Article
Cell Biology
Thomas Mueller, Eugen Schlegel, Stephanie Zingler, Hans Michael Thiede
Summary: The catechol-O-methyltransferase inhibitors entacapone and opicapone prolong the efficacy of conventional oral levodopa/dopa decarboxylase inhibitor formulations through an increase in levodopa plasma bioavailability. The study compared the impact of single-day entacapone or opicapone intake on the pharmacokinetic plasma behavior of levodopa, 3-O-methyldopa, and total homocysteine in Parkinson's disease patients. It was found that opicapone had higher levodopa bioavailability and better baseline scores compared to entacapone.
Article
Biochemical Research Methods
Mehdi Tahmasebi, Taravat Bamdad, Winnie Edith Svendsen, Mehdi Forouzandeh-Moghadam
Summary: In this study, a passive paper-based vertical flow assay was developed for the detection of nucleic acid targets, achieving high specificity and sensitivity. The results showed 100% consistency with real-time PCR, demonstrating the potential of this method for nucleic acid detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Clinical Neurology
Alfonso Fasano, Rocio Garcia-Ramos, Tanya Gurevich, Robert Jech, Lars Bergmann, Olga Sanchez-Solino, Juan Carlos Parra, Mihaela Simu
Summary: A multinational retrospective study found that long-term use of levodopa-carbidopa intestinal gel (LCIG) can improve motor and nonmotor symptoms in patients with Parkinson's disease, while potentially reducing the need for additional medications.
JOURNAL OF NEUROLOGY
(2023)
Article
Clinical Neurology
Francesca Morgante, Valentina Oppo, Margherita Fabbri, Enrica Olivola, Chiara Sorbera, Rosa De Micco, Giovanna Chiara Ielo, Fabiana Colucci, Salvatore Bonvegna, Alessio Novelli, Nicola Modugno, Mariachiara Sensi, Maurizio Zibetti, Leonardo Lopiano, Alessandro Tessitore, Manuela Pilleri, Roberto Cilia, Antonio E. Elia, Roberto Eleopra, Lucia Ricciardi, Giovanni Cossu
Summary: The study showed that LCIG infusion can be effective for late elderly Parkinson's disease patients, with no significant differences in quality of life, motor and non-motor symptoms severity, side effects, and dropout rates compared to younger patients.
JOURNAL OF NEUROLOGY
(2021)
Article
Chemistry, Analytical
Chonghui Wei, Xuan Xie, Yue Mou, Shiqi Cheng, Jin Yang, Kaixin Xue, Kewei Yu, Xinru Lin, Chunfen Zhang, Yujie Zhao, Xingyu Luo, Yilin Wang
Summary: Researchers have successfully synthesized MoS2@TiO2 nanocomposites (MoS2@TiO2 NCs) using a simple synthesis method, in which molybdenum disulfide (MoS2) and titanium dioxide (TiO2) are controllably integrated. MoS2@TiO2 NCs exhibit significantly enhanced peroxidase-like activity compared to single MoS2 or natural enzyme. By catalyzing the decomposition of H2O2 and competing with a chromogenic agent for hydroxyl radicals (OH), a sensitive, specific, and colorimetric analysis of dopamine (DA) is achieved. The visual detection capability of the method enables convenient, sensitive, and naked-eye detectable research on cellular DA secretion.
Article
Chemistry, Analytical
Jisoo Lee, Seonki Hong
Summary: In this study, we present a novel strategy for detecting biomarkers rapidly and conveniently using the naked eye. We utilize the characteristics of quinone tanning, a biomimetic process, to control the gelation of biomolecules, which serves as a visible indication of the presence of biomarkers. As a demonstration, we show that this method can be applied to detect high concentrations of glucose in human serum using a simple capillary test visualized with the naked eye.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Behavioral Sciences
K. Amande M. Pauls, Jussi Toppila, Maija Koivu, Johanna Eerola-Rautio, Marianne Udd, Eero Pekkonen
Summary: The study found that 10.5% of PD patients in Finland developed new-onset polyneuropathy after starting treatment with LCIG. Additionally, patients who experienced significant weight loss greater than 1.5 were more likely to develop neuropathy, while oral B-vitamin substitution may help prevent polyneuropathy related to LCIG treatment.
BRAIN AND BEHAVIOR
(2021)
Review
Clinical Neurology
Taiji Tsunemi, Genko Oyama, Shinji Saiki, Taku Hatano, Jiro Fukae, Yasushi Shimo, Nobutaka Hattori
Summary: LCIG treatment shows significant efficacy in reducing OFF time, increasing ON time, and improving some nonmotor symptoms and quality of life in Parkinson's disease patients. Discontinuation rates are mainly related to device issues, but the convenience of discontinuation with LCIG may be an advantage.
