Article
Cell Biology
Aoqi Li, Xiaoning Han, Linhong Deng, Xiang Wang
Summary: Tunneling nanotubes (TNTs) are important for intercellular communication and have potential therapeutic significance in diseases. The mechanical properties and stability of TNTs are crucial for their efficiency, with F-actin and cadherin connections determining their tensile and flexural strength.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sangwoo Shim, Raman Goyal, Alexios A. Panoutsopoulos, Olga A. Balashova, David Lee, Laura N. Borodinsky
Summary: The balance between neural stem cell proliferation and neuronal differentiation is crucial for the development of the nervous system. We found that Sonic hedgehog (Shh) enhances Ca2+ activity at the cilia of developing Xenopus laevis embryos, which promotes neuronal differentiation and inhibits proliferative signaling. These findings provide potential targets for the treatment of brain tumors and neurodevelopmental disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Immunology
Maksim V. Baranov, Manoj Kumar, Stefano Sacanna, Shashi Thutupalli, Geert van den Bogaart
Summary: The size, shape, rigidity, and surface roughness of pathogens are important parameters affecting cellular uptake and immune responses, particularly in inflammasome activation and T cell activation. Understanding how the physical properties of particles influence immune responses can assist in the design of more effective vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Manuela Voelkner, Thomas Kurth, Jana Schor, Lynn J. A. Ebner, Lara Bardtke, Cagri Kavak, Joerg Hackermueller, Mike O. Karl
Summary: The optimized protocol for high yield mouse retinal organoids facilitates the assessment of key parameters, showing reliable completion of retinogenesis and maintenance for at least 60 days in culture with continued maturation. Activation of sonic hedgehog signaling during development increased retinal epithelial length in the organoid system, supporting organoid maturation, stability, and reproducibility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Elle C. Roberson, Ngan Kim Tran, Anushka N. Godambe, Harrison Mark, Michelle Nguimtsop, Trinity Rust, Elizabeth Ung, LeCaine J. Barker, Rebecca D. Fitch, John B. Wallingford
Summary: Decades of research have shown that the mammalian estrous cycle is regulated by cycling steroid hormones. However, the signaling mechanisms connecting hormonal action and the physical changes in the uterus during the cycle are still unclear. In this study, gene expression during all stages of the mouse estrous cycle was analyzed, revealing that several genetic programs responsible for tissue morphogenesis in developing embryos display cyclical patterns of expression. Furthermore, it was found that most of the genes involved in the Hedgehog signaling pathway are transcribed cyclically in the uterus. Disruption of the Hedgehog receptor smoothened not only affects the normal thickening of the endometrial lining but also induces abnormal deformation of the uterine smooth muscle. These findings provide insights into the mechanisms underlying normal uterine remodeling and cyclical gene expression.
Article
Oncology
Catena Kresbach, Lea Holst, Melanie Schoof, Tara Leven, Carolin Goebel, Sina Neyazi, Jacqueline Tischendorf, Carolin Loose, Antonina Wrzeszcz, Timur Yorgan, Stefan Rutkowski, Ulrich Schueller
Summary: The study evaluated the use of intraventricular Vismodegib treatment for medulloblastoma in mice and found that this treatment method can rapidly induce complete or partial remission of tumors, and significantly improve symptom-free survival in a dose-dependent manner.
