Article
Veterinary Sciences
Ivana Piredda, Loris Bertoldi, Giuseppe Benvenuto, Bruna Palmas, Aureliana Pedditzi, Pierangela Pintore, Valentina Chisu
Summary: This study found that leptospiral infection was widespread in dogs in Sardinia, with some urine samples testing positive for Leptospira DNA and a new Leptospira species isolated from one dog's urine for the first time. Further research is needed to determine if healthy dogs shedding leptospires in their urine could pose a zoonotic risk for humans in the region.
VETERINARY SCIENCES
(2021)
Article
Veterinary Sciences
Cassia Moreira Santos, Gabrielle Cristini Del Rigo Santos Dias, Alexya Victoria Pinheiro Saldanha, Stephanie Bergmann Esteves, Adriana Cortez, Israel Barbosa Guedes, Marcos Bryan Heinemann, Amane Paldes Goncales, Bruno Alonso Miotto
Summary: Leptospirosis is an endemic zoonosis in Brazil with a significant impact on human and animal health. This study identified the causative agent for severe cases of canine leptospirosis through isolation and characterization of strains in a highly endemic area. Multiple diagnostic approaches were used to confirm acute canine leptospirosis, highlighting the importance of utilizing different methods for diagnosis.
BMC VETERINARY RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Ricardo Sant'Anna da Costa, Maria Isabel N. Di Azevedo, Ana Luiza dos Santos Baptista Borges, Luiza Aymee, Gabriel Martins, Walter Lilenbaum
Summary: This study assessed the long-term protective effects of vaccination against leptospirosis in dogs, showing that all commercial vaccines were successful at eliciting a long-lasting immune response and partially effective in protecting against kidney infection.
Article
Agriculture, Dairy & Animal Science
Ricardo Sant'Anna da Costa, Maria Isabel N. Di Azevedo, Ana Luiza dos Santos Baptista Borges, Filipe Anibal Carvalho-Costa, Gabriel Martins, Walter Lilenbaum
Summary: Dogs, known hosts of Leptospira interrogans, were found to shed the pathogen in their urine with unexpectedly prevalent Icterohaemorrhagiae serogroup, despite remaining clinically asymptomatic. Asymptomatic shedding of leptospires in dogs, particularly virulent strains, represents a significant public health risk that cannot be ignored.
Article
Veterinary Sciences
Francesca Grippi, Valeria Blanda, Paola Galluzzo, Manuel Bongiorno, Carmela Sciacca, Francesca Arcuri, Rosalia D'Agostino, Ilenia Giacchino, Francesca Gucciardi, Mario D'Incau, Cristina Bertasio, Alessandra Torina, Annalisa Guercio
Summary: In this study, an acute case of leptospirosis in a puppy housed at a municipal kennel was reported, and diagnostic investigations were conducted on all dogs in the kennel. Laboratory tests including microagglutination test, real-time PCR, and MLST were performed. The puppy tested positive for Leptospira interrogans Icterohaemorrhagiae, while no other dogs showed clinical signs of leptospirosis.
VETERINARY SCIENCES
(2023)
Article
Pathology
Monika Hilbe, Horst Posthaus, Giulia Paternoster, Simone Schuller, Michelle Imlau, Hanne Jahns
Summary: In the past 20 years in Switzerland, dogs with suspected acute leptospirosis have shown severe glomerular changes that were previously unreported. This study describes these significant glomerular pathological changes and investigates their association with leptospirosis.
VETERINARY PATHOLOGY
(2023)
Article
Microbiology
Francesca Grippi, Vincenza Cannella, Giusi Macaluso, Valeria Blanda, Giovanni Emmolo, Francesco Santangelo, Domenico Vicari, Paola Galluzzo, Carmela Sciacca, Rosalia D'Agostino, Ilenia Giacchino, Cristina Bertasio, Mario D'Incau, Annalisa Guercio, Alessandra Torina
Summary: This study investigates the prevalence of Leptospira spp. in stray animals, finding higher infection rates in dogs compared to cats. Pathogenic Leptospira spp. were also detected in the urine and blood of stray cats.
Review
Veterinary Sciences
Anna Cecilia Trolesi Reis Borges Costa, Raisa Abreu Braganca Colocho, Carine Rodrigues Pereira, Andrey Pereira Lage, Marcos Bryan Heinemann, Elaine Maria Seles Dorneles
Summary: This systematic review investigated the prevalence of leptospirosis among stray and sheltered dogs worldwide. The results showed that Leptospira spp. are present in unowned dogs globally, with the highest prevalence observed in the Americas. The most commonly used diagnostic test was the microscopic agglutination test, and the most common serovars were Canicola, Icterohaemorrhagiae, Grippotyphosa, and Pomona.
