Article
Oncology
Leonardo Franz, Lorenzo Nicole, Anna Chiara Frigo, Giancarlo Ottaviano, Piergiorgio Gaudioso, Tommaso Saccardo, Francesca Visconti, Rocco Cappellesso, Stella Blandamura, Ambrogio Fassina, Gino Marioni
Summary: The mechanism of epithelial-mesenchymal transition (EMT) plays a crucial role in cancer development, tumor progression, and therapy resistance. In laryngeal carcinoma, the predictive value of pN+ status and Slug expression on disease-free survival was identified, supporting the hypothesis of a mutual concurrence of EMT and angiogenesis in driving aggressive phenotype in LSCC. Further studies are needed to characterize the predictive performance of prognostic models based on EMT and angiogenesis.
Article
Genetics & Heredity
Yuzhou Shen, Jicheng TanTai
Summary: Exosome-mediated epithelial mesenchymal transition (EMT) is crucial for cancer metastasis, involving c-Src in exosome secretion and initiation of EMT. Exosomes from metastatic NSCLC cells enhance migration and invasion abilities of primary NSCLC cells, promoting EMT process through the TGF-beta 1 pathway.
Article
Cell Biology
Zhongjian Yu, Xiongjie Zhu, Ying Li, Min Liang, Meijun Liu, Zhile Liu, Lingyu Qin, Xiaoran Wu, Kunpeng Du, Lu Liu, Yong Wang, Yanfang Zheng
Summary: The study shows that in lung adenocarcinoma, circ-HMGA2 promotes cell metastasis through the miR-1236-3p/EMT axis, indicating that it could be a therapeutic target in LUAD.
CELL DEATH & DISEASE
(2021)
Article
Biotechnology & Applied Microbiology
Xiaoxu Liu, Jing Wu, Bo Yang, Yuanyuan Zhao, Yafan Wang, Shuhong Pan, Suibing Miao, Xiaohua Wu
Summary: This study found that the hsa_circ_0005991/miR-30b-3p/Cdc42EP1 axis promotes the epithelial-mesenchymal transition process in endometriosis, providing a theoretical basis for the mechanism exploration and therapy of this disease.
Article
Biotechnology & Applied Microbiology
Huey-En Tzeng, Chih-Hsin Tang, Chun-Hao Tsai, Chih-Hui Chiu, Min-Huan Wu, Yun Yen
Summary: Our study demonstrates that ET-1 induces EMT in human squamous cell carcinoma cell lines and promotes migration and invasion by downregulating miR-489-3p and enhancing TWIST signaling, suggesting a novel therapeutic target in OSCC metastasis.
ONCOTARGETS AND THERAPY
(2021)
Article
Multidisciplinary Sciences
Wenli Zang, Junchao Liu, Fengxue Geng, Dongjuan Liu, Shuwei Zhang, Yuchao Li, Yaping Pan
Summary: This study found that butyrate can promote the migration and invasion of oral squamous cell carcinoma (OSCC) cells by inducing epithelial-mesenchymal transition (EMT), indicating that butyrate may contribute to the metastasis of OSCC.
Article
Oncology
Yuanyuan Jiang, Hanxiang Zhan, Yuqing Zhang, Jingxuan Yang, Mingyang Liu, Chao Xu, Xiao Fan, Junxia Zhang, Zhijun Zhou, Xiuhui Shi, Rajagopal Ramesh, Min Li
Summary: The study revealed that ZIP4 is overexpressed in NSCLC tissues, correlating with poor survival rates. Overexpressed ZIP4 promotes cell migration, invasion, and metastasis, associated with the Snail-N-cadherin signaling axis.
