Review
Microbiology
Manon Molina, Gianluca Cioci, Claire Moulis, Etienne Severac, Magali Remaud-Simeon
Summary: Glucansucrases and branching sucrases, produced by lactic acid bacteria, are enzymes involved in synthesizing alpha-glucans from sucrose. They contribute to cell protection and facilitate adhesion and colonization in various biotopes. Research on their molecular mechanisms has led to the discovery of new catalysts for transforming sucrose into value-added molecules.
Article
Food Science & Technology
Jung-Hyun Ju, Sang-Gyu Jeon, Sun-Yeon Heo, Ji-Soo Kim, Min-Ho Jo, Min-Soo Kim, Chul-Ho Kim, Baek-Rock Oh
Summary: In this study, a novel strain of Lactobacillus reuteri BR120 that produces abundant exopolysaccharides (EPSs) with an alternan-type structure was isolated. The gamma-ray mutagenesis of Lactobacillus reuteri BR120 resulted in L. reuteri EC01, which exhibited a 1.3-fold increased production of alternan-type compound. L. reuteri EC01 showed various probiotic activities and produced 54.3 g/L of the alternan-type EPS through fed-batch fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Liansheng Yu, Zhigang Qian, Jingping Ge, Renpeng Du
Summary: Glucansucrase (GS) is an important enzymatic tool that has great potential in various fields. Different strains produce different GSs, which can synthesize exopolysaccharides (EPSs) with different glycosidic bond structures and biological functions. This review summarizes the isolation, purification, properties, detection methods, sources, and applications of GS, providing a reference for its research and development.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Physical
Reinaldo Fraga Vidal, Roberto Carlos Aristicas Ribalta, Lisandra Teresa Martinez Valdes, Meinardo Lafargue Gamez, Amanda Montes Alvarez, Arianne Rubio Sanchez, Eric Dubreucq, Benoit Moreau
Summary: The study showed that hybrids and chimeric biocatalyst DSases with novel binding capacity to cellulose can be designed to purify and immobilize using renewable lignocellulosic materials as supports. By designing a novel DSR-F- increment SP- increment GBD-CBM2a chimeric dextransucrase variant, this goal was successfully achieved.
Article
Agriculture, Multidisciplinary
Tjaard Pijning, Joana Gangoiti, Evelien M. te Poele, Tim Boerner, Lubbert Dijkhuizen
Summary: This study presents the crystal structures of two GtfB enzymes and their homology models, demonstrating their ability to convert both linear and branched starch substrates. Compared to previously described GtfBs, these enzymes feature a much more open binding groove.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Evelien M. te Poele, Stevan E. van der Hoek, Anastasia C. Chatziioannou, Gerrit J. Gerwig, Wouter J. Duisterwinkel, Lizette A. A. C. M. Oudhuis, Joana Gangoiti, Lubbert Dijkhuizen, Hans Leemhuis
Summary: The study reports a novel thermostable starch-acting enzyme Geobacillus sp. 12AMOR1 GtfC, which has the ability to synthesize starch at high temperatures and shows anti-staling effects in bread bakery products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yuqi Yang, Xinqi Zhao, Tao Zhang, Bruce R. Hamaker, Ming Miao
Summary: In this study, a glucanotransferase from prokaryotic Azotobacter chroococcum was recombinantly expressed and found to have high affinity for high-amylose starch. The enzyme can convert starch into small polymer molecules with narrow size distribution, leading to the synthesis of a promising prebiotic dietary fiber.
Review
Food Science & Technology
Jinsong Wu, Xiangpeng Han, Meizhi Ye, Yao Li, Xi Wang, Qingping Zhong
Summary: This review provides a detailed summary of the biosynthesis pathway, production parameter, and structure-function relationship of LAB-derived EPS. It also discusses the structural modifications and the current and potential applications of LAB-derived EPS.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Microbiology
Minhui Kim, Jae-Kweon Jang, Young-Seo Park
Summary: Leuconostoc lactis SBC001 can produce glucansucrase and synthesize oligosaccharides, which have prebiotic effects on bacterial and yeast strains, and anti-inflammatory activity in RAW 264.7 macrophage cells. The optimal conditions for oligosaccharide production were identified, and the structure of the oligosaccharides was analyzed using H-1-NMR spectroscopy.
