Review
Biotechnology & Applied Microbiology
Wenwei Li, Zhen Zhou, Dan Wang
Summary: This paper summarizes the advantages and disadvantages of chemical hydrolysis, enzymatic biotransformation, and fermentation for the synthesis of L-cysteine. It provides a detailed introduction to the biosynthesis of L-cysteine, metabolic engineering strategies, and the latest progress in reported L-cysteine fermentation bacteria. Insights are given on the future development of increasing the production of biosynthetic L-cysteine.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Xiaomei Zhang, Zhenhang Sun, Jinyu Bian, Yujie Gao, Dong Zhang, Guoqiang Xu, Xiaojuan Zhang, Hui Li, Jinsong Shi, Zhenghong Xu
Summary: This study successfully increased the production efficiency of l-cysteine by optimizing serine production, constructing a biosensor, and implementing adaptive laboratory evolution. The engineered strain achieved efficient biosynthesis of l-cysteine from glycerol conversion.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Hai-De Wang, Jian-Zhong Xu, Wei-Guo Zhang
Summary: In this study, a high L-arginine (L-Arg) producing E. coli strain was obtained through metabolic engineering. The genes speC and speF were found to play important roles in L-Arg accumulation. Feedback inhibition of the L-Arg pathway in E. coli was successfully overcome by combining gene mutations/deletions. Additionally, the insertion of a gene cluster from Corynebacterium glutamicum enhanced the metabolic flux of the L-Arg pathway. The final strain achieved the highest reported level of L-Arg production by E. coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Yanan Hao, Xuewei Pan, Rufan Xing, Jiajia You, Mengkai Hu, Zhifei Liu, Xiangfei Li, Meijuan Xu, Zhiming Rao
Summary: By using a multi-modular strategy, a high-yielding L-valine-producing strain of Escherichia coli was obtained. Through modifications in the biosynthetic pathway, transport module, transcription factors, and NADPH supply, the strain achieved a high L-valine titer. This study is of significance for enhancing the production performance of L-valine and its derivatives in cell factories.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mengzhen Nie, Jingyu Wang, Kechun Zhang
Summary: A novel approach was developed to achieve high-yield production of L-arginine from N-acetylglutamate in E. coli by genetically modifying the bacteria and overexpressing specific genes. Co-production of pyruvate and L-arginine was also demonstrated to increase the utilization of input carbon sources.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Applied
Miaomiao Hu, Mengli Li, Chenchen Li, Tao Zhang
Summary: In this study, an efficient engineered strain for the biosynthesis of LNFP I was reported by rational design of metabolic pathways in Escherichia coli. The engineered strains produced high yields of LNFP I in fed-batch cultivation, with a conversion rate of lacto-N-tetraose to LNFP I reaching the highest level reported so far. The LNFP I-produced platform established here is broadly suitable for the production of complex and branched oligosaccharides.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Chemical
Xue Liu, Jincong Liu, Dengwei Lei, Guang-Rong Zhao
Summary: An artificial biosynthetic pathway of phlorizin was constructed in Escherichia coli, enabling the production of phloretic acid, phloretin, and phlorizin. Through modular engineering strategies and biochemical screening, successful biosynthesis of phloretin and phlorizin was achieved, demonstrating the potential of microbial cell factories in producing natural and unnatural dihydrochalcones.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li, Hong Xu
Summary: In this study, an ectoine-producing variant of E. coli was constructed using genetic manipulation and optimized for increased production. The highest ectoine titer produced by E. coli was achieved, showing great industrial prospects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sophia Tsouka, Meric Ataman, Tuure Hameri, Ljubisa Miskovic, Vassily Hatzimanikatis
Summary: The Network Response Analysis (NRA) is a novel framework for rational genetic strain design that integrates various analysis methods and constraints, providing a sophisticated alternative to traditional Metabolic Control Analysis (MCA) for metabolic engineering optimization. It allows for the incorporation of physiological data and the generation of multiple alternative optimal strategies given user-defined boundaries and objectives.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Mengmeng Cai, Zhenqiang Zhao, Xiangfei Li, Yuanyi Xu, Meijuan Xu, Zhiming Rao
Summary: In this study, a wild-type Escherichia coli was converted to a high-producer of L-homoserine using metabolic engineer strategies. By regulating the degradation pathway, optimizing copy numbers, and modifying the transport system, a final engineered strain was able to efficiently produce a high level of L-homoserine.
