Article
Immunology
Na Li, Jiahong Wang, Xianquan Zhan
Summary: This study identified two reliable immune-related gene signature models that were significantly associated with prognosis and tumor microenvironment cell infiltration in LUAD and LUSC, respectively. Evaluation of these models could help predict response to immunotherapy and guide immunotherapy strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Chiao-Yun Lin, Kai-Yun Wu, Lang-Ming Chi, Yun-Hsin Tang, Huei-Jean Huang, Chyong-Huey Lai, Chi-Neu Tsai, Chia-Lung Tsai
Summary: SH3PXD2A/TKS5 is a scaffold protein that promotes invadopodia formation and regulates cell migration, and is often overexpressed in various cancers. The mechanisms underlying SH3PXD2A-mediated cellular migration signaling pathway are still unknown. This study found that starvation-induced autophagy or treatment with the MTOR inhibitor RAD001 increased SH3PXD2A expression in ovarian cancer cells. SH3PXD2A formed a complex with ULK1 and MTOR, and ULK1 phosphorylated SH3PXD2A at specific serine residues, affecting its stability and binding to cellular components. These findings suggest that the ULK1-SH3PXD2A-MMP14 axis may play a role in regulating the aggressiveness of ovarian cancer and could be a potential therapeutic target.
Article
Spectroscopy
Yafeng Qi, Lin Yang, Bangxu Liu, Li Liu, Yuhong Liu, Qingfeng Zheng, Dameng Liu, Jianbin Luo
Summary: In this study, Raman spectroscopy and deep learning were utilized for accurate and stain-free intraoperative diagnosis. By treating Raman spectral signals as sequences and converting them into two-dimensional Raman spectrograms using short-time Fourier transform as input, high performance deep learning models were achieved, surpassing traditional methods in accuracy and sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Medicine, General & Internal
Piaopiao Li, Hui Yuan, Xuemei Kuang, Tingting Zhang, Lei Ma
Summary: This study identified core genes and their associations with clinical characteristics and patient outcomes in LUSC and LUAD. These findings have important implications for early diagnosis and treatment of LUSC and LUAD.
Article
Biology
Md. Asad Ullah, Sayka Alam, Abu Tayab Moin, Tanvir Ahamed, Abdullah Mohammad Shohael
Summary: In this study, researchers investigated the molecular mechanism of the impact of COVID-19 in lung cancer patients. They identified several differentially expressed genes in common between lung cancer and COVID-19 patients, and found that COVID-19 may affect the expression of cancer-associated genes. Furthermore, the study suggests that COVID-19 may predispose lung cancer patients to other diseases and identifies potential diagnostic and molecular targets for this group of patients.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Chengdi Wang, Qiuxiao Yu, Tingting Song, Zhoufeng Wang, Lujia Song, Ying Yang, Jun Shao, Jingwei Li, Yinyun Ni, Ningning Chao, Li Zhang, Weimin Li
Summary: An investigation into the immune landscape is crucial for immunotherapy strategies and predicting clinical responses in non-small-cell lung cancer patients. Using single-cell RNA sequencing, researchers have identified immune signature differences between lung adenocarcinoma and lung squamous cell carcinoma, the two major subtypes of NSCLC. They found that cytotoxic and effector T and NK cells, as well as distinct macrophage subtypes, play central roles in the immune microenvironment heterogeneity between the two subtypes. This comprehensive depiction of immune heterogeneity could aid in personalized treatment design.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Health Care Sciences & Services
Talip Zengin, Tugba Onal-Suzek
Summary: This study aimed to identify key genes and pathways for diagnostic and prognostic prediction for lung adenocarcinoma and squamous cell carcinoma through analysis of genomic and transcriptomic variations, providing potential personalized oncology biomarkers.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Matthew Uzelac, Yuxiang Li, Jaideep Chakladar, Wei Tse Li, Weg M. Ongkeko
Summary: The human microbiome is a collection of microbial species that exist throughout the body and regulate various bodily functions. The impact of archaea species in the microbiome has not been well explored, despite their potential metabolic interaction with host cells. This study investigates the correlation of specific archaeal species with lung adenocarcinoma and lung squamous cell carcinoma, and identifies potential molecular targets associated with the archaea microbiome. The abundance of archaeal species in lung tissue samples was assessed, revealing altered abundance in cancerous samples. Analysis of gene expression and oncogenic signaling pathways provides insights into the mechanisms of cancer development. Additionally, a machine-learning algorithm was developed to accurately distinguish lung cancer patients from healthy individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Respiratory System
Jing Ning, Shenyi Jiang, Xiaoxi Li, Yang Wang, Xuhong Deng, Zhiqiang Zhang, Lijie He, Daqing Wang, Youhong Jiang
Summary: The expression of GPC3 in NSCLC, LUAD, and LUSC showed significant differences, and was significantly correlated with pathological N, pathological T, gender, and tumor stage. Three-gene and two-gene risk-score models were established for LUAD and LUSC respectively, with the potential to improve the prognosis of these carcinomas.