Article
Nutrition & Dietetics
Wiebke Schloermann, Julia A. Bockwoldt, Sabine M. Huebner, Elisa Wittwer, Sarah Reiners, Stefan Lorkowski, Christine Dawczynski, Matthias A. Ehrmann, Michael Glei
Summary: The aim of this study was to investigate the production of beta-glucan and the potential prebiotic and chemopreventive effects of wheat and rye sourdoughs and breads. The effects of wild-type and non-beta-glucan-forming mutant strains of Levilactobacillus brevis and Pediococcus claussenii were examined. The results showed that concentrations of short-chain fatty acids (SCFA) increased and ammonia concentrations decreased in the fermented samples. Treatment with fermentation supernatants (FS) reduced the growth and viability of LT97 colon adenoma cells and increased caspase-2 and -3 activities. The expression levels of several genes and proteins were also affected.
Article
Nutrition & Dietetics
V. B. Chandana Kumari, Sujay S. Huligere, Ghallab Alotaibi, Abdulaziz K. Al Mouslem, Ammar Abdulraheem Bahauddin, Thippeswamy Boreddy Shivanandappa, Ramith Ramu
Summary: Probiotic strains from fermented sugarcane juice, particularly RAMULAB54, showed strong inhibitory effect on α-glucosidase and α-amylase activities. The cell-free supernatant (CS) of RAMULAB54, rich in lactic acid, citric acid, and hydroxycitric acid, exhibited the highest inhibition. These findings suggest the potential use of these isolates in managing postprandial hyperglycemia and improving intestinal health.
Article
Engineering, Chemical
Yiya Wang, Tao Sun, Yinzhu Wang, Hao Wu, Yan Fang, Jiangfeng Ma, Min Jiang
Summary: In this study, Leuconostoc pseudomesenteroides G29 was found to produce two types of exopolysaccharides from sucrose, soluble and insoluble alpha-glucans. The production of soluble alpha-glucan was increased by regulating pH, leading to potential applications of the insoluble alpha-1,3-glucan as biomaterials and/or biochemicals. Analysis of the structures of the exopolysaccharides provides insights into their production by L. pseudomesenteroides.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Review
Agriculture, Dairy & Animal Science
Shuo Yang, Xiaoqing Xu, Qing Peng, Lan Ma, Yu Qiao, Bo Shi
Summary: The over-use or misuse of antibiotics in livestock and poultry production has led to a rising threat of antibiotic resistance in animals and negative ecological effects. Exopolysaccharides from lactic acid bacteria (LAB-EPS) have been found to have anti-inflammatory and antimicrobial activities, and can regulate intestinal health and the immune system in livestock. LAB-EPS are considered as ideal alternatives to antibiotics due to their biodegradability, non-toxicity, and bio-compatibility.
Review
Biotechnology & Applied Microbiology
Ziwei Chen, Dawei Ni, Wenli Zhang, Timo Stressler, Wanmeng Mu
Summary: Lactic acid bacteria have the capability to produce various exopolysaccharide alpha-glucans, which have vast commercial and application potential in various fields. The use of GH70 family enzymes in the biosynthesis of alpha-glucans allows for efficient production with different structures, and research has focused on biochemical characterization and future applications of LAB-derived alpha-glucans.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Microbiology
Sujay S. Huligere, V. B. Chandana Kumari, Sudhanva M. Desai, Ling Shing Wong, Nagma Firdose, Ramith Ramu
Summary: This study evaluates the probiotic and antidiabetic properties of lactic acid bacteria (LAB) obtained from milk and dairy-based products. The strains were tested for their physiological, biochemical, and molecular characteristics. The results show that these LAB isolates inhibit carbohydrate hydrolysis enzymes, have antioxidant activity, exhibit high survival rate under gastrointestinal conditions, and show resistance to antibiotics. They also demonstrate good attachment properties and potential application in various sectors. Further in vivo testing is needed to confirm their probiotic potential.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Alwin M. Hartman, Varsha R. Jumde, Walid A. M. Elgaher, Evelien M. Te Poele, Lubbert Dijkhuizen, Anna K. H. Hirsch
Summary: The study introduces the application of DCC to discover novel ligands of bacterial virulence factor GTF180, leading to the synthesis and analysis of a series of glucose and maltose-based acylhydrazones. The results reveal the potential mechanism of four compounds and their binding affinity, highlighting the potential for further development as inhibitors.
