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Agriculture, Dairy & Animal Science
Q. Li, X. Du, L. Liu, H. Liu, Z. Pan, Q. Li
Summary: miR-146b is a novel epigenetic factor that regulates GC functions, follicular development, and female reproduction in pigs by targeting CYP19A1 for E2 synthesis signaling.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huiming Wang, Yi Zhang, Jinbi Zhang, Xing Du, Qifa Li, Zengxiang Pan
Summary: This study discovered that circSLC41A1 enhances SRSF1 expression through competitive binding of miR-9820-5p and plays a role in follicular GC apoptosis regulation. It provides insights into the post-transcriptional regulation of follicular atresia and the protein-coding function of circRNA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Wang Yao, Zengxiang Pan, Xing Du, Jinbi Zhang, Honglin Liu, Qifa Li
Summary: The study identified NORHA as a highly expressed lncRNA in atretic follicles, inducing granulosa cell apoptosis by influencing the activities of the miR-183-96-182 cluster and FoxO1 axis. This comprehensive perspective sheds light on lncRNA regulation of follicular atresia.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Hongbing Gui, Fan Li, Cheng Chen, Qiuyi Zhu, Chenjian Zhang, Jun Zhang, Chunhua Meng, Yong Qian, Shaoxian Cao, Yinxia Li
Summary: Our experiments with granulosa cells from Hu sheep revealed that miR-27a-3p is involved in regulating E2 synthesis and is associated with ovine litter size. miR-27a-3p inhibits CYP19A1 expression and E2 synthesis by targeting NR5A2. We also identified single nucleotide polymorphisms in the ovine miR-27a gene that are significantly associated with litter size.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Shunshun Han, Jianping Wang, Can Cui, Chunlin Yu, Yao Zhang, Diyan Li, Menggen Ma, Huarui Du, Xiaosong Jiang, Qing Zhu, Chaowu Yang, Huadong Yin
Summary: This study explores the regulatory molecular mechanisms of follicular atresia in chickens and reveals the role of autophagy and apoptosis in this process. It also highlights the importance of fibromodulin in regulating autophagy and provides insights into broodiness and poultry production improvement.
Article
Reproductive Biology
Xinyan Zhang, Tong Yu, Xinyan Guo, Ruixue Zhang, Yanni Jia, Chunmei Shang, Aihua Wang, Yaping Jin, Pengfei Lin
Summary: This study reveals the important role of ufmylation in granulosa cell apoptosis during goat follicular atresia, with a preventive effect on ER stress-induced apoptosis but no impact on oxidative stress-induced apoptosis.
Article
Biochemistry & Molecular Biology
Mengnan Ma, Huiming Wang, Yi Zhang, Jinbi Zhang, Jingge Liu, Zengxiang Pan
Summary: The study identified that ssc-circINHA-001 plays a role in resisting GC apoptosis by enhancing INHBA expression through adsorbing miR-214-5p, miR-7144-3p, and miR-9830-5p. Low expression of ssc-circINHA-001 increases free miRNAs, inhibits INHBA expression, and leads to GC apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Su Zhou, Yueyue Xi, Yingying Chen, Fangfang Fu, Wei Yan, Milu Li, Yaling Wu, Aiyue Luo, Ya Li, Shixuan Wang
Summary: This study revealed that WIP1 expression is significantly lower in atretic follicle granulosa cells and appropriate WIP1 expression is essential for healthy follicular development. Inhibiting WIP1 activity accelerates ovarian aging by promoting follicular atresia and primordial follicle activation, leading to decreased ovarian reserve.
