Article
Pharmacology & Pharmacy
Amol O. Bajaj, Mark M. Kushnir, Erik Kish-Trier, Rachel N. Law, Lauren M. Zuromski, Alejandro R. Molinelli, Gwendolyn A. McMillin, Kamisha L. Johnson-Davis
Summary: We developed an LC-MS/MS method for monitoring the concentrations of 6-TG and 6-MMP and evaluated the stability of thiopurine metabolites. The method showed good agreement with other methods and revealed differences in metabolite concentrations among different TPMT groups and age groups.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elena Genova, Marianna Lucafo, Marco Pelin, Veronica Di Paolo, Luigi Quintieri, Giuliana Decorti, Gabriele Stocco
Summary: Thiopurines are commonly used in the treatment of chronic autoimmune conditions and leukemia, but may cause gastrointestinal toxicity. This study investigated the metabolite concentrations of thiopurines in intestinal cells, finding associations between metabolite concentrations and cytotoxicity. The findings suggest that short growth times before treatment should be used to study the association between thiopurine metabolite concentrations and cytotoxicity activity in vitro.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Plant Sciences
Kai Uchida, June-Sik Kim, Muneo Sato, Hiromitsu Tabeta, Keiichi Mochida, Masami Yokota Hirai
Summary: This study used metabolomic data obtained by liquid chromatography-mass spectrometry to perform a metabolome GWAS and identified SNPs highly associated with metabolite contents in Arabidopsis thaliana seeds. The study also discovered an unknown methyltransferase gene associated with N-methylhistidine content, and confirmed its importance in N-methylhistidine biosynthesis through knockout and overexpression experiments.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Medical Laboratory Technology
Ahmed B. Bayoumy, Chris J. J. Mulder, Aathavan Loganayagam, Jeremy D. Sanderson, Simon Anderson, Paul J. Boekema, Luc J. J. Derijks, Azhar R. Ansari
Summary: The study findings suggest that TPMT measurements at TG initiation may be beneficial but not essential in daily practice for patients with IBD. TPMT genotypes and phenotypes are both linked to significant differences in 6-TGN levels between metabolic groups. The advantage of TG is that RBC 6-TGN measurements are not essential for monitoring treatments, particularly in countries where diagnostic tests are not readily accessible.
THERAPEUTIC DRUG MONITORING
(2021)
Article
Environmental Sciences
Ankita Das, Tamalika Sanyal, Pritha Bhattacharjee
Summary: The SAM levels are reduced in individuals with arsenic induced skin lesions, and key regulatory genes in the SAM biogenesis pathway are significantly down-regulated in this group. Hypermethylation of the AS3MT promoter leads to its transcriptional repression, potentially contributing to the higher susceptibility in arsenic exposed individuals.
ENVIRONMENTAL RESEARCH
(2021)
Article
Biochemical Research Methods
Nils Muthmann, Petr Spacek, Dennis Reichert, Melissa van Duelmen, Andrea Rentmeister
Summary: Enzymatic modification of the 5'-cap is a versatile approach to modulate mRNA properties by transferring methyl groups or functional moieties at the cap. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is essential for quantifying MTase-mediated cap modifications with sensitivity and accuracy.