ANIMAL HEALTH RESEARCH REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Rina Nonogaki, Anna Iijima, Kumiko Kawamura, Shizuo Kayama, Motoyuki Sugai, Tetsuya Yagi, Yoshichika Arakawa, Yohei Doi, Masahiro Suzuki
Summary: The study developed a rapid and simple genotyping method for Klebsiella pneumoniae, called PCR-based open reading frame typing (POT), which was validated for 192 strains in clinical settings. The distribution patterns of ORFs among K. pneumoniae correlated well with multilocus sequence typing (MLST), enabling the identification of closely related species and key antimicrobial resistance and hypervirulence genes as part of POT. The POT method is a valuable tool for monitoring transmission events and facilitating molecular epidemiological analysis in infection control and hospital epidemiology investigations.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Xinchun Zheng, Pengyuan He, Ruihua Zhong, Gongqi Chen, Jinyu Xia, Chunna Li
Summary: A case of Weil's disease with HIV infection was reported in this study. Leptospira interrogans was identified via mNGS in the bronchoalveolar lavage fluid of the patient. The patient achieved complete recovery after receiving targeted antimicrobial therapy.
Article
Microbiology
Bangchuan Hu, Yue Tao, Ziqiang Shao, Yang Zheng, Run Zhang, Xuejing Yang, Jingquan Liu, Xi Li, Renhua Sun
Summary: This study compared the diagnostic utility of ddPCR and mNGS for critically ill patients with suspected BSIs. It found that ddPCR was faster and more sensitive for common isolated pathogens and AMR genes, while mNGS detected a broader range of pathogens, including viruses. Based on their respective strengths and limitations, ddPCR is more suitable for rapid detection of common isolated pathogens and AMR genes, while mNGS is more appropriate for diagnosing BSIs when causative pathogens cannot be identified by traditional methods.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Peter Paul C. Lim, Lisa M. Stempak, Sindhoosha Malay, LeAnne N. Moore, Sree Sarah S. Cherian, Ankita P. Desai
Summary: The clinical utility of 16S rRNA sequencing in pediatric culture-negative infections was investigated in this retrospective study. The results showed that 16S rRNA sequencing had a significant impact on decreasing the number of antibiotics used in the treatment of these infections. Pulmonary specimens had the highest likelihood of identifying a pathogen compared to other specimen types.
Review
Microbiology
Linoj Samuel
Summary: Blood cultures have been the main tool in clinical microbiology laboratories for a long time, but there are still limitations in identifying the causative agent in septic patients. While molecular technologies have revolutionized various areas of clinical microbiology, they have not yet provided a viable alternative to blood cultures. Recently, there has been increased interest in utilizing new approaches to address this challenge.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Hyo Lim Hong, Laure Flurin, Matthew J. Thoendel, Matthew J. Wolf, Matthew P. Abdel, Kerryl E. Greenwood-Quaintance, Robin Patel
Summary: This study compared the performance of 16S rRNA gene-based targeted metagenomic sequencing (tNGS) and shotgun metagenomic sequencing (sNGS) in detecting and identifying pathogens in sonicate fluid from failed total hip arthroplasty (THA) and total knee arthroplasty (TKA). The study found that tNGS had a higher positive percent agreement with sonicate fluid culture compared to sNGS, and detected potential pathogens in culture-negative PJIs. The study concludes that tNGS is a potential diagnostic tool for identifying PJI pathogens in sonicate fluid, with similar performance characteristics to sNGS.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
David Conrad, Alexandra Kehl, Tobias Mueller, Robert Klopfleisch, Heike Aupperle-Lellbach
Summary: This study evaluated the prognosis and treatment of canine cutaneous mast cell tumours in the toe, finding that over half of the tumours had poor prognosis indicators and mutations in the c-kit gene. French Bulldogs were more likely to develop malignant tumours. This study contributes to better understanding of digital mast cell tumours in canines.
