Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 23, Issue 2, Pages 150-157Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-018-0058-2
Keywords
curcumin; exosome (EXO); stability; fluorescence monitoring; reactive oxygen species (ROS); antioxidant
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Funding
- National Research Foundation (NRF) - Ministry of Education, Science and Technology [NRF- 2017R1A2B 4001964]
- Agri-Bio Industry Technology Development Program, Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET), Republic of Korea [316028-3]
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In this study, we compared the antioxidant activities of curcumin (Cur) and a Cur formulation using a fluorescence analysis assay. The Cur formulation was prepared by a simple incorporation of Cur into exosomes (EXO) to produce Cur/EXOs. Free Cur had a low fluorescence intensity in aqueous solution because of its poor stability as a result of its autoxidation, whereas a significantly higher fluorescence intensity was observed for Cur/EXOs. Compared to free Cur, the increased level of intact Cur in EXOs allowed for enhanced antioxidant activity in H2O2 scavenging activity and DPPH assays. Compared to Cur at high concentration (200 mu M), Cur/ EXOs were significantly less cytotoxic. The antioxidant activity of Cur or Cur/EXOs in cells could be easily demonstrated by monitoring decreases in their fluorescence intensity. Following subcutaneous injection, the fluorescence intensities of Cur/EXOs were much higher than that of Cur, suggesting that Cur/EXOs improve Cur stability in vivo. Taken together, we have demonstrated the superiority of Cur/EXOs over free Cur in terms of aqueous stability and antioxidant activity using fluorescence monitoring both in vitro and in vivo.
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