Article
Agriculture, Dairy & Animal Science
Bence Somoskoi, Lilla Bordas, Fusa Uno, Dora Kispal, Linda Muller, Dora Torok, Sandor Cseh
Summary: The present study aimed to compare the effects of different cryopreservation methods (closed vs open vitrification) on the survival and developmental rate of canine isolated preantral follicles (PAFs). The results showed that the OPS method provided superior survival rate, in vitro growth, and hormonal production compared to the CT method. This is the first study to compare cryopreservation protocols for canine isolated preantral follicles.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Y. Wang, Z. Y. Guo, C. Zhang, D. Z. Miao, X. Y. Mao, S. M. Lu, H. M. Yang, Z. Y. Wang
Summary: This study investigated the development of ovarian follicles and the concentrations of steroid hormones in paired pigeons during the laying interval. The results showed that paired pigeons normally have 2 preovulatory follicles, with the second-largest follicle developing from the third day of the laying interval. The concentrations of P4 and T showed specific patterns during the laying interval, while E2 remained at high levels. The findings provide important insights into the regulation of ovulation and egg production in pigeons.
Article
Biochemistry & Molecular Biology
Yingjun Chen, Qinghua Liu, Ruiyan Liu, Chan Yang, Xiaodong Wang, Zaohong Ran, Shanshan Zhou, Xiang Li, Changjiu He
Summary: This study in a mouse model revealed that early folliculogenesis requires a minimum of two weeks, with different stages showing specific characteristics such as oocyte size at Type 4-5 stage and theca folliculi formation at PD10-12. The study also highlighted the importance of the PD22-23 period for studying puberty initiation, providing valuable insights into the biological events of this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yanhong Zhang, Qiuyue Chen, Yuanyuan Guo, Li Kang, Yi Sun, Yunliang Jiang
Summary: Follicle selection in chickens is a crucial process controlled by sex hormones, which impacts egg production. In this study, the phosphoproteomes of small yellow (SY) and F6 follicles were compared, leading to the identification of differentially phosphorylated proteins. The expression of LSD1Ser54p, a phosphorylated protein, was found to be upregulated in F6 follicles, indicating its involvement in follicle selection.
Article
Agriculture, Multidisciplinary
Bi Yu-lin, Yang Shu-yan, Wang Hai-yan, Chang Guo-bin, Chen Guo-hong
Summary: This study confirmed the expression of the FSH gene in chicken follicles and its role in promoting ovarian granulosa cell proliferation, enriching the theory on the HPG axis.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Obstetrics & Gynecology
Hanna Ornes Olesen, Susanne Elisabeth Pors, Lea Bejstrup Jensen, Annika Patricia Gronning, Camilla Engel Lemser, Maria Thai Hien Nguyen Heimbuerger, Linn Salto Mamsen, Natalie Getreu, Soren Tvorup Christensen, Claus Yding Andersen, Stine Gry Kristensen
Summary: Daily administration of N-acetylcysteine (NAC) for 7-12 days post-transplantation can reduce ischemia-reperfusion injury and increase follicle survival in human ovarian xenografts. This treatment works by upregulating the antioxidant defense system and exerting anti-inflammatory and antiapoptotic effects.
HUMAN REPRODUCTION
(2021)
Article
Developmental Biology
Jennifer Shannon, Aishwarya Sundaresan, Orhan Bukulmez, Zexu Jiao, Kaitlin Doody, Sarah Capelouto, Bruce Carr, Laura A. Banaszynski
Summary: Understanding how gene regulatory elements influence ovarian follicle development is crucial for addressing issues such as decreased fertility with age and short-term graft function after ovarian tissue cryopreservation. The ATAC-seq technique can identify regulatory elements important for gene regulation and fertility, but challenges remain in obtaining data from frozen samples.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Reproductive Biology
Ewa Oc, Anna Hrabia
Summary: This study investigated the differential miR expression profiles in the chicken ovary using high-throughput transcriptome analyses, revealing the potential role of miRs in regulating matrix metalloproteinase expression during follicular growth and atresia. Identified miR target pairs were associated with pathways involved in MMP inhibition, axonal guidance signaling, HIF1 alpha signaling, and GP6 signaling, providing novel insights into the molecular mechanisms underlying follicle development in chickens.