Article
Neurosciences
Yoshiki Sakai, Mayuka Tsunekawa, Kohei Ohta, Tatsuhiro Shimizu, Strahil Iv. Pastuhov, Hiroshi Hanafusa, Naoki Hisamoto, Kunihiro Matsumoto
Summary: In Caenorhabditis elegans hermaphrodites, axon injury activates TLN-1/talin through the cAMP-Epac-Rap GTPase cascade, leading to integrin inside-out activation and subsequent activation of the RhoA-ROCK signaling pathway. The C. elegans integrin signaling network regulates axon regeneration through the Src-RhoGEF-RhoA axis.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Yung-Wei Lin, Yu-Ching Wen, Chi-Hao Hsiao, Feng-Ru Lai, Shun-Fa Yang, Yi-Chieh Yang, Kuo-Hao Ho, Feng-Koo Hsieh, Michael Hsiao, Wei-Jiunn Lee, Ming-Hsien Chien
Summary: SPOCK1 is found to be upregulated in ccRCC tissues and is associated with advanced clinical stages and metastasis. Knockdown and overexpression of SPOCK1 in ccRCC cells affect cell clonogenic ability, migration/invasion ability, and tumor growth and invasion in vitro and in vivo. The mechanism involves the activation of the Snail/Slug-MMP-2 axis to promote EMT and cell motility.
Article
Biotechnology & Applied Microbiology
Guang-Cheng Luo, Lin Chen, Jiang Fang, Zhi-Jian Yan
Summary: This study revealed that hsa_circ_0030586 is significantly upregulated in prostate cancer cells and may promote epithelial-mesenchymal transition (EMT) through the PI3K-AKT signaling pathway. Experiment results showed that interfering with hsa_circ_0030586 could inhibit tumor cell proliferation, migration, and invasion in PCa.
Article
Biochemistry & Molecular Biology
Xigan He, Jinggui Chen, Jiamin Zhou, Anrong Mao, Weiqi Xu, Hongxu Zhu, Qi Pan, Yiming Zhao, Ning Zhang, Longrong Wang, Miao Wang, Zeyang Liu, Weiping Zhu, Lu Wang
Summary: This study identified a newly found lncRNA, EWSAT1, which plays a crucial role in hepatocellular carcinoma (HCC) metastasis. EWSAT1 is significantly upregulated in HCC and is associated with an aggressive phenotype and low patient survival. It promotes proliferation and metastasis of HCC cells by activating the transcription of cancer-related genes through the Hippo-YAP signaling pathway.
Article
Medicine, Research & Experimental
Ke-Da Yang, Ying Wang, Fan Zhang, Bai-Hua Luo, De-Yun Feng, Zhi-Jun Zeng
Summary: This study found that circN4BP2L2 acts as an oncogene in CRC, promoting tumor growth and metastasis by regulating the miR-340-5p/CXCR4 axis. The authors revealed a novel mechanism in the ceRNA regulatory network for CRC progression and identified a potential therapeutic target for CRC treatment.
LABORATORY INVESTIGATION
(2022)
Article
Medicine, Research & Experimental
Yu Zeng, Tianshi Que, Jie Lin, Zhengming Zhan, Anqi Xu, Zhiyong Wu, Cheng Xie, Jie Luo, Shengfeng Ding, Hao Long, Xian Zhang, Ye Song
Summary: This study identified an oncogenic ZEB2/miR-637/HMGA1 signaling axis targeting vimentin that promotes migration and invasion in glioma, leading to an unfavorable prognosis. HMGA1 and vimentin were associated with specific subtypes of glioma.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Hui-Huang Lai, Liang-Yi Hung, Chia-Jui Yen, Hsu-Chin Hung, Ruo-Yu Chen, Yu-Chao Ku, Hang-Tat Lo, Hung-Wen Tsai, Yun-Ping Lee, Tz-Hsuan Yang, Yen-Yu Chen, Yi-Shuian Huang, Wenya Huang
Summary: The overexpression of DNA repair gene NEIL3 in hepatocellular carcinoma (HCC) is associated with tumor development and metastasis. NEIL3 activates specific pathways to promote epithelial-mesenchymal transition, leading to tumor resistance and metastasis. This study suggests that NEIL3 may serve as a potential biomarker for predicting HCC recurrence and clinical intervention.