BMC PULMONARY MEDICINE
(2021)
Article
Oncology
Shuye Lin, Cuimeng Tian, Jianhui Li, Bin Liu, Teng Ma, Keqiang Chen, Wanghua Gong, Ji Ming Wang, Jiaqiang Huang
Summary: Disruption in mucin 22 (MUC22) expression and epigenetic alterations show distinct patterns in lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) in NSCLC, indicating potential for molecular subtyping and prognostic value in precision medicine.
Article
Multidisciplinary Sciences
Takashi Yamashita, Yusuke Takanashi, Asuka Uebayashi, Mikako Oka, Kiyomichi Mizuno, Akikazu Kawase, Soho Oyama, Takuya Kitamoto, Minako Kondo, Shiho Omori, Hong Tao, Yutaka Takahashi, Takumi Sakamoto, Tomoaki Kahyo, Haruhiko Sugimura, Mitsutoshi Setou, Norihiko Shiiya, Kazuhito Funai
Summary: In patients with unresectable non-small cell lung cancer, a novel diagnostic method independent of tissue morphology or conventional immunohistochemistry (IHC) markers is required. This study analyzed the lipid profiles of resected primary lung adenocarcinoma (ADC) and squamous cell carcinoma (SQCC) specimens and identified novel candidate lipid markers with high diagnostic abilities for ADC and SQCC. These lipid markers may contribute to a more accurate diagnosis and subsequent treatment strategy for unresectable NSCLC.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Quewang Liu, Yueying Wang, Meiyu Duan, Yusi Fan, Xingyuan Pan, Shuai Liu, Qiong Yu, Lan Huang, Fengfeng Zhou
Summary: The study found that sex-specific models outperformed sex-independent models in detecting early-stage lung cancers, and Venn plots indicated that females and males shared only a few transcriptomic biomarkers of early-stage lung cancers. Therefore, sex information plays an important role in optimizing disease diagnosis models.
Article
Endocrinology & Metabolism
Wen Wang, Hui Liu, Guoli Li
Summary: The study found significant clinical differences between LUAD and LUSC, with LUSC potentially being more malignant than LUAD. These findings may have potential implications for clinical practice.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Dahua Liu, Min An, Guimin Wen, Yanan Xing, Pu Xia
Summary: With the high incidence and mortality rate of lung cancer, it is of great importance to analyze the molecular mechanism and identify early diagnosis markers. SLC3A2 protein, which is highly expressed in lung cancer, plays a crucial role in tumorigenesis and development, and has the potential to become a new target for tumor treatment. The levels of SLC3A2 in serum and tissue can serve as prognostic markers for lung cancer and provide valuable insights for diagnosis, treatment, and prognosis.
Article
Oncology
Leilei Shen, Juntang Guo, Weidong Zhang, Chaoyang Liang, Han Chen, Yang Liu
Summary: The necessity of performing 4L lymph node dissection (LND) remains uncertain and controversial. This study aimed to analyze the clinicopathological and survival outcomes of 4L LND from the perspective of histology.
FRONTIERS IN ONCOLOGY
(2023)
Article
Critical Care Medicine
Katharina Jandl, Leigh M. Marsh, Ayse Ceren Mutgan, Slaven Crnkovic, Francesco Valzano, Diana Zabini, Julia Hoffmann, Vasile Foris, Elisabeth Gschwandtner, Walter Klepetko, Helmut Prosch, Holger Flick, Luka Brcic, Izidor Kern, Akos Heinemann, Horst Olschewski, Gabor Kovacs, Grazyna Kwapiszewska
Summary: This study identified a functional link between natural killer T (NKT) cell activation and vascular fibrosis in patients with pulmonary fibrosis-associated pulmonary hypertension (PF-PH). Activation of NKT cells ameliorated vascular remodeling and right ventricular systolic pressure in a mouse model of PF-PH. Furthermore, activation of the STAT1-CXCL9-CXCR3 axis by NKT cell activation reduced collagen deposition in human pulmonary arterial smooth muscle cells and precision-cut lung slices. These findings suggest a novel therapeutic strategy for targeting vascular fibrosis in interstitial lung diseases.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Medical Laboratory Technology
Luka Brcic, Nolwenn Le Stang, Florian Gallob, Daniel Pissaloux, Ruth Sequeiros, Sandrine Paindavoine, Jean Claude Pairon, Marie Karanian, Sanja Dacic, Nicolas Girard, Andrew Churg, Franck Tirode, Francoise Galateau-Salle
Summary: Immunohistochemistry for MTAP and p16 can serve as a surrogate for CDKN2A HD in pleural mesotheliomas. Loss of nuclear p16 (<1% staining) and cytoplasmic MTAP (<=30% staining) indicates poor prognosis in pleural mesothelioma patients.
