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Biology
Shufang Cai, Bin Hu, Xiaoyu Wang, Tongni Liu, Zhuhu Lin, Xian Tong, Rong Xu, Meilin Chen, Tianqi Duo, Qi Zhu, Ziyun Liang, Enru Li, Yaosheng Chen, Jianhao Li, Xiaohong Liu, Delin Mo
Summary: In this study, the molecular and cellular dynamics of pig embryonic myogenesis were studied through single-cell resolution analysis of gene expression and chromatin accessibility. The differentiation trajectory of pig skeletal muscle development was identified, and critical regulators such as EGR1 and RHOB were identified through integrative analysis.
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Biochemistry & Molecular Biology
Keren Long, Xiaokai Li, Duo Su, Sha Zeng, Hengkuan Li, Yu Zhang, Biwei Zhang, Wenying Yang, Penghao Li, Xuemin Li, Xun Wang, Qianzi Tang, Lu Lu, Long Jin, Jideng Ma, Mingzhou Li
Summary: This study investigates the chromatin interactions of eight myogenic marker genes during myogenesis using 4C-seq. The results reveal dynamic chromatin interactions of these marker genes during differentiation and identify significant interaction sites related to muscle development. Functional genomic screening identifies putative active enhancers and luciferase reporter assays validate the functionality of eight enhancers. Furthermore, epigenome editing and RNA-Seq experiments demonstrate the regulatory role of Myog enhancers in Myog expression and myogenic differentiation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
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Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
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Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Review
Cell Biology
Bahareh Hekmatnejad, Michael A. Rudnicki
Summary: Skeletal muscle has a remarkable regenerative capacity mediated by satellite cells. Transplantation assays provide insights into the functional heterogeneity of satellite cells, and strategies to improve transplantation efficiency and monitor cell behavior are discussed. Recent approaches to enhance the transplantation potential of muscle stem cells are also addressed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
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Health Care Sciences & Services
Karyn G. Robinson, Adam G. Marsh, Stephanie K. Lee, Jonathan Hicks, Brigette Romero, Mona Batish, Erin L. Crowgey, M. Wade Shrader, Robert E. Akins
Summary: This study examined the DNA methylation differences in myoblasts and myotubes derived from satellite cells in patients with spastic cerebral palsy (CP). The results showed significant DNA methylation differences in these cells, suggesting potential new targets for studying the mechanisms contributing to muscle dysregulation in spastic CP.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
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Cell Biology
Katja Stange, Amir Keric, Andreas Friese, Monika Roentgen
Summary: This study used a mid-scale incubator and bioreactor system to produce spheroids from primary porcine muscle cells and C2C12 cells, and compared their characteristics. The results showed that both cell types formed spheroids after 24 hours, but C2C12 cells had larger spheroid diameter and lower number of aggregates. After dissociation and differentiation, both cell types were able to proliferate and differentiate into the myogenic lineage. This suggests that the mid-scale incubator and bioreactor system is suitable for spheroid cultivation of primary muscle cells.