Article
Reproductive Biology
Luba Sominsky, Simin Younesi, Simone N. De Luca, Sophie M. Loone, Kylie M. Quinn, Sarah J. Spencer
Summary: The study reveals that ovarian follicles are resilient against short-term disturbances in circulating and ovarian monocytes, making them able to withstand acute changes in immune balance.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
A. D. Sales, A. B. G. Duarte, R. M. P. Rocha, I. R. Brito, Y. Locatelli, B. G. Alves, K. A. Alves, J. R. Figueiredo, A. P. R. Rodrigues
Summary: This study aimed to investigate the relative abundances of ABC transporters mRNA transcripts in ovine ovarian tissue after vitrification and in vitro culture. The results showed a negative correlation between degenerated follicles and mRNA transcript abundances of ABCB1 and ABCG2, with a significant decrease in the percentage of growing follicles after in vitro culture. Further studies are needed to explore the functions of ABC transporters in ovine folliculogenesis after in vitro culture.
Article
Biochemistry & Molecular Biology
Jingli Tao, Liangliang Zhang, Xuan Zhang, Yuanyuan Chen, Qianqian Chen, Ming Shen, Honglin Liu, Shoulong Deng
Summary: The study showed that melatonin promoted the development of secondary follicles and peripheral angiogenesis, while follicle-stimulating hormone significantly increased the number of antral follicles and peripheral angiogenesis. Melatonin and FSH may regulate the expression of VEGF and antioxidant enzymes to affect follicular development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Zeinab Ghezelayagh, Naeimeh Sadat Abtahi, Sepideh Khodaverdi, Mojtaba Rezazadeh Valojerdi, Aboulfazl Mehdizadeh, Bita Ebrahimi
Summary: The study compared agar as a cultivation substrate with matrigel-coated insert for in-situ human follicle culture. Results showed that agar and matrigel-coated inserts both supported follicular growth and survival, with agar culture group showing higher percentage of growing follicles, lower apoptotic index, and significantly higher expression of proliferative marker Ki67.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xue Sun, Xiaoxia Chen, Jinghua Zhao, Chang Ma, Chunchi Yan, Simushi Liswaniso, Rifu Xu, Ning Qin
Summary: This study conducted a comparative mRNA transcriptomic analysis of ovarian follicles at key developmental stages in chickens with different egg production levels, identifying several key genes and signaling pathways involved in the regulation of ovarian follicle development and egg production traits. The study provided valuable insights into the molecular mechanisms underlying egg production in chickens.
Article
Medicine, General & Internal
Ronald Peek, Lotte L. Eijkenboom, Didi D. M. Braat, Catharina C. M. Beerendonk
Summary: Short-term exposure to Everolimus has been shown to effectively eliminate Ewing's sarcoma tumor foci in ovarian tissue without causing harm to follicle morphology, survival, or early folliculogenesis. This suggests that pharmacological ex vivo purging of ovarian cortex tissue from contaminating ES tumor foci before autotransplantation may reduce the risk of reintroducing malignancy.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Obstetrics & Gynecology
Bingying Xu, Zhuqing Li, Shan Li, Hanni Ke, Qian Zhang, Yingying Qin, Ting Guo
Summary: This study identified pathogenic variants of the TSC2 gene in patients with POI and suggested the involvement of defective TSC/mTOR pathway-mediated primordial follicle hyperactivation in the pathogenesis of POI.