Article
Oncology
Xingyu Tao, Yang Li, Songqing Fan, Liyang Wu, Jianyang Xin, Yun Su, Xiaoyang Xian, Yingying Huang, Rongquan Huang, Weiyi Fang, Zhen Liu
Summary: This study aimed to investigate the role of LINC00173 in the chemosensitivity of lung adenocarcinoma (LUAD) to cisplatin. The results showed that downregulated expression of LINC00173 in cisplatin-resistant LUAD led to chemoresistance by regulating the miR-1275/PROCA1/ZFP36L2/BCL2 pathway to induce cell apoptosis and reduce the apoptotic signal.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Cell Biology
Shengjie Shi, Yamei Hu, Xiangrong Song, Liang Huang, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: miR-184 plays a key role in the physiological functions of granulosa cells by promoting E-2 synthesis and cell proliferation, and inhibiting apoptosis through targeting three genes.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Cell Biology
Juan Dong, Changquan Guo, Shuo Zhou, An Zhao, Jian Li, Yuling Mi, Caiqiao Zhang
Summary: This study found that leukemia inhibitory factor (LIF) can inhibit autophagic cell death of granulosa cells by activating the PI3K/AKT and Stat3 signaling pathways, thus relieving granulosa cell apoptosis and promoting follicle development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Anthony Nzioka, Ainara Valencia, Aitor Atxaerandio-Landa, Oihane Diaz de Cerio, Mohammad Amzad Hossain, Maria Korta, Maren Ortiz-Zarragoitia, Ibon Cancio
Summary: This study investigated genes related to apoptosis and autophagy during follicular atresia in European hake. The results showed different implications of apoptotic and autophagic processes during oocyte development. The analyzed genes could serve as biomarkers for early detection of follicular atresia and evaluation of fecundity in fish stocks.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Reproductive Biology
Ke Hu, Chen Wang, Yifan Xu, Fan Li, Xuefeng Han, Chuanwang Song, Meng Liang
Summary: The functions and mechanisms of lncRNAs in reproduction are extensively studied, but the regulation of hormone synthesis in ovarian follicular granulosa cells by lncRNAs is not well understood. This study identified the interaction between lncRNA Gm2044 and the EEF2 protein, and demonstrated that lncRNA Gm2044 promotes estradiol synthesis in follicular granulosa cells. Knockout mice experiments further confirmed the role of lncRNA Gm2044 in regulating estradiol synthesis. Understanding the mechanism of lncRNA Gm2044/EEF2 protein in estradiol synthesis can have implications for treating estrogen-related reproductive diseases.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Reproductive Biology
Jiarui Cheng, Yaochang Wei, Ziwen Zhao, Qinghua Xing, Ziyan Gao, Juanru Cheng, Chengqi Yu, Yu Pan, Yanyan Yang, Deshun Shi, Yanfei Deng
Summary: MiR-29c-5p promotes apoptosis, inhibits proliferation and steroidogenesis in buffalo granulosa cells by targeting INHBA, ultimately leading to atresia in buffalo follicles.
Article
Environmental Sciences
Haohao Liu, Zhihui Tian, Yaxin Guo, Xiaohui Liu, Ya Ma, Xingde Du, Rui Wang, Shiyu Zhang, Linjia Shi, Hongxiang Guo, Huizhen Zhang
Summary: The study found that MC-LR can cause female reproductive toxicity by inducing apoptosis and follicular atresia in ovarian cells. The endoplasmic reticulum stress plays a crucial role in the apoptotic pathway induced by MC-LR, where Ddit3 regulates cell apoptosis and follicular atresia by modulating other genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Q. Li, X. Du, L. Liu, H. Liu, Z. Pan, Q. Li
Summary: miR-146b is a novel epigenetic factor that regulates GC functions, follicular development, and female reproduction in pigs by targeting CYP19A1 for E2 synthesis signaling.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Wang Yao, Xing Du, Jinbi Zhang, Yang Wang, Miaomiao Wang, Zengxiang Pan, Qifa Li
Summary: This study identified BRE-AS as a crucial AS-lncRNA involved in granulosa cell apoptosis, potentially contributing to the initiation of follicular atresia. Through direct interaction with the pre-mRNA transcript of its overlapping gene BRE, BRE-AS induces granulosa cell apoptosis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Obstetrics & Gynecology
Wang Yao, Siqi Wang, Xing Du, Chenggang Lin, Jinbi Zhang, Zengxiang Pan, Qifa Li
Summary: This study characterized the promoter region of the porcine miR-183-96-182 cluster and identified SMAD4 as a potential transcriptional activator of this cluster in GCs through direct binding to SBE motifs. The study also revealed a potential regulatory pathway involving TGF-beta 1/SMAD4/miR-183-96-182 cluster/FoxO1 in controlling follicular atresia and female reproduction.
REPRODUCTIVE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Huiming Wang, Yi Zhang, Jinbi Zhang, Xing Du, Qifa Li, Zengxiang Pan
Summary: This study discovered that circSLC41A1 enhances SRSF1 expression through competitive binding of miR-9820-5p and plays a role in follicular GC apoptosis regulation. It provides insights into the post-transcriptional regulation of follicular atresia and the protein-coding function of circRNA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Liu Yang, Siqi Wang, Zengxiang Pan, Xing Du, Qifa Li
Summary: The deubiquitinase USP9X positively regulates the expression of TGFBR2, a key component of the TGF-beta signaling pathway, in granulosa cells. This activation of the TGF-beta pathway by USP9X leads to the suppression of granulosa cell apoptosis and plays a crucial role in female fertility.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jiying Liu, Ruilong Song, Shengyan Su, Nannan Qi, Qifa Li, Zhuang Xie, Shali Yu
Summary: Betaine can improve fat distribution and mitochondrial function in goose liver, with a certain repairing effect in a high-glucose culture medium, reducing fatty liver damage.