MOVEMENT DISORDERS
(2021)
Article
Medical Laboratory Technology
Charles R. Lefevre, Felipe Le Divenah, Nicolas Collet, Romain Pelletier, Eric Robert, Martine Ropert, Maxime Pawlowski, Thomas Gicquel, Claude Bendavid
Summary: This study found that the commonly used creatininase-peroxidase method for measuring creatinine is affected by N-acetylcysteine (NAC) interference at therapeutic NAC levels, while the creatininase-amperometric method is not affected. Therefore, the enzymo-amperometric method is suitable for measuring creatinine in APAP intoxicated patients undergoing NAC therapy, preventing false hypocreatininemia.
CLINICA CHIMICA ACTA
(2023)
Article
Health Care Sciences & Services
Helga Maria Gretarsdottir, Erik Widman, Anders Johansson, Dag Nyholm
Summary: The study evaluated the individual experience of LC-5 in clinical practice, showing that the majority of patients reported symptom improvement with favorable usability. The results suggest that LC-5 therapy is generally well-tolerated and may offer a personalized approach for fine-tuned PD treatment.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Nanoscience & Nanotechnology
Wei Wang, Jinyang Zheng, Hao Zhou, Qiang Liu, Li Jia, Xiuming Zhang, Dongtao Ge, Wei Shi, Yanan Sun
Summary: Overproduction of ROS and cumulative oxidative stress contribute to the degeneration of dopaminergic neurons in PD patients. Polydopamine (PDA), with its redox property, has been studied for its antioxidant enzyme mimetic activities. By co-synthesizing PDA with selenocystine, a nanocomposite (PDASeCys) with GPx-like activity was prepared. The PDASeCys nanocomposite showed better free radical scavenging efficiency and additional GPx enzymatic activity compared to PDA. In vitro and in vivo experiments demonstrated the protective effect of PDASeCys on neuronal cells and its ability to inhibit Parkinson-related symptoms in mice.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Clinical Neurology
Konstantin Senkevich, Cornelia E. Zorca, Aliza Dworkind, Uladzislau Rudakou, Emma Somerville, Eric Yu, Alexey Ermolaev, Daria Nikanorova, Jamil Ahmad, Jennifer A. Ruskey, Farnaz Asayesh, Da Spiegelman, Stanley Fahn, Cheryl Waters, Oury Monchi, Yves Dauvilliers, Nicolas Dupre, Lior Greenbaum, Sharon Hassin-Baer, Francis P. Grenn, Ming Sum Ruby Chiang, S. Pablo Sardi, Benoit Vanderperre, Cornelis Blauwendraat, Jean-Francois Trempe, Edward A. Fon, Thomas M. Durcan, Roy N. Alcalay, Ziv Gan-Or
Summary: Senkevich et al. provide evidence that GALC is the gene associated with Parkinson's disease at the chromosome 14 locus and suggest that the association of variants at the GALC locus may be driven by their effect of increasing galactosylceramidase expression and activity. The study highlights the role of lysosome in Parkinson's disease pathogenesis.
Article
Clinical Neurology
Rajesh Pahwa, Aristide Merola, Michael Soileau, Ali Alobaidi, A. Simon Pickard, Prasanna L. Kandukuri, Yanjun Bao, John Strezewski, Julia Oddsdottir, Weiwei Xu, David Standaert
Summary: This study aims to compare the cost, quality-adjusted life years (QALY), and cost-effectiveness of Carbidopa/levodopa enteral suspension (CLES) and the standard-of-care (SoC) for Parkinson's disease (PD) patients in the US. The results showed that although CLES is associated with incremental costs, it provides greater QALY gain, making it a cost-effective treatment option for PD patients with medication-resistant motor fluctuations.
MOVEMENT DISORDERS
(2023)
Review
Clinical Neurology
Heinz Reichmann
Summary: The aim of long-term levodopa therapy is to prolong symptomatic efficacy without increasing peak plasma concentrations above the threshold for dyskinesias. Levodopa, carbidopa, and entacapone (LCE) is a combination medication that enhances levodopa delivery to the brain, leading to improved clinical efficacy. The safety and efficacy of LCE have been established through trials and experience.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Article
Chemistry, Multidisciplinary
Hui Jin, Jinyan Cui, Wei Zhan
Summary: A liposome-based micromotor system that achieves directional motion in water by using regional enzymatic conversion and gas generation is presented. These liposomes, composed of low-melting and high-melting lipids along with cholesterol, maintain stable Janus configuration through lipid liquid-liquid phase separation. Local placement of enzymes is achieved through affinity binding, resulting in enzyme-decorated liposomes that undergo directional motion in the presence of substrate hydrogen peroxide. Experimental details on liposome size control, motor assembly, and substrate distribution are provided, highlighting the importance of asymmetry in achieving particle directional motion.
