Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 165, Issue -, Pages 270-277Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2018.02.055
Keywords
3D plasma micro-nanotexturing; Polymer coated glass slides; Microarrays
Funding
- Love Wave Fully Integrated Lab-on-chip Platform for Food Pathogen Detection - LOVE FOOD project [317742]
- EU [68768]
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Glass slides coated with a poly(methyl methacrylate) layer and plasma micro-nanotextured to acquire 3D topography (referred as 3D micro-nanotextured slides) were evaluated as substrates for biomolecule microarrays. Their performance is compared with that of epoxy-coated glass slides. We found that the proposed three-dimensional (3D) slides offered significant improvements in terms of spot intensity, homogeneity, and reproducibility. In particular, they provided higher spot intensity, by a factor of at least 1.5, and significantly improved spot homogeneity when compared to the epoxy-silane coated ones (intra-spot and between spot coefficients of variation ranging between 5 and 15% for the 3D micronanotextured slides and between 25 and 85% for the epoxy-silane coated ones). The latter was to a great extent the result of a strong coffee-ring effect observed for the spots created on the epoxy-coated slides; a phenomenon that was severely reduced in the 3D micro-nanotextured slides. The 3D micronanotextured slides offered in addition higher signal to noise ratio values over a wide range of protein probe concentrations and shelf-life over one year without requirement for specific storage conditions. Finally, the protocols employed for protein probe immobilization were extremely simple. (C) 2018 Elsevier B.V. All rights reserved.
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