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Biochemistry & Molecular Biology
Li Li, Chenyu Qin, Yuan Chen, Wei Zhao, Qi Zhu, Dinghui Dai, Siyuan Zhan, Jiazhong Guo, Tao Zhong, Linjie Wang, Jiaxue Cao, Hongping Zhang
Summary: The interplay between coding and noncoding factors is crucial for muscle growth and development. This study reveals a mutual activation between the long noncoding RNA H19 and MyoD, and describes the regulatory mechanisms involved.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Dairy & Animal Science
Jinsol Jeong, Kwang-Hwan Choi, Seung-Hun Kim, Dong-Kyung Lee, Jong-Nam Oh, Mingyun Lee, Gyung Cheol Choe, Chang-Kyu Lee
Summary: In this study, pig embryonic fibroblasts were transdifferentiated into skeletal muscle through overexpression of the MYOD1 gene and modulation of signaling pathways. The use of signaling molecules with MYOD1 overexpression led to the formation of myotubes and activation of muscle markers in PEFs. This myogenic reprogramming protocol could be valuable for muscle disease models and cultured meat production in regenerative medicine and cellular agriculture.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Biology
Sheng Wang, Yinlong Liao, Haoyuan Zhang, Yunqi Jiang, Zhelun Peng, Ruimin Ren, Xinyun Li, Heng Wang
Summary: This study elucidates the crucial role of Tcf12 in muscle development and regeneration, showing that its deletion in MuSCs leads to muscle weight loss and delayed regeneration. Tcf12 is essential for chromatin remodelling in MuSCs and works with MYOD to regulate the expression of myogenic genes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mengjia Hu, Yukai Lu, Song Wang, Zihao Zhang, Yan Qi, Naicheng Chen, Mingqiang Shen, Fang Chen, Mo Chen, Lijing Yang, Shilei Chen, Dongfeng Zeng, Fengchao Wang, Yongping Su, Yang Xu, Junping Wang
Summary: The study identifies CD63 as a novel functional marker of HSCs in mice, showing that high CD63 expression is associated with more quiescent HSCs with robust self-renewal and myeloid differentiation abilities. CD63 deficiency leads to reduced HSC numbers, impaired quiescence, and increased sensitivity to irradiation and 5-fluorouracil treatment. Further investigations reveal that CD63 interacts with TGF beta receptors to sustain TGF beta signaling activity and regulate HSC quiescence.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Food Science & Technology
Hyeong-Seok Kang, Ji Hye Park, Joong-Hyuck Auh
Summary: This study investigated the effects and active compounds of silkworm pupae on C2C12 muscle differentiation. The protein of silkworm pupae was extracted using sonication after defatting with hexane. The silkworm pupae protein hydrolysate effectively promoted C2C12 myogenic differentiation without cytotoxicity.
Article
Cell Biology
Nasa Xu, Jianbo Wu, Jose L. Ortiz-Vitali, Yong Li, Radbod Darabi
Summary: Recent advancements in reprogramming somatic cells into induced pluripotent stem cells (iPSCs) have allowed for the efficient derivation of skeletal myogenic progenitors from human pluripotent stem cells (ESCs/iPSCs) using a directed differentiation method without the need for gene integration or modification. This method induces mesoderm and subsequently myotomal progenitors, enabling the generation of skeletal myogenic progenitors in a short time frame with high efficiency.
Article
Multidisciplinary Sciences
P. Nayak, A. Colas, M. Mercola, S. Varghese, S. Subramaniam
Summary: This research compares the transcriptomic profiles of hiPSC lines of different myogenic specification levels, revealing temporal differences and the role of β-catenin transcriptional cofactors in mediating cellular interactions and external cues during differentiation.
