Article
Entomology
Satoshi Hiroyoshi, Takayuki Mitsunaga, Gadi V. P. Reddy
Summary: This study investigated the effects of temperature, age, and stage on testis development in the diamondback moth. Results showed that testis shrank with age and increasing temperatures, with larval and pupal rearing temperatures also affecting testis development. Field experiments revealed varied testis volume in wild adult males, suggesting a mix of young and old males. Larger adults tended to have larger testis, which were reduced in size after mating. Adult age of diamondback moths in wild populations could be estimated roughly from data on testis volume, temperature, and forewing length.
PHYSIOLOGICAL ENTOMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Leyun Wang, Kayvan Etebari, Gimme H. Walter, Michael J. Furlong
Summary: Temperature has significant effects on insects, including their performance and distribution. While previous studies focused on extreme conditions, such as extreme cold, this study investigated the effects of mild cold on Plutella xylostella. The results showed that females and males had different transcriptomic responses to cold stress, and various genes related to antimicrobial peptides, enzymes, and proteins were differentially expressed in both sexes. This study provides novel insights into the thermal responses of insects and their strategies to cope with low temperatures.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Environmental Sciences
Xuehua Shao, Duo Lai, Weiqiang Xiao, Weiqun Yang, Ying Yan, Shizi Kuang
Summary: In this study, the antifeedant activity of eurycomanone against diamondback moth was evaluated, showing that it was more effective than azadirachtin and could inhibit the development of larvae. Eurycomanone significantly increased the antifeedant index after treating Brassica chinensis L. roots, exhibiting excellent antifeedant activity and systemic absorption.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Gilmar da Silva Nunes, Joice Mendonca de Souza, Dagmara Gomes Ramalho, Sergio Antonio De Bortoli, Ricardo Antonio Polanczyk
Summary: This study examines the impact of Bt-based bioinsecticides on the predation of Euborellia annulipes on Plutella xylostella. The results show that uninfected larvae are consumed less than Bt-infected larvae in a no-choice condition, and uninfected larvae are preferred over Dipel-infected larvae in a free-choice condition. Overall, the preference of the predator is towards uninfected larvae. These findings provide important insights into the interactions between earwigs and Bt.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agronomy
Maria Oplopoiou, Jan Elias, Russell Slater, Chris Bass, Christoph T. Zimmer
Summary: The study revealed that Plutella xylostella displayed significant resistance to emamectin benzoate, which is inherited as an autosomal, recessive trait controlled by one or a few closely linked loci. Additionally, the resistant strain showed resistance to multiple classes of insecticides, indicating a broad multi-resistance profile.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Sereyboth Soth, Travis R. Glare, John G. Hampton, Stuart D. Card, Jenny J. Brookes
Summary: This study evaluated multiple Beauveria isolates for the control of diamondback moth and found that certain isolates were highly effective in killing the larvae. The research also determined that low-virulent Beauveria isolates showed better control of the pest compared to mixtures containing high-virulent isolates.
Article
Entomology
Jing Wang, Xiaobin Zheng, Jiangjiang Yuan, Shuaiyu Wang, Baoyun Xu, Shaoli Wang, Youjun Zhang, Qingjun Wu
Summary: The study revealed that the diamondback moth has developed varying degrees of resistance to multiple insecticides, with the highest resistance observed against beta-cypermethrin. The resistance levels were highest in Midu region.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhen Zhang, Lei Xiong, Chao Xie, Lingling Shen, Xuanhao Chen, Min Ye, Linyang Sun, Xiaozhen Yang, Shuyuan Yao, Zhen Yue, Zhengjiao Liang, Minsheng You, Shijun You
Summary: The CRISPR/Cas9 system is an efficient tool for reverse genetics validation and provides a new perspective on target gene analysis in cell lines for the development of biotechnology tools. Through optimization and improvement, the CRISPR/Cas9 system was successfully applied to cell lines of the diamondback moth, Plutella xylostella, laying the foundation for further genetic and molecular studies in insects, particularly gene function analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Debashis Roy, Sayan Sau, Samrat Adhikary, Abhisek Biswas, Sujan Biswas, Gautam Chakraborty, Pijush Kanti Sarkar
Summary: The diamondback moth Plutella xylostella has developed resistance to fluxametamide, a new neuroactive compound. The resistance can potentially increase significantly after continuous exposure, and the specific activities of cytochrome P450 and glutathione S-transferase play a role in the resistance development. This information is crucial for formulating effective fluxametamide application and resistance management guidelines.
