Journal
FOOD RESEARCH INTERNATIONAL
Volume 77, Issue -, Pages 10-16Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2015.03.029
Keywords
Pea seed; Antihypertensive peptides; Protein hydrolysate; Angiotensin converting enzyme; Renin; Spontaneously hypertensive rats
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Funding
- Advanced Foods and Materials Network of Centre of Excellence (AFMNet)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
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The aim of this study was to identify and test the in vivo effectiveness of antihypertensive peptides present in a pea protein hydrolysate. Yellow field pea protein isolate was digested with thermolysin and the hydrolysate passed through a 3 kDa ultrafiltration membrane. The permeate was collected as the PPH-3 and separated on a reverse-phase HPLC column into 10 peptide fractions. Fractions 3-8 showed inhibition of in vitro renin activity that ranged from approx. 21-68% while ACE activity was inhibited by fractions 2-10 in the range of 22-95%. Fraction 7 had the highest dual inhibitions of the two enzymes with 52.16 and 95.17% for renin and ACE, respectively. Fraction 7 was therefore, chosen for peptide characterization by tandem mass spectrometry, which led to sequencing of the following five peptides; LTFPG (534 Da); IIPLEN (698 Da); LSSGDVF (724 Da); IFENLQN (877 Da); and FEGTVFENG (999 Da). LTFPG, IFENLQN and FEGTVFENG had significantly (P < 0.05) higher inhibitions of ACE and renin activities and were orally administered to spontaneously hypertensive rats at a dose of 30 mg/kg body weight. LTFPG showed significantly (P < 0.05) the fastest decrease in systolic blood pressure (SBP) with a maximum of 37 mmHg after 2 h. In contrast the maximum effects of IFENLQN ( 37 mmHg) and FEGTVFENG (-25 mmHg) were observed after 4 h. Overall, the three peptides had significantly (P < 0.05) better SBP-reducing effects than the PPH-3, which gave a maximum of 14 mmHg after 6 h. (C) 2015 Elsevier Ltd. All rights reserved.
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