Journal
CNS NEUROSCIENCE & THERAPEUTICS
Volume 24, Issue 10, Pages 940-946Publisher
WILEY
DOI: 10.1111/cns.12843
Keywords
curcumin; glucocorticoid receptor; neural stem cell; proliferation; signal transducer and activator of transcription 3
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Funding
- National Natural Science Foundation of China [81471448]
- Natural Science Foundation of Zhejiang Province [Y14H090071]
- Science Technology Department of Zhejiang Province [2012R10073]
- Wenzhou Science & Technology Bureau [Y20150061]
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AimsThis study was to determine whether curcumin had any effect on the proliferation of neural stem cell (NSC), analyze the expression of glucocorticoid receptor (GR), signal transducer and activator of transcription 3 (STAT3), and Notch1 at transcription and protein level, and discuss the related mechanisms. Methods and resultsNSCs were harvested from E15 SD rat brain and cultured. All experiments were performed at the second passage. Cell cytotoxicity, cell viability, and proliferation assays were used to figure out the optimal concentration of curcumin, which can be used for the protein and mRNA studies. The results showed that by downregulation of GR and STAT3 expression, 0.5molL(-1) curcumin exhibited the most pronounced effect in promoting the proliferation of NSCs, which were also induced by antagonists of GR and STAT3, but was inhibited by GR agonist. ConclusionThis study shows that low-dose curcumin stimulates the proliferation of NSCs, which is probably by inhibiting the mRNA and protein expressions of GR and directly or indirectly regulating the STAT3 via the synergistic effect of GR and STAT3 pathways and its related signal pathways.
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