Article
Agriculture, Dairy & Animal Science
Pedro G. Castelo, Lucas A. Rodrigues, Michele de P. Gabardo, Roberto M. Carvalho Guedes, Andrea M. Moreno, Fernanda M. Coura, Marcos Bryan Heinemann, Bruno O. Rosa, Ana P. L. Brustolini, Itallo C. S. Araujo, Dalton de O. Fontes
Summary: This study demonstrates that adding spray-dried plasma (SDP) to post-weaning diets can improve growth performance and reduce bacterial shedding in pigs.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Veterinary Sciences
Natalia C. Gaeta, Alexandre Hellmeister, Fabio S. Possebon, Joao P. Araujo, Marcos B. Heinemann
Summary: This study investigated the genomic and microbiological characteristics of a Staphylococcus epidermidis strain isolated from the urine of a guinea pig in Brazil. The strain exhibited a broad range of resistance and virulence genes, and was genetically related to clinical isolates from Australia and the United States. These findings highlight the importance of detecting this microorganism as a urinary tract infection agent in exotic pets, and the need for updated knowledge on detection and therapeutic management among veterinarians.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Review
Agriculture, Dairy & Animal Science
Matheus S. Monteiro, Bruno B. D. Muro, Rafaella F. Carnevale, Andre P. Poor, Kawany M. Araujo, Carlos H. C. Viana, Glen W. Almond, Andrea M. Moreno, Cesar A. P. Garbossa, Diego F. Leal
Summary: The provision of nesting materials to farrowing sows has been shown to reduce redirected nest-building behaviors, improve farrowing traits and maternal behavior, and decrease pre-weaning mortality. However, the effects of nesting material vary depending on the type and quantity of material provided and the farrowing system.
APPLIED ANIMAL BEHAVIOUR SCIENCE
(2023)
Article
Agronomy
Maysa Serpa Goncalves, Elaine Maria Seles Dorneles, Marcos Bryan Heinemann, Maria Aparecida Vasconcelos Paiva E Brito, Alessandro de Sa Guimaraes
Summary: This study evaluated the genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolates from dairy cows in Minas Gerais, Brazil. The results showed high resistance rates for penicillins and tetracyclines and great genetic diversity among the isolates.
Article
Agriculture, Dairy & Animal Science
Renan Braga Paiano, Jeannine Bonilla, Guilherme Pugliesi, Andrea Micke Moreno, Pietro Sampaio Baruselli
Summary: Clinical endometritis (CE) and subclinical endometritis (SE) are two uterine diseases that can affect milk production and fertility in dairy cows during the puerperium period. This study found that cows with CE and SE had lower conception rates and milk production, but no effect on milk production during 305 DIM. Furthermore, cows with CE and SE but negative bacteria isolation did not have impaired reproductive performance and milk production during the postpartum period.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Microbiology
Ana Paula Vilhena Beckman Pinho, Fernando Ferreira, Jeferson Jaco Fuck, Jefferson Pinto de Oliveira, Ricardo Augusto Dias, Jose Henrique Hildebrand Grisi-Filho, Marcos Bryan Heinemann, Evelise Oliveira Telles, Jose Soares Ferreira Neto
Summary: Glanders is a bacterial disease that affects mainly equids and is caused by Burkholderia mallei. While it has been eradicated in certain regions, it continues to occur sporadically in Asia, Africa, the Middle East, and South America. A study conducted in Brazil found a low prevalence of infected properties and seropositive animals, but highlighted the need for reassessment of disease control strategies. The study emphasized the importance of involving both public and private entities in combating the disease.
Article
Infectious Diseases
Maria Gabriela Xavier de Oliveira, Marcos Paulo Vieira Cunha, Luisa Zanolli Moreno, Andre Becker Simoes Saidenberg, Monica Aparecida Midolli Vieira, Tania Aparecida Tardelli Gomes, Andrea Micke Moreno, Terezinha Knobl
Summary: This study aimed to characterize the pathogenicity and antimicrobial resistance profiles of A. butzleri strains isolated from poultry meat sold at retail markets in Sao Paulo, Brazil. The results showed that a significant percentage of the isolates were resistant to multiple drugs, highlighting the need for improved diagnostic tools and specific control strategies to ensure the safety of poultry meat.