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2023)
Article
Medicine, General & Internal
Oliver Illini, Hannah Fabikan, Maximilian Johannes Hochmair, Christoph Weinlinger, Dagmar Krenbek, Luka Brcic, Ulrike Setinek, Angelika Terbuch, Gudrun Absenger, Selma Konjic, Arschang Valipour
Summary: This study retrospectively assessed the clinicopathological features and standard-of-care treatment responses in patients with KRAS(G12C)-mutated advanced non-small cell lung cancer. The study highlights the poor outcomes in this patient population and emphasizes the need for new treatment options and specific molecular testing.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Suyang Li, Guihuan Qiu, Jun Wu, Jiaxin Ying, Haiyi Deng, Xiaohong Xie, Xinqing Lin, Zhanhong Xie, Yinyin Qin, Yansheng Wang, Xiaodong Ma, Luka Brcic, Mariano Provencio, Yangchao Chen, Chengzhi Zhou, Ming Liu
Summary: Ferroptosis-related genes can serve as prognostic markers for small cell lung cancer (SCLC) patients and are associated with tumor immune infiltration. The expression of TXNIP is highly correlated with immune infiltration and the efficacy of chemotherapy combined with immunotherapy in SCLC patients.
TRANSLATIONAL LUNG CANCER RESEARCH
(2022)
Article
Oncology
Philipp Jurmeister, Maximilian Leitheiser, Peggy Wolkenstein, Frederick Klauschen, David Capper, Luka Brcic
Summary: The combination of DNA methylation analysis and machine learning can reliably differentiate pleural mesothelioma (PM) from chronic pleuritis and lung cancer, including pleomorphic carcinomas. Additionally, the study identifies new candidate genes for PM carcinogenesis and demonstrates that deconvolution of DNA methylation data can provide insights into the composition of the tumor microenvironment.
Article
Pathology
Jan Hendrik Rueschoff, Martina Haberecker, Zoi Tsourti, Kristiaan Nackaerts, Marc de Perrot, Luka Brcic, Ernest Nadal, Sotirios Tsimpoukis, Steven G. Gray, Luca Ampollini, Joachim G. Aerts, Emanuela Felley-Bosco, Michaela B. Kirschner, Kim Monkhorst, Birgit Weynand, Fatemeh Bavaghar-Zaeimi, Miroslav Samarzija, Roger Llatjos, Stephen P. Finn, Enrico Silini, Jan von der Thusen, Nesa Marti, Karerina Vervita, Roswitha Kammler, Solange Peters, Rolf A. Stahel, Paul Baas, Isabelle Opitz
Summary: Pleural mesothelioma (PM) is a aggressive malignancy with poor prognosis. Low phosphorylated S6 (pS6) immunoreactivity is significantly correlated with longer progression-free survival (PFS) and overall survival (OS) in PM patients. In this study, high pS6 expression is associated with lower likelihood of complete resection and shorter OS and PFS in non-epithelioid PM patients.
Letter
Oncology
Markus G. Seidel, Luka Brcic, Gerald Hoefler, Caroline Hutter, Milen Minkov, Laura Sophie Steffen, Armin Zebisch, Martin Benesch
PEDIATRIC BLOOD & CANCER
(2023)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Birgit Pernthaler, Luka Brcic, Reingard Maria Aigner, Michael Fuchsjaeger, Emina Talakic
Summary: This article presents a rare case of primary malignant mesothelioma in the liver, with the patient being a 48-year-old woman who experienced increasing upper abdominal discomfort and inappetence, accompanied by a weight loss of approximately 10 kg. CT and MRI revealed a highly suspicious mass lesion in the liver. F-18-FDG PET/CT performed for staging showed a pathological F-18-FDG uptake of the known liver tumor. Histology and immunohistochemistry indicated mesothelioma of the liver.