Article
Agronomy
Takehiko Yamanaka, Satoshi Kitabayashi, Akiya Jouraku, Hiroyuki Kanamori, Seigo Kuwazaki, Masaaki Sudo
Summary: A feasibility trial of genomics-based diagnosis of insecticide resistance in diamondback moth populations was conducted in Nagano Prefecture, Japan. The study revealed that mutations related to resistance against pyrethroids, organophosphates, and diamides were prevalent, while mutations against cyantraniliprole, Bt toxin (Cry1Ac), and spinosyns were scarce, suggesting their continued effectiveness. The results of the genomics-based diagnosis were consistent with those of bioassays, and resistance development tendencies were generally uniform across Nagano.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Tiansheng Liu, Xue Zhan, Yuan Yu, Shaozhen Wang, Cong Lu, Guifang Lin, Xiangyu Zhu, Weiyi He, Minsheng You, Shijun You
Summary: Insect biogenic amines mediate behavioral and physiological processes through binding to biogenic amine receptors (BARs) in the G-protein-coupled receptor superfamily. This study cloned and characterized 17 genes encoding putative biogenic amine receptor proteins from the global pest Plutella xylostella, providing insights into potential target sites for pest control. The molecular properties, expression profiles, and pharmacological characterization of these BARs, including dopamine receptor PxDOP2, were investigated, offering valuable information and resources.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Li-Jun Cai, Li-Shuang Zheng, Yu-Ping Huang, Wei Xu, Min-Sheng You
Summary: This study identified 39 putative OBP genes from the diamondback moth genome and transcriptome sequences, and investigated their expression patterns across different developmental stages, sexes, and tissues. The results showed that most OBPs were highly expressed in adult antennae, with certain OBPs being regulated by mating activities and host plant feeding.
Article
Horticulture
Francisco Ruben Badenes-Perez, David G. Heckel
Summary: Based on the experiments conducted, the diamondback moth, a major pest of cruciferous crops, does not show preference for the abaxial or adaxial leaf surfaces or for host plants based on their diet. However, larval preference is influenced by glucosinolate content, indicating that crops with higher glucosinolate content are more susceptible to damage by the diamondback moth. These findings are significant in the management of this pest.
Article
Agriculture, Multidisciplinary
Benjie Li, Ying Yan, Guangkai Yao, Ling Zhang, Fei Lin, Hanhong Xu
Summary: A series of novel pyrazoloquinazolines were synthesized, and pyrazoloquinazoline 5a was found to have high insecticidal activity against the diamondback moth without cross-resistance to fipronil. Electrophysiological experiments suggested that 5a acts on the GABAR and GluCl. The potency of 5a on PxGluCl was 15-fold higher than fipronil, explaining the lack of cross-resistance. Downregulation of PxGluCl transcription significantly enhanced the insecticidal activity of 5a on the diamondback moth.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Xiaofeng Xia, Qian Wang, Geoff M. Gurr, Liette Vasseur, Shuncai Han, Minsheng You
Summary: The diamondback moth (DBM) has adapted to the chemical defenses of Brassicaceae plants, but its role in the DBM's adaptation to plant defense compounds is unknown. In this study, the secondary metabolites of radish seedlings were identified and found to reduce the growth of DBM larvae lacking gut bacteria. However, the re-introduction of Enterobacter dominated gut microbiota counteracted this effect. The bacterium Enterobacter not only degrades the flavonoid kaempferol, but also colonizes the gut epithelium, forming a protective biofilm.