Journal
BIOSENSORS & BIOELECTRONICS
Volume 107, Issue -, Pages 118-122Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.02.019
Keywords
Heteroassembled AuNPs; Sandwich-immunoassay; LSPR chip format; Hepatitis B virus; HBsAg
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Funding
- National Research Foundation of Korea (NRF) - Korea government [2017M2A2A6A01020938]
- Ministry of Food and Drug Safety [17162MFDS065]
- Inha University WCSL research grant [17162MFDS065]
- Ministry of Food & Drug Safety (MFDS), Republic of Korea [DY0002256438-17162미래사065-3] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2017M2A2A6A01020938, 22A20153613139] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study aimed to develop a more sensitive method for the detection of hepatitis B surface antigen (HBsAg) using heteroassembled gold nanoparticles (AuNPs). A single layered localized surface plasmon resonance (LSPR) chip format was developed with antigen-antibody reaction-based detection symmetry using AuNPs, which detected HBsAg at 10pg/mL. To further improve the detection limit, a modified detection format was fabricated by. fixing a secondary antibody (to form a heteroassembled sandwich format) to the AuNP monolayer, which enhanced the detection sensitivity by about 100, times. The developed heteroassembled AuNPs sandwich-immunoassay LSPR chip format was able to detect as little as 100 fg/mL of HBsAg within 10-15 min. In addition, the heteroassembled AuNPs sandwich-immunassay LSPR chip format did not show any non-specific binding to other tested antigens, including alpha fetoprotein (AFP), C-reactive protein (CRP), and prostate-specific antigen (PSA). These findings confirm that the proposed detection strategy of heteroassembled AuNPs sandwich-immunoassay LSPR chip format may provide a new platform for early diagnosis of various human diseases.
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