Journal
CHEMPHYSCHEM
Volume 19, Issue 14, Pages 1686-1690Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201800225
Keywords
Absorption; Charge transfer; GFP; Ion spectroscopy; Photophysics
Funding
- Danish Council for Independent Research [4181-00048B]
- LHA from the Villum foundation (Villum Experiment Project) [17512]
- Villum Fonden [00017512] Funding Source: researchfish
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Here we uncover the direct effect of a high electric field on the absorption by the Green Fluorescent Protein chromophore anion isolated in vacuo based on gas-phase action spectroscopy. Betaine is a strong molecular dipole that creates an electric field of approximate to 70 MV/cm when attached to the ion at the phenolate oxygen, more than half the actual field from the protein matrix and pointing in the same direction. Nevertheless, the shift in absorption is limited (0.08eV), supporting earlier conclusions, but subject to much debate, that the protein is rather innocent in perturbing the transition energy. The betaine complexes are readily made by electrospray ionization and in contrast to the bare ions, they dissociate after one-photon absorption. Also, electron detachment is not an open channel complicating the bare ion case. As steric constraints are absent in vacuo, the possibility of turning on fluorescence by an electric field can be tested from experiments on complexes with betaine.
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