Review
Chemistry, Analytical
Cristian Ravariu
Summary: Neurotransmitters, such as dopamine, have been extensively studied for their detection using biosensors. Biosensors offer cheaper and more sensitive detection of dopamine compared to conventional methods. The integration of active biological materials with electronic devices has contributed to the development of more advanced biosensors.
Article
Materials Science, Multidisciplinary
Kan Tang, Wujian Miao, Song Guo
Summary: The study found that increasing the off state gate voltage increases the injection of Na+ ions, leading to drain current decay in OECT transfer characteristics; Crosslinking with PEDOT:PSS can significantly reduce the decay of drain current, improving the stability of OECT transfer characteristics; The decay of drain current is mainly attributed to the repetitive injection-extraction of cations under cycling gate bias.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biophysics
Felix Hempel, Jessica Ka Yan Law, Thanh Chien Nguyen, Ruben Lanche, Anna Susloparova, Xuan Thang Vu, Sven Ingebrandt
Summary: The study investigated single-cell adhesion on organic electrochemical transistors using an impedimetric readout method, demonstrating single-cell resolution and potential future applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Renan Colucci, Bianca de Andrade Feitosa, Gregorio Couto Faria
Summary: This study evaluated the impact of different cation counterparts on the performance of organic electrochemical transistors (OECTs). The results showed that cations with a higher ability of salting-in effectively swell the channel, while cations with a higher ability to salt-out promote smaller modulations in the channel's current.
ADVANCED ELECTRONIC MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Yanping Chen, Jie Meng, Yiman Xu, Yaogang Li, Qinghong Zhang, Chengyi Hou, Hengda Sun, Gang Wang, Hongzhi Wang
Summary: In this study, an integrated ionic-additive assisted wet-spinning strategy was used to improve the conductivity and stretchability of PEDOT:PSS fiber, resulting in a fiber with extremely high conductivity and 30% stretchability. Additionally, an all-solid-state fibrous organic electrochemical transistor (OECT) device with a transconductance of 2.44 mS was fabricated based on this fiber.
ADVANCED ELECTRONIC MATERIALS
(2021)
Article
Engineering, Environmental
Keren Chen, Longjiao Zhu, Kunlun Huang, Wenqiang Zhang, Wentao Xu
Summary: In this study, a docking-aided rational tailoring strategy was established to refine the malachite green DNA aptamers, and a label-free ratiometric fluorescent aptasensor was developed for the detection of environmentally hazardous bio-molecules. The aptasensor demonstrated outstanding detection performance with high sensitivity and low limit of detection.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemical Research Methods
Dan Zheng, Jingyun Zhang, Wenxin Jiang, Ying Xu, Haixu Meng, Chueh Loo Poh, Chia-Hung Chen
Summary: A novel droplet modifiable graphene oxide (GO) aptasensor has been developed for sensitive flexible detection of different target compounds secreted from single cells. The sensor has low-background detection and high signal-to-noise ratio, and the aptamer sequences can be modified to measure different metabolic secretions. In application, the sensor was used to precisely measure the compounds secreted by engineered cells for bioproduction, and differentiate secretory cells from wild-type cells.
Article
Chemistry, Multidisciplinary
Tamsyn Stanborough, Fiona M. Given, Barbara Koch, Campbell R. Sheen, Andre Buzas Stowers-Hull, Mark R. Waterland, Deborah L. Crittenden
Summary: The emergence of SARS-CoV-2 in late 2019 led to a global pandemic in 2020, highlighting the need for more effective ways of detecting viral infections. This study investigated three optical techniques for quantifying the binding of recombinant SARS-CoV-2 spike protein to surface-immobilized oligonucleotide aptamers, with surface-enhanced Raman spectroscopy showing the highest sensitivity for detecting spike protein. These results demonstrate the potential of SERS-based aptasensors for rapid, point-of-use antigen detection systems.
Article
Engineering, Biomedical
Congran Jin, Ziqian Wu, John H. Molinski, Junhu Zhou, Yundong Ren, John X. J. Zhang
Summary: Advancement in material science, particularly in the development of nanomaterials, has led to the creation of plasmonic biosensors that enable real-time diagnosis of various diseases through biomarker screening. These versatile and portable devices can be used in point-of-care settings, allowing for convenient and efficient testing of biomarkers in homes or elsewhere. This article reviews the recent progress in the design of these biosensors, including the development of nanomaterials and the applications in point-of-care diagnosis. Additionally, challenges and opportunities, such as incorporating machine learning algorithms and the testing of Covid-19, are discussed.
MATERIALS TODAY BIO
(2022)
Article
Biophysics
Maui Nishio, Kaori Tsukakoshi, Kazunori Ikebukuro
Summary: G-quadruplex (G4) is a non-canonical structure formed in G-rich sequences of nucleic acids, playing important roles in vivo and having potential applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Priya Vizzini, Elena Beltrame, Nicola Coppede, Filippo Vurro, Francesco Andreatta, Emanuela Torelli, Marisa Manzano
Summary: This study proposes a textile organic electrochemical transistor-based (OECT) biosensor for detecting L. monocytogenes in food samples. An organic channel doped with PEDOT:PSS is used in the biosensor. AFM is used to obtain topographic maps of the gold gate surface, and the electrochemical activity on the gate electrodes is measured to determine the concentration of DNA and the presence of L. monocytogenes. The assay has a detection limit of 1.05 ng/μL and allows for the rapid and specific detection of L. monocytogenes in food samples.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Miaoron Zhamg, Yan Zhang, Chuankai Yang, Chunyun Ma, Yuhang Zhang, Jianguo Tang
Summary: The novel colorimetric biosensor based on La-MFs fabricated via biomineralization showed enhanced detection performance for EP, with a wide linear detection range and good selectivity. The La-MFs exhibited satisfactory storage stability and reusability for EP detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Physical
Ruijin Zeng, Weijun Wang, Mingming Chen, Qing Wan, Caicheng Wang, Dietmar Knopp, Dianping Tang
Summary: This study introduces a CRISPR-Cas12a-based instrument-free point-of-care detection technology, which uses smartphone for visualized DNA detection results, and combines wireless sensing technology, significantly improving portability and detection sensitivity.
Article
Biophysics
Yunjia Song, Hao Zhang, Tushita Mukhopadhyaya, Anthony Shoji Hall, Howard E. Katz
Summary: The new measurement configurations based on organic electrochemical transistors (OECTs) have shown improved sensitivity and precision in detecting antibody-antigen interactions. By utilizing specific bioreceptor layers and dual gate functionalization, drift effects are minimized, leading to more accurate results.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Multidisciplinary
Chao Wang, Mei Liu, Zhifei Wang, Song Li, Yan Deng, Nongyue He
Summary: The COVID-19 pandemic has highlighted the importance of developing point-of-care (POC) diagnostics, which offer advantages such as faster diagnostic speed, lower cost, and higher efficiency. Development of POC detection methods and devices using microfluidics, MEMS technology, nanotechnology and materials science is key to providing timely prevention and control of infectious diseases.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)