Article
Biochemistry & Molecular Biology
Siqi Wang, Yuqi Li, Qiang Zeng, Liu Yang, Xing Du, Qifa Li
Summary: This study reported the influence of a mutation in miR-23a on the response of granulosa cells (GCs) to oxidative stress. The findings revealed the genetic basis of individual differences in the response to oxidative stress and identified miR-23a as a candidate gene for environmental adaptation to oxidative stress.
Article
Cell Biology
Miaomiao Wang, Yang Wang, Wang Yao, Xing Du, Qifa Li
Summary: This study identifies a novel long noncoding RNA (lncRNA) lnc2300 that regulates the miR-365-3p/CYP11A1 signaling pathway, and contributes to the regulation of granulosa cell (GC) apoptosis during ovarian follicular atresia.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Siqi Wang, Yuqi Li, Xing Du, Qifa Li
Summary: This study reveals that the two SNVs in the miR-23a promoter have significant effects on granulosa cell apoptosis and may play a crucial role in regulating female fertility. miR-23a has the potential to be a small-molecule nonhormonal drug for treating female fertility issues.
Article
Biochemistry & Molecular Biology
Siqi Wang, Yang Wang, Yibo Chen, Yuqi Li, Xing Du, Yinxia Li, Qifa Li
Summary: MiR-23a and NORHA are repressed by a common transcription factor MEIS1, which forms a small network regulating sow GC apoptosis. MEIS1 inhibits the transcription activity of miR-23a and NORHA by directly binding to their core promoters, and represses GC apoptosis by inhibiting the miR-23a/NORHA axis downstream target FoxO1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yangan Huo, Qiqi Li, Liu Yang, Xiaoxue Li, Chen Sun, Yang Liu, Honglin Liu, Zengxiang Pan, Qifa Li, Xing Du
Summary: Increasing evidence shows that lncRNAs are involved in the regulation of follicular development and female fertility, but the mechanism remains largely unknown. This study reveals that the lncRNA SDNOR is a potential regulator in porcine follicular granulosa cells, inhibiting the transcription factor SOX9 and affecting downstream targets. Loss of SDNOR impairs GC morphology, inhibits cell proliferation and viability, reduces E2/P4 index, and suppresses the expression of crucial markers. Additionally, SDNOR elevates the resistance of GCs to oxidative stress and inhibits apoptosis induced by oxidative stress.
Article
Cell Biology
Liu Yang, Xing Du, Siqi Wang, Chenggang Lin, Qiqi Li, Qifa Li
Summary: miR-187 maintains TGFBR2 mRNA stability in sow GCs by activating the TGF-beta signaling pathway and inhibiting GC apoptosis. NORHA inhibits the activation of TGF-beta signaling pathway by sponging miR-187. NORFA enhances miR-187 and TGFBR2 expression by inhibiting NORHA and activating NFIX.
CELL DEATH DISCOVERY
(2023)
Article
Biology
Miaomiao Wang, Yang Wang, Liu Yang, Xing Du, Qifa Li
Summary: This study reveals a novel mechanism of action for nuclear lncRNAs and shows that it acts as a competing endogenous RNA (ceRNA), which is also a common function of lncRNAs in the cytoplasm. The study also identifies a potential endogenous small activating RNA (saRNA) for improving female fertility and treating female infertility.
Article
Cell Biology
Xing Du, Lu Liu, Wangjun Wu, Pinghua Li, Zengxiang Pan, Lifan Zhang, Jiying Liu, Qifa Li
JOURNAL OF CELL SCIENCE
(2020)
Article
Biology
Xing Du, Lu Liu, Qiqi Li, Lifan Zhang, Zengxiang Pan, Qifa Li
COMMUNICATIONS BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Damien Marchese, Florent Guislain, Tamara Pringels, Laure Bridoux
Summary: Homopolymeric amino acid repeats are common in human proteins, particularly in transcription factors and kinases. This study focuses on homopolymeric histidine repeats (polyH) and their role in regulating embryonic development. Through bioinformatic analysis, the study identifies that polyH-containing proteins interact with cysteine-rich proteins and proteins containing cysteine repeats. The study further investigates the HOXA1 protein, a transcription factor with a long polyH motif, and finds that the polyH motif is necessary for its interaction with cysteine-rich proteins. Additionally, the study discovers that metal ions are required for the HOXA1-MDFI interaction and identifies three polyH interactors that down-regulate the transcriptional activity of HOXA1.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2024)