Journal
BIORESOURCE TECHNOLOGY
Volume 250, Issue -, Pages 131-139Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.11.045
Keywords
beta-Galactosidase; Fungal strains; Lactulose; Galacto-oligosaccharides; Fructo-oligosaccharides
Funding
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2013]
- Portuguese Foundation for Science and Technology (FCT) under the COMPETE [POCI-01-0145-FEDER-006684]
- project BioTecNorte - European Regional Development Fund under the scope of Norte - Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004]
- project MultiBiorefinery - European Regional Development Fund under the scope of Norte - Programa Operacional Regional do Norte [POCI-01-0145-FEDER-016403]
- FCT [SFRH/BPD/88584/2012]
- FEDER through COMPETE - Programa Operacional Competitividade e Internacionalizacao (POCI) [POCI-01-0145-FEDER-006984]
- FCT Fundacao para a Ciencia e a Tecnologia
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beta-Galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of beta-galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective beta-galactosidase producers under submerged fermentation conditions. The crude beta-galactosidases were characterized regarding their optimal pH (3.0-5.5) and temperature (45-65 degrees C). All enzymes showed ability to synthesize lactose-based prebiotics, namely lactulose (maximal yield 3.3%) and a galacto-oligosaccharide (GOS) (maximal yield 20%). Additionally, some enzymatic extracts with fructosyltransferase activity allowed to produce other type of prebiotics, namely fructo-oligosaccharides (FOS). This work, reports for the first time the simultaneous synthesis of different mixtures of GOS (2-15% yield and 0.07-0.5 g/L.h(-1) productivity) and FOS (4-30% yield and 0.1-1 g/L.h(-1) productivity) by crude extracts exhibiting dual enzymatic activity.
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