Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots

The inulin-type fructan was obtained by DEAE anion exchange chromatography from C. pilosula Nannf. var. modesta (Nannf.) L. T. Shen, after optimized extract condition, which was established by response surface methodology, designed using Box-Behnken factorial design and the optimum condition were: extracting 2.5 h with ratio of solvent to material 40 mL/g at 100 degrees C, twice. The maximum extraction yield was 20.6 +/- 0.2%. It was confirmed as beta-(2-1) linkage fructan, with terminal glucose, and with a degree of polymerization of 2-17 (DPav = 6), shown by the results of methanolysis, methylation, nuclear magnetic resonance and molecular weight determination. The prebiotic activity was proven on account of stimulation effect on Lactobacillus and pH reduction of medium in vitro. The results indicated that the inulin from C. pilosula could be used as a potential natural source of prebiotics.