JOURNAL OF PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Geon-Ho Lee, Jayeon Cheon, Donghee Kim, Hee-Sook Jun
Summary: LPA promotes EMT and renal tubular fibrosis through LPAR1 signaling, downregulating E-cadherin and upregulating vimentin expression. This effect is mediated by regulation of KLF5 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Baharak Ahmaderaghi, Raheleh Amirkhah, James Jackson, Tamsin R. M. Lannagan, Kathryn Gilroy, Sudhir B. Malla, Keara L. Redmond, Gerard Quinn, Simon S. McDade, Tim Maughan, Simon Leedham, Andrew S. D. Campbell, Owen J. Sansom, Mark Lawler, Philip D. Dunne
Summary: We have developed an open source data analysis platform called MouSR, which allows 'wet-lab' users to easily analyze transcriptional data without requiring programming skills. This platform provides a user-friendly interface and a suite of molecular characterization options for rapid transcriptomic analysis, addressing a major bottleneck in biological discovery.
DISEASE MODELS & MECHANISMS
(2022)
Article
Oncology
Vera Grinkevitch, Mark Wappett, Nyree Crawford, Stacey Price, Andrea Lees, Christopher McCann, Katherine McAllister, Jochen Prehn, Jamie Young, Jess Bateson, Lewis Gallagher, Magali Michaut, Vivek Iyer, Aikaterini Chatzipli, Syd Barthorpe, Daniel Ciznadija, Ido Sloma, Amy Wesa, David A. Tice, Lodewyk Wessels, Mathew Garnett, Daniel B. Longley, Ultan McDermott, Simon S. McDade
Summary: Second-generation TRAIL-R2 agonists face two major clinical challenges: lack of predictive biomarkers and development of resistance. This study identifies caspase-8:FLIP(L) and caspase-8:MCL-1 ratios as potential predictive biomarkers for second-generation TRAIL-R2 agonists, and loss of key effectors like FADD and caspase-8 as likely drivers of clinical resistance in solid tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Pathology
Abdo Alnabulsi, Tiehui Wang, Wei Pang, Marius Ionescu, Stephanie G. Craig, Matthew P. Humphries, Kris McCombe, Manuel Salto Tellez, Ayham Alnabulsi, Graeme Murray
Summary: A study developed an effective methodology to identify optimal biomarker combinations for the prognosis of colorectal carcinoma. Six biomarkers were identified as the best combination in terms of prognostic power, and it was significantly associated with overall survival and independent of other clinicopathological parameters. The study also demonstrated the potential of this methodology for biomarker discovery in various biological and clinical studies.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Biochemical Research Methods
Gerard P. Quinn, Tamas Sessler, Baharak Ahmaderaghi, Shauna Lambe, Harper VanSteenhouse, Mark Lawler, Mark Wappett, Bruce Seligmann, Daniel B. Longley, Simon S. McDade
Summary: classifieR is an easy-to-use web application based on R-Shiny, designed to facilitate flexible and rapid single sample annotation of transcriptional profiles from cancer patient samples in laboratories. It provides information on the molecular makeup of samples, as well as analysis of prognosis, druggability, and discovery of new information.
