the scaffold to enhance early vascularization. Overexpression of HIF-1 alpha/VEGF in ADSCs using a lentivirus system promoted early vascularization in the electrospun scaffolds. Overall, this composite biomimetic scaffold method has potential applications in abdominal wall defect regeneration and repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aida Selaru, Alexandra-Elena Mocanu-Dobranici, Elena Olaret, Raluca-Elena Ginghina, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescu
Summary: In this study, fibrous nanostructured substrates based on fish gelatin enriched with graphene oxide and magnetic nanoparticles were fabricated. The biological properties of these substrates, including cell viability, adhesion, and differentiation, were evaluated. The results showed good interaction between the materials and cells, indicating their potential for tissue engineering applications, with a concentration of 0.5% graphene oxide and magnetic nanoparticles being the most suitable option for biological testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Federica Grilli, Ennio Albanesi, Beatriz Pelacho, Felipe Prosper, Paolo Decuzzi, Daniele Di Mascolo
Summary: Depositing stem cells at the injury site is a clinically relevant strategy for tissue repair and angiogenesis, but insufficient cell engraftment and survival requires the development of new scaffolds. In this study, a microstructured poly(lactic-co-glycolic acid) (PLGA) fabric was investigated as a promising biodegradable scaffold for adipose-derived stem cell (ADSC) tissue integration. The results showed that ADSCs reassembled into spheroidal-like structures on the PLGA fabric, preserving cell viability and promoting a nonlinear actin organization. Moreover, the secretion of specific factors involved in angiogenesis, extracellular matrix remodeling, and stem cell homing was enhanced on the PLGA fabric compared to conventional substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Catherine Ann Martin, Subathra Radhakrishnan, Jose Luis Gomez Ribelles, Omana Anna Trentz, E. A. K. Nivethaa, Mettu Srinivas Reddy, Mohamed Rela, Narayana Kalkura Subbaraya
Summary: The current study investigated a triad composed of adipose tissue derived stem cells and a gelatin/PVA matrix with exclusive ascorbic acid signaling, which was tested as a model for neural tissue engineering. The study found that the scaffold had cytocompatibility and biocompatibility, and its porosity facilitated cell signaling and nutrient and gas exchange. The scaffold successfully directed MSCs to neuronal lineage under specific signaling, and the organotypic model triad could be a simple and effective tissue construct in the field of soft tissue engineering.
REGENERATIVE BIOMATERIALS
(2022)
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Siti Syahira Airuddin, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, Nur Azida Mohd Nasir
Summary: Stem cells, particularly adipose-derived stem cells, have shown promising potential in disease treatment due to their differentiation abilities and secretion of growth factors. However, more research is needed to fully understand the safety and effectiveness of adipose-derived stem cells in medical treatment.
Article
Nanoscience & Nanotechnology
Natsumi Ueda, Shiho Sawada, Fumiya Yuasa, Karen Kato, Koji Nagahama
Summary: This study reports an injectable covalent stem cell-combining biohybrid material for in vivo tissue engineering, which can achieve controlled differentiation and integration with host tissues. By incorporating specific modified stem cells and alginate polymers in a cross-linking agent, the mechanical properties of the material can be adjusted. Experimental results in a mouse muscle defect model demonstrate that this covalent material treatment can effectively promote tissue regeneration with better outcomes compared to control materials.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Somaiah Chinnapaka, Katherine S. Yang, Quinn Flowers, Minhal Faisal, Wayne Vincent Nerone, Joseph Peter Rubin, Asim Ejaz
Summary: Adipose tissue plays a crucial role in metabolic regulation and aging can affect fat storage and stem cell regenerative potential. Metformin treatment improves stemness of adipose-derived stem cells by reducing proliferation and adipocyte differentiation, possibly through inhibiting mTOR and ERK signaling and enhancing autophagy. Further evaluations in animal models and humans are needed for potential clinical application of metformin in rejuvenating aged stem cells.
