Journal
FITOTERAPIA
Volume 105, Issue -, Pages 102-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2015.06.013
Keywords
Fungal plasma membrane H+-ATPase; High-resolution screening; HPLC-HRMS-SPE-NMR; Uvaria chamae
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Funding
- Danish Research Council for Strategic Research - Food and Health [0606-00384B]
- Carlsberg Foundation
- Apotekerfonden
- Danish Agency for Science, Technology and Innovation via the National Research Infrastructure funds
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In our ongoing efforts of finding natural fungicides to fight food and feed spoilage during production and storage, the antifungal potential of Ghanaian Uvaria chamae P. Beauv. was investigated, with emphasis on plant metabolites targeting the fungal plasma membrane (PM) H+-ATPase. Ethyl acetate extract of U. chamae was subjected to high-resolution fungal PM H+-ATPase inhibition screening followed by structural elucidation by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR). This led to identification of a series of uncommon o-hydroxybenzylated flavanones and chalcones, i.e., chamanetin (8), isochamanetin (9), isouvaretin (10), uvaretin (11), dichamanetin (12), and diuvaretin (15). Preparative-scale isolation of the active metabolites allowed determination of IC50 values for inhibition of the PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. These revealed a strong correlation between o-hydroxybenzyl substituents and PM H+-ATPase activity, with dichamanetin being the most potent compound, but showing moderate activity in the fungal growth inhibition assays. (C) 2015 Elsevier B.V. All rights reserved.
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