CLINICAL NUCLEAR MEDICINE
(2023)
Article
Oncology
Abbas Agaimy, Luka Brcic, Laurence M. Briski, Yin P. Hung, Michael Michal, Michal Michal, G. Petur Nielsen, Robert Stoehr, Andrew E. Rosenberg
Summary: A focal adenomatoid-microcystic pattern is not uncommon in peritoneal mesothelioma, but tumors composed almost exclusively of this pattern are distinctly rare and have not been well characterized. Herein, we describe five peritoneal mesothelial neoplasms that are pure adenomatoid/microcystic in morphology and unified by the presence of an NR4A3 fusion. These tumors showed morphological similarities and lacked a conventional epithelioid or sarcomatoid component. At the last follow-up, all five patients were alive and disease-free.
GENES CHROMOSOMES & CANCER
(2023)
Article
Cell Biology
Stephan Ihrler, David Stiefel, Philipp Jurmeister, Ann Sandison, Nicola Chaston, Jan Laco, Nina Zidar, Luka Brcic, Robert Stoehr, Abbas Agaimy
Summary: The formal pathogenesis of salivary carcinosarcoma (SCS) is clarified in this study, showing that it almost always develops from pleomorphic adenoma (PA) and follows a multistep adenoma-carcinoma-sarcoma sequence. This finding suggests that SCS is a rare and unique variant of carcinoma ex PA with secondary sarcomatous overgrowth.
Article
Biochemistry & Molecular Biology
Sebastian P. Nischwitz, Julia Fink, Marlies Schellnegger, Hanna Luze, Vladimir Bubalo, Carolin Tetyczka, Eva Roblegg, Christian Holecek, Martin Zacharias, Lars-Peter Kamolz, Petra Kotzbeck
Summary: Persistent inflammation during wound healing is identified as a precipitating factor in the development of hypertrophic scars. However, lack of standardized models and limited evidence for therapeutic approaches hinder research progress. This study aimed to investigate scar formation mechanisms and develop a method for generating standardized hypertrophic scars through prolonged inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Arailym Sarsembayeva, Melanie Kienzl, Eva Gruden, Dusica Ristic, Kathrin Maitz, Paulina Valadez-Cosmes, Ana Santiso, Carina Hasenoehrl, Luka Brcic, Joerg Lindenmann, Julia Kargl, Rudolf Schicho
Summary: Cannabinoid (CB) receptors, particularly CB2, play an important role in the tumor microenvironment of non-small cell lung cancer (NSCLC). Deficiency of CB2 reduces tumor burden and enhances the activity of immune cells, leading to improved response to anti-PD-1 therapy. CB2 could be a potential adjuvant target for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sabrina Borchert, Alexander Mathilakathu, Alina Nath, Michael Wessolly, Elena Mairinger, Daniel Kreidt, Julia Steinborn, Robert F. H. Walter, Daniel C. Christoph, Jens Kollmeier, Jeremias Wohlschlaeger, Thomas Mairinger, Luka Brcic, Fabian D. Mairinger
Summary: The expression level of Fibroblast activation protein alpha (FAP) is associated with the formation of desmoplastic stromal reaction (DSR) in pleural mesothelioma (PM). DSR-high samples demonstrated a significant prolongation of median survival time, indicating the important role of cancer-associated fibroblasts (CAFs) in tumor prognosis. These findings provide a basis for introducing new therapeutic strategies and predictive biomarkers for survival in PM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tanja Grossmann, Andrijana Kirsch, Magdalena Grill, Barbara Steffan, Michael Karbiener, Luka Brcic, Barbara Darnhofer, Ruth Birner-Gruenberger, Markus Gugatschka
Summary: Research on human vocal fold biology is limited due to the sensitive microstructure of the vocal fold mucosa. We developed an alternative model using human buccal epithelial cells and vocal fold fibroblasts, which closely resembles native vocal fold mucosa in terms of histology and proteomics. This model provides a reproducible in vitro platform for various research opportunities.
Article
Respiratory System
Teresa Sassmann, Philipp Douschan, Vasile Foris, Natascha Troester, Katarina Zeder, Luka Brcic, Adrienn Tornyos, Gerhard Bachmaier, Michael Fuchsjaeger, Horst Olschewski, Gabor Kovacs
Summary: Even in the absence of significant pulmonary hypertension at rest, COPD patients show characteristic abnormalities in pulmonary hemodynamics during exercise, which may impact exercise capacity.
RESPIRATORY RESEARCH
(2022)