Article
Biochemistry & Molecular Biology
Damian Tarasek, Beata Gasowska-Bajger, Hubert Wojtasek
BIOORGANIC CHEMISTRY
(2020)
Article
Chemistry, Organic
Adam Drop, Hubert Wojtasek, Bozena Frackowiak-Wojtasek
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2020)
Review
Medicine, General & Internal
Beata Gasowska-Bajger, Agnieszka Gasowska-Bodnar, Pawel Knapp, Lubomir Bodnar
Summary: The meta-analysis suggests that survivin expression may serve as an indicator of poor prognosis in ovarian cancer, and is associated with clinicopathological parameters of greater aggressiveness in the disease. Prospective studies are necessary to validate survivin expression as a potential biomarker for ovarian cancer.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Damian Tarasek, Hubert Wojtasek, Khedidja Benarous, Mohamed Yousfi
Summary: Gallic acid and hispidin were previously described as inhibitors of horseradish peroxidase, but additional experiments showed that both compounds are rapidly oxidized by the enzyme. Although it was interpreted as enzyme inhibition, the activity of horseradish peroxidase is not affected by these compounds.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Editorial Material
Chemistry, Multidisciplinary
Hubert Wojtasek
Summary: A review article discusses flavonoids as inhibitors of tyrosinase, but many of these compounds actually act as substrates of the enzyme or antioxidants, resulting in inhibition effects.
Editorial Material
Food Science & Technology
Hubert Wojtasek
Summary: The article describes Quercetin as an inhibitor of tyrosinase, but previous literature has firmly established it as a substrate of the enzyme and an antioxidant that reduces tyrosinase-generated o-quinones.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Physical
Damian Tarasek, Beata Gasowska-Bajger, Bozena Frackowiak-Wojtasek, Christian Kersten, Michal Jewginski, Lukasz Kolodziej, Rafal Latajka, Hubert Wojtasek
Summary: The study found that the phenol group in dobutamine is preferentially oxidized by horseradish peroxidase (HRP), with a faster oxidation rate compared to dopamine. This difference is primarily attributed to molecular configuration and K-m values, with the distance between the oxidizable group and the heme cofactor determined by interactions with functional groups in the side chain and the peptide backbone near the active site.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Damian Tarasek, Hubert Wojtasek
Summary: Rifampicin has been found to be both an inhibitor of tyrosinase and can be oxidized by tyrosinase-generated o-quinones. Its absorption overlaps with dopachrome and does not affect the actual reaction rates.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Spectroscopy
Joanna Nackiewicz, Beata Gasowska-Bajger, Lukasz Kolodziej, Anna Poliwoda, Kinga Pogoda-Mieszczak, Magdalena Skonieczna
Summary: This study compared the degradation process of diclofenac (DCF) by hematoprotein myeloperoxidase (MPO) and iron octacarboxyphthalocyanine (FePcOC) in the presence of hydrogen peroxide. It was found that FePcOC and hydroxyl radicals (from H2O2) resulted in the formation of an intermediate product (dimer with an m/z value of 587) with a yellow color and intense absorption at 451 nm. Similarly, MPO and hydrogen peroxide also generated yellow solutions with an absorption maximum at 451 nm, but LC-MS/MS analysis revealed at least seven main products of the diclofenac oxidation process. The degradation mechanism of diclofenac with MPO is more complex than with FePcOC.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Beata Gasowska-Bajger, Hubert Wojtasek
Summary: Baicalein is described as an inhibitor of tyrosinase, but it is actually a substrate of this enzyme, reducing the lag-phase during oxidation of L-tyrosine. Other compounds with a 1,2,3-triphenol moiety can also be oxidized by tyrosinase. Baicalein is rapidly oxidized by o-quinones generated from catechols by tyrosinase or by treatment with sodium periodate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Letter
Biochemistry & Molecular Biology
Hubert Wojtasek
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Bozena Frackowiak-Wojtasek, Beata Gasowska-Bajger, Damian Tarasek, Martyna Mytnik, Hubert Wojtasek
Summary: The study found that anti-thyroid drugs and their selenium analogs have inhibitory effects on the oxidation of ABTS by lactoperoxidase, which may explain the impact of other compounds and natural antioxidants on thyroid function.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
