Engineering enzyme microenvironments for enhanced biocatalysis

Bioinspired design of a hybrid bifunctional enzymatic/organic electrocatalyst for site selective alcohol oxidation

(2018) Louis Lancaster et al.   CHEMICAL COMMUNICATIONS

Substrate Sorting by a Supercharged Nanoreactor

(2018) Yusuke Azuma et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Insertion of a Calcium-Responsive β-Roll Domain into a Thermostable Alcohol Dehydrogenase Enables Tunable Control over Cofactor Selectivity

(2018) Walaa Abdallah et al.   ACS Catalysis

DNA Nanostructure Sequence-Dependent Binding of Organophosphates

(2017) Yingning Gao et al.   LANGMUIR

Toward Rational Design of High-efficiency Enzyme Cascades

(2017) Yifei Zhang et al.   ACS Catalysis

Increasing Enzyme Cascade Throughput by pH-Engineering the Microenvironment of Individual Enzymes

(2017) Yifei Zhang et al.   ACS Catalysis

Block V RTX Domain of Adenylate Cyclase from Bordetella pertussis: A Conformationally Dynamic Scaffold for Protein Engineering Applications

(2017) Beyza Bulutoglu et al.   Toxins

Extreme makeover: Engineering the activity of a thermostable alcohol dehydrogenase (AdhD) fromPyrococcus furiosus

(2016) Kusum Solanki et al.   Biotechnology Journal

Mechanisms of Enhanced Catalysis in Enzyme-DNA Nanostructures Revealed through Molecular Simulations and Experimental Analysis

(2016) Yingning Gao et al.   CHEMBIOCHEM

Protein Allostery and Conformational Dynamics

(2016) Jingjing Guo et al.   CHEMICAL REVIEWS

Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts

(2016) Ladislav Bumba et al.   MOLECULAR CELL

Substrate channelling as an approach to cascade reactions

(2016) Ian Wheeldon et al.   Nature Chemistry

Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion

(2016) Zhao Zhao et al.   Nature Communications

Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade

(2016) Yifei Zhang et al.   Nature Communications

Extreme makeover: Engineering the activity of a thermostable alcohol dehydrogenase (AdhD) fromPyrococcus furiosus

(2016) Kusum Solanki et al.   Biotechnology Journal

Quantitative Packaging of Active Enzymes into a Protein Cage

(2015) Yusuke Azuma et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Diffusion-Limited Cargo Loading of an Engineered Protein Container

(2015) Reinhard Zschoche et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Monobody-mediated alteration of enzyme specificity

(2015) Shun-ichi Tanaka et al.   Nature Chemical Biology

Intrinsically disordered proteins in cellular signalling and regulation

(2015) Peter E. Wright et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Programmable materials and the nature of the DNA bond

(2015) M. R. Jones et al.   SCIENCE

Synthetic protein switches: design principles and applications

(2015) Viktor Stein et al.   TRENDS IN BIOTECHNOLOGY

Tuning Enzyme Kinetics through Designed Intermolecular Interactions Far from the Active Site

(2015) Yingning Gao et al.   ACS Catalysis

Influence of Electrostatics on Small Molecule Flux through a Protein Nanoreactor

(2015) Jeff E. Glasgow et al.   ACS Synthetic Biology

Rational Tailoring of Substrate and Inhibitor Affinity via ATRP Polymer-Based Protein Engineering

(2014) Hironobu Murata et al.   BIOMACROMOLECULES

Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm

(2014) Jinglin Fu et al.   Nature Nanotechnology

Optical Control of Enzyme Enantioselectivity in Solid Phase

(2014) Antoni Bautista-Barrufet et al.   ACS Catalysis

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Industrial use of immobilized enzymes

(2013) Robert DiCosimo et al.   CHEMICAL SOCIETY REVIEWS

Stabilizing biocatalysts

(2013) Andreas S. Bommarius et al.   CHEMICAL SOCIETY REVIEWS

Innovation by homologous recombination

(2013) Devin L Trudeau et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

(2013) Manfred T. Reetz   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Regulation of an Enzyme Cascade Reaction by a DNA Machine

(2013) Ling Xin et al.   Small

Kinetic Enhancements in DNA–Enzyme Nanostructures Mimic the Sabatier Principle

(2013) Jyun-Liang Lin et al.   ACS Catalysis

Enhancement and Modulation of Enzymatic Activity through Higher-Order Structural Changes of Giant DNA-Protein Multibranch Conjugates

(2012) Sergii Rudiuk et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Conformational Switches: From Nature to Design

(2012) Jeung-Hoi Ha et al.   CHEMISTRY-A EUROPEAN JOURNAL

Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures

(2012) Jinglin Fu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Modular exchange of substrate-binding loops alters both substrate and cofactor specificity in a member of the aldo-keto reductase superfamily

(2012) E. Campbell et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Enzymatic biofuel cells utilizing a biomimetic cofactor

(2011) Elliot Campbell et al.   CHEMICAL COMMUNICATIONS

Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior

(2010) Elliot Campbell et al.   BIOTECHNOLOGY AND BIOENGINEERING

Two conformational states of Candida rugosa lipase

(2010) Pawel Grochulski et al.   PROTEIN SCIENCE

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics