Article
Cell Biology
Kwantae Kim, Jameson Orvis, Alberto Stolfi
Summary: Pax3/7 factors play crucial roles in the development of the nervous system in vertebrates and are also involved in similar functions in the model tunicate Ciona. The study reveals that Pax3/7 is expressed in the neural plate borders and central nervous system after neural tube closure, playing a conserved role in neural tube closure and neuron specification. These results suggest that the key functions of Pax3/7 in neural tube development are evolutionarily ancient, dating back to the common ancestor of vertebrates and tunicates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shufang Cai, Bin Hu, Qi Zhu, Tianqi Duo, Xiaoyu Wang, Xian Tong, Xiaorong Luo, Renqiang Yuan, Yaosheng Chen, Jing Wang, Chenglong Luo, Baosong Xing, Delin Mo
Summary: Delving into porcine embryonic myogenesis is crucial for understanding the complex regulation of breed-specific differences in growth performance and meat production. This study reveals that pigs with less meat production exhibit earlier embryonic myogenesis, and the Notch signaling pathway plays a crucial role in regulating myogenic progenitor maintenance and differentiation. Inhibition of Notch signaling promotes myogenic differentiation and accelerates the process of myogenic progenitors differentiating into myoblasts. These findings provide valuable insights into the underlying mechanisms of breed-specific differences in meat production.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Biochemistry & Molecular Biology
Omid Mashinchian, Filippo De Franceschi, Sina Nassiri, Joris Michaud, Eugenia Migliavacca, Patrick Aouad, Sylviane Metairon, Solenn Pruvost, Sonia Karaz, Paul Fabre, Thomas Molina, Pascal Stuelsatz, Nagabhooshan Hegde, Emmeran Le Moal, Gabriele Dammone, Nicolas A. Dumont, Matthias P. Lutolf, Jerome N. Feige, C. Florian Bentzinger
Summary: Microenvironmental interactions play a crucial role in directing the fate decisions of human-induced pluripotent stem cells (hiPSCs) towards myogenic lineage. In this study, researchers engineered a complex three-dimensional niche using different cell types found in the embryonic or adult stem cell niche to promote myogenic specification of hiPSCs. The study identified embryonic endothelial cells and fibroblasts as highly permissive for myogenic differentiation of hiPSCs. This two-week protocol resulted in the derivation of Pax7-positive myogenic progenitors, which showed enhanced muscle function and regenerative potential when transplanted into a mouse model of Duchenne muscular dystrophy.
Article
Biochemistry & Molecular Biology
Mengya Wang, Weihao Song, Chaofan Jin, Kejia Huang, Qianwen Yu, Jie Qi, Quanqi Zhang, Yan He
Summary: Pax3 and Pax7 are crucial for muscle development, with distinct populations of muscle satellite cells in black rockfish marked by SsPax3 and SsPax7. SsPax3a and two SsPax7 genes show increased expression during muscle repair, while SsPax3b remains unchanged, suggesting a more complex muscle stem cell population in teleosts compared to mammals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Christine M. Heske, Yueh-Yun Chi, Rajkumar Venkatramani, Minjie Li, Michael A. Arnold, Roshni Dasgupta, Susan M. Hiniker, Douglas S. Hawkins, Leo Mascarenhas
Summary: In patients with localized, FOXO1 fusion-positive RMS, clinical factors such as age at diagnosis and tumor size can impact survival outcomes, with older age and larger tumors being adverse prognostic factors for EFS. Patients with both older age and large tumor size experience significantly inferior outcomes.
Article
Genetics & Heredity
Line J. Manceau, Julien E. Richard Albert, Pier-Luigi J. Lollini, Maxim V. C. E. Greenberg, Pascale J. Gilardi-Hebenstreit, Vanessa E. Ribes
Summary: The chimeric proteins PAX3-FOXO1 and PAX7-FOXO1, derived from chromosomal translocations, exhibit distinct genomic occupancy and transcriptional activity, leading to different pathological manifestations in alveolar rhabdomyosarcoma.