Article
Agriculture, Dairy & Animal Science
Rivca L. Valk-Weeber, Kelly Nichols, Lubbert Dijkhuizen, Etske Bijl, Sander S. van Leeuwen
Summary: Bovine milk serum whey proteins such as lactoferrin, IgG, and GlyCAM-1 contribute significantly to N-glycan content. Variation in glycan monosaccharide composition can impact functionality, with individual cows showing differences in glycosylation profiles. Dietary supplementation has limited influence on milk serum glycoprofile, while variations in fucosylation levels of GlyCAM-1 are more related to individual cow differences than nutrient supply.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Evelien M. te Poele, Stevan E. van der Hoek, Anastasia C. Chatziioannou, Gerrit J. Gerwig, Wouter J. Duisterwinkel, Lizette A. A. C. M. Oudhuis, Joana Gangoiti, Lubbert Dijkhuizen, Hans Leemhuis
Summary: The study reports a novel thermostable starch-acting enzyme Geobacillus sp. 12AMOR1 GtfC, which has the ability to synthesize starch at high temperatures and shows anti-staling effects in bread bakery products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Food Science & Technology
Xiaodan Li, Xiangfeng Meng, Tijn C. De Leeuw, Evelien M. te Poele, Tjaard Pijning, Lubbert Dijkhuizen, Weifeng Liu
Summary: This paper reviews the research on the glucosylation of polyphenols using GH70 glucansucrase and branching sucrases. The efficient catalysis, regioselectivity of glucosylation, and structure of the generated products are described for these enzymes. Enzyme engineering is effective for improving their catalytic efficiency and product specificity.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Multidisciplinary
Yawen Jiang, Xiaoxiao Li, Tjaard Pijning, Yuxiang Bai, Lubbert Dijkhuizen
Summary: This study elucidated the crystal structure of Lr121 GtfB 4,6-alpha-GTase and identified structural features that determine its reaction and product specificity through amino acid residue alignments. Mutants S1057P and Q1126I showed improved catalytic efficiencies. Changes in the ratio of (alpha 1 -> 6) over (alpha 1 -> 4) linkages in maltodextrin-derived products were observed in mutants H1056G and Q1126I.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Tjaard Pijning, Joana Gangoiti, Evelien M. te Poele, Tim Boerner, Lubbert Dijkhuizen
Summary: This study presents the crystal structures of two GtfB enzymes and their homology models, demonstrating their ability to convert both linear and branched starch substrates. Compared to previously described GtfBs, these enzymes feature a much more open binding groove.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Wei Xu, Dawei Ni, Xiaodong Hou, Tjaard Pijning, Albert Guskov, Yijian Rao, Wanmeng Mu
Summary: This study reported the crystal structures of LS from the G(-)-bacterium Brenneria sp. EniD 312, as well as two mutants (A154S, H327A) targeting positions known to affect LS reaction specificity. The H327A mutant resulted in smaller products synthesized, while the A154S mutation affected the enzyme's affinity for sucrose, making it less effective in transfructosylation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Xiangfeng Meng, Xiaodan Li, Tjaard Pijning, Xiaofei Wang, Sander S. van Leeuwen, Lubbert Dijkhuizen, Guanjun Chen, Weifeng Liu
Summary: This study revealed the potential of GH70 branching sucrases for glucosylating noncarbohydrate molecules. The (alpha 1 -> 3) branching sucrase GtfZ-CD2 was found to glucosylate benzenediol compounds and showed a preference for adjacent aromatic hydroxyl groups. Semirational engineering of GtfZ-CD2 variants improved the glucosylation efficiency of resorcinol and hydroquinone. This research provides insight into the mechanism of acceptor substrates binding and demonstrates the potential of using branching sucrase as an effective enzymatic glucosylation tool.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Xiaoxiao Li, Yu Wang, Jing Wu, Zhengyu Jin, Lubbert Dijkhuizen, Maher Abou Hachem, Yuxiang Bai
Summary: In this study, five different starches were modified using enyzmes, and the thermo-reversibility of the modified starch gels was evaluated. The results showed that modified potato starch exhibited thermo-reversibility and improved stability. This suggests that modified potato starch has the potential to be used as a substitute for kappa-carrageenan and other hydrocolloids in food applications.