Article
Immunology
Diana C. Yanez, Eleftheria Papaioannou, Mira M. Chawda, Jasmine Rowell, Susan Ross, Ching-In Lau, Tessa Crompton
Summary: Pharmacological inhibition of smoothened reduces Th2 cell infiltration and alleviates the severity of allergic asthma by decreasing the induction of Shh, IL-4, and IL-13, as well as lowering serum IgE levels and expression of inflammation-related genes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrea B. Alber, Hector A. Marquez, Liang Ma, George Kwong, Bibek R. Thapa, Carlos Villacorta-Martin, Jonathan Lindstrom-Vautrin, Pushpinder Bawa, Feiya Wang, Yongfeng Luo, Laertis Ikonomou, Wei Shi, Darrell N. Kotton
Summary: This study successfully generated a mouse induced pluripotent stem cell (iPSC) line carrying a lung-specific mesenchymal reporter/lineage tracer, and identified the signaling pathways necessary for specifying lung mesenchyme. Through co-culture with lung epithelial cells, three-dimensional organoids with juxtaposed layers of epithelium and mesenchyme were formed, and functional crosstalk between the two cell types was observed.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Jenny A. A. Klein, Zhen Li, Sanjeev Rampam, Jack Cardini, Amara Ayoub, Patricia Shaw, Angela L. L. Rachubinski, Joaquin M. M. Espinosa, Ella Zeldich, Tarik F. F. Haydar
Summary: The study found that intellectual disability in individuals with Down syndrome is related to changes in early brain development, including the differentiation of neural progenitor cells and the formation of myelin. By activating the Sonic hedgehog pathway, researchers were able to study how trisomy 21 affects early neural precursors. They found that the dysregulation of OLIG2 gene expression and the blockage in NPC transition in brain-like trisomic NPCs can be normalized by increasing the concentration of a SHH pathway agonist.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biology
Erumbi S. S. Rangarajan, Emmanuel W. W. Smith, Tina Izard
Summary: Cell attachment is crucial and mediated by cadherin receptors binding and alpha-catenin interaction. Alpha-catenin modulates cell migration and adhesion by linking junctions and plasma membrane to actin cytoskeleton. CryoEM structures provide mechanistic insights into alpha-catenin function and its role in cell adhesion and disease.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Irene Delgado, Giovanna Giovinazzo, Susana Temino, Yves Gauthier, Aurelio Balsalobre, Jacques Drouin, Miguel Torres
Summary: Meis1 and Meis2 are homeodomain transcription factors that regulate organogenesis by cooperating with Hox proteins. Inactivation of Meis1/2 in the lateral plate mesoderm leads to limb agenesis in mouse embryos, with Meis and Tbx factors co-binding and co-regulating enhancers of Fgf10. Limbs with deleted Meis alleles show skeletal hypoplasia and agenesis of posterior elements due to their early role in establishing limb antero-posterior prepattern.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Yuwei Sun, Luyue Wang, Tong Zhu, Bailin Wu, Guangqin Wang, Zhengnan Luo, Chao Li, Wu Wei, Zhiyong Liu
Summary: This study uses single-cell RNA sequencing to reveal the transcriptomes of mouse otic tissues at early embryonic ages, and identifies several genes playing crucial roles in otic neurogenesis. The findings have important implications for our understanding of early inner ear development.
Article
Multidisciplinary Sciences
Vladimir Jovasevic, Hui Zhang, Farahnaz Sananbenesi, Anita L. Guedea, Kizhake Soman, John E. Wiktorowicz, Andre Fischer, Jelena Radulovic
Summary: It has been shown that genes associated with cytoskeleton and extracellular matrix remain dynamically expressed even at remote time points, with primary cilium and collagen genes playing a significant role in stabilizing perineuronal nets to promote memory formation. Nonsynaptic, primary cilium-mediated mechanisms are essential for the persistence of context memory.
Article
Biochemistry & Molecular Biology
Feifei Shi, Yixin Xu, Shuhui Zhang, Zhenhua Fu, Qian Yu, Shaozhi Zhang, Mingzhu Sun, Xin Zhao, Xizeng Feng
Summary: This study found that DBDPE has toxic effects on embryonic development and has transgenerational interference effects. Maternal exposure to DBDPE may result in behavioral and cognitive memory impairments in the next generation of mice. These findings emphasize the reproductive toxicity of DBDPE and suggest that environmental pollutants may initiate adult diseases during the embryonic stage.
Article
Developmental Biology
Flora Crozet, Christelle Da Silva, Marie-Helene Verlhac, Marie-Emilie Terret
Summary: Research shows that in mammalian oocytes, F-actin does not require myosin-X to transmit forces to the spindle, which differs from most mitotic cells.