Article
Multidisciplinary Sciences
Takuyo Kanayama, Mitsuru Miyachi, Yohei Sugimoto, Shigeki Yagyu, Ken Kikuchi, Kunihiko Tsuchiya, Tomoko Iehara, Hajime Hosoi
Summary: The study found that PAX3-FOXO1 is associated with B7-H3 in alveolar rhabdomyosarcoma, with PAX3-FOXO1 positively regulating B7-H3 expression. Both are involved in multiple pathways related to aggressiveness in ARMS, such as cell migration and myogenic differentiation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Bingqing Huo, Yang Yang, Manhui Li, Jun Wan, Wei Zhang, Bo Yu, Xiaofan Chen
Summary: Pax3 inhibits cell viability and proliferation, affects cell cycle and neurite outgrowth in Neuro-2a cells; Pax7 overexpression has no significant impact on cell viability and proliferation; Pax3 up-regulated genes are involved in cell cycle machinery, potentially revealing the mechanism of Neuro-2a cell proliferation.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Biotechnology & Applied Microbiology
Soeren S. Huettner, Henriette Henze, Dana Elster, Philipp Koch, Ursula Anderer, Bjoern von Eyss, Julia von Maltzahn
Summary: The expression levels of the transcriptional repressor TRPS1 are specifically enhanced in the embryonal subtype of rhabdomyosarcoma, resulting in impaired myogenic differentiation and tumor growth. Reduction of TRPS1 levels may be a therapeutic approach to drive embryonal rhabdomyosarcoma cells into myogenic differentiation.
Review
Cell & Tissue Engineering
Dengjie Yu, Zijun Cai, Daishi Li, Yi Zhang, Miao He, Yuntao Yang, Di Liu, Wenqing Xie, Yusheng Li, Wenfeng Xiao
Summary: Stem cells are crucial for regenerative medicine, particularly in skeletal muscle regeneration, as they have the potential to repair damaged muscle tissues and alleviate patient suffering. This review discusses the use of scaffolds and stem cells in skeletal muscle regeneration and proposes ideas for future development.
STEM CELLS INTERNATIONAL
(2021)
Review
Plant Sciences
Christopher R. Buckley, Xiang Li, Maria Carmen Marti, Michael J. Haydon
Summary: Plants adapt their metabolism to environmental fluctuations through circadian clocks, which play a crucial role in anticipating and responding to predictable and unpredictable challenges. Metabolism is now seen as an integrated feature of the plant circadian system, serving as both an output and an input to this dynamic timekeeping mechanism. The spatial and temporal aspects of metabolism raise interesting questions about its influence on circadian timekeeping. Various mechanisms, including metabolic signaling pathways and direct modifications of clock genes or proteins, have been found to influence the transcription-translation feedback loops of the circadian oscillator. These metabolic feedback loops contribute to the coordination of rhythmic metabolism and play a role in daily, developmental, and seasonal timekeeping.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Pediatrics
Mark C. Xu, M. Owais Ghani, Annie Apple, Heidi Chen, Martin Whiteside, Scott C. Borinstein, Hernan Correa, Harold N. Lovvorn
Summary: This study found that FXR1 expression was universally strong in RMS specimens at diagnosis regardless of disease or patient characteristics, particularly in undifferentiated cells. Reduction in FXR1 expression after chemotherapy was associated with improved survival for embryonal and translocation-negative RMS patients.