Article
Multidisciplinary Sciences
Maciej Noga, Marcin Zakrzewski, Dorota Wianowska, Maciej Gnatowski, Lukasz Paprotny, Kamil Jurowski
Summary: 6-Thioguanine is an immunosuppressive drug used to treat acute leukemia and inflammatory bowel disease. Accurate quantification of 6-Thioguanine in body fluids is essential for effective clinical application. A validated HPLC method has been developed to measure 6-Thioguanine in whole blood samples, providing a reliable approach for drug level monitoring and correct dosing.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Anastasia Alexandridou, Dietrich A. Volmer
Summary: This study investigated the long-term stability of various vitamin D-3 metabolites after storage and found that some derivatization reagents were more stable than others.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
G. Magliocco, F. Le Bloc'h, A. Thomas, J. Desmeules, Y. Daali
Summary: A method for quantifying melatonin and 6-hydroxymelatonin in human overnight urine using liquid chromatography-tandem mass spectrometry has been developed and validated. The study found significant correlations between urinary excretion rates of melatonin and 6-hydroxymelatonin with age, suggesting the potential of the urinary 6-hydroxymelatonin to melatonin ratio as a metric for CYP1A2 activity assessment.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Nafiseh Bagheri, Haider A. J. Al Lawati, Javad Hassanzadeh, Iman Al Lawati
Summary: This article introduces a new method for the extraction and determination of vitamin D3 using microchip-based solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection. A new magnetic adsorbent (IRMOF-3@MLDH) based on Fe3O4 magnetic nanoparticles modified ZnFe layered double hydroxide (LDH)/2-aminoterephthalic acid-Zn2+ metal-organic framework was successfully synthesized and fixed inside a rectangular micro-column. The method showed a broad linear range, high sensitivity, and satisfactory selectivity and recovery values.
Article
Chemistry, Applied
Agnes Dienes-Nagy, Frederic Vuichard, Sandrine Belcher, Marie Blackford, Johannes Roesti, Fabrice Lorenzini
Summary: A fast, sensitive, and reproducible LC-MS/MS method was developed, optimised, and applied for simultaneous quantification of glutathione (GSH), glutathione disulfide (GSSG), and glutathione-S-sulfonate (GSSO3H) in grape juice and wine samples. The results showed that GSH and GSSG were found in grape juice without added SO2, while GSSO3H was detected in must samples treated with SO2. In wine samples, GSSO3H was the dominant form of glutathione, indicating the importance of its quantification. The formation of GSSO(3)H in wine was correlated with the total SO2 level.
Article
Chemistry, Multidisciplinary
Emily C. Ulrich, Catherine L. Drennan
Summary: Cobalamin-dependent S-adenosyl-L-methionine radical methylases are enzymes that use a dual-cofactor system to perform challenging radical methylation reactions. They are part of a larger subgroup of enzymes that also perform difficult ring contractions and radical rearrangements. This subgroup represents a largely unexplored reservoir of diverse chemistry. This Perspective article highlights the efforts to understand the function, mechanism, and structure of TsrM, an unexpected nonradical methylase in this subgroup, and discusses recent achievements in characterizing radical methylase subgroup members.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Food Science & Technology
Xiaoxu Luan, Meiqin Feng, Jian Sun
Summary: The study explored the in vitro antioxidant activity of Lactobacillus plantarum CD101 and its impact on antioxidant peptide formation in fermented sausage. L. plantarum CD101 mainly distributed antioxidant substances on the cell surface and extracellular secretions. The potential benefit of L. plantarum CD101 in inhibiting oxidative rancidity and promoting protein degradation was observed, with a weak effect on promoting antioxidant peptides.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Sara Morello, Sabina Pederiva, Rosa Avolio, Giuseppina Amato, Simona Zoppi, Alessia Di Blasio, Maria Cesarina Abete, Cristina Casalone, Rosanna Desiato, Giuseppe Ru, Daniela Marchis
Summary: The EU has lifted restrictions on using non-ruminant processed animal proteins in aquafeed, with potential future permission for poultry and pig feed, while also evaluating potential hazards related to antibiotic residues. Monitoring studies have detected tetracyclines in processed animal proteins, raising concerns about exposure to antibiotic residues.
Article
Chemistry, Organic
Bohong Lin, Lingling Xiang, Zhijun Yuan, Qi Hou, Yaoping Ruan, Jing Zhang
Summary: Direct derivatization and fluorescence measurement of S-Adenosyl-l-homocysteine (SAH) were achieved using ReACT chemistry, which offers mild reaction conditions and simple operation.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)