METABOLIC ENGINEERING
(2022)
Article
Agricultural Engineering
Yu Zhang, Minhua Wei, Guihong Zhao, Wenjie Zhang, Yingzi Li, Beibei Lin, Yanjun Li, Qingyang Xu, Ning Chen, Chenglin Zhang
Summary: In this study, a high-efficiency Escherichia coli strain capable of producing 60.1 g/L L-homoserine was constructed, achieving the highest fermentative production efficiency of L-homoserine to date. By dynamically attenuating the L-homoserine degradation pathway and employing systems metabolic engineering strategies, the production yield of L-homoserine was significantly increased. This engineered strain can be further applied for scale-up production of L-homoserine and its derivatives.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shuai Jiang, Ruirui Wang, Dehu Wang, Chunguang Zhao, Qian Ma, Heyun Wu, Xixian Xie
Summary: This study combined multilevel rational metabolic engineering with biosensor-assisted mutagenesis screening to exploit the L-arginine production potential of Escherichia coli. Multiple metabolic pathways were reprogrammed to redirect the metabolic flux into L-arginine synthesis, and a toggle switch was designed to dynamically control gene expression. A biosensor-assisted high-throughput screening platform was used to further explore L-arginine production potential. Through this approach, the engineered strain produced the highest values of L-arginine reported so far. The study demonstrated the effectiveness of rational metabolic reprogramming and biosensor-assisted mutagenesis screening in unleashing cellular potential for value-added metabolite production.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Zhenyu Zhang, Weike Su, Yunyun Bao, Qianqian Huang, Kai Ye, Pengfu Liu, Xiaohe Chu
Summary: This study optimized a microbial cell factory for efficient production of t4Hyp using a modular engineering approach. By reducing α-KG attrition, inhibiting L-proline consumption, and optimizing gene overexpression, promoter, copy number, and fusion system, a high yield of t4Hyp was achieved.
MICROBIAL CELL FACTORIES
(2022)
Article
Agriculture, Multidisciplinary
Lian Wang, Huijing Wang, Jianbin Chen, Minglong Hu, Xiaoyu Shan, Jingwen Zhou
Summary: A de novo biosynthesis pathway for chlorogenic acid was constructed in Escherichia coli via modular engineering, which increased metabolic flux and cofactor supply for efficient production of chlorogenic acid.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Meng Liu, Keqin He, Haoran Bi, Meng Wang, Biqiang Chen, Tainwei Tan, Yang Zhang
Summary: The biosynthesis pathway of 2-hydroxyadipate was constructed in Escherichia coli, and 7.11 g/L of 2-hydroxyadipate was produced in the 5 L bioreactor. By using potential optimization strategies through transcriptome analysis, 11.1 g/L of 2-hydroxyadipate was further achieved. These results provide a solid foundation for the biosynthesis of adipic acid and the production of bionylon.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qianwen Shi, Cheng Chen, Wei Zhang, Ping Wu, Meng Sun, Hui Wu, Haizhen Wu, Pengcheng Fu, Jianhua Fan
Summary: Eukaryotic microalgae are crucial primary producers in nature, with potential for bioactive substance production. Genetic engineering allows for gene editing and protein expression optimization in microalgae, expanding their applications in medicine and food industries.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Engineering, Civil
Hui Wu, Pengcheng Fu, Joseph P. Morris, Earl D. Mattson, Ghanashyam Neupane, Megan M. Smith, Adam J. Hawkins, Yuran Zhang, Timothy Kneafsey
Summary: This study demonstrates the feasibility of stochastic tracer modeling on a high-fidelity fracture network model, providing important insights into flow and transport characteristics in subsurface fractured reservoirs.
JOURNAL OF HYDROLOGY
(2021)
Article
Multidisciplinary Sciences
Ziyan Li, Derek Elsworth, Chaoyi Wang, L. Boyd, Z. Frone, E. Metcalfe, A. Nieto, S. Porse, W. Vandermeer, R. Podgorney, H. Huang, T. McLing, G. Neupane, A. Chakravarty, P. J. Cook, P. F. Dobson, C. A. Doughty, Y. Guglielmi, C. Hopp, M. Hu, R. S. Jayne, S. E. Johnson, K. Kim, T. Kneafsey, S. Nakagawa, G. Newman, P. Petrov, J. C. Primo, M. Robertson, V. Rodriguez-Tribaldos, J. Rutqvist, M. Schoenball, E. L. Sonnenthal, F. A. Soom, S. Sprinkle, C. Ulrich, C. A. Valladao, T. Wood, Y. Q. Zhang, Q. Zhou, L. Huang, Y. Chen, T. Chen, B. Chi, Z. Feng, L. P. Frash, K. Gao, E. Jafarov, S. Karra, N. Makedonska, D. J. Li Li, R. Pawar, N. Welch, P. Fu, R. J. Mellors, C. E. Morency, J. P. Morris, C. S. Sherman, M. M. Smith, D. Templeton, J. L. Wagoner, J. White, H. Wu, J. Moore, S. Brown, D. Crandall, P. Mackey, T. Paronish, S. Workman, B. Johnston, K. Beckers, J. Weers, Y. Polsky, M. Maceira, C. P. Chai, A. Bonneville, J. A. Burghardt, J. Horner, T. C. Johnson, H. Knox, J. Knox, B. Q. Roberts, P. Sprinkle, C. E. Strickland, J. N. Thomle, V. R. Vermeul, M. D. White, D. Blankenship, M. Ingraham, T. Myers, J. Pope, P. Schwering, A. Foris, D. K. King, J. Feldman, M. Lee, J. Su, T. Baumgartner, J. Heise, M. Horn, B. Pietzyk, D. Rynders, G. Vandine, D. Vardiman, T. Doe, J. McLennan, Y. S. Wu, J. Miskimins, P. Winterfeld, K. Kutun, M. D. Zoback, A. Singh, R. N. Horne, K. Li, A. Hawkins, Y. Zhang, E. Mattson, D. Elsworth, K. J. Im, Z. Li, C. J. Marone, E. C. Yildirim, J. Ajo-Franklin, A. Ghassemi, D. Kumar, V. Sesetty, A. Vachaparampil, H. F. Wang, H. Sone, K. Condon, B. Haimson, W. Roggenthen, C. Medler, N. Uzunlar, C. Reimers, M. W. McClure
Summary: This study emphasizes the importance of understanding the mechanisms controlling fluid injection-triggered seismicity in order to mitigate the impact of earthquakes. By conducting experiments and observations, researchers proposed a new framework to define maximum event magnitudes as a function of pre-existing critical stresses and fluid injection volume.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Geological
Hui Wu, Randolph R. Settgast, Pengcheng Fu, Joseph P. Morris
Summary: The study introduces an enhanced VCCT for calculating energy release rates and stress intensity factors for cracks with arbitrary shapes in 3D domains on structured grids. It was implemented into a multiphysics simulation environment capable of simulating crack propagation in the framework of linear elastic fracture mechanics. The enhanced VCCT was validated through comparisons with existing analytical/numerical solutions and other VCCT approaches for SIF calculation along nonsmoothed crack fronts.