JOURNAL OF PEDIATRIC SURGERY
(2021)
Article
Oncology
Chuanhong Zhong, Lilei Peng, Bei Tao, Senlin Yin, Liang Lyu, Hao Ding, Xiaobo Yang, Tangming Peng, Haiping He, Peizhi Zhou
Summary: This study investigated the specific role of TDO2 in glioma progression and found that high TDO2 expression was closely associated with poor prognosis in glioma patients. TDO2 promoted glioma cell proliferation by increasing the levels of tryptophan metabolite Kyn through the AhR/AKT signaling pathway and had immunosuppressive effects.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kun Zhang, Chunfang Yu, Ruoxi Tian, Wancong Zhang, Shijie Tang, Guiying Wang
Summary: This study found that PAX3 is overexpressed in SKCM and is associated with poor prognosis. Knockdown of PAX3 inhibits proliferation, invasion, and migration of SKCM cells and reverses tumor cell EMT. PAX3 may serve as a potential therapeutic target and prognostic biomarker for SKCM.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
M. T. Ciubuc-Batcu, N. J. C. Stapelberg, J. P. Headrick, G. M. C. Renshaw
Summary: The nervous system relies on mitochondria, and impaired mitochondrial function is associated with major depressive disorder. Modulating mitochondrial function may be a therapeutic target for treating MDD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Correction
Biochemistry & Molecular Biology
Saowaluk Saisomboon, Ryusho Kariya, Piyanard Boonnate, Kanlayanee Sawanyawisuth, Ubon Cha'on, Vor Luvira, Yaovalux Chamgramol, Chawalit Pairojkul, Wunchana Seubwai, Atit Silsirivanit, Sopit Wongkham, Seiji Okada, Sarawut Jitrapakdee, Kulthida Vaeteewoottacharn
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Pavan Thapak, Zhe Ying, Victoria Palafox-Sanchez, Guanglin Zhang, Xia Yang, Fernando Gomez-Pinilla
Summary: Traumatic brain injury (TBI) impairs cellular energy demand, compromising neuronal function and plasticity. This study demonstrates that the mitochondrial activator humanin (HN) can counteract the reduction in mitochondrial bioenergetics caused by TBI, restore memory function and synaptic protein levels, and suppress inflammation and astrocyte proliferation. HN plays an integral role in normalizing fundamental aspects of TBI pathology.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Paul Murphy, Valeria A. Buzinova, Carrie E. Johnson
Summary: Progress has been made in the treatment of Alzheimer's disease through the development of anti-A beta therapeutics, which have shown modest efficacy in slowing the progression of the disease. However, the puzzling issue remains as to why completely removing A beta does not fully stop the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yang Zhang, Mengqiu Hao, Xuyang Yang, Su Zhang, Junhong Han, Ziqiang Wang, Hai-Ning Chen
Summary: Colorectal cancer often requires adjuvant therapies to reduce tumor burden, and the efficacy of these therapies is significantly influenced by reactive oxygen species (ROS). ROS-mediated colorectal cancer adjuvant therapies involve multiple mechanisms, and preliminary clinical trials have shown the potential of ROS-manipulating therapy in enhancing treatment outcomes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Mengxin Li, Xuanzhong Wang, Xuyang Chen, Jinghui Hong, Ye Du, Dong Song
Summary: Pancreatic adenocarcinoma (PAAD) is a common digestive malignant tumor with limited treatment options. This study demonstrates that TGM2 may serve as a marker for treatment and prognosis in pancreatic cancer patients. Co-treatment of low dose cisplatin (DDP) and the TGM2 inhibitor GK921 effectively inhibits PAAD cell viability and proliferation in vitro and in vivo, by inhibiting epithelial-to-mesenchymal transition (EMT) induced by TGM2 and enhancing cell cycle arrest and apoptosis caused by DDP. These findings suggest that the combination of GK921 and DDP holds promise as a treatment for PAAD patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Liaoran Niu, Qi Wang, Fan Feng, Wanli Yang, Zhenyu Xie, Gaozan Zheng, Wei Zhou, Lili Duan, Kunli Du, Yiding Li, Ye Tian, Junfeng Chen, Qibin Xie, Aqiang Fan, Hanjun Dan, Jinqiang Liu, Daiming Fan, Liu Hong, Jian Zhang, Jianyong Zheng
Summary: This review provides a comprehensive summary of the interaction between cancer cells and macrophages in the tumor microenvironment, and discusses the role of small extracellular vesicles (sEVs) in this process. It also explores the various effects of macrophage-secreted sEVs on tumor malignant transformation, and addresses the therapeutic advancements and challenges associated with these vesicles.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Neha Sawant, Sudhir Kshirsagar, P. Hemachandra Reddy, Arubala P. Reddy
