Journal
CHEMICAL & PHARMACEUTICAL BULLETIN
Volume 66, Issue 3, Pages 277-285Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.c17-00824
Keywords
methyl-beta-cyclodextrin; hyaluronic acid; adamantane; supramolecular complex; antitumor agent
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Methyl-beta-cyclodextrin (M-beta-CyD) exhibits cytotoxic activity, and has the potentials as an antitumor agent. However, a tumor-selectivity of M-beta-CyD is low, leading to low antitumor activity and the adverse effects. Meanwhile, hyaluronic acid (HA) is known as a promising tumor targeting ligand, because various cancer cells overexpress CD44, a HA-binding glycoprotein. In the present study, to develop a tumor-selective delivery system for M-beta-CyD, we designed a supramolecular complex of M-beta-CyD with adamantane-grafted HA (Ad-HA/M-beta-CyD) and evaluated it as a tumor-selective antitumor agent. M-beta-CyD formed a stable complex with Ad-HA (K-c>10(4)m(-1)). In addition, Ad-HA/M-beta-CyD formed slightly a negative-charged nanoparticle with ca. 140nm of a particle size, indicating the favorable physicochemical properties for antitumor agents. Ad-HA/M-beta-CyD showed the superior cytotoxic activity via CD44-mediated endosomal pathways in HCT116 cells (CD44(+)), a human colon cancer cell line. In addition, cytotoxic activity of Ad-HA/M-beta-CyD was induced by apoptosis. These results suggest that Ad-HA/M-beta-CyD has the potentials as a tumor-selective supramolecular antitumor agent.
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