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Agricultural Engineering
Joanna Chwialkowska, Lukasz Smaga, Piotr Oleskowicz-Popiel
Summary: Open culture fermentation (OCF) is a promising technology for converting different organic waste into carboxylic acids (CAs) and alcohols in biorefinery. This study found that for complex substrates like raw maize silage, OCF can achieve the highest CA yield, while for monosaccharides, co-fermentation (glucose/galactose) is the most efficient process.
BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Olgun Guvench, Devon Martin, Megan Greene
Summary: The conformational properties of carbohydrates play an important role in protein structure, but studying carbohydrate-containing systems presents challenges. Molecular dynamics simulations provide a detailed view of biomolecular dynamics, but the accuracy depends on the quality of the force field used. This study shows that the CHARMM force field can accurately model the ring puckering of carbohydrates, which is important for accurately modeling carbohydrate-containing vertebrate biomolecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Victoria D. S. Freitas, Ana Paez, Pascal Fongarland, Regis Philippe, Lea Vilcocq
Summary: This paper investigates the hydrogenation of different sugars derived from hemicelluloses using a Ru/TiO2 catalyst. The study explores the effects of temperature, pressure, sugar concentration, and catalyst amount on xylose hydrogenation, and proposes a Langmuir-Hinshelwood kinetic model. Comparative analysis of five hemicellulose sugars reveals that the sugar structure significantly impacts the hydrogenation rate, with hexoses reacting slower than pentoses.
Article
Biotechnology & Applied Microbiology
Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen, Leila Lo Leggio
Summary: This study characterized the most thermophilic endo-beta-1,4-galactanase known to date and successfully engineered it to modify the degradation profile while maintaining its desirable thermostability. The length of oligosaccharide products is an important property for both industrial and natural GHs.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemical Research Methods
Laetitia Collet, Corinne Vander Wauven, Yamina Oudjama, Moreno Galleni, Raphael Dutoit
Summary: Transglycosylating glycoside hydrolases (GHs) have great potential for the synthesis of oligosaccharides. This study examines the enzymatic and structural characteristics of RBcel1, a GH5_5 subfamily transglycosylase. The size of the acceptor and donor sugars, as well as the reaction pH, were found to be crucial factors in transglycosylation efficiency. The structural analysis of RBcel1 variants provided insights into the molecular factors governing transglycosylation.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Vy Ha Nguyen Tran, Thuan Thi Nguyen, Sebastian Meier, Jesper Holck, Hang Thi Thuy Cao, Tran Thi Thanh Van, Anne S. Meyer, Maria Dalgaard Mikkelsen
Summary: This study cloned an endo-alpha(1,3)-fucoidanase encoding gene Mef2 from a marine bacterium and characterized its functional properties, showing its activity and specificity in fucoidan hydrolysis, as well as unveiling new structural details.
Article
Biochemistry & Molecular Biology
Dayanand C. Kalyani, Tom Reichenbach, Markus M. Keskitalo, Julian Conrad, Henrik Aspeborg, Christina Divne
Summary: The gut microbiome of the termite Reticulitermes flavipes contains a symbiont Opitutaceae bacterium strain TAV5, which produces an enzyme called Op5Man5 that is highly specific for beta-1,4-mannosidic bonds in mannooligosaccharides and ivory nut mannan. Op5Man5 is the first example of a GH5 glycoside hydrolase with a GH42 beta-galactosidase-type homotrimeric structure, despite insignificant sequence similarity with other enzymes.
JOURNAL OF STRUCTURAL BIOLOGY-X
(2021)
Article
Plant Sciences
Lucia Albornos, Ignacio Martin, Josefina Hernandez-Nistal, Emilia Labrador, Berta Dopico
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Plant Sciences
Lucia Izquierdo, Ignacio Martin, Lucia Albornos, Josefina Hernandez-Nistal, Pablo Hueso, Berta Dopico, Emilia Labrador
JOURNAL OF PLANT PHYSIOLOGY
(2018)
Article
Plant Sciences
Maria Moneo-Sanchez, Alejandro Alonso-Chico, J. Paul Knox, Berta Dopico, Emilia Labrador, Ignacio Martin
Article
Multidisciplinary Sciences
Sonia Mediavilla, Ignacio Martin, Josefa Babiano, Alfonso Escudero
Article
Plant Sciences
L. Albornos, I. Martin, E. Labrador, B. Dopico
BIOLOGIA PLANTARUM
(2019)
Article
Forestry
S. Mediavilla, I Martin, A. Escudero
EUROPEAN JOURNAL OF FOREST RESEARCH
(2020)
Article
Plant Sciences
Maria Moneo-Sanchez, Andrea Vaquero-Rodriguez, Josefina Hernandez-Nistal, Lucia Albornos, Paul Knox, Berta Dopico, Emilia Labrador, Ignacio Martin
Article
Plant Sciences
Lucia Albornos, Virginia Casado-del-Castillo, Ignacio Martin, Jose M. Diaz-Minguez, Emilia Labrador, Berta Dopico
Summary: The ST1 and ST6 proteins may play a role in primary and lateral root development, as well as symbiotic nodule development. However, only ST6 is involved in the interaction with hemibiotrophic fungi. Their expression is modulated in different ways during plant development and in response to biotic agents, indicating distinct roles in these processes.
Article
Plant Sciences
Sonia Mediavilla, Ignacio Martin, Alfonso Escudero
Summary: The study compared vein and stomatal traits of seedlings and adults of three Mediterranean Quercus species. The results showed that minor vein density and vein volume per area increased with tree age, which was inconsistent with the ontogenetic trends in gas-exchange rates, supporting the hypothesis of lower investments in vascular tissues at the seedling stage.
JOURNAL OF PLANT ECOLOGY
(2021)