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
L. Cacciottola, A. Camboni, A. Cernogoraz, J. Donnez, M. M. Dolmans
Summary: The size of the ovarian follicle pool is regulated by both apoptosis and autophagy. Apoptosis is responsible for the physiological decline of the follicle pool over time and increases in the event of prior chemotherapy. Autophagy plays a major role in the diminution of the follicle pool before puberty.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Christina Anna Stratopoulou, Sophie Cussac, Marie d'Argent, Jacques Donnez, Marie-Madeleine Dolmans
Summary: This study found that M2 macrophages play an important role in the invasiveness of adenomyosis. M2 macrophages accumulate more in patients with adenomyosis compared to healthy tissue. Co-culture with M2 macrophages significantly enhances the invasion capacity of endometrial cells. The study did not find any gene expression differences indicating epithelial-mesenchymal transition (EMT).
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Jacques Donnez, Marie-Madeleine Dolmans
Summary: IVF cannot replace reproductive surgery because reproductive surgery can heal or harm the organs involved in reproduction. This paper explores different diseases and conditions that affect fertility, and highlights the potential benefits of technological innovations, novel applications, and the expertise of reproductive surgeons. The promise of restoring functional anatomy for enhanced pregnancy chances keeps reproductive surgery alive, and it is our responsibility to properly train young residents to provide the right treatment at the right time.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Ronald Peek, Sapthami Nadesapillai, Thu Yen Thi Nguyen, Sarah Vassart, Dominique Smeets, Guillaume van de Zande, Alessandra Camboni, Didi Braat, Janielle van der Velden, Jacques Donnez, Kathrin Fleischer, Marie-Madeleine Dolmans
Summary: The impact of aneuploid granulosa and stromal cells on folliculogenesis of small ovarian follicles from patients with mosaic Turner syndrome (TS) was studied using a murine xenograft model. It was found that despite the presence of aneuploid cells, small follicles from TS patients were able to undergo normal development, suggesting that ovarian tissue cryopreservation could be a valid option to preserve fertility in young TS patients, especially before the age of 12.
FERTILITY AND STERILITY
(2023)
Review
Obstetrics & Gynecology
Dhoha Kourta, Marc Kanbar, Christiani A. Amorim, Christine Wyns
Summary: Restoration of fertility is crucial for childhood hematological cancer survivors. However, there is a risk of contamination of the gonads by cancer cells, especially in leukemia and lymphoma patients. More sensitive techniques are needed to ensure the safety of transplanting cryostored testicular and ovarian tissues or cells back to the patient. Methods to eliminate neoplastic cells are urgently needed. This review presents contamination rates and decontamination methods for gonadal tissues in leukemia or lymphoma cases.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
F. Vitale, L. Cacciottola, F. S. Yu, M. Barretta, C. Hossay, J. Donnez, M. M. Dolmans
Summary: There is a difference in viability and quality of human follicles cultured in ovarian cortex between 20% and 5% oxygen tension. The viability and quality of follicles are higher under 5% oxygen tension after 6 days of in vitro culture (IVC).