Summary: Depression is a common neuropsychiatric comorbidity in Alzheimer's disease (AD) and other Tauopathies. Selective serotonin reuptake inhibitor (SSRI) treatment, such as Citalopram, not only has anti-depressive and anxiolytic effects, but also helps improve neurogenesis, reduce amyloid burden & Tau pathologies, and neuroinflammation in AD. In this study, Citalopram was found to reduce pathologically pTau level, increase synaptic gene expression and cytoskeletal structure, as well as improve cell survival, mitochondrial respiration, and mitochondrial morphology in cells expressing mutant APP and Tau. These findings suggest that Citalopram could be a promising therapeutic drug for treating depression and AD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Chen, Jiulin Tan, Chuan Yang, Zhiguo Ling, Jianzhong Xu, Dong Sun, Fei Luo
Summary: Bone is a self-healing organ that undergoes continuous regeneration through the cooperation of osteoclasts and osteoblasts. This study used ATAC-seq and RNA-Seq techniques to investigate the chromatin accessibility and transcriptomic landscape of osteoblast differentiation and mineralization. The results showed that global chromatin accessibility was extensively improved during osteoblastogenesis. Additionally, several transcription factors including MEF2A, PRRX1, Shox2, and HOXB13 were found to modulate the promoter accessibility of target genes during osteoblast differentiation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Zi-Ran Kang, Shanshan Jiang, Ji-Xuan Han, Yaqi Gao, Yile Xie, Jinxian Chen, Qiang Liu, Jun Yu, Xin Zhao, Jie Hong, Haoyan Chen, Ying-Xuan Chen, Huimin Chen, Jing-Yuan Fang
Summary: The study demonstrates that BCAA metabolism is involved in the development of colorectal cancer (CRC). BCAT2 deficiency promotes CRC progression by inhibiting BCAA metabolism and chronically activating the mTORC1 pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Chao Zheng, Lingling Liu, Caiyun Liu, Fengna Chu, Yue Lang, Shan Liu, Yan Mi, Jie Zhu, Tao Jin
Summary: Inducing tolerogenic dendritic cells (tDCs) with low RelB expression could effectively alleviate symptoms and reduce immune cell infiltration and demyelination in experimental autoimmune encephalomyelitis (EAE) mouse model.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Hang Lam Li, Simei Go, Jung-Chin Chang, Arthur Verhoeven, Ronald Oude Elferink
Summary: This review highlights the distinct characteristics and crucial role of soluble adenylyl cyclase (sAC) in cellular processes, as well as recent significant advancements in the field of sAC research.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
M. Seco-Cervera, D. Ortiz-Masia, D. C. Macias-Ceja, S. Coll, L. Gisbert-Ferrandiz, J. Cosin-Roger, C. Bauset, M. Ortega, B. Heras-Moran, F. Navarro-Vicente, M. Millan, J. V. Esplugues, S. Calatayud, M. D. Barrachina
Summary: The study revealed the presence of resistance to apoptosis in complicated ileal Crohn's disease, with PDGFB inducing an ETS1-mediated resistance to apoptosis associated with an inflammatory and fibrogenic pattern of expression in intestinal fibroblasts. Potential targets against ileal fibrosis include PDGFRB, IL1R1, or MCL1.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Review
Biochemistry & Molecular Biology
Yunmeng Wang, Ping Cheng
Summary: Oncolytic viruses (OVs) are emerging as therapeutically relevant anticancer agents, especially when combined with genetically modified bispecific T cell engagers (BiTEs). This combination strategy can overcome the limitations of BiTEs alone and provide targeted cytotoxicity to solid tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Stephanie Tannous, Hassan Y. Naim
Summary: Congenital sucrase-isomaltase deficiency (CSID) is an autosomal recessive disorder caused by variants in the SI gene. A frameshift mutation called c.273_274delAG (p.Gly92Leufs*8) has been identified in CSID patients in Greenlandic population, which leads to loss of digestive function of SI. Surprisingly, the truncated mutant can still be located on the cell surface and interacts with wild type SI, negatively affecting its enzymatic function. Furthermore, heterozygote carriers of this mutation may also exhibit CSID symptoms.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)