Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 82, Issue 7, Pages 1081-1089Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2018.1459175
Keywords
microRNA-378; SNP; expression; target gene; gain; loss of function
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Sequence variation in a microRNA (miRNA) seed region can influence its biogenesis and effects on target mRNAs; however, in mammals, few seed region mutations leading to functional alterations have been reported to date. Here, we report the identification of a single nucleotide polymorphism (SNP) with functional consequence located in the seed region of porcine miR-378. In vitro analysis of this rs331295049 A17G SNP showed significantly up-regulated expression of the mature miR-378 (miR-378/G). In silico target prediction indicated that the SNP would modulate secondary structure and result in functional loss affecting>85% of the known target genes of the wild-type miR-378 (miR-378/A), and functional gain affecting>700 new target genes, and dual-luciferase reporter assay verified this result. This report of a SNP in the seed region of miR-378 leads to functional alteration and indicates the potential for substantive functional consequences to the molecular physiology of a mammalian organism.
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