BMC BIOINFORMATICS
(2022)
Article
Gastroenterology & Hepatology
Shania M. Corry, Amy M. B. McCorry, Tamsin R. M. Lannagan, Niamh A. Leonard, Natalie C. Fisher, Ryan M. Byrne, Petros Tsantoulis, Xabier Cortes-Lavaud, Raheleh Amirkhah, Keara L. Redmond, Aoife J. McCooey, Sudhir B. Malla, Emily Rogan, Svetlana Sakhnevych, Michael A. Gillespie, Mark White, Susan D. Richman, Rene-Filip Jackstadt, Andrew D. Campbell, Sarah Maguire, Simon S. McDade, Daniel B. Longley, Maurice B. Loughrey, Helen G. Coleman, Emma M. Kerr, Sabine Tejpar, Timothy Maughan, Simon J. Leedham, Donna M. Small, Aideen E. Ryan, Owen J. Sansom, Mark Lawler, Philip D. Dunne
Summary: This study identifies and validates a HiFi-specific prognostic signature (HPS) based on STAT1-related signaling in the stroma-rich subtype of colon cancer. The HPS is associated with immune cell and antigen processing in stroma-rich colon cancer and treatment with the TLR3 agonist poly(I:C) can enhance the HPS signaling and reduce liver metastases.
Article
Oncology
Vivek Kumar Singh, Md Mostafa Kamal Sarker, Yasmine Makhlouf, Stephanie G. Craig, Matthew P. Humphries, Maurice B. Loughrey, Jacqueline A. James, Manuel Salto-Tellez, Paul O'Reilly, Perry Maxwell
Summary: In this study, a lightweight deep learning model called ICOSeg is proposed to accurately segment the immune-checkpoint biomarker ICOS protein in colon cancer immunohistochemistry slide patches. The proposed model utilizes a convolutional neural network and transformer to extract relevant features from immunohistochemistry images and performs segmentation using an encoder and decoder sub-network. Experimental results show that ICOSeg achieves better segmentation results compared to existing methods with a reduction in parameters.
Article
Oncology
Silvana Debernardi, Oleg Blyuss, Daria Rycyk, Kirtiman Srivastava, Christie Y. Jeon, Hui Cai, Qiuyin Cai, Xiao-Ou Shu, Tatjana Crnogorac-Jurcevic
Summary: This study assessed the predictive performance of a urine biomarker panel for early detection of pancreatic ductal adenocarcinoma (PDAC), and found that the combination with plasma CA19-9 could effectively detect PDAC up to 2 years prior to diagnosis.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Hajrah Khawaja, Rebecca Briggs, Cheryl H. Latimer, Mustasin Rassel, Dary Griffin, Lyndsey Hanson, Alberto Bardelli, Frederica Di Nicolantonio, Simon S. McDade, Christopher J. Scott, Shauna Lambe, Manisha Maurya, Andreas U. Lindner, Jochen H. M. Prehn, Jose Sousa, Chris Winnington, Melissa J. LaBonte, Sarah Ross, Sandra Van Schaeybroeck
Summary: Novel covalent inhibitors of KRASG12C have shown limited response rates in patients with KRASG12C-mutant colorectal cancer. In this study, researchers found that the combination of AZ'1569 and a Bcl-xL inhibitor led to a dramatic and universal apoptosis, suggesting a potential therapeutic strategy for patients with KRAS G12C-mutant colorectal cancer.
MOLECULAR CANCER THERAPEUTICS
(2023)
Article
Pathology
Matthew Phillip Humphries, Victoria Bingham, Fatima Abdullah Sidi, Stephanie Craig, Beatrize Lara, Hesham El-daly, Nicole O'Doherty, Perry Maxwell, Claire Lewis, Stephen McQuaid, James Lyness, Jacqueline James, David R. J. Snead, Manuel Salto-Tellez
Summary: In this study, a validated protocol using multiplex immunofluorescence (mIF) was described for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease. Validated assays originally developed for the detection of SARS-CoV-2 in tissues were applied to map the adaptive immune response at protein and mRNA levels. The mIF panels showed robust, reliable, and quantifiable performance in detecting topographic variations in inflammation related to pathological processes in formalin-fixed, paraffin-embedded pneumonia material.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Cell Biology
Ivana Steiner, Teresita del N. J. Flores-Tellez, Renaud Mevel, Amin Ali, Pengbo Wang, Pieta Schofield, Caron Behan, Nicholas Forsythe, Garry Ashton, Catherine Taylor, Ian G. Mills, Pedro Oliveira, Simon S. McDade, Dietmar M. Zaiss, Ananya Choudhury, Georges Lacaud, Esther Baena
Summary: Conditional deletion of PTEN gene in prostate cells leads to an increase in LY6D+ progenitor cells, resulting in the formation of castration-resistant prostate cancer with unmet clinical need. LY6D+ tumor cells are enriched in high-grade and androgen-resistant prostate cancer, providing clinical evidence for their contribution to advanced disease. Interfering with the MAPK signaling pathway can prevent the progression of castration-resistant prostate cancer.
