Article
Immunology
Geraldo Balieiro Neto, Jair Rodini Engracia Filho, Fabio Enrique Lemos Budino, Acyr Wanderley de Paula Freitas, Weber Vilas Boas Soares
Summary: The use of antimicrobial growth promoters is banned due to drug residues and increased bacterial resistance. Immunizing chickens with specific antigens to generate antibodies can be an alternative to antibiotics. However, protein impurities and biotin concentrations in IgY antibody samples may affect the accuracy of diagnostic tests.
Article
Chemistry, Analytical
Dandan Song, Jing Zheng, Nosang Myung, Jingli Xu, Min Zhang
Summary: A sandwich-type electrochemical immunosensor was developed for the detection of CEA, with excellent sensing performance and low detection limit. The magnetic assembly of nanocomposites simplifies further modification process, improving the reliability and reproducibility of the sensor.
Article
Chemistry, Analytical
Yarong Ren, Juan Wei, Ye Wang, Peng Wang, Yanwei Ji, Baoyuan Liu, Jianlong Wang, Gualberto Gonzalez-Sapienza, Yanru Wang
Summary: In this study, a broad-spectrum Salmonella nanobody-01 (Nb-01) was isolated and applied in the development of a streptavidin-bridged sandwich ELISA (SAB-ELISA) for simultaneously identifying five Salmonella serovars. The SAB-ELISA showed high sensitivity and detection limits, and demonstrated excellent feasibility and precision in actual samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Fanfan Yang, Long Xu, Alberto C. P. Dias, Xiaoying Zhang
Summary: The competitive sandwich immunoassay offers a sensitive and accurate method for detecting histamine in seafood, with a wide linear range and low detection limit.
Article
Chemistry, Analytical
Haohua Yang, Xueqi Zhao, Ziwei Zhang, Pinyi Ma, Xinghua Wang, Daqian Song, Ying Sun
Summary: This paper presents a sensitive SPR biosensor based on ZnO@Au nanomaterial for the detection of hIgG. The biosensor utilizes a sandwich strategy using biotin-streptavidin for secondary signal amplification. With optimal experimental conditions, the biosensor achieves a favorable concentration range of 0.0375-40 μg/mL for hIgG determination, which is approximately 67 times lower than conventional gold film-based SPR sensors.
Article
Chemistry, Physical
Andreea Campu, Frederic Lerouge, Dana Maniu, Klara Magyari, Monica Focsan
Summary: The study proposes the use of Surface Enhanced Infrared Absorption technology for efficient, specific, and ultrasensitive detection of biotin-streptavidin recognition interaction using a gold nano-bipyramid-based immunosensor. Successful grafting of p-ATP@biotin onto the gold surface and successful capture of the target streptavidin protein were confirmed through FT-IR experimental measurements and theoretical simulations. The development shows promise for efficient ultrasensitive SEIRA biosensing devices for more complex biologically relevant applications.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Biophysics
Asta Kausaite-Minkstimiene, Anton Popov, Ursule Kalvaityte, Eiva Bernotiene, Ali Mobasheri, Almira Ramanaviciene
Summary: This paper describes the development of a novel surface plasmon resonance (SPR) immunosensor for highly sensitive quantitative determination of human articular cartilage oligomeric matrix protein (COMP). The immunosensor uses a dual sandwich-type signal amplification strategy to achieve a drastic increase in sensitivity and has a wide detection range from 2.80 to 680.54 fM. It shows good repeatability, reproducibility, and operational stability, and exhibits high accuracy in analyzing COMP derived from articular cartilage in clinical studies.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Jingxian Li, Hongfen Yang, Ren Cai, Weihong Tan
Summary: A novel sandwich-type immunosensor was developed using Pt@ SnS2 as a matrix and N,B-Eu MOFs as a signal amplifier. The immunosensor showed high sensitivity and selectivity for the detection of CEA. It exhibited excellent ECL performance and stability, making it suitable for real human serum.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Lingli Qu, Mengdie Wu, Lu Zhao, Jiang Li, Hongzhi Pan
Summary: The detection signal of CA125 can be amplified via the dual MOF sandwich strategy. A versatile method for synthesizing uniform MXene and MIL-101(Fe)-NH2 composites is proposed, combining the advantages of both materials to build a base layer with excellent performance. MXene has excellent electrical conductivity and high surface area, while MIL-101(Fe)-NH2 increases the loading capacity of the primary antibody and possesses catalytic activity. UiO66@MB is fabricated as an electrochemical immunosensor signal tag to enable the detection of CA125. The proposed immunosensor demonstrates high sensitivity with a low LOD of 0.006 U/mL, providing a novel method for the early diagnosis of CA125.