Article
Agriculture, Dairy & Animal Science
Fernando N. Souza, Kamila R. Santos, Jose A. Ferronatto, Eduardo M. Ramos Sanchez, Bruno Toledo-Silva, Marcos B. Heinemann, Sarne De Vliegher, Alice M. M. P. Della Libera
Summary: This study investigated whether different staphylococcal and mammaliicoccal species and strains can induce B and T lymphocyte proliferation and production of interleukin-17A (IL-17A) and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMCs) of nulliparous, primiparous, and multiparous dairy cows. Flow cytometry was used to measure lymphocyte proliferation, specific antibodies were used to identify different lymphocyte populations, and the PBMC culture supernatant was used to measure IL-17A and IFN-gamma production. The results showed that certain strains of Staphylococcus aureus and Staphylococcus chromogenes triggered T lymphocyte proliferation and increased IL-17A and IFN-gamma production. Conversely, Mammaliicoccus fleurettii and other strains had no effect on lymphocyte proliferation. Multiparous cows had higher B lymphocyte proliferation and cytokine production compared to primiparous and nulliparous cows. Phytohemagglutinin M-form specifically stimulated T cell proliferation.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Bruno Fontana Soares Ferreira, Marcelo Luis Barros, Fernando Ferreira, Adelmar Rocha, Ricardo Augusto Dias, Jose Henrique Hildebrand Grisi Filho, Marcos Bryan Heinemann, Evelise Oliveira Telles, Guilherme Camargo Alevate, Jose Soares Ferreira Neto
Summary: The control measures for bovine brucellosis implemented in the state of Rondonia, Brazil, have yielded significant economic benefits, with a reduction in the prevalence of infected herds and an increase in milk production through heifer vaccination and serological testing.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Veterinary Sciences
Joao Eduardo Cavalcanti Brito, Beatriz Gagete Verissimo de Mello, Natalia Carrillo Gaeta, Juliana Maria Nunes Batista, Tiago Roberto Brito, Washington Carlos Agostinho, Paulo Eduardo Brandao, Marcos Bryan Heinemann, Ricardo Augusto Dias
Summary: This study investigated the influence of urbanization on bat species richness, relative abundance, and pathogen occurrence. The results showed that higher levels of urbanization led to lower bat species richness and higher relative abundance of captured bats. Noise level, luminosity, and relative humidity were correlated with bat abundance. Additionally, the study found that bats play a significant role in the circulation of pathogens of medical and veterinary interest. These findings are important for promoting harmonious coexistence between humans, bats, and domestic animals in areas with varying levels of anthropization.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Environmental Sciences
Daphne Wrobel Goldberg, Marina Molinas Alcala, Daniela Farias da Nobrega, Fabio Parra Sellera, Solange Fonseca, Jose Soares Ferreira Neto, Denise Batista Nogueira, Marcos Bryan Heinemann
Summary: A case of disseminated mycobacteriosis caused by M. gordonae in a free-ranging sea turtle was reported in Rio de Janeiro, Brazil. The turtle exhibited granulomas and caseous exudate in multiple organs, and acid-fast bacteria were observed in various tissues. The source of the Mycobacterium remains unknown, but increased surveillance of mycobacterial species in wild sea turtles is crucial to understand their role as causative agents of infections.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Microbiology
Guilherme S. de Moura, Eneas de Carvalho, Eduardo M. Ramos Sanchez, Fabio P. Sellera, Michele F. S. Marques, Marcos B. Heinemann, Sarne De Vliegher, Fernando N. Souza, Rinaldo A. Mota
Summary: The discovery of mecA and mecC-positive mammaliicocci from American continent represents a new reservoir of antimicrobial resistance genes, indicating a possible emergence of M. sciuri as a globally important pathogen. These M. sciuri strains carried a wide range of antimicrobial resistances, virulence-associated genes, and belonged to a globally disseminated branch associated with farm animals, companion animals, and food. The findings highlight the need to monitor M. sciuri under the One Health approach due to its potential spread at the human-animal-environment interface.
VETERINARY MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Tatiana Nunes de Azevedo Romanowski, Ricardo Augusto Dias, Marcos Bryan Heinemann, Stephani Felix Carvalho, Tamires Ataides Silva, Andressa da Silva Martins, Geovanna Domingues da Cunha Caetano, Alvaro Ferreira Junior, Jandra Pacheco dos Santos, Ana Carolina Borsanelli
Summary: A high prevalence of leptospirosis was found in equine herds in the state of Goias, Central Brazil. The presence of seropositive animals and positive farms suggests the need for environmental sanitation measures and health education to prevent and control this disease in horses.
VETERINARY SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tatiana F. P. de Oliveira, A. F. Antonio Junior, Anapolino m. de Oliveira, Marcelo F. Camargos, Marcos B. Heinemann
Summary: This study analyzed the genetic relationships between different types of viral contaminants found in cell cultures, calf serum, and standard strains. The results showed that the contaminants included Bovine viral diarrhea virus, Hobi-like viruses, and Classical swine fever virus.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)