HUMAN REPRODUCTION
(2023)
Article
Genetics & Heredity
Lara Houeis, Marie-Madeleine Dolmans
Summary: The 7th International Congress of the ISFP took place in Brussels in November 2022, attracting hundreds of attendees from around the world. Distinguished leaders in the field discussed the latest advances and participants had the opportunity to attend workshops guided by skilled practitioners. Topics covered included fertility preservation approaches in cancer and benign pathologies, male factor infertility, in vitro maturation and poor responders, the impact of chemotherapy on the ovary, and future perspectives.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Genetics & Heredity
Elham Hosseini, Hamid-Reza Kohan-Ghadr, Mahshid Bazrafkan, Christiani A. A. Amorim, Maryam Askari, Armin Zakeri, Seyedeh Neda Mousavi, Raheleh Kafaeinezhad, Parivash Afradiasbagharani, Sahar Esfandyari, Mahboobeh Nazari
Summary: As the COVID-19 pandemic continues, there is growing concern about its effects on reproductive organs, infertility, and future generations. This review article focuses on the virology of SARS-CoV-2, its impact on the activation of the NLRP3 inflammasome, and its connection to reproductive disorders. It also discusses potential therapeutic approaches for mitigating the long-term repercussions of COVID-19, particularly by inhibiting the NLRP3 inflammasome.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Chemistry, Medicinal
Jacques Donnez, Luciana Cacciottola, Jean-Luc Squifflet, Marie-Madeleine Dolmans
Summary: Estrogens play a critical role in the pathogenesis of endometriosis, and lowering estradiol levels with oral GnRH antagonists, such as linzagolix, can be an effective option for managing symptomatic endometriosis in premenopausal women. Linzagolix is an orally administered GnRH receptor antagonist that reduces LH and FSH production, leading to a dose-dependent decline in estrogen. Phase 2 and 3 trials have shown promising results for the use of linzagolix in the treatment of endometriosis.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2023)
Review
Engineering, Biomedical
Ellen C. R. Leonel, Arezoo Dadashzadeh, Saeid Moghassemi, Hanne Vlieghe, Christine Wyns, Renan Orellana, Christiani A. Amorim
Summary: Acquired disorders and congenital defects of the male and female reproductive systems can have profound impacts on patients, causing sexual and endocrine dysfunction and infertility, as well as psychosocial consequences that affect their self-esteem, identity, sexuality, and relationships. Reproductive tissue engineering (REPROTEN) is a promising approach to restore fertility and improve the quality of life of patients with reproductive disorders by developing, replacing, or regenerating cells, tissues, and organs from the reproductive and urinary systems. This review explores the latest advancements in REPROTEN techniques and their applications for addressing degenerative conditions in male and female reproductive organs.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Genetics & Heredity
Marta Barretta, Luciana Cacciottola, Camille Hossay, Jacques Donnez, Marie-Madeleine Dolmans
Summary: The impact of processing human ovarian tissue on follicle activation dynamics was investigated in this study. It was found that ovarian tissue manipulation significantly affected follicle dynamics by stimulating the PI3K and Hippo signaling pathways involved in primordial follicle activation.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Oncology
Matthieu de Codt, Pascale Jadoul, Mathieu Luyckx, Jean-Luc Squifflet, Marie-Madeleine Dolmans, Charlotte Maillard, Jean-Francois Baurain, Etienne Marbaix, Amandine Gerday
Summary: This study presents the results of hysteroscopic resection for the management of hydatidiform mole in 36 patients. The main complications encountered during the surgery were uterine perforation and glycine resorption. Retained product of conception was found in six patients during the ultrasound control one month after the intervention. In conclusion, hysteroscopic resection is a feasible procedure for the treatment of molar pregnancy.
Article
Obstetrics & Gynecology
Saeid Moghassemi, Arezoo Dadashzadeh, Alessandra Camboni, Olivier Feron, Ricardo Bentes Azevedo, Christiani A. Amorim
Summary: Our study demonstrates the feasibility of using photodynamic therapy (PDT) to eliminate leukemia cells from ovarian tissue (OT) fragments before transplantation.
HUMAN REPRODUCTION OPEN
(2023)
Article
Obstetrics & Gynecology
Arezoo Dadashzadeh, Saeid Moghassemi, Alexis Peaucelle, Carolina M. Lucci, Christiani A. Amorim
Summary: This study developed a tailored hydrogel made of synthetic and natural polymers that mimicked the mechanical properties of the human ovarian cortex. The results showed that this PEGylated fibrin hydrogel significantly improved the growth of preantral follicles in vitro.
HUMAN REPRODUCTION OPEN
(2023)
Review
Obstetrics & Gynecology
L. Cacciottola, F. Vitale, J. Donnez, M. M. Dolmans
Summary: Regenerative medicine with stem cell therapy has shown promising results in protecting ovarian reserve and improving fertility potential.
HUMAN REPRODUCTION OPEN
(2023)