Review
Biochemistry & Molecular Biology
Ivana Roberta Romano, Floriana D'Angeli, Nunzio Vicario, Cristina Russo, Carlo Genovese, Debora Lo Furno, Giuliana Mannino, Serena Tamburino, Rosalba Parenti, Rosario Giuffrida
Summary: This review discusses the osteogenic and chondrogenic differentiation ability of adipose-derived mesenchymal stromal cells (ASCs) and their potential therapeutic applications in bone and cartilage defects. ASCs can easily differentiate into chondrocyte-like and osteocyte-like elements and they can be used for tissue repair. Various strategies involving ASCs, such as transplantation of freshly harvested cells, in vitro expanded cells, or predifferentiated cells, are being developed globally. Combining ASCs with scaffolds can further enhance their positive effects and contribute to their osteogenic or chondrogenic differentiation.
Article
Biochemistry & Molecular Biology
Elena Filova, Monika Supova, Adam Eckhardt, Marek Vrbacky, Andreu Blanquer, Martina Travnickova, Jarmila Knitlova, Tomas Suchy, Sarka Ryglova, Martin Braun, Zuzana Burdikova, Martin Schatz, Vera Jencova, Maxim Lisnenko, Lubos Behalek, Renata Prochazkova, Radek Sedlacek, Kristyna Kubasova, Lucie Bacakova
Summary: Scaffolds made of degradable polymers, such as collagen, are used for bioartificial vascular grafts or patches. In this study, collagen gel reinforced with collagen particles and adipose tissue-derived stem cells (ASCs) was incubated with different mediums to promote cell differentiation. The constructs were then endothelialised with human umbilical vein endothelial cells (ECs). Immunofluorescence staining and mass spectrometry were performed to evaluate cell differentiation and extracellular matrix proteins. Mechanical properties of the gels with ASCs were measured. The results suggest that PVA_PL_part collagen construct has the highest potential for functional vascular remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Neus Gomila Pelegri, Aleksandra M. M. Stanczak, Amy L. L. Bottomley, Bruce K. K. Milthorpe, Catherine A. A. Gorrie, Matthew P. P. Padula, Jerran Santos
Summary: Neurological diseases are a major cause of disability and death worldwide, but effective treatments are still difficult to develop. Tissue engineering provides a potential solution for testing treatments, but creating accurate brain tissue models remains challenging. This study focused on using adipose-derived stem cells (ADSCs) to create neural models and found that growing ADSCs in a 3D environment with similar mechanical properties to the central nervous system can promote spontaneous neural differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Caterina Marchio, Laura Annaratone, Ana Marques, Laura Casorzo, Enrico Berrino, Anna Sapino
Summary: HER2 is a well-known negative prognostic factor in breast cancer and its status is routinely assessed using immunohistochemistry and in situ hybridization. International recommendations have been developed by experts in the field and are continuously updated based on new experimental and clinical data.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Oncology
Enrico Berrino, Laura Annaratone, Umberto Miglio, Elena Maldi, Chiara Piccinelli, Erica Peano, Davide Balmativola, Paola Cassoni, Alberto Pisacane, Ivana Sarotto, Tiziana Venesio, Anna Sapino, Caterina Marchio
FRONTIERS IN ONCOLOGY
(2020)
Article
Oncology
Jasna Metovic, Laura Annaratone, Alessandra Linari, Simona Osella-Abate, Chiara Musuraca, Francesca Veneziano, Chiara Vignale, Luca Bertero, Paola Cassoni, Nicola Ratto, Alessandro Comandone, Giovanni Grignani, Raimondo Piana, Mauro Papotti
