Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 49, Issue 2, Pages 429-442Publisher
SPRINGER
DOI: 10.1016/j.bjm.2017.05.010
Keywords
Bacillus; Cellulases; Conserved signature indels
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Funding
- C.S.I.R., New Delhi
- U.G.C., New Delhi
- DST-purse grant from the University of Delhi
- R & D grant from the University of Delhi
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Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and p-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times beta-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein sequences of three Bacillus spp. as query, revealed their similarities with members of nine bacterial phyla and to Eukaryota (represented by Arthropoda and Nematoda), and also highlighted of a convergent and divergent evolution from other enzymes of different substrate [(1,3)-linked beta-D-glucans, xylan and chitosan] specificities. Characteristic conserved signature indels were observed among members of Actinobacteria (7 aa insert) and Firmicutes (9 aa insert) that served as a potential tool in support of their relatedness in phylogenetic trees. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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