Article
Oncology
Ross J. J. Porter, Graeme I. I. Murray, Sandra Hapca, Andrew Hay, Stephanie G. G. Craig, Matthew P. P. Humphries, Jacqueline A. A. James, Manuel Salto-Tellez, Daniel P. P. Brice, Susan H. H. Berry, Mairi H. H. McLean
Summary: This study investigates the role of HMGB1 in colorectal cancer and its association with tumor progression, lymph node metastases, and the immune microenvironment. Strong cytoplasmic HMGB1 expression is linked to an 'immune cold' tumor microenvironment and poor survival. HMGB1 may represent a new treatment target for colorectal cancer.
Article
Cell Biology
Aideen McCabe, Oza Zaheed, Simon Samuel McDade, Kellie Dean
Summary: Epithelial ovarian cancer (EOC) is a highly heterogeneous disease with five major histological subtypes. Cell line models that accurately represent these subtypes are essential for pre-clinical EOC research. This study aimed to generate a reference dataset of cell lines representative of the major EOC subtypes and identified lines with high molecular similarity to primary tumors. The findings highlight the importance of selecting suitable cell line models to maximize the clinical relevance of experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
K. Srivastava, K. E. Lines, D. Jach, T. Crnogorac-Jurcevic
Summary: Loss of S100PBP leads to oncogenic transformation of pancreatic cells, and the expression levels of S100P and S100PBP are closely associated with the prognosis of pancreatic cancer patients.
Article
Multidisciplinary Sciences
Fatima Abdullahi G. Sidi, Victoria C. Bingham, Stephen A. McQuaid, Stephanie P. Craig, Richard Turkington, Jacqueline James, Matthew Humphries, Manuel Salto-Tellez
Summary: This study analyzed the expression of immune cells and immune checkpoint biomarkers in a cohort of small bowel adenocarcinoma patients and assessed their impact on survival outcomes. The results showed that high expressions of CD3, CD4, IDO1, and ICOS were significantly associated with progression-free survival, while high PD-L1 expression in tumor cells was significantly associated with overall survival. These findings provide valuable insights for the immunotherapeutic management of small bowel adenocarcinoma patients.
Article
Oncology
Laura Botta, Tomohiro Matsuda, Hadrien Charvat, Chun-Ju Chiang, Wen-Chung Lee, Anna Jacoba van Gestel, Frank Martin, Gijs Geleijnse, Matteo Cellamare, Simone Bonfarnuzzo, Rafael Marcos-Gragera, Marcela Guevara, Mohsen Mousavi, Stephanie Craig, Jessica Rodrigues, Jordi Rubio-Casadevall, Lisa Licitra, Stefano Cavalieri, Carlo Resteghini, Gemma Gatta, Annalisa Trama, RARECAREnet Working Grpo
Summary: Survival of head and neck cancers differs between Europe and Asia, with Europe having lower survival rates. Differences in cancer subsites, histology distribution, and survival were observed between the regions. The variations in survival could be attributed to different factors such as diagnosis period, advanced stage at diagnosis, or availability/access to treatment. The lack of stage and treatment information in cancer registries hinders further exploration of the reasons for survival differences. Federated learning has been proven as a feasible approach for data analysis that addresses the challenges of data sharing, emphasizing the need for collaborative studies involving relevant prognostic factors.
FRONTIERS IN ONCOLOGY
(2023)