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2020)
Article
Cell Biology
Nelson Rangel, Milena Rondon-Lagos, Laura Annaratone, Andres Felipe Aristizabal-Pachon, Paola Cassoni, Anna Sapino, Isabella Castellano
Article
Respiratory System
Antonino Di Stefano, Roberto Frairia, Fabio L. M. Ricciardolo, Isabella Gnemmi, Antonella Marino Gammazza, Alessio Piraino, Francesco Cappello, Bruno Balbi, Maria Graziella Catalano
CANADIAN RESPIRATORY JOURNAL
(2020)
Letter
Dermatology
S. Osella-Abate, C. Vignale, L. Annaratone, A. Nocifora, L. Bertero, I. Castellano, G. Avallone, L. Conti, P. Quaglino, F. Picciotto, R. Senetta, M. G. Papotti, P. Cassoni, S. Ribero
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2021)
Article
Endocrinology & Metabolism
Letizia Rinella, Benedetta Pizzo, Roberto Frairia, Luisa Delsedime, Giorgio Calleris, Paolo Gontero, Valentina Zunino, Nicoletta Fortunati, Emanuela Arvat, Maria Graziella Catalano
Article
Oncology
Jamal Elhasnaoui, Valentina Miano, Giulio Ferrero, Elena Doria, Antonette E. Leon, Aline S. C. Fabricio, Laura Annaratone, Isabella Castellano, Anna Sapino, Michele De Bortoli
Article
Endocrinology & Metabolism
Jasna Metovic, Chiara Vignale, Laura Annaratone, Simona Osella-Abate, Francesca Maletta, Ida Rapa, Francesco Cabutti, Silvia Patriarca, Marco Gallo, Yuri E. Nikiforov, Marco Volante, Mauro Papotti
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Chemistry, Multidisciplinary
Damiano Pasqualini, Allegra Comba, Laura Annaratone, Virginia Mola, Mario Alovisi, Lorenzo Breschi, Annalisa Mazzoni, Nicola Scotti, Paola Cassoni, Elio Berutti
APPLIED SCIENCES-BASEL
(2020)
Article
Oncology
Roberto Ruiu, Giuseppina Barutello, Maddalena Arigoni, Federica Riccardo, Laura Conti, Giulia Peppino, Laura Annaratone, Caterina Marchio, Giulio Mengozzi, Raffaele Adolfo Calogero, Federica Cavallo, Elena Quaglino
Summary: TNBC patients have poor overall survival due to high recurrence rates, with aggressive cancer stem cells (CSC) playing a key role. TENM4 shows potential as a biomarker and therapeutic target for TNBC treatment.
Article
Oncology
Enrico Berrino, Alberto Bragoni, Laura Annaratone, Elisabetta Fenocchio, Fabrizio Carnevale-Schianca, Lucia Garetto, Massimo Aglietta, Ivana Sarotto, Laura Casorzo, Tiziana Venesio, Anna Sapino, Caterina Marchio
Summary: The study demonstrates the enrichment of gene fusions in colorectal carcinomas, melanomas, and lung adenocarcinomas, confirming the feasibility of this approach in clinical practice. Detection of NTRK gene fusions was performed using different techniques, providing comparative data for effective detection.
Review
Cell Biology
Dora Grassini, Eliano Cascardi, Ivana Sarotto, Laura Annaratone, Anna Sapino, Enrico Berrino, Caterina Marchio
Summary: HER2 has been a successful targeted therapy in breast cancer. Immunohistochemistry with scoring system is used to identify patients who would benefit from anti-HER2 treatment. Following quality controls is necessary for reproducibility.
Review
Cell Biology
Laura Annaratone, Eliano Cascardi, Elena Vissio, Ivana Sarotto, Ewa Chmielik, Anna Sapino, Enrico Berrino, Caterina Marchio
Proceedings Paper
Biophysics
Giada Magni, Federica Cherchi, Elisabetta Coppi, Marco Fraccalvieri, Francesca Tatini, Irene Fusco, Roberto Pini, Anna Maria Pugliese, Felicita Pedata, Antongiulio Mangia, Stefano Gasperini, Francesco S. Pavone, Duccio Rossi Degl'Innocenti, Cristina Tripodi, Domenico Alfier, Lorenzo Targetti, Francesca Rossi
MECHANISMS OF PHOTOBIOMODULATION THERAPY XIV
(2019)