ROCK MECHANICS AND ROCK ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Haitao Deng, Jingyi Li, Yao Zhou, Yang Xia, Chao Chen, Zhemin Zhou, Hui Wu, Ping Wang, Shengmin Zhou
Summary: NocPer is a highly sensitive ratiometric pH probe that is suitable for the physiological pH range, with a wide fluorescence peak range and dual excitation peaks for ratio imaging.
Article
Agricultural Engineering
Peng Fei, Yuanchan Luo, Ningyu Lai, Hui Wu
Summary: Acetate is a potential non-food carbon source for industrial production to address the shortage of food-based feedstocks. This study successfully constructed a biosynthesis pathway for R-3HB using engineered E. coli strains, which efficiently utilized syngas-derived acetate to synthesize R-3HB.
BIORESOURCE TECHNOLOGY
(2021)
Article
Geochemistry & Geophysics
Pengcheng Fu, Martin Schoenball, Jonathan B. Ajo-Franklin, Chengping Chai, Monica Maceira, Joseph P. Morris, Hui Wu, Hunter Knox, Paul C. Schwering, Mark D. White, Jeffrey A. Burghardt, Christopher E. Strickland, Timothy C. Johnson, Vince R. Vermeul, Parker Sprinkle, Benjamin Roberts, Craig Ulrich, Yves Guglielmi, Paul J. Cook, Patrick F. Dobson, Todd Wood, Luke P. Frash, Lianjie Huang, Mathew D. Ingraham, Joseph S. Pope, Megan M. Smith, Ghanashyam Neupane, Thomas W. Doe, William M. Roggenthen, Roland Horne, Ankush Singh, Mark D. Zoback, Herb Wang, Kate Condon, Ahmad Ghassemi, Hao Chen, Mark W. McClure, George Vandine, Douglas Blankenship, Timothy J. Kneafsey
Summary: This study analyzed data from hydraulic fracturing tests in an underground testbed to determine the characteristics and development process of fractures. It was found that hydraulic fractures could cross natural fractures and continue to propagate under continued stimulation, while mineral-filled natural fractures did not have a significant impact on hydraulic fracture propagation. The high-quality data sets allowed confident conclusions to be drawn, highlighting the advantages of intermediate-scale experiments in subsurface research.
JOURNAL OF GEOPHYSICAL RESEARCH-SOLID EARTH
(2021)
Article
Biotechnology & Applied Microbiology
Ningyu Lai, Yuanchan Luo, Peng Fei, Peng Hu, Hui Wu
Summary: The study demonstrates the biosynthesis of 3-hydroxypropionic acid (3-HP) in metabolically engineered Escherichia coli using acetic acid and CO2 as substrates, with promising results when using syngas-derived acetic acid as a replacement for pure acetic acid. This could potentially contribute to the future development of an industrial bioprocess for 3-HP production from syngas-derived acetic acid.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Ju Wu, Xiangju Wei, Pengye Guo, Aiyong He, Jiaxing Xu, Mingjie Jin, Yanjun Zhang, Hui Wu
Summary: This study investigated the effect of corn stover hydrolysate (CSH) as a carbon source on the biosynthesis of P(3HB-co-LA), finding that reducing the activity of the electron transport system led to a higher fraction of lactate (LA) in the copolymer, and replacing the promoter of the key gene pctth with ldhA gene promoter dynamically modulated the expression of pctth gene and changed the lactic acid content.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Pengye Guo, Yuanchan Luo, Ju Wu, Hui Wu
Summary: This article discusses the importance and application of polylactic acid and its copolymers in the field of renewable biological resources, explores the optimization of production strategies through copolymerization reactions and microbial metabolism processes, and the necessity of regulating LA monomer fraction.
BIORESOURCES AND BIOPROCESSING
(2021)