Article
Chemistry, Analytical
Qian Cheng, Jinhui Feng, Tingting Wu, Yujian Guo, Xu Sun, Xiang Ren, Jin Yong Lee, Lei Liu, Qin Wei
Summary: A novel signal-off type photoelectrochemical (PEC) immunosensor was developed using Bi2S3/I:BiOCl and Au NPs@CoSnO3 as materials for sensitive detection of CYFRA 21-1. The heterostructure was formed through a simple, fast, low temperature production process with no complex raw materials, achieving a low detection limit and displaying good specificity and stability.
Article
Chemistry, Physical
Soliman Gamal, Doaa A. Kospa, Ahmed Gebreil, S. A. El-Hakam, Awad I. Ahmed, Amr Awad Ibrahim
Summary: In order to achieve large-scale commercial applications of fuel cells, the development of environmentally friendly, cost-effective, and highly durable metal-free carbon-based electrocatalysts for the cathodic ORR is required. Researchers developed TiO2 nanoparticles supported by a nitrogen-doped carbon matrix derived from the pyrolysis of NH2-MIL-125 (MOF), with controllable morphology, porosity, and particle size achieved through the introduction of a SiO2 template. The synergic covalent coupling between metal oxide spinel (AB2O4, particularly NiCo2O4) and TiO2/N-doped nanocarbon effectively enhanced the ORR catalytic activity.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Chemistry, Physical
Jing-Xian Zhang, Chang Tang, Di -Nan Chen, Lu-Yao Jiang, Ai-Jun Wang, Jiu-Ju Feng
Summary: A new sandwich-typed electrochemical immunosensor was developed for ultrasensitive detection of cardiac biomarker myoglobin. This biosensor exhibited a wide linear dynamic range, low detection limit, and reliable results in human serum samples.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Analytical
Luchen Ai, Yue Wang, Yunlei Zhou, Huanshun Yin
Summary: The novel and selective photoelectrochemical immunosensor for m6A detection was successfully prepared through in-situ production of an electron donor strategy. It showed a wide detection range and low detection limit, as well as good selectivity, reproducibility, and stability. Additionally, it was applicable for studying the m6A content in different parts of rice seedlings.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Electrochemistry
Aiping Wang, Yuya Li, Xiaojuan You, Shoutao Zhang, Jingming Zhou, Hongliang Liu, Peiyang Ding, Yumei Chen, Yanhua Qi, Yankai Liu, Chao Liang, Xifang Zhu, Ying Zhang, Enping Liu, Gaiping Zhang
Summary: An electrode platform immunosensor was developed for efficient and sensitive detection of SARS-CoV-2. The method showed high sensitivity, stability, and specificity, and has the potential to be used as a practical detection method for SARS-CoV-2.
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Jing-Xian Zhang, Chun-Lin Lv, Chang Tang, Lu-Yao Jiang, Ai-Jun Wang, Jiu-Ju Feng
Summary: In this study, a sandwich-type electrochemical immunosensor was constructed using PtCo highly branched nanocrystals as electrode substrates and dendritic mesoporous SiO2@AuPt nanoparticles as signal labels for ultrasensitive detection of SCCA. The sensor exhibited a broader linear range and lower detection limit, along with good reproducibility and recovery in diluted human serum samples.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)