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Geriatrics & Gerontology
Patricia Sosa, Elena Alcalde-Estevez, Ana Asenjo-Bueno, Patricia Plaza, Natalia Carrillo-Lopez, Gemma Olmos, Susana Lopez-Ongil, Maria Piedad Ruiz-Torres
Summary: Hyperphosphatemia impairs myogenic differentiation and leads to muscle fibrosis, with studies in older mice demonstrating a close relationship between age-related hyperphosphatemia and the decrease in myogenic factors and increase in fibrotic factors.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
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Genetics & Heredity
Katja Stange, Claus-Eric Ott, Mareen Schmidt-von Kegler, Gabriele Gillesen-Kaesbach, Stefan Mundlos, Katarina Dathe, Petra Seemann
JOURNAL OF HUMAN GENETICS
(2015)
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Genetics & Heredity
Katja Stange, Julie Desir, Naseebullah Kakar, Thomas D. Mueller, Birgit S. Budde, Christopher T. Gordon, Denise Horn, Petra Seemann, Guntram Borck
ORPHANET JOURNAL OF RARE DISEASES
(2015)
Article
Multidisciplinary Sciences
Claudia Miersch, Katja Stange, Silvio Hering, Martin Kolisek, Torsten Viergutz, Monika Roentgen
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Claudia Miersch, Katja Stange, Monika Roentgen
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2018)
Article
Biochemistry & Molecular Biology
Julia Haupt, Alexandra Deichsel, Katja Stange, Cindy Ast, Renata Bocciardi, Roberto Ravazzolo, Maja Di Rocco, Paola Ferrari, Antonio Landi, Frederick S. Kaplan, Eileen M. Shore, Carsten Reissner, Petra Seemann
HUMAN MOLECULAR GENETICS
(2014)
Article
Biochemistry & Molecular Biology
Katja Stange, Tino Thieme, Karen Hertel, Silke Kuhfahl, Andreas R. Janecke, Hildegunde Piza-Katzer, Maila Penttinen, Marja Hietala, Katarina Dathe, Stefan Mundlos, Elisabeth Schwarz, Petra Seemann
JOURNAL OF MOLECULAR BIOLOGY
(2014)
Article
Anatomy & Morphology
Laura Hildebrand, Mareen Schmidt-von Kegler, Maria Walther, Petra Seemann, Katja Stange
DEVELOPMENTAL DYNAMICS
(2019)
Article
Multidisciplinary Sciences
K. Stange, C. Miersch, G. Sponder, M. Roentgen
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Katja Stange, Hellen Elisa Ahrens, Julia von Maltzahn, Monika Roentgen
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2020)
Article
Multidisciplinary Sciences
Bianka Grunow, Katja Stange, Ralf Bochert, Katrin Toenissen
Summary: The growth and muscle metabolism of rainbow trout and maraena whitefish at slaughter size were examined, revealing significant interspecies differences, such as smaller diameter cells and higher stem cell proportion in red muscle of rainbow trout. These differences suggest a higher degree of hypertrophic growth in rainbow trout compared to maraena whitefish.
Article
Multidisciplinary Sciences
Yaolu Zhao, Elke Albrecht, Katja Stange, Zeyang Li, Johannes Schregel, Quentin L. Sciascia, Cornelia C. Metges, Steffen Maak
Summary: This study found that glutamine supplementation can improve muscle growth in low birth weight piglets by stimulating cell proliferation both in muscle tissue and in vitro myogenic cell culture, with minimal impact on the expression of muscle growth regulatory genes.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Katja Stange, Amir Keric, Andreas Friese, Monika Roentgen
Summary: This study used a mid-scale incubator and bioreactor system to produce spheroids from primary porcine muscle cells and C2C12 cells, and compared their characteristics. The results showed that both cell types formed spheroids after 24 hours, but C2C12 cells had larger spheroid diameter and lower number of aggregates. After dissociation and differentiation, both cell types were able to proliferate and differentiate into the myogenic lineage. This suggests that the mid-scale incubator and bioreactor system is suitable for spheroid cultivation of primary muscle cells.
Article
Biochemistry & Molecular Biology
Katja Stange, Toni Schumacher, Claudia Miersch, Rose Whelan, Martina Kluenemann, Monika Roentgen
Summary: Optimal supply of L-methionine promotes muscle growth, whereas over-supplementation has adverse effects. This study examines the effects of different methionine supplements on cell growth, viability, ROS production, and mitochondrial bioenergetics in mouse and quail myoblasts. Results show that all supplements stimulate cell growth, but high levels of L-methionine or DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA) retard growth and increase extracellular H2O2 levels, indicating oxidative stress. DL-HMTBA oversupplementation also leads to adaptive changes in mitochondrial functionality. Quail cells are better able to cope with methionine oversupplementation than mouse cells.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Laura Hildebrand, Katja Stange, Alexandra Deichsel, Manfred Gossen, Petra Seemann
CELLULAR SIGNALLING
(2017)