FOOD HYDROCOLLOIDS
(2023)
Article
Agriculture, Multidisciplinary
Tjaard Pijning, Evelien M. te Poele, Tijn C. de Leeuw, Albert Guskov, Lubbert Dijkhuizen
Summary: GtfC-type 4,6-alpha-glucanotransferase (alpha-GT) enzymes can modify starch into low-glycemic index food ingredients. Despite differences with the related GtfB-type enzymes, GtfCs exhibit high structural similarity in the core domains and have a similar tunneled active site.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Food Science & Technology
Xiaoxiao Li, Yu Wang, Jing Wu, Zhengyu Jin, Lubbert Dijkhuizen, Birte Svensson, Yuxiang Bai
Summary: The modification of starch by transglycosylases from glycoside hydrolase families has received significant attention as these enzymes can produce starch derivatives with novel properties. This review discusses the effects of variations in glycosidic linkage composition on the structure and properties of modified starches, as well as the potential processability and functionality of starch derivatives with different linkage combinations and structures.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Yu Fang, Meihong Dong, Sander S. van Leeuwen, Lubbert Dijkhuizen, Xiangfeng Meng, Weifeng Liu
Summary: This study explored two α-glucosidases (MT31a1 and MT31a2) from Myceliophthora thermophila and characterized their biochemical properties. MT31a1 displayed high thermostability and was able to synthesize main functional IMOs components from maltose. MT31a2, on the other hand, preferred substrates with α-(1?3) and α-(1?4) linkages. Both enzymes show potential for the synthesis of prebiotic glucooligosaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Justyna M. Dobruchowska, Bryndis Bjornsdottir, Olafur H. Fridjonsson, Josef Altenbuchner, Hildegard Watzlawick, Gerrit J. Gerwig, Lubbert Dijkhuizen, Johannis P. Kamerling, Gudmundur O. Hreggvidsson
Summary: Alginate lyases are enzymes that catalyze the depolymerization of alginate, a linear polysaccharide. In this study, two alginate lyase genes were cloned and expressed from a marine thermophilic bacterium. The recombinant enzymes showed high thermostability and activity at elevated temperatures. The NMR analysis revealed that one of the enzymes is an endolytic lyase, while the other is an exolytic lyase.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Zhi-Min Zhao, Zhi-Hua Liu, Tongtong Zhang, Rongqian Meng, Zhiqun Gong, Yibing Li, Jing Hu, Arthur J. Ragauskas, Bing-Zhi Li, Ying-Jin Yuan
Summary: Bioconversion of bioresources/wastes using Rhodococcus is a promising approach for producing lipid-derived bioproducts. However, bioconversion involves living cells and is a complex system that requires further understanding. This review highlights the potential of Rhodococcus for producing lipids from various wastes and presents genetic modifications using emerging tools for promoting bioconversion. Additionally, strategies for enhancing the bioaccessibility of lignin and its derived aromatics and synthesizing biodiesel and aviation fuels from lignin and lipids are discussed. The synergistic modification of microbial strains, substrates, and fermentation processes is proposed for sustainable biological lignin valorization.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Maddy L. Newby, Joel D. Allen, Max Crispin
Summary: A key aspect of successful viral vaccine design is the elicitation of neutralizing antibodies targeting viral attachment and fusion glycoproteins. Glycosylation can influence the antigenicity and immunogenicity of vaccines, with native-like glycosylation potentially enhancing immunogenicity. Understanding viral glycosylation can guide the development of future vaccines in various enveloped viruses.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Uganeeswary Suparmaniam, Man Kee Lam, Jun Wei Lim, Inn Shi Tan, Bridgid Lai Fui Chin, Siew Hoong Shuit, Steven Lim, Yean Ling Pang, Peck Loo Kiew
Summary: Microalgae have the ability to synthesize high-value phytochemicals, and subjecting them to abiotic stress can enhance the accumulation of valuable metabolites within their cells. This study explores the effects of manipulating abiotic stress on microalgae yields and discusses approaches to increase HVPCs production using abiotic stress.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Pei Zhou, Cong Gao, Wei Song, Wanqing Wei, Jing Wu, Liming Liu, Xiulai Chen
Summary: The engineering status of protein is crucial for the efficient bioproduction in microbial cell factories. Strategies such as protein engineering, protein modification, and protein assembly can effectively modify the protein status and enhance production efficiency.
BIOTECHNOLOGY ADVANCES
(2024)