Article
Cell & Tissue Engineering
Mi Ok Kim, Jung Min Ryu, Han Na Suh, Soo Hyun Park, Yeon-Mok Oh, Sang Hun Lee, Ho Jae Han
STEM CELLS AND DEVELOPMENT
(2015)
Article
Reproductive Biology
Min Jung Kim, Hyun Ju Oh, Geon A. Kim, Han Na Suh, Young Kwang Jo, Yoo Bin Choi, Dong Hoon Kim, Ho Jae Han, Byeong Chun Lee
Article
Multidisciplinary Sciences
Sohee Jun, Youn-Sang Jung, Han Na Suh, Wenqi Wang, Moon Jong Kim, Young Sun Oh, Esther M. Lien, Xi Shen, Yoshihisa Matsumoto, Pierre D. McCrea, Lei Li, Junjie Chen, Jae-Il Park
NATURE COMMUNICATIONS
(2016)
Article
Cell Biology
Han Na Suh, Moon Jong Kim, Youn-Sang Jung, Esther M. Lien, Sohee Jun, Jae-Il Park
Article
Cell Biology
Moon Jong Kim, Bo Xia, Han Na Suh, Sung Ho Lee, Sohee Jun, Esther M. Lien, Jie Zhang, Kaifu Chen, Jae-Il Park
DEVELOPMENTAL CELL
(2018)
Article
Pharmacology & Pharmacy
Han Na Suh, Ho Jae Han
BRITISH JOURNAL OF PHARMACOLOGY
(2015)
Article
Multidisciplinary Sciences
Han Na Suh, Sohee Jun, Ah-Young Oh, Mrinal Srivastava, Sunhye Lee, Cullen M. Taniguchi, Songlin Zhang, Won Sup Lee, Junjie Chen, Bum-Joon Park, Jae-Il Park
SCIENTIFIC REPORTS
(2016)
Article
Cell Biology
Youn-Sang Jung, Sohee Jun, Moon Jong Kim, Sung Ho Lee, Han Na Suh, Esther M. Lien, Hae-Yun Jung, Sunhye Lee, Jie Zhang, Jung-In Yang, Hong Ji, Ji Yuan Wu, Wenqi Wang, Rachel K. Miller, Junjie Chen, Pierre D. McCrea, Scott Kopetz, Jae-Il Park
NATURE CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Han Na Suh, Young Kyu Kim, Ju Young Lee, Goo-Hwa Kang, Jeong Ho Hwang
Summary: Minipigs are a good animal model for studying inflammation due to their similar physiology to humans. Through conventional and novel analytic tools, inflammation in Chicago Miniature Swine (CMS) minipig induced by LPS was detected in various organs with liver and kidney damage observed in serum chemistry and histopathology. Immune cell distribution increased in spleen and lung, NF-kB-target gene expression was upregulated in lung and kidney during acute inflammation, and cytokine mRNA and levels were elevated in PBMC and serum. The systemic inflammation affects multiple organs and can be detected through various methods.
Review
Neurosciences
Seung Won Kim, Hye-Ji Woo, Eun Hee Kim, Hyung Sun Kim, Han Na Suh, Soo-hyun Kim, Jae-Jin Song, Noviana Wulansari, Minji Kang, Se-Young Choi, Su Jeong Choi, Won Hyuk Jang, Jungbin Lee, Ki Hean Kim, Wongyoung Lee, Sung Hyun Kim, Jinhee Yang, Jangbeen Kyung, Hyun-Seob Lee, Sang Myun Park, Mi-Yoon Chang, Sang-Hun Lee
Summary: The study successfully generated midbrain organoids from human pluripotent stem cells for preparing cells for Parkinson's disease therapy. These cells exhibited stable midbrain characteristics with low batch-to-batch variations, showing potential for clinical application due to their therapeutic capacities.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Young Kyu Kim, Ju Young Lee, Han Na Suh
Summary: Through mouse models, it was found that lipopolysaccharide and ovalbumin can increase immune cell infiltration in tissues, activate NF-kappa B, increase cytokine levels in serum, and stimulate JAK2/STAT3 signaling, promoting tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
Ju Young Lee, JuKyung Lee, Sung Ho Lee, Jeong Ho Hwang, Han Na Suh
Summary: We examined the toxicity of Pelargonium sidoides extract on MDCK cells and found that it reduced cell viability in a dose-dependent manner. The extract caused mitochondrial dysfunction, increased ROS levels, and upregulated the apoptosis-related gene BBC3. It also disrupted cytoskeleton signaling and promoted apoptosis.
TOXICOLOGICAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Young Kyu Kim, JuKyung Lee, Hyeon-Young Kim, Sung-Hwan Kim, Jeong Ho Hwang, Han Na Suh
Summary: This study used minipigs as a model for dermal research and found that age and body weight are key factors to consider when developing an Ova-induced AD minipig model.
LABORATORY ANIMAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Hong-Soo Lee, Da-Hee Kim, Sung-Hwan Kim, Min-Sung Kang, Han Na Suh
Summary: This study on nonhuman primates found that different anesthetic combinations have varying effects on intraocular pressure, with male monkeys having higher IOP than females and no significant difference between the right and left eye.
LABORATORY